RESUMO
The efficacy of double manual cleaning (DMC) with enzymatic detergent followed by alkaline detergent on biofilm removal on hinged surgical instruments was compared to automated cleaning. Biofilm-covered haemostatic forceps were divided into four groups: positive control (soaked in sterile water); DMC; DMC plus extra brushing of the inner hinge; and automated cleaning. All DMC, DMC plus brushing the hinge, and automated cleaning significantly (P < 0.001) reduced 94.8%, 99.8%, and 100% viable bacteria and 82.3%, 93.8%, and 95.1% residual protein, respectively, compared to positive control. DMC instruments had significantly more viable bacteria (P < 0.05) and residual protein (P < 0.01) than those in instruments subjected to DMC with hinge brushing and automated cleaning. However, there was no significant difference in residual protein between DMC with hinge brushing and automated cleaning. In sterilizing service units with no access to automated cleaning equipment, it is important to brush the inner hinge during manual cleaning, and DMC plus brushing the inner hinge could be considered a viable alternative for cleaning hinged surgical instruments.
Assuntos
Descontaminação , Detergentes , Biofilmes , Humanos , Instrumentos Cirúrgicos/microbiologiaRESUMO
BACKGROUND: Biofilm formation has been shown to be associated with damaged areas of endoscope channels. It was hypothesized that the passage of instruments and brushes through endoscope channels during procedures and cleaning contributes to channel damage, bacterial attachment and biofilm formation. AIM: To compare surface roughness and bacterial attachment in used and new endoscope channels in vivo and in vitro. METHODS: Surface roughness of 10 clinically used (retired) and seven new colonoscope biopsy channels was analysed by a surface profiler. For the in-vitro study, a flexible endoscope biopsy forceps was passed repeatedly through a curved 3.0-mm-diameter Teflon tube 100, 200 and 500 times. Atomic force microscopy was used to determine the degree of inner surface damage. The number of Escherichia coli or Enterococcus faecium attached to the inner surface of the new Teflon tube and the tube with 500 forceps passes in 1 h at 37oC was determined by culture. RESULTS: The average surface roughness of the used biopsy channels was found to be 1.5 times greater than that of the new biopsy channels (P=0.03). Surface roughness of Teflon tubes with 100, 200 and 500 forceps passes was 1.05-, 1.12- and 3.2-fold (P=0.025) greater than the roughness of the new Teflon tubes, respectively. The number of E. coli and E. faecium attached to Teflon tubes with 500 forceps passes was 2.9-fold (P=0.021) and 4.3-fold (P=0.004) higher compared with the number of E. coli and E. faecium attached to the new Teflon tubes, respectively. CONCLUSION: An association was found between endoscope usage with damage to the biopsy channel and increased bacterial attachment.
Assuntos
Aderência Bacteriana , Endoscópios/microbiologia , Enterococcus faecium/fisiologia , Contaminação de Equipamentos/prevenção & controle , Escherichia coli/fisiologia , Biofilmes/crescimento & desenvolvimento , Desinfecção/métodos , Politetrafluoretileno , Propriedades de SuperfícieRESUMO
The aim of this study was to determine the epidemiology (location, microbial load, microbiome, presence/absence of biofilm and pathogens, including ESKAPE-Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter species, and antimicrobial susceptibility profiles) of the bacterial contamination on intensive care units (ICUs) surfaces. Fifty-seven high-touched surfaces were collected from adult, paediatric and neonatal ICUs from two large public Brazilian hospitals from central and north regions. Samples (c. 4 cm2 ) were subjected to culture (qualitative), qPCR targeting 16s rRNA gene (microbial load-bacteria per cm2 ), 16s rRNA amplicon sequencing (microbiome analysis) and scanning electron (SEM) or confocal laser scanning microscopy (CLSM) (biofilm presence). Multidrug resistant organisms (MROs) were detected using specific chromogenic agar. The average bacterial load was 1·32 × 104 bacteria per cm2 , container for newborn feeding bottles, stretcher mattress, humidicrib mattress filling and computer keyboards presented the higher bioburden. However, only 45·6% (26/57) were culture-positive, including 4/26 with MROs. ESKAPE organisms were detected in 51·8% of the samples subjected to next-generation sequencing. Viability staining and CLSM demonstrated live bacteria on 76·7% of culture-negative samples. Biofilm was present on all surfaces subjected to microscopy (n = 56), demonstrating that current cleaning practices are suboptimal and reinforcing that MROs are incorporated into hospital surfaces biofilm. SIGNIFICANCE AND IMPACT OF THE STUDY: Contamination of healthcare facilities surfaces has been shown to play a major role in transmission of pathogens. The findings of this study show that dry surface biofilms are widespread and can incorporate pathogens and multidrug-resistant organisms (MROs). Biofilms on highly touched surfaces pose a risk to patients, as dry surface biofilms persist for long period and micro-organisms within biofilm have been shown to be transmitted. This study also provides a better understanding of microbial populations in hospital environments, reinforcing that pathogens and MROs are found incorporated into biofilm, which impacts the difficulty in cleaning/disinfection.
Assuntos
Biofilmes/crescimento & desenvolvimento , Desinfecção/métodos , Contaminação de Equipamentos/estatística & dados numéricos , Unidades de Terapia Intensiva , Acinetobacter baumannii/crescimento & desenvolvimento , Acinetobacter baumannii/isolamento & purificação , Brasil , Infecção Hospitalar/prevenção & controle , Farmacorresistência Bacteriana Múltipla , Enterococcus faecium/crescimento & desenvolvimento , Enterococcus faecium/isolamento & purificação , Contaminação de Equipamentos/prevenção & controle , Humanos , Recém-Nascido , Klebsiella pneumoniae/crescimento & desenvolvimento , Klebsiella pneumoniae/isolamento & purificação , Microbiota , Microscopia Confocal , Microscopia Eletrônica de Varredura , Pseudomonas aeruginosa/crescimento & desenvolvimento , Pseudomonas aeruginosa/isolamento & purificação , RNA Ribossômico 16S , Staphylococcus aureus/crescimento & desenvolvimento , Staphylococcus aureus/isolamento & purificaçãoRESUMO
BACKGROUND: During functionality testing and packaging of reusable surgical instruments (RSI) for sterilization, instruments are frequently touched. There is a lack of standards relating to hand hygiene frequency and use of gloves in the sterilizing service unit packing area. AIM: To determine the effect of hand hygiene and glove use on maintenance of RSI cleanliness. METHODS: Following manual and automated cleaning, Halsted-mosquito forceps were assessed for adenosine triphosphate (ATP), protein and microbial contamination after handling with gloved and ungloved but washed hands using an ATP surface swab test, bicinchoninic acid assay, and standard culture plate/broth, respectively. Gram's stain was used to classify the isolates. RSI contamination was assessed immediately following and 1, 2, and 4 h after washing hands. FINDINGS: Packing instruments with hands that had been unwashed for 2 or 4 h resulted in a significant increase in contaminating ATP when compared with all other treatment groups (P < 0.05). There was a significant correlation between the time since washing hands, the amount of ATP (r = 0.93; P ≤ 0.001), and the microbial load (r = 0.83; P ≤ 0.001) contaminating the forceps, where the longer the time the hands remained unwashed the higher the contamination. Significantly more contaminating protein was found on forceps handled with ungloved hands that had not been washed for 2 or 4 h (P < 0.001). CONCLUSION: Critical RSI inspection, assembling, lubricating and packing should be performed using either gloves or within 1 h of washing hands.