RESUMO
A case of intoxication in cattle in Paraguay from seed of the palo santo tree is discussed. Visible toxic signs and lesions observed at necropsy are described. The seed contained 3.5 per cent or more crude saponin. Laboratory studies established that aqueous extracts and crude saponin from the seeds were toxic to one-week-old chicks and adult hamsters.
Assuntos
Doenças dos Bovinos/etiologia , Intoxicação por Plantas/veterinária , Plantas Tóxicas , Saponinas/toxicidade , Animais , Bovinos , Galinhas , Cricetinae , Feminino , Masculino , Mesocricetus , Paraguai , Intoxicação por Plantas/etiologia , Plantas Tóxicas/análise , Saponinas/isolamento & purificação , Sementes/análiseRESUMO
An arbovirus serologic survey of school children on the Northern Leeward Islands (Saba, St. Eustatius and St. Maarten) using haemagglutination inhibition (HI) detected a high proportion of reactors to dengue types 1,2, and 3. Analysis in relation to age showed an increasing proportion to be positive with increasing age. The results of an all-age survey on St. Eustatius 1970 led to the hypothesis of possible dengue serotype 1 activity on St. Eustatius around 1900, whilst it is thought that dengue type 2 was endemic at other times. In view of the continuous high endemic dengue activity the (re)-appearance of dengue serotype 1 next to type 2 and 3 may be the prelude to the occurrence of the dengue shock syndrome.
Assuntos
Anticorpos Antivirais/análise , Vírus da Dengue/imunologia , Adolescente , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Antilhas Holandesas , SorotipagemRESUMO
During outbreaks of dengue fever in the Caribbean in 1977 and 1978 a continuous cell line derived from Aedes pseudoscutellaris was successfully used for the isolation of dengue virus strains from acute human sera. 238 strains were isolated and culture fluid was successfully used as antigen for the identification of several strains. The isolates all produced a marked syncytial cytopathic effect often visible as early as four days after inoculation. The method was successfully employed in the field where, because of their low optimal incubation temperature, the cells suffered no ill effects under ambient conditions. The isolation method was found to be much more sensitive than conventional mouse inoculation.
Assuntos
Vírus da Dengue/isolamento & purificação , Dengue/microbiologia , Aedes/microbiologia , Animais , Sangue/microbiologia , Linhagem Celular , Humanos , Métodos , Índias OcidentaisRESUMO
During outbreaks of dengue fever in the Caribbean in 1977 and 1978 a continuous cell line derived from Aedes pseudoscutellaris was successfully used for the isolation of dengue virus strains from acute human sera, 238 strains were isolated and culture fluid was successfully used as antigen for the identification of several strains. The isolates all produced a marked syncytial cytopathic effect often visible as early as four days after inoculation. The method was successfully employed in the field where, because of their low optimal incubation temperature, the cells suffered no ill effects under ambient conditions. The isolation method was found to be much more sensitive than conventional mouse inoculation (AU)
Assuntos
Humanos , 21003 , Dengue/microbiologia , Vírus da Dengue/isolamento & purificação , Aedes/microbiologia , Sangue/microbiologia , Linhagem Celular , Métodos , Índias OcidentaisAssuntos
Hepatite B/epidemiologia , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Feminino , Hepatite B/diagnóstico , Humanos , Lactente , Recém-Nascido , Pessoa de Meia-Idade , Testes Sorológicos , Trinidad e TobagoRESUMO
Sera from various categories of persons in Port-of-Spain, Trinidad, were examined for the prevalence of antigens and antibodies to the Hepatitis B virus. The results of this preliminary report indicate a low carrier state of the Hepatitis B virus in the Trinidadian population (AU)
Assuntos
Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Recém-Nascido , Pessoa de Meia-Idade , Hepatite B/epidemiologia , Hepatite B/diagnóstico , Testes Sorológicos , Trinidad e TobagoRESUMO
Sera obtained principally from children on the Caribbean islands of Aruba and Curaçao were tested for antibodies to various arboviruses. The test indicated that dengue 2 was endemic on Curaçao but not on Aruba, perhaps because of more effective vector control measures on Aruba. Neutralization tests performed with a samll number of sera suggest that dengue infections may have afforded some level of protection against yellow fever to a portion of the Curacao population.