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ABSTRACT Introduction: The strength training of superior members currently practiced in the university courses does not correspond to the real necessities of the athletes, demanding the development of optimized methods for more expressive performance gains. To create these methods, it is necessary to collect data scientifically to consolidate a solid analysis for improvement. Objective: Analyze upper limbs strength training in table tennis players. Methods: During the experiment, 20 students were randomly divided into groups to perform daily training according to the original table tennis training plan. The experimental group received a schematized protocol improving the existing teaching by adding upper limb strength training. The experiment totaled eight weeks, with one hour of training conducted twice a week. Results: In the experimental group, the attack distance index increased by 25.378%, the one-minute attack swing index increased by 0.585%, the swing index increased by 12.795%, and the technical attack index increased by 11.452%. Conclusion: The improved upper limb strength training method presented in the protocol of this article can optimize athletes' muscle strength, increasing balance and swing ability, positively influencing the technical score and final sports performance. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.
RESUMO Introdução: O treinamento de força de membros superiores atualmente praticados nos cursos universitários não correspondem às reais necessidades dos atletas, exigindo o desenvolvimento de métodos otimizados para ganhos de desempenho mais expressivos. Para a confecção desses métodos, é necessário efetuar-se uma coleta de dados de maneira científica afim de consolidar uma análise sólida para aprimoramento. Objetivo: Analisar o treinamento de força dos membros superiores nos praticantes de tênis de mesa. Métodos: Durante o experimento, 20 estudantes foram aleatoriamente divididos em um grupo para efetuar o treinamento diário de acordo com o plano original de treinamento do tênis de mesa, enquanto o grupo experimental recebeu um protocolo esquematizado aprimorando o ensino existente, adicionando o treinamento de força para os membros superiores. O experimento totalizou 8 semanas, com uma hora de treino realizado duas vezes por semana. Resultados: No grupo experimental, o índice de distância de ataque aumentou 25,378%, o índice de balanço de ataque de um minuto aumentou 0,585%, o índice de balanço aumentou 12,795% e o índice técnico de ataque aumentou 11,452%. Conclusão: O método aperfeiçoado de treinamento de força dos membros superiores apresentados no protocolo deste artigo pode otimizar a força muscular dos atletas, aumentando a capacidade de equilíbrio e balanço, influenciando positivamente na pontuação técnica e desempenho esportivo final. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.
RESUMEN Introducción: El entrenamiento de fuerza de miembros superiores actualmente practicado en los cursos universitarios no corresponde a las necesidades reales de los atletas, exigiendo el desarrollo de métodos optimizados para ganancias de rendimiento más expresivas. Para la confección de estos métodos, es necesario recopilar datos de forma científica con el fin de consolidar un análisis sólido para su mejora. Objetivo: Analizar el entrenamiento de fuerza de los miembros superiores en practicantes de tenis de mesa. Métodos: Durante el experimento, 20 estudiantes se dividieron aleatoriamente en un grupo para realizar un entrenamiento diario según el plan de entrenamiento original de tenis de mesa, mientras que el grupo experimental recibió un protocolo esquematizado que mejoraba la enseñanza existente añadiendo un entrenamiento de fuerza de las extremidades superiores. El experimento duró 8 semanas, con una hora de entrenamiento dos veces por semana. Resultados: En el grupo experimental, el índice de distancia de ataque aumentó un 25,378%, el índice de balanceo de ataque de un minuto aumentó un 0,585%, el índice de balanceo aumentó un 12,795% y el índice de ataque técnico aumentó un 11,452%. Conclusión: El método mejorado de entrenamiento de la fuerza de las extremidades superiores presentado en el protocolo de este artículo puede optimizar la fuerza muscular de los atletas, aumentando el equilibrio y la capacidad de balanceo, influyendo positivamente en la puntuación técnica y en el rendimiento deportivo final. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.
