RESUMO
The dwarf and narrow-leaf rice (Oryza sativa L.) mutant dnl3 was isolated from the Japonica cultivar Zhonghua 11 (wild-type). dnl3 exhibited pleiotropic developmental defects. The narrow-leaf phenotype resulted from a marked reduction in the number of vascular bundles, while the dwarf stature was caused by the formation of foreshortened internodes and a reduced number of parenchyma cells. The suggestion that cell division is impaired in the mutant was consistent with the transcriptional behavior of various genes associated with cell division. The mutant was less responsive to exogenously supplied gibberellic acid than the wild-type, and profiling the transcription of genes involved in gibberellin synthesis and response revealed that a lesion in the mutant affected gibberellin signal transduction. The dnl3 phenotype was inherited as a single-dominant gene, mapping within a 19.1-kb region of chromosome 12, which was found to harbor three open reading frames. Resequencing the open reading frames revealed that the mutant carried an allele at one of the three genes that differed from the wild-type sequence by 2-bp deletions; this gene encoded a cellulose synthase-like D4 (CSLD4) protein. Therefore, OsCSLD4 is a candidate gene for DNL3. DNL3 was expressed in all of the rice organs tested at the heading stage, particularly in the leaves, roots, and culms. These results suggest that DNL3 plays important roles in rice leaf morphogenesis and vegetative development.
Assuntos
Oryza/genética , Filogenia , Folhas de Planta/genética , Proteínas de Plantas/genética , Sequência de Aminoácidos/genética , Divisão Celular/genética , Mapeamento Cromossômico , Clonagem Molecular , Regulação da Expressão Gênica de Plantas , Genótipo , Proteínas Mutantes/biossíntese , Proteínas Mutantes/genética , Oryza/crescimento & desenvolvimento , Fenótipo , Folhas de Planta/crescimento & desenvolvimento , Proteínas de Plantas/biossínteseRESUMO
The aim of this study was to investigate the expression of CD44 and its clinical significance in children suffering from hepatoblastoma (HB). CD44 expression was detected with immunohistochemistry staining in 30 samples from hepatoblastoma children and 10 normal liver tissue samples from normal children. The data obtained was statistically analyzed using the chi-square test, using the SPSS (v.11.0) software. The rate of CD44 expression was significantly higher (66.7%) in hepatoblastoma tissues than in normal liver tissues (χ(2) = 4.848, P < 0.05). The rate of CD44 expression was significantly higher in children with stage III or IV hepatoblastoma (83.3%) than that in children with stage I and II hepatoblastoma (χ(2) ï¼ 5.625, P < 0.05) (41.7%). Therefore, CD44 expression might play an important role in the pathogenesis, progression, and prognosis of HB in children.
Assuntos
Hepatoblastoma/metabolismo , Hepatoblastoma/patologia , Receptores de Hialuronatos/metabolismo , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patologia , Criança , Progressão da Doença , Feminino , Expressão Gênica , Hepatoblastoma/genética , Humanos , Receptores de Hialuronatos/genética , Imuno-Histoquímica , Neoplasias Hepáticas/genética , Masculino , Estadiamento de Neoplasias , PrognósticoRESUMO
We investigated the biological significance of microRNA-126 (miR-126) expression in patients with atrial fibrillation (AF) and/or heart failure (HF) to examine the possible mechanism of miR-126-dependent AF and development of HF. A total of 103 patients were divided into three groups: AF group (18 men and 17 women, mean age: 65.62±12.72 years), HF group (17 men and 15 women, mean age: 63.95±19.71 years), and HF-AF group (20 men and 16 women, mean age: 66.56±14.37 years). Quantitative real-time PCR was used to measure relative miR-126 expression as calculated by the 2−ΔΔCt method. miR-126 was frequently downregulated in the 3 patient groups compared with controls. This reduction was significantly lower in permanent and persistent AF patients than in those with paroxysmal AF (P<0.05, t-test). Moreover, miR-126 expression was markedly lower in the HF-AF group compared with the AF and HF groups. The 3 patient groups had higher N-terminal prohormone brain natriuretic peptide (NT-proBNP) levels, lower left ventricular ejection fraction (LVEF), larger left atrial diameter, and higher cardiothoracic ratio compared with controls. There were significant differences in NT-proBNP levels and LVEF among the AF, HF, and HF-AF groups. Pearson correlation analysis showed that relative miR-126 expression was positively associated with LVEF, logarithm of NT-proBNP, left atrial diameter, cardiothoracic ratio, and age in HF-AF patients. Multiple linear regression analysis showed that miR-126 expression was positively correlated with LVEF, but negatively correlated with the logarithm of NT-pro BNP and the cardiothoracic ratio (all P<0.05). Serum miR-126 levels could serve as a potential candidate biomarker for evaluating the severity of AF and HF. However, to confirm these results, future studies with a larger and diverse patient population are necessary.
