RESUMO
A study was carried out with the objective of determining the presence of Bacillus cereus in eggshells commercialized in Mexico, the enterotoxigenic profile of the isolated strains, and the production of biofilms in different materials as well as in the eggshell. 1000 chicken eggs from four commercial brands were collected from markets and supermarkets located in the city of Chilpancingo, Mexico. Bacillus cereus was isolated from the eggshell. The molecular identification was by amplification of the gyrB gene and the enterotoxigenic profiles by the amplification of the cytK, ces, nheABC, and hblABD genes, in addition to the amplification of the tasA and sipW genes associated with the production of biofilms. In different materials and in eggshells, the production of biofilms was evaluated. The microbiological and molecular analysis of B. cereus yielded a frequency of 5.5% (55/1000), this was higher in brand III (11.6%, p=0.0001) and white eggshell (7.6%, 38/500, p≤0.001) and by marketing source, it was similar between market (5.2% / 26/500) and supermarket (5.8%, 29/500). The most common was the toxigenic profile A (23/55). Biofilm production is high in PVC in relation to other materials (p<0.0001), and the frequency of the related genes tasA and sipW was 72.7% and 40% respectively; the highest production was related to the tasA gene; in eggshell, most of the strains (54/55) were able to produce biofilm. Strains of B. cereus with toxigenic potential circulate and persist in this product, which shows the need for sanitary regulation in the country.(AU)
Assuntos
Bacillus cereus , Biofilmes , Casca de Ovo/microbiologia , EnterotoxinasRESUMO
El incremento de la rigidez arterial está asociado con el desarrollo de enfermedades cardiovasculares, las cuales constituyen una de las principales causas de muertes en el mundo. Por este motivo el desarrollo de métodos no invasivos que permitan cuantificar la rigidez arterial ha alcanzado un gran impacto. En este trabajo se estudia el método no invasivo de medición de la velocidad de la onda del pulso de la arteria braquial al tobillo (baPWV), por sus siglas en inglés. Para estudiar este método se simularon las formas de ondas de presión en el sistema arterial empleando un modelo unidimensional, a partir de las cuales se determinaron los valores de baPWV. Estos valores fueron comparados con los calculados por otros dos métodos: cfPWV (velocidad de la onda del pulso entre la carótida y la femoral, el método estándar) y PWVteor (ecuación de Bramwell-Hill), obteniéndose correlaciones significativas, r=0.967 y r=0.9828 respectivamente. Se investigó la sensibilidad del método baPWV a la variación de la rigidez, representada por la variación de la distensibilidad y, se concluyó que el método es sensible a los cambios de rigidez que ocurren tanto en las arterias centrales como en las arterias periféricas.
The arterial stiffness increased is associated with the development of cardiovascular diseases, which constitute one of the first causes of death globally. For this reason the development of noninvasive methods to quantify arterial stiffness have had great impact. The purpose of this paper is the study of the noninvasive measurement method of brachial ankle pulse wave velocity (baPWV). To perform this study pressure waveforms in the arterial system were simulated, by using a one-dimensional model. With these pressure waveforms baPWV's values were calculated, and were compared with two others calculated methods: cfPWV (carotid-femoral PWV, gold standard method), and PWVteor (Bramwell-Hill equation). Significant correlations were obtained, r=0.967 y r=0.9828 respectively. The sensibility of the baPWV method to the stiffness change, represented for the distensibility change, was investigated, and we conclude that baPWV method is sensitive to the changes that take place in both central and peripheral arteries.
RESUMO
BACKGROUND: Choledocholithiasis presents in 5-10% of the patients with biliary lithiasis. Numerous treatment algorithms have been considered for this disease, however, up to 10% of these therapeutic procedures may fail. Intraoperative choledochoscopy has become a useful tool in the treatment of patients with difficult-to-manage choledocholithiasis. OBJECTIVES: To determine the usefulness of intraoperative choledochoscopy in the laparoendoscopic treatment of difficult stones that was carried out in our service. PATIENTS AND METHODS: A cross-sectional study was conducted. The case records were reviewed of the patients that underwent intraoperative choledochoscopy during biliary tree exploration plus laparoscopic choledochoduodenal anastomosis within the time frame of March 1, 2011 and May 31, 2012, at the Hospital General Dr. Manuel Gea González. Transabdominal choledochoscopies were performed with active stone extraction when necessary, followed by peroral choledochoscopies through the recently formed bilioenteric anastomosis. The data were analyzed with descriptive statistics and measures of central tendency. RESULTS: The mean age was 71 years, 57% of the patients were women, and the ASA III score predominated. Active extraction of stones with 7 to 35mm diameters was carried out in 4 of the cases and the absence of stones in the biliary tract was corroborated in all the patients. The mean surgery duration was 18 minutes (range: 4 to 45min). CONCLUSIONS: Choledochoscopy is a safe and effective minimally invasive procedure for the definitive treatment of difficult stones.