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Oceanic heat anomalies affect climate in remote regions through the atmospheric cycle. South America (SA) was the first region found associated with EI Niño, which affects the fishery, agriculture, forestry, and livestock industry of SA. As approximately 60% of the total water is used for agriculture, climate changes in SA caused by ocean anomalies have led to the variability of available water, especially for irrigation water. Where the precipitation is low and/or the temperature is high, the availability and quality of water resources are under pressure. For instance, droughts associated with La Niña severely limited water supply and irrigation requirements between 25°S - 40°S in west-central Argentina and central Chile. In order to study the relationship between ocean variability and the climate of SA, 19 teleconnection indices (TI) related to Ocean abnormity are considered. The 19 indices are: the sea surface temperature (SST) and their anomaly in 4 Niño regions (SST1 + 2, SST3, SST3.4, SST4, ANOM1 + 2, ANOM3, ANOM3.4, ANOM4), Southern Oscillation Index (SOI), Oceanic Niño Index (ONI), Outgoing Longwave Radiation (OLR), Arctic Oscillation (AO), North Atlantic Oscillation (NAO), Pacific Decadal Oscillation (PDO), Pacific-North America (PNA), Atlantic Multi-decadal Oscillation (AMO), West and East of Indian Ocean Dipole (IODW, IODE), and the difference between IODW and IODE (IODd). High-resolution gridded climate data (1982-2016) from the Global Precipitation Climatology Centre (GPCC), the Climate Prediction Center (CPC), and the National Centers for Environmental Prediction (NCEP) are applied for correlation analyses. The results show that the 89.4% area of South American climate has a significant correlation with the SST in Niño region 1 + 2, the mean correlation coefficient is 0.55 for NCEP precipitation and 0.54 for CPC temperature. The lag duration for the remote correlation is around 2-3 months. It is the first attempt to analyze the correlation relationship based on 19 TIs, which can provide comprehensive insight into the climate of SA at a high-resolution scale. These findings are helpful for identifying the sensitive factors that affect climate in SA, for projecting the climate variables of SA, and for managing the irrigation water resources of SA.
Assuntos
Mudança Climática , Temperatura Alta , América do Sul , TemperaturaRESUMO
Salmonella spp. is one of the most common foodborne infectious pathogen. This study aimed to develop a real-time nucleic acid sequence-based amplification (NASBA) assay for detecting Salmonella in foods. Primers and a molecular beacon targeting the Salmonella-specific xcd gene were designed for mRNA transcription, and 48 Salmonella and 18 non-Salmonella strains were examined. The assay showed a high specificity and low detection limit for Salmonella (7 × 10-1 CFU/mL) after 12 h of pre-enrichment. Importantly, it could detect viable cells. Additionally, the efficacy of the NASBA assay was examined in the presence of pork background microbiota; it could detect Salmonella cells at 9.5 × 103 CFU/mL. Lastly, it was successfully used to detect Salmonella in pork, beef, and milk, and its detection limit was as low as 10 CFU/25 g (mL). The real-time NASBA assay developed in this study may be useful for rapid, specific, and sensitive detection of Salmonella in food of animal origin.
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Carne/microbiologia , Leite/microbiologia , Salmonella/isolamento & purificação , Replicação de Sequência Autossustentável/métodos , Animais , Bovinos , Microbiologia de Alimentos , Salmonella/classificação , Salmonella/genética , SuínosRESUMO
In the previous study, we used genome shuffling to improve fengycin production of the original strain Bacillus amyloliquefaciens ES-2-4. After two rounds of genome shuffling, a high-yield recombinant FMB72 strain that exhibited 8.30-fold increase in fengycin production was obtained. In this study, comparative proteomic analysis of the parental ES-2-4 and genome-shuffled FMB72 strains was conducted to examine the differentially expressed proteins. In the shuffled strain FMB72, 50 differently expressed spots (p<0.05) were selected to be excised and analyzed using Matrix-Assisted Laser Desorption/Ionization Time of Flight/Time of Flight Mass Spectrometry, and finally 44 protein spots were confidently identified according to NCBI database. According to clusters of orthologous groups (COG) functional category analysis and related references, the differentially expressed proteins could be classified into several functional categories, including proteins involved in metabolism, energy generation and conversion, DNA replication, transcription, translation, ribosomal structure and biogenesis, cell motility and secretion, signal transduction mechanisms, general function prediction. Of the 44 identified proteins, signaling proteins ComA and Spo0A may positively regulate fengycin synthesis at transcriptional level. Taken together, the present study will be informative for exploring the exact roles of ComA and Spo0A in fengycin synthesis and explaining the molecular mechanism of fengycin synthesis.
