RESUMO
Two strains of Nocardia spp. were isolated from bovine milk of two individual bulk tank. Molecular identification classified the strains as Nocardia farcinica and Nocardia cyriacigeorgica. The thermorresistance to boiling of the isolates was carried out and was observed bacterial growth after boiling. Our findings indicate the potential risk of pathogen transmission to humans through contaminated milk with Nocardia spp.
Assuntos
Bovinos , Crescimento Bacteriano , Substitutos do Leite Humano , Conservação de Alimentos , Mastite Bovina , Nocardia/isolamento & purificação , Streptococcus bovis/isolamento & purificação , Bovinos , Amostras de Alimentos , Métodos , Leite , VirulênciaRESUMO
Two strains of Nocardia spp. were isolated from bovine milk of two individual bulk tank. Molecular identification classified the strains as Nocardia farcinica and Nocardia cyriacigeorgica. The thermorresistance to boiling of the isolates was carried out and was observed bacterial growth after boiling. Our findings indicate the potential risk of pathogen transmission to humans through contaminated milk with Nocardia spp.
RESUMO
Two strains of Nocardia spp. were isolated from bovine milk of two individual bulk tank. Molecular identification classified the strains as Nocardia farcinica and Nocardia cyriacigeorgica. The thermorresistance to boiling of the isolates was carried out and was observed bacterial growth after boiling. Our findings indicate the potential risk of pathogen transmission to humans through contaminated milk with Nocardia spp.
Assuntos
Aminoglicosídeos , Antibacterianos/uso terapêutico , Doxorrubicina/uso terapêutico , Leucemia P388/tratamento farmacológico , Células Tumorais Cultivadas/efeitos dos fármacos , Animais , Antibacterianos/isolamento & purificação , Células HeLa/efeitos dos fármacos , Humanos , Leucemia L1210/tratamento farmacológico , CamundongosRESUMO
A novel tricyclic diterpenoid antibiotic, brasilicardin A, was isolated from the culture broth of Nocardia brasiliensis IFM 0406. The antibiotic exhibited immunosuppressive activity in a mouse mixed lymphocyte reaction (MLR) assay system and its IC50 value was 0.057 microg/ml. Although the inhibitory activity of cyclosporin A (CyA) against IL-2 production was confirmed in the MLR assay system, brasilicardin A did not have the activity. The results of in vitro toxicity testing of brasilicardin A against various human cell lines were compared with those of CyA.
Assuntos
Aminoglicosídeos , Antibacterianos/farmacologia , Imunossupressores/farmacologia , Nocardia/metabolismo , Animais , Antibacterianos/biossíntese , Antibacterianos/isolamento & purificação , Bactérias/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Cromatografia Líquida de Alta Pressão , Fermentação , Fungos/efeitos dos fármacos , Humanos , Imunofilinas/antagonistas & inibidores , Imunossupressores/isolamento & purificação , Interleucina-2/metabolismo , Teste de Cultura Mista de Linfócitos , Camundongos , Testes de Sensibilidade MicrobianaAssuntos
Alcaloides/isolamento & purificação , Antineoplásicos/isolamento & purificação , Indóis/isolamento & purificação , Nitrilas/química , Nocardia/química , Alcaloides/química , Alcaloides/farmacologia , Antineoplásicos/química , Antineoplásicos/farmacologia , Indóis/química , Indóis/farmacologia , Análise EspectralRESUMO
New benz[alpha]anthraquinone antibiotics (brasiliquinones A, B and C) with an ethyl group at C-3 were isolated. The producer was identified as Nocardia brasiliensis. The antibiotics were active against Gram-positive bacteria including Mycobacterium sp., but not active against Gram-negative bacteria or fungi. They were also active against multiple drug-resistant P388/ADR tumor cells.
Assuntos
Antibacterianos/isolamento & purificação , Nocardia/metabolismo , Animais , Antraquinonas/isolamento & purificação , Antibacterianos/química , Antibacterianos/farmacologia , Fermentação , Camundongos , Nocardia/classificação , Células Tumorais CultivadasRESUMO
A new 32-membered macrolide antibiotic, brasilinolide A was isolated from the fermentation broth of Nocardia sp. IFM 0406. The producer was identified as Nocardia brasiliensis. The antibiotic was only active against Aspergillus niger, but not active against other fungi including yeasts as well as other filamentous like fungi and bacteria. Brasilinolide A exerted an immunosuppressive activity in the assay system of a mixed lymphocyte reaction (MLR).