Assuntos
Sistema Biliar/patologia , Coledocolitíase/patologia , Coledocolitíase/cirurgia , Endoscopia do Sistema Digestório/métodos , Cirurgia Assistida por Computador/métodos , Humanos , Período Intraoperatório , Laparoscopia/métodosRESUMO
OBJECTIVE: To assess the impact of different doses of anti-interferon gamma (anti-IFNγ) F(ab')2 fragments, administered prophylactically, on survival and on serum concentration of cytokines in a murine model of sepsis induced by cecal ligation and puncture (CLP). We further explore the impact of therapeutic administration of the most protective dose on survival. SUBJECTS AND TREATMENT: Balb/c mice were prophylactically treated by the intraperitoneal route with anti-IFNγ initiated 2 h before CLP and every 24 h for a total of five times in each of the following doses: 0.01, 0.1, or 1 mg/kg. Sham and control groups received sterile saline solution in a similar scheme. METHODS: Serum tumor necrosis factor (TNF), interleukin (IL)-1ß, IL-6, IL-10 and IFNγ were measured at 3, 24 and 48 h after CLP by ELISA. Survival curves were compared using a Mantel-Haenzel method. RESULTS: Significant prophylactic protection was found only with 0.01 mg/kg, in association with regulation of IL-1ß and IL-10 concentrations. As therapy, anti-IFNγ fragments were protective only when initiated 24 h after CLP. CONCLUSIONS: Delicate modulation of IFNγ at the correct timing, even when the septic process has begun, is an exciting alternative to explore in the treatment of sepsis.
Assuntos
Fragmentos de Imunoglobulinas/química , Interferon gama/metabolismo , Sepse/imunologia , Animais , Modelos Animais de Doenças , Ensaio de Imunoadsorção Enzimática/métodos , Humanos , Interferon gama/biossíntese , Interleucina-10/biossíntese , Interleucina-1beta/biossíntese , Interleucina-6/biossíntese , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Proteínas Recombinantes/metabolismo , Sepse/patologia , Fatores de TempoRESUMO
Children and teenagers with intellectual disability are a complex issue within the society and for the medical practice. Determining the more frequent forms of abuse, it's behaving in this particular population, and to evaluate the possible relationship between juvenile abuse and psychiatric co-morbidities were the main objectives in this investigation. 99 people with intellectual disability between 5 and 17 year old from three different communities (Felicia, Cocosol and Belen) located at Marianao municipality were selected by monoetapic conglomerate and studied firstly trough an analytic study with transversal cut seconded by a differentiated intervention according with the results. Taking into account the sociopsychiatric history, the psychiatric examination, using strictest observational techniques of the patient at home and the school, and applying the tools for diagnosis of infant abuse in this people, we concluded that juvenile abuse was a serious health problem in the studied population: negligence and carelessness the main types. The relationship between infant abuse and psychiatric co-morbities was demonstrated.
La condición de discapacidad intelectual convierte a niños y adolescentes en población de alta complejidad en la medicina. Determinar comportamiento y formas más frecuentes del maltrato en esta población, así como evaluar la relación entre maltrato infantil y comorbilidad psiquíatrica, fueron los objetivos fundamentales de esta investigación. Para ello se estudió una muestra de 99 pacientes discapacitados intelectuales en edades comprendidas entre 5 y 17 años de tres consejos populares (Felicia, Cocosol y Belén) del municipio Marianao, seleccionados por conglomerado monoetápico. Se realizó un estudio analítico de corte transversal en un primer momento, con intervención diferenciada según los resultados en un segundo momento. Mediante la historia socialpsiquíatrica, examen psiquiátrico, empleo de técnicas observacionales rigurosas del paciente en la casa y escuela y aplicación de instrumento para el diagnóstico de maltrato infantil en este tipo de población. Concluimos que el maltrato infantil fue un problema de salud en la muestra estudiada; el maltrato por negligencia o descuido fueron los más frecuentes. Se comprobó estrecha relación entre la existencia de maltrato infantil y comorbilidad psiquiátrica en los pacientes estudiados.
Assuntos
Humanos , Pessoas com Deficiência Mental , Maus-Tratos Infantis/diagnóstico , Estudos Transversais , Chile/epidemiologia , Diagnóstico Duplo (Psiquiatria) , Inquéritos e QuestionáriosRESUMO
BACKGROUND: Educational strategies look for the increase of knowledge in physicians; they are a useful resourse for the diffusion among physicians of guides GINA and ARIA. OBJECTIVE: To evaluate a course shop-like for physicians as an educative strategy. PARTICIPANTS AND METHODS: A transversal study was performed, where knowledge was evaluated to primary contact physicians about recent currents of guides of GINA and ARIA 2006. RESULTS: There was a participation of 69 primary contact physicians who applied a questionnaire of 30 questions: 20 about asthma (GINA) and 10 about allergic rhinitis (ARIA) before and after a course shop-like for physicians; there was improvement on calification after educative strategy on knowledge about asthma and allergic rhinitis with a p = < 0.05. CONCLUSION: The educative strategy proposed as course shop-like for primary contact physicians is effective for teaching the guides of GINA and ARIA 2006.
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Asma/terapia , Competência Clínica , Medicina de Família e Comunidade , Médicos , Guias de Prática Clínica como Assunto , Rinite Alérgica Perene/terapia , Rinite Alérgica Sazonal/terapia , Adulto , Estudos Transversais , Feminino , Humanos , MasculinoRESUMO
We present in this work the synthesis and cardiovascular effects of new methylthiomorpholine compounds and they were compared with cardiovascular drugs such as captopril, losartan and omapatrilat.