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Bacillus amyloliquefaciens/genética , Bacillus amyloliquefaciens/metabolismo , Proteínas de Bactérias/metabolismo , Lipopeptídeos/biossíntese , Bacillus amyloliquefaciens/química , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Embaralhamento de DNA , Genoma Bacteriano , Proteômica , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por MatrizRESUMO
Abstract In the previous study, we used genome shuffling to improve fengycin production of the original strain Bacillus amyloliquefaciens ES-24. After two rounds of genome shuffling, a high-yield recombinant FMB72 strain that exhibited 8.30-fold increase in fengycin production was obtained. In this study, comparative proteomic analysis of the parental ES-24 and genome-shuffled FMB72 strains was conducted to examine the differentially expressed proteins. In the shuffled strain FMB72, 50 differently expressed spots (p 0.05) were selected to be excised and analyzed using Matrix-Assisted Laser Desorption/Ionization Time of Flight/Time of Flight Mass Spectrometry, and finally 44 protein spots were confidently identified according to NCBI database. According to clusters of orthologous groups (COG) functional category analysis and related references, the differentially expressed proteins could be classified into several functional categories, including proteins involved in metabolism, energy generation and conversion, DNA replication, transcription, translation, ribosomal structure and biogenesis, cell motility and secretion, signal transduction mechanisms, general function prediction. Of the 44 identified proteins, signaling proteins ComA and Spo0A may positively regulate fengycin synthesis at transcriptional level. Taken together, the present study will be informative for exploring the exact roles of ComA and Spo0A in fengycin synthesis and explaining the molecular mechanism of fengycin synthesis.
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Abstract In the previous study, we used genome shuffling to improve fengycin production of the original strain Bacillus amyloliquefaciens ES-2-4. After two rounds of genome shuffling, a high-yield recombinant FMB72 strain that exhibited 8.30-fold increase in fengycin production was obtained. In this study, comparative proteomic analysis of the parental ES-2-4 and genome-shuffled FMB72 strains was conducted to examine the differentially expressed proteins. In the shuffled strain FMB72, 50 differently expressed spots (p < 0.05) were selected to be excised and analyzed using Matrix-Assisted Laser Desorption/Ionization Time of Flight/Time of Flight Mass Spectrometry, and finally 44 protein spots were confidently identified according to NCBI database. According to clusters of orthologous groups (COG) functional category analysis and related references, the differentially expressed proteins could be classified into several functional categories, including proteins involved in metabolism, energy generation and conversion, DNA replication, transcription, translation, ribosomal structure and biogenesis, cell motility and secretion, signal transduction mechanisms, general function prediction. Of the 44 identified proteins, signaling proteins ComA and Spo0A may positively regulate fengycin synthesis at transcriptional level. Taken together, the present study will be informative for exploring the exact roles of ComA and Spo0A in fengycin synthesis and explaining the molecular mechanism of fengycin synthesis.
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Proteínas de Bactérias/metabolismo , Lipopeptídeos/biossíntese , Bacillus amyloliquefaciens/genética , Bacillus amyloliquefaciens/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/química , Genoma Bacteriano , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Embaralhamento de DNA , Proteômica , Bacillus amyloliquefaciens/químicaRESUMO
In the previous study, we used genome shuffling to improve fengycin production of the original strain Bacillus amyloliquefaciens ES-24. After two rounds of genome shuffling, a high-yield recombinant FMB72 strain that exhibited 8.30-fold increase in fengycin production was obtained. In this study, comparative proteomic analysis of the parental ES-24 and genome-shuffled FMB72 strains was conducted to examine the differentially expressed proteins. In the shuffled strain FMB72, 50 differently expressed spots (p < 0.05) were selected to be excised and analyzed using Matrix-Assisted Laser Desorption/Ionization Time of Flight/Time of Flight Mass Spectrometry, and finally 44 protein spots were confidently identified according to NCBI database. According to clusters of orthologous groups (COG) functional category analysis and related references, the differentially expressed proteins could be classified into several functional categories, including proteins involved in metabolism, energy generation and conversion, DNA replication, transcription, translation, ribosomal structure and biogenesis, cell motility and secretion, signal transduction mechanisms, general function prediction. Of the 44 identified proteins, signaling proteins ComA and Spo0A may positively regulate fengycin synthesis at transcriptional level. Taken together, the present study will be informative for exploring the exact roles of ComA and Spo0A in fengycin synthesis and explaining the molecular mechanism of fengycin synthesis.(AU)
