RESUMO
Clostridioides difficile infection (CDI) has been increasingly observed in children, but there is a lack of epidemiological and molecular data on CDI in Latin America. This prospective cohort study aimed to investigate the role of CDI in children with diarrhea. It included 105 children with antimicrobial-associated diarrhea (AAD) and analyzed the molecular characteristics of strains isolated from two hospitals in southern Brazil between 2017 and 2020. Fecal samples from the participants were tested for glutamate dehydrogenase (GDH) and A/B toxins using a rapid enzyme immunoassay. GDH-positive samples underwent automated real-time polymerase chain reaction and toxigenic culture. Toxigenic C. difficile isolates were selected for whole genome sequencing. Out of the 105 patients, 14 (13.3%) met the criteria for CDI. Children with a history of previous CDI and the presence of mucus in their stool were more likely to have CDI. Metronidazole was the most used treatment (71.4%), and three patients (23.1%) experienced CDI recurrence (rCDI). Although the number of sequenced isolates was limited, a wide diversity of sequence types (ST) was observed. In addition to toxin genes (tcdA, tcdB, cdtA, and cdtB), the isolates also exhibited virulence factors involved in adhesion (cwp66, groEL, slpA, fbpA/fbp68) and immune evasion (rmlA, rmlB, rmlC, gnd, rfbA-1), along with multiple resistance factors (gyrA mutation, norA, ermB, dfrF, and vanG). These findings highlight the prevalence and recurrence of CDI among hospitalized children. Longitudinal studies are needed to better understand the characteristics of CDI-associated diarrhea and its impact on the healthcare system in this population.
Assuntos
Toxinas Bacterianas , Clostridioides difficile , Infecções por Clostridium , Humanos , Criança , Toxinas Bacterianas/genética , Clostridioides difficile/genética , Brasil/epidemiologia , Estudos Prospectivos , Proteínas de Bactérias/genética , Proteínas de Bactérias/análise , Infecções por Clostridium/epidemiologia , Hospitais , Diarreia/epidemiologiaRESUMO
Invasive candidiasis (IC) contributes to the morbidity and mortality of hospitalized patients and represents a significant burden to the healthcare system. Previous Brazilian studies have reported the presence of endemic Candida parapsilosis sensu stricto genotypes causing candidemia and clonal transmission involving fluconazole-resistant isolates. We performed a 5-year retrospective analysis of IC cases in a Brazilian tertiary pediatric hospital and conducted a molecular investigation of C. parapsilosis sensu stricto. Non-duplicate C. parapsilosis sensu stricto genotyping was performed by microsatellite analysis. Antifungal susceptibility and biofilm formation were also evaluated. A total of 123 IC episodes were identified, with an IC incidence of 1.24 cases per 1000 hospital admissions and an overall mortality of 34%. The main species were the C. parapsilosis complex (35.8%), Candida albicans (29.2%), and Candida tropicalis (21.9%). All C. parapsilosis sensu stricto were recovered from blood cultures, and 97.5% were biofilm producers. Microsatellite typing identified high genotypic diversity among the isolates. We observed that all isolates were sensitive to amphotericin B, and although one isolate was non-sensitive to fluconazole, only a silent mutation on ERG11 gene was identified. No clear evidence of clonal outbreak or emergence of fluconazole-resistant isolates was found, suggesting that multiple sources may be involved in the epidemiology of IC in children.
RESUMO
OBJECTIVE: To describe the evolution of out-of-home food consumption in Brazil in 2008-2018. METHODS: We used the 2008-2009 and 2017-2018 data from the Inquéritos Nacionais de Alimentação (INA - National Food Surveys), conducted amid 34,003 and 46,164 individuals, to estimate the frequency of out-of-home food consumption and the contribution of this consumption to specific foods. Food consumption was analyzed using food records in the 2008-2009 INA and 24-hour recalls in 2017-2018. Estimates were generated for Brazil in general, for urban and rural areas, for age groups (adolescent, adult, elderly), and for income bracket. RESULTS: The frequency of out-of-home consumption decreased by 8.8% between the two surveys, with no change in the rural area, in the Northeast and South regions, and for the lowest income brackets. We observed a slight increase among the elderly and in the Midwest region. The contribution of out-of-home food consumption to daily energy intake also decreased (16.3% vs. 12.7%), excepting the rural area, where there was a reduction in the difference in relation to the urban area between the two surveys. For most items evaluated, the out-of-home food consumption decreased. The most consumed out-of-home food were alcoholic beverages, fried and baked snacks, soft drinks, pizza, sweets, and sandwiches in both surveys. CONCLUSION: In 10 years, the prevalence of food consumption and the percentage of contribution of out-of-home food decreased in Brazil, but ultra-processed foods still figure as the most consumed food group outside the home.
