RESUMO
The number of myenteric neurons may be reduced by topical serosal application of benzalkonium chloride (BAC). We studied the effects of ageing in the population of neurons that survive after the application of BAC. Ten treated and ten control animals were killed at intervals of 2, 6, 12 and 18 months after the surgery. We performed myenteric neurons counting in serially cut histological preparations of the descending colon. The control animals revealed a continuous loss of myenteric neurons number with increasing of age. Interestingly, contrary to control animals, the BAC-treated rats presented no neuron loss with ageing at any experimental time. The reasons for their survival with ageing could be related to a neuroplasticity phenomenon.
Assuntos
Envelhecimento , Compostos de Benzalcônio/farmacologia , Plexo Mientérico/efeitos dos fármacos , Neurônios/citologia , Neurônios/efeitos dos fármacos , Animais , Contagem de Células , Colo Sigmoide/efeitos dos fármacos , Colo Sigmoide/inervação , Masculino , Plexo Mientérico/citologia , Ratos , Ratos WistarRESUMO
INTRODUCTION: The reimplantation of a complete tracheal segment (CTS) as an autograft or homograft often results in total or partial necrosis of the CTS. However, previous experiments suggested that when the CTS is first vascularized by the sternohyoid muscle (SM) 21 days before the reimplantation, the CTS can be reimplanted without ischemia or stenosis. However, it is not clear if the CTS requires 21 days to attain full revascularization. GOAL: This study aims to ascertain the minimum time necessary for revascularization of the 6 rings CTS by the SM allowing reimplantation free of ischemia. METHODS: Fifteen mongrel dogs, divided into 3 groups were submitted to 2 sequential surgical procedures. In the first procedure, a SM flap was used to envelop 6 rings CTS in all dogs. After periods of 21 days (group 1, n = 5), 14 days (group 2, n = 5) and 7 days (group 3, n = 5), the compound flaps (CTS and SM) were neovascularized. Each CTS was transected and mobilized from the trachea and then replaced again in its original site, as an autoflap. RESULTS: After a period of at least 60 days, none of dogs presented any respiratory alteration. Macroscopic and microscopic analyses demonstrated adequate viability and no stenosis. CONCLUSION: Our findings suggest that it is possible to neovascularize the CTS using a SM flap within a period as short as 1 week.
Assuntos
Neovascularização Fisiológica , Reimplante , Retalhos Cirúrgicos , Traqueia/irrigação sanguínea , Traqueia/transplante , Animais , Feminino , Masculino , Transplante AutólogoRESUMO
We report the second case of infection with Chromobacterium violaceum that occurred in Brazil. A farm worker living in the State of São Paulo presented fever and severe abdominal pain for four days. At hospitalization the patient was in a toxemic state and had a distended and painful abdomen. Chest X-ray and abdominal ultrasound revealed bilateral pneumonia and hypoechoic areas in the liver. The patient developed failure of multiple organs and died a few hours later. Blood culture led to isolation of C. violaceum resistant to ampicillin and cephalosporins and sensitive to chloramphenicol, tetracyclin, aminoglicosydes, and ciprofloxacin. Autopsy revealed pulmonary microabscesses and multiple abscesses in the liver. The major features of this case are generally observed in infections by C. violaceum: rapid clinical course, multiple visceral abscesses, and high mortality. Because of the antimicrobial resistance profile of this Gram-negative bacillus, for appropriate empirical antibiotic therapy it is important to consider chromobacteriosis in the differential diagnosis of severe community infections in Brazil.
Assuntos
Chromobacterium , Infecções por Bactérias Gram-Negativas/microbiologia , Adulto , Antibacterianos/uso terapêutico , Brasil/epidemiologia , Chromobacterium/efeitos dos fármacos , Chromobacterium/isolamento & purificação , Resistência Microbiana a Medicamentos , Evolução Fatal , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Infecções por Bactérias Gram-Negativas/epidemiologia , Humanos , Abscesso Hepático/diagnóstico , Abscesso Hepático/microbiologia , MasculinoAssuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Fertilidade , Rabdomiossarcoma Embrionário/diagnóstico , Rabdomiossarcoma Embrionário/tratamento farmacológico , Neoplasias do Colo do Útero/diagnóstico , Neoplasias do Colo do Útero/tratamento farmacológico , Adolescente , Feminino , Humanos , Gravidez , Resultado da GravidezRESUMO
The authors report a clinical case of a 60-year-old Caucasian man, with two episodes of arterial embolization in the lower limbs. A microscope investigation of the emboli revealed that they originated from fungal aortitis caused by Paracoccidioides brasiliensis. A review of aortic infections showed only one similar report of this rare clinical expression of blastomycosis. The authors suggest a routine postoperative search for emboli followed by culture and histopathology.
Assuntos
Aortite/microbiologia , Perna (Membro)/irrigação sanguínea , Paracoccidioides/isolamento & purificação , Paracoccidioidomicose/microbiologia , Artéria Poplítea , Tromboembolia/microbiologia , Angiografia , Aortite/diagnóstico por imagem , Embolectomia , Evolução Fatal , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Paracoccidioidomicose/diagnóstico por imagem , Tromboembolia/diagnóstico por imagem , Tromboembolia/cirurgia , Tomografia Computadorizada por Raios XRESUMO
We report on the clinical evolution of the Brazilian family with Ramon syndrome described by de Pina-Neto et al. [1986, Am J Med Genet 25:441-443]. Three members (patients IV-2, IV-18, and IV-19) have developed pigmentary changes in the retina and paleness of the optic disk. Patient IV-18 also has developed giant hypertrophy of the labia minora that, when examined histopathologically, was found to be due to neoplastic fibroblast and epithelial proliferation caused by a fibromatous process similar to that reported in the gingivae of the patients with this syndrome. Audiologic function of patient IV-2 was normal, and no skin lesions were detected. The articular signs and symptoms show that the affected relatives developed rheumatoid arthritis, which is currently inactive in patient IV-18, whereas patient IV-2 did not develop these alterations.