Assuntos
Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fibrilação Atrial/metabolismo , Insuficiência Cardíaca/metabolismo , MicroRNAs/metabolismo , Fibrilação Atrial/diagnóstico , Função Atrial/fisiologia , Biomarcadores/metabolismo , Insuficiência Cardíaca/diagnóstico , Modelos Lineares , Peptídeo Natriurético Encefálico/sangue , Prognóstico , Fragmentos de Peptídeos/sangue , Reação em Cadeia da Polimerase em Tempo Real , Função Ventricular Esquerda/fisiologiaRESUMO
We investigated the biological significance of microRNA-126 (miR-126) expression in patients with atrial fibrillation (AF) and/or heart failure (HF) to examine the possible mechanism of miR-126-dependent AF and development of HF. A total of 103 patients were divided into three groups: AF group (18 men and 17 women, mean age: 65.62±12.72 years), HF group (17 men and 15 women, mean age: 63.95±19.71 years), and HF-AF group (20 men and 16 women, mean age: 66.56±14.37 years). Quantitative real-time PCR was used to measure relative miR-126 expression as calculated by the 2-ΔΔCt method. miR-126 was frequently downregulated in the 3 patient groups compared with controls. This reduction was significantly lower in permanent and persistent AF patients than in those with paroxysmal AF (P<0.05, t-test). Moreover, miR-126 expression was markedly lower in the HF-AF group compared with the AF and HF groups. The 3 patient groups had higher N-terminal prohormone brain natriuretic peptide (NT-proBNP) levels, lower left ventricular ejection fraction (LVEF), larger left atrial diameter, and higher cardiothoracic ratio compared with controls. There were significant differences in NT-proBNP levels and LVEF among the AF, HF, and HF-AF groups. Pearson correlation analysis showed that relative miR-126 expression was positively associated with LVEF, logarithm of NT-proBNP, left atrial diameter, cardiothoracic ratio, and age in HF-AF patients. Multiple linear regression analysis showed that miR-126 expression was positively correlated with LVEF, but negatively correlated with the logarithm of NT-pro BNP and the cardiothoracic ratio (all P<0.05). Serum miR-126 levels could serve as a potential candidate biomarker for evaluating the severity of AF and HF. However, to confirm these results, future studies with a larger and diverse patient population are necessary.
Assuntos
Fibrilação Atrial/metabolismo , Insuficiência Cardíaca/metabolismo , MicroRNAs/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , Fibrilação Atrial/diagnóstico , Função Atrial/fisiologia , Biomarcadores/metabolismo , Feminino , Insuficiência Cardíaca/diagnóstico , Humanos , Modelos Lineares , Masculino , Pessoa de Meia-Idade , Peptídeo Natriurético Encefálico/sangue , Fragmentos de Peptídeos/sangue , Prognóstico , Reação em Cadeia da Polimerase em Tempo Real , Função Ventricular Esquerda/fisiologiaRESUMO
Primary osteoporosis is a common health problem in postmenopausal women. This study aimed to detect the association of the g.19074G>A genetic variant in the osteoprotegerin gene (OPG) with bone mineral density (BMD) and primary osteoporosis. The created restriction site-polymerase chain reaction method was used to investigate the g.19074G>A genetic variant. The BMD of the femoral neck hip, lumbar spine (L2-4), and total hip were assessed by dual-energy X-ray absorptiometry (DEXA) in 856 unrelated Chinese postmenopausal women. We found significant differences in the BMDs of the femoral neck hip, lumbar spine (L2-4), and total hip among different genotypes; individuals with the GG genotype had significantly higher BMDs than those with the GA and AA genotypes (P < 0.05). Our results indicated that the A allele was an increased risk factor for primary osteoporosis and the g.19074G>A genetic variant of the OPG gene was associated with BMD and primary osteoporosis in Chinese postmenopausal women.
Assuntos
Densidade Óssea/genética , Predisposição Genética para Doença/genética , Osteoporose Pós-Menopausa/genética , Osteoprotegerina/genética , Polimorfismo de Nucleotídeo Único , Absorciometria de Fóton , Idoso , Alelos , Povo Asiático/genética , Sítios de Ligação/genética , China , Análise Mutacional de DNA/métodos , Desoxirribonucleases de Sítio Específico do Tipo II/metabolismo , Feminino , Frequência do Gene , Predisposição Genética para Doença/etnologia , Genótipo , Humanos , Desequilíbrio de Ligação , Pessoa de Meia-Idade , Osteoporose Pós-Menopausa/etnologia , Reação em Cadeia da Polimerase , Fatores de RiscoRESUMO
Plant height is one of the most important agronomic traits of rice (Oryza sativa). Dwarf mutants are ideal materials for research on the mechanisms of regulation of rice plant height. We examined a new dwarf and narrow-leaf mutant dnl1. Phenotypic analysis showed that the dnl1 mutant has a thinner culm and more tillers, but the number of grains per panicle, the seed setting rate and the grain weight of dnl1 mutant were found to be significantly lower than in the wild-type. Based on scanning electron microscopic observations, the number of cells in the y-axis in internodes was significantly lower than in the wild-type. In phytohormone induction experiments, dnl1 was gibberellic acid-insensitive. The expression of some genes involved in the gibberellins metabolic pathways was affected in the dnl1 mutant, based on the real-time PCR analysis, suggesting that the dnl1 gene likely plays a role in gibberellin metabolic pathways. Genetic analysis showed that the dwarf and narrow leaf phenotype is controlled by a novel single recessive gene, here referred to as the dwarf and narrow leaf 1 (dnl1), which is located within the region between markers Ind12-11 and RM8214 on the short arm of chromosome 12. By means of fine-mapping strategy, the dnl1 gene was localized within an interval of 285.75 kb physical distance. These results will be useful for dnl1 gene cloning and to improve our understanding of the molecular mechanisms involved in the regulation of growth and development of rice.