RESUMO

In Enterobacteriaceae, the blaNDM genes have been found in many different genetic contexts, and a wide diversity of plasmid scaffolds bearing those genes has been found. In August 2013, we identified NDM-1-producing Escherichia coli and Enterobacter hormaechei strains from a single rectal swab sample from a patient hospitalized in Rio de Janeiro, Brazil, who had no history of travel abroad. Complete DNA sequencing using the Illumina platform and annotation of the two plasmids harboring the blaNDM-1 gene, one from each strain, showed that they belonged to incompatibility groups IncFIIK and IncX3 and harbored a novel transposon named Tn3000. Similar genetic structures have been identified among other isolates in Brazil but also on plasmids from other continents. Our findings suggest that the blaNDM-1 gene may be transmitted by Tn3000 in different parts of the world.

Assuntos

Elementos de DNA Transponíveis/genética , Enterobacter/isolamento & purificação , Escherichia coli/isolamento & purificação , beta-Lactamases/metabolismo , Antibacterianos/farmacologia , Aztreonam/farmacologia , Proteínas de Bactérias/genética , Sequência de Bases , Brasil , Conjugação Genética , Sequência Conservada , Enterobacter/efeitos dos fármacos , Enterobacter/genética , Enterobacter/metabolismo , Infecções por Enterobacteriaceae/microbiologia , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Escherichia coli/metabolismo , Fosfomicina/farmacologia , Humanos , Índia , Testes de Sensibilidade Microbiana , Marrocos , Nepal , Plasmídeos , Reto/microbiologia , beta-Lactamases/genética