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Purpose: To evaluate the effect of transforming growth factor 1 (TGF-1) on tendon-to-bone reconstruction of rotator cuff tears. Methods: Seventy-two rat supraspinatus tendons were transected and reconstructed in situ. At 8 and 16 weeks, specimens of three groups; that is control, L-dose (low dose), and H-dose (high dose) were harvested and underwent a biomechanical test to evaluate the maximum load and stiffness values. Histology sections of the tendon-to-bone interface were identified by hematoxylin-eosin or Masson trichrome stain. Collagen type III was observed by picric acid sirius red staining under polarized light. The level of insulin-like growth factor 1 (IGF-1) and vascular endothelial growth factor (VEGF) was measured by the enzyme-linked immunosorbent assay (ELISA) method. Results: Collagen type III of the H-dose group had a significant difference in histology structure compared with the L-dose group (P 0.05). The maximum load and stiffness decreased significantly in the control group compared with the values of the L-dose and H-dose groups. The stiffness among the three groups differed significantly at the same postoperative time (P 0.05). Interestingly, progressive reestablishment of collagen type III affected tendon-to-bone healing significantly in the later stages. Conclusion: The H-dose was associated with an increased collagen type III morphology stimulated by TGF-1.(AU)
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Animais , Masculino , Ratos , Lesões do Manguito Rotador/terapia , Fator de Crescimento Transformador beta1/uso terapêutico , Colágeno Tipo III/biossíntese , Modelos Animais , Ratos Sprague-Dawley , Ensaio de Imunoadsorção Enzimática/métodosRESUMO
Abstract Purpose: To evaluate the effect of transforming growth factor β1 (TGF-β1) on tendon-to-bone reconstruction of rotator cuff tears. Methods: Seventy-two rat supraspinatus tendons were transected and reconstructed in situ. At 8 and 16 weeks, specimens of three groups; that is control, L-dose (low dose), and H-dose (high dose) were harvested and underwent a biomechanical test to evaluate the maximum load and stiffness values. Histology sections of the tendon-to-bone interface were identified by hematoxylin-eosin or Masson trichrome stain. Collagen type III was observed by picric acid sirius red staining under polarized light. The level of insulin-like growth factor 1 (IGF-1) and vascular endothelial growth factor (VEGF) was measured by the enzyme-linked immunosorbent assay (ELISA) method. Results: Collagen type III of the H-dose group had a significant difference in histology structure compared with the L-dose group (P<0.05). The maximum load and stiffness decreased significantly in the control group compared with the values of the L-dose and H-dose groups. The stiffness among the three groups differed significantly at the same postoperative time (P<0.05). Interestingly, progressive reestablishment of collagen type III affected tendon-to-bone healing significantly in the later stages. Conclusion: The H-dose was associated with an increased collagen type III morphology stimulated by TGF-β1.
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Animais , Masculino , Ratos , Traumatismos dos Tendões/tratamento farmacológico , Cicatrização/fisiologia , Manguito Rotador/cirurgia , Fatores de Crescimento do Endotélio Vascular/metabolismo , Fator de Crescimento Transformador beta1/metabolismo , Lesões do Manguito Rotador/cirurgia , Traumatismos dos Tendões/metabolismo , Resistência à Tração/fisiologia , Cicatrização/efeitos dos fármacos , Fenômenos Biomecânicos , Ensaio de Imunoadsorção Enzimática , Manguito Rotador/metabolismo , Ratos Sprague-Dawley , Colágeno Tipo III/metabolismo , Modelos Animais de Doenças , Elasticidade/fisiologia , Fator de Crescimento Transformador beta1/farmacologia , Força Muscular/fisiologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/fisiologia , Lesões do Manguito Rotador/metabolismoRESUMO
PURPOSE: To evaluate the effect of transforming growth factor ß1 (TGF-ß1) on tendon-to-bone reconstruction of rotator cuff tears. METHODS: Seventy-two rat supraspinatus tendons were transected and reconstructed in situ. At 8 and 16 weeks, specimens of three groups; that is control, L-dose (low dose), and H-dose (high dose) were harvested and underwent a biomechanical test to evaluate the maximum load and stiffness values. Histology sections of the tendon-to-bone interface were identified by hematoxylin-eosin or Masson trichrome stain. Collagen type III was observed by picric acid sirius red staining under polarized light. The level of insulin-like growth factor 1 (IGF-1) and vascular endothelial growth factor (VEGF) was measured by the enzyme-linked immunosorbent assay (ELISA) method. RESULTS: Collagen type III of the H-dose group had a significant difference in histology structure compared with the L-dose group (P<0.05). The maximum load and stiffness decreased significantly in the control group compared with the values of the L-dose and H-dose groups. The stiffness among the three groups differed significantly at the same postoperative time (P<0.05). Interestingly, progressive reestablishment of collagen type III affected tendon-to-bone healing significantly in the later stages. CONCLUSION: The H-dose was associated with an increased collagen type III morphology stimulated by TGF-ß1.