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1.
Biol Res ; 51(1): 58, 2018 Dec 29.
Artigo em Inglês | MEDLINE | ID: mdl-30594239

RESUMO

BACKGROUND: Studies have demonstrated that transforming growth factor beta-1 (TGF-ß1) exhibits oncogenic activity in different types of cancer, including ovarian cancer (OC). However, its regulatory mechanism in OC and whether TGF-ß1 is involved in chemosensitivity regulation remains unclear. Thus, the aim of this study was to investigate the role of TGF-ß1 in OC. METHODS: The OC cell line SKOV3 was employed, and TGF-ß1 overexpression or knockdown vectors were constructed. The cell proliferation of SKOV3 was evaluated with the cell counting kit (CCK8) kit after treatment with different concentrations of cis-platinum. Western blot and protein immunoprecipitation were employed to detect changes in BRCA1 and Smad3 expression and their interactions. Tumor growth in nude mice was evaluated. RESULTS: The results showed that TGF-ß1 knockdown increased chemosensitivity by promoting BRCA1 expression and Smad3 phosphorylation. In vivo studies showed that TGF-ß1 knockdown significantly inhibited the growth of tumors, also by upregulating BRCA1 expression and Smad3 phosphorylation. CONCLUSION: Taken together, our results suggest that TGF-ß1 knockdown inhibits tumor growth and increases chemosensitivity by promotion of BRCA1/Smad3 signaling.


Assuntos
Regulação para Baixo/fisiologia , Genes BRCA1/fisiologia , Neoplasias Ovarianas/metabolismo , Proteína Smad3/fisiologia , Fator de Crescimento Transformador beta1/fisiologia , Animais , Western Blotting , Linhagem Celular Tumoral , Proliferação de Células , Células Cultivadas , Resistencia a Medicamentos Antineoplásicos/fisiologia , Feminino , Técnicas de Silenciamento de Genes , Humanos , Imuno-Histoquímica , Masculino , Camundongos Endogâmicos BALB C , Neoplasias Ovarianas/tratamento farmacológico , Neoplasias Ovarianas/patologia , Reação em Cadeia da Polimerase em Tempo Real , Proteína Smad3/análise , Fator de Crescimento Transformador beta1/análise , Proteínas Supressoras de Tumor/fisiologia
2.
Biol. Res ; 51: 58, 2018. graf
Artigo em Inglês | LILACS | ID: biblio-1011402

RESUMO

BACKGROUND: Studies have demonstrated that transforming growth factor beta-1 (TGF-ß1) exhibits oncogenic activity in different types of cancer, including ovarian cancer (OC). However, its regulatory mechanism in OC and whether TGF-ß1 is involved in chemosensitivity regulation remains unclear. Thus, the aim of this study was to investigate the role of TGF-ß1 in OC. METHODS: The OC cell line SKOV3 was employed, and TGF-ß1 overexpression or knockdown vectors were constructed. The cell proliferation of SKOV3 was evaluated with the cell counting kit (CCK8) kit after treatment with different concentrations of cis-platinum. Western blot and protein immunoprecipitation were employed to detect changes in BRCA1 and Smad3 expression and their interactions. Tumor growth in nude mice was evaluated. RESULTS: The results showed that TGF-ß1 knockdown increased chemosensitivity by promoting BRCA1 expression and Smad3 phosphorylation. In vivo studies showed that TGF-ß1 knockdown significantly inhibited the growth of tumors, also by upregulating BRCA1 expression and Smad3 phosphorylation. CONCLUSION: Taken together, our results suggest that TGF-ß1 knockdown inhibits tumor growth and increases chemosensitivity by promotion of BRCA1/Smad3 signaling.


Assuntos
Humanos , Animais , Masculino , Feminino , Neoplasias Ovarianas/metabolismo , Regulação para Baixo/fisiologia , Genes BRCA1/fisiologia , Proteína Smad3/fisiologia , Fator de Crescimento Transformador beta1/fisiologia , Neoplasias Ovarianas/patologia , Neoplasias Ovarianas/tratamento farmacológico , Imuno-Histoquímica , Células Cultivadas , Western Blotting , Resistencia a Medicamentos Antineoplásicos/fisiologia , Proteínas Supressoras de Tumor/fisiologia , Linhagem Celular Tumoral , Proliferação de Células , Proteína Smad3/análise , Fator de Crescimento Transformador beta1/análise , Técnicas de Silenciamento de Genes , Reação em Cadeia da Polimerase em Tempo Real , Camundongos Endogâmicos BALB C
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