RESUMO
A major challenge in proteomic biomarker discovery and validation for psychiatric diseases is the inherent biological complexity underlying these conditions. There are also many technical issues which hinder this process such as the lack of standardization in sampling, processing and storage of bio-samples in preclinical and clinical settings. This chapter describes a reproducible procedure for sampling blood serum and plasma that is specifically designed for maximizing data quality output in two-dimensional gel electrophoresis, multiplex immunoassay and mass spectrometry profiling studies.
Assuntos
Biomarcadores/sangue , Coleta de Amostras Sanguíneas/métodos , Transtornos Mentais/sangue , Proteômica/métodos , Coagulação Sanguínea , Preservação de Sangue/métodos , Coleta de Amostras Sanguíneas/instrumentação , Centrifugação , Criopreservação/métodos , Humanos , Prontuários Médicos , Flebotomia/métodos , Plasma , Soro , Manejo de Espécimes/instrumentação , Manejo de Espécimes/métodosRESUMO
A major challenge in single or panel of biomarker discovery and validation is the inherent biological complexity underlying disease heterogeneity and inconsistent responses to treatment. Moreover, the lack of standardization in the sampling, processing, and storage of biological fluids such as plasma and serum disrupts the discovery and validation of blood-based biomarker tests in preclinical and clinical settings. This chapter presents a reproducible sample collection and handling procedure that aims to enhance analyte stability and ensure compatibility with the corresponding multiplex biomarker profiling platforms. The importance of defining bio-sample acquisition and processing, study design, and profiling platform guidelines for blood-based biomarker measurements is paramount for the success of personalized healthcare strategy and development of companion diagnostics.