RESUMO
OBJECTIVE: Considering that hMSH2, hMLH1 and p53 are important in maintaining genomic stability of the oral mucosa epithelium, the purpose of the present study was to investigate the immunolocalization of these proteins in the epithelium of the oral mucosa of patients submitted to bone marrow transplantation (BMT) compared with controls. MATERIALS AND METHODS: Twenty-one samples of lip biopsies from BMT recipients were retrieved. Twenty samples of normal lower labial mucosa associated with mucocele in non-transplanted patients were included as control group. The streptavidin-biotin complex stain was used to detect the human DNA mismatch repair proteins hMSH2, hMLH1 and p53 protein. RESULTS: The main findings demonstrated that the mean number of suprabasal epithelial cells positive for MSH2 was statistically higher than the control group. The immunostaining of hMLH1 and p53 at the basal and suprabasal epithelial layers were statistically higher in the oral labial mucosa of the BMT patients compared with controls. CONCLUSION: The present study shows that oral epithelial cells of BMT patients show increased immunolocalization of the DNA repair related proteins.
Assuntos
Transplante de Medula Óssea/fisiologia , Enzimas Reparadoras do DNA/biossíntese , Proteínas de Ligação a DNA/biossíntese , Mucosa Bucal/metabolismo , Proteínas de Neoplasias/biossíntese , Proteínas Proto-Oncogênicas/biossíntese , Proteína Supressora de Tumor p53/biossíntese , Proteínas Adaptadoras de Transdução de Sinal , Adolescente , Pareamento Incorreto de Bases , Proteínas de Transporte , Estudos de Casos e Controles , Enzimas Reparadoras do DNA/análise , Proteínas de Ligação a DNA/análise , Células Epiteliais/química , Células Epiteliais/metabolismo , Feminino , Humanos , Técnicas Imunoenzimáticas , Masculino , Pessoa de Meia-Idade , Mucosa Bucal/química , Proteína 1 Homóloga a MutL , Proteína 2 Homóloga a MutS , Proteínas de Neoplasias/análise , Proteínas Nucleares , Proteínas Proto-Oncogênicas/análise , Proteína Supressora de Tumor p53/análiseRESUMO
The purpose of this study was to evaluate cervical mistifing of casting full metal crowns according to investment techniques using commercial available alloys made from silver stannum. Fifteen metal full crowns were obtained through stainless steel die with full crowns preparation, included into three groups as follows: conventional technique, vacuum technique and pressure technique. Readings of cervical disagreement were made through a comparing microscope increased 45 x and each crown was measured in 4 different regions (vestibular, lingual mesial and distal). This way it was verified which of the techniques proposed would balance the casting shrinkage for the silver-stannum alloy used. From the results obtained we conclude that the best cercical adaptation was the one got through pressure technique.
Assuntos
Coroas , Ligas Dentárias , Revestimento para Fundição Odontológica , Técnica de Fundição Odontológica , Infiltração Dentária/etiologia , Prata , EstanhoRESUMO
The work aims to investigate the influence of positive pressure to initial and final setting time, as well as the volume variation in course of the pressure carried out gypsum-bonded investment found at Dental Commerce. Seventy-six test samples were obtained, being five of them through conventional investing technique and seventy-one through technique by pressure. Gillmore needles were employed for determining the setting time, meanwhile for volumetric variation a mathematic formula had been applied. According to results, it was concluded that the use of positive pressure affected the initial and the final setting time and the volume after set.