Assuntos
Fibroma/genética , Doenças Retinianas/genética , Neoplasias Vulvares/genética , Adolescente , Adulto , Artrite Juvenil/genética , Brasil , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patologia , Querubismo/genética , Epilepsia/genética , Feminino , Fibroma/patologia , Seguimentos , Hipertrofia Gengival/genética , Transtornos do Crescimento/genética , Humanos , Hipertricose/genética , Deficiência Intelectual/genética , Masculino , Doenças Retinianas/patologia , Síndrome , Neoplasias Vulvares/patologiaAssuntos
Adenocarcinoma/patologia , Dermatomiosite/etiologia , Disgerminoma/patologia , Ictiose/etiologia , Neoplasias Ovarianas/patologia , Síndromes Paraneoplásicas/etiologia , Adenocarcinoma/tratamento farmacológico , Adulto , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Biópsia , Criança , Dermatomiosite/diagnóstico , Disgerminoma/tratamento farmacológico , Evolução Fatal , Feminino , Humanos , Ictiose/diagnóstico , Neoplasias Ovarianas/tratamento farmacológico , Síndromes Paraneoplásicas/diagnósticoRESUMO
Axillary masses are uncommon alterations when detected as an isolated finding. We evaluated 31 patients with isolated axillary masses. Patients with alterations of the breasts or the upper limbs or with Ipsilateral chest lesions were excluded from the study. Nine patients had occult breast cancer, 5 of them in the contralateral breast. Seven had metastatic lymph nodes of non-ductal origin, and 1 had carcinoma of apocrine cells with metastasis to the axilla. Four patients had benign lymphadenopathy which disappeared spontaneously, and 4 others had ruptured infundibular follicular cyst, nodular fibromatosis, inflammatory tuberculous and inflammatory rheumatoid lymphadenitis. Five had an ectopic breast (2 with a fibroadenoma and 3 with fibrocystic changes). One patient had an axillary lipoma. The mean age of patients with malignant pathology was 55.1 +/- 10.9 years, and the mean age of patients with a benign pathology was 43.1 +/- 14.7 (P < 0.01). Chest X-ray and bilateral mammography are useful when the cause of the mass cannot be determined by taking a detailed history of neoplastic or infectious antecedents, by careful physical examination of the skin of the arms, trunk and neck, or by palpation of the breasts and thyroid. Fine needle aspiration biopsy distinguishes between benign and malignant pathologies. In cases of indeterminate neoplasia, complete axillary dissection for diagnosis is indicated.
Assuntos
Axila/patologia , Neoplasias/diagnóstico , Adulto , Idoso , Neoplasias da Mama/diagnóstico , Neoplasias da Mama/patologia , Carcinoma Ductal de Mama/diagnóstico , Carcinoma Ductal de Mama/secundário , Diagnóstico Diferencial , Feminino , Humanos , Pessoa de Meia-IdadeRESUMO
Two 46,XX true hermaphrodites and one XX male without genital ambiguities are reported. They coexist in two generations of the same pedigree, with paternal transmission and in the absence of SRY (sex-determining region, Y chromosome). These familial cases provide evidence to support the hypothesis that these disorders are alternative manifestations of the same genetic defect, probably an autosomal dominant mutation (with incomplete penetrance) or an X-linked mutation (limited by the presence of the Y chromosome).
Assuntos
Proteínas de Ligação a DNA/genética , Transtornos do Desenvolvimento Sexual/genética , Proteínas Nucleares , Fatores de Transcrição , Cromossomo X , Adulto , Sequência de Bases , Criança , Primers do DNA , Feminino , Genes Dominantes , Genitália Feminina/patologia , Genitália Feminina/cirurgia , Genitália Masculina/patologia , Humanos , Recém-Nascido , Cariotipagem , Masculino , Dados de Sequência Molecular , Mutação , Linhagem , Reação em Cadeia da Polimerase , Análise para Determinação do Sexo , Proteína da Região Y Determinante do Sexo , Cromossomo YRESUMO
A 12-year-old girl had been presenting a woody infiltration and erythema in the frontal region and on the entire left half of the face, leading to deformity of the nose and buccal fissure, and adenomegaly in a posterior cervical chain, for the last 18 months. Sinusitis was diagnosed and treated with antibiotics, and submitted to ethmoid sinusotomy, with no improvement. Several laboratory tests were made to find the correct diagnosis. An intradermal test for delayed hypersensitivity showed a positive reaction (5 mm) with necrosis for metabolic antigens for Conidiobolus. An oral mucosa biopsy showed a dense lymphohistiocytic infiltrate and focal points of necrosis. Gomori staining for fungi revealed countless wide, nonseptate hyphae. Amphotericin B was prescribed during 35 days, with no improvement. Terbinafine given orally was started in association with amphotericin B. Reduction of the lesions was observed 2 months later. No side effects were seen during 4 months of treatment.