Assuntos
Comportamento Alimentar , Lanches , Adolescente , Adulto , Idoso , Brasil , Dieta , Inquéritos sobre Dietas , Ingestão de Energia , Fast Foods , Alimentos , HumanosRESUMO
ABSTRACT OBJECTIVE To describe the evolution of out-of-home food consumption in Brazil in 2008-2018. METHODS We used the 2008-2009 and 2017-2018 data from the Inquéritos Nacionais de Alimentação (INA - National Food Surveys), conducted amid 34,003 and 46,164 individuals, to estimate the frequency of out-of-home food consumption and the contribution of this consumption to specific foods. Food consumption was analyzed using food records in the 2008-2009 INA and 24-hour recalls in 2017-2018. Estimates were generated for Brazil in general, for urban and rural areas, for age groups (adolescent, adult, elderly), and for income bracket. RESULTS The frequency of out-of-home consumption decreased by 8.8% between the two surveys, with no change in the rural area, in the Northeast and South regions, and for the lowest income brackets. We observed a slight increase among the elderly and in the Midwest region. The contribution of out-of-home food consumption to daily energy intake also decreased (16.3% vs. 12.7%), excepting the rural area, where there was a reduction in the difference in relation to the urban area between the two surveys. For most items evaluated, the out-of-home food consumption decreased. The most consumed out-of-home food were alcoholic beverages, fried and baked snacks, soft drinks, pizza, sweets, and sandwiches in both surveys. CONCLUSION In 10 years, the prevalence of food consumption and the percentage of contribution of out-of-home food decreased in Brazil, but ultra-processed foods still figure as the most consumed food group outside the home.
RESUMO OBJETIVO Descrever a evolução do consumo de alimentos fora do domicílio no Brasil no período 2008-2018. MÉTODOS Foram usados dados dos Inquéritos Nacionais de Alimentação (INA) de 2008-2009 e 2017-2018, realizados com 34.003 e 46.164 indivíduos, respectivamente, para estimar a frequência de consumo de alimentos fora de casa e a contribuição desse consumo para alimentos específicos. O consumo de alimentos foi investigado por meio de registros alimentares no INA 2008-2009 e de recordatórios 24 horas em 2017-2018. As estimativas foram geradas para o Brasil como um todo, para áreas urbana e rural, para faixas de idade (adolescente, adulto, idoso) e para faixas de renda. RESULTADOS A frequência de consumo fora de casa diminuiu 8,8% entre os dois inquéritos, sem alteração na área rural, nas regiões Nordeste e Sul e nas menores faixas de renda. Houve leve aumento entre idosos e na região Centro-Oeste. De modo geral, a contribuição do consumo alimentar fora de casa para a ingestão diária de energia também diminuiu (16,3% vs. 12,7%), com exceção da área rural, onde houve redução da diferença em relação à área urbana entre os dois inquéritos. O consumo de alimentos fora de casa foi reduzido para a maioria dos itens avaliados. Os alimentos mais consumidos fora de casa em ambos os inquéritos foram as bebidas alcoólicas, salgadinhos fritos e assados, refrigerantes, pizzas, doces e sanduíches. CONCLUSÃO Em 10 anos, a prevalência de consumo de alimentos e o percentual de contribuição dos alimentos consumidos fora de casa diminuíram no Brasil, mas os alimentos ultraprocessados ainda figuram como o grupo de alimento mais consumido fora de casa.