RESUMO

The present work evaluated the effects of feeding graded levels of whole cottonseed on reproductive parameters of Red Sokoto bucks. Twenty Red Sokoto bucks were used for the experiment. After a 14-day pre-treatment period, bucks were assigned for 90 days to one of four isonitrogeneous treatments: control (diet A); 0 mg kg-1of total gossypol, (diet B); 15% mg kg-1of total gossypol, (diet C); 30% mg kg-1of total gossypol and (diet D); 45% mg kg-1of total gossypol.The mean percentage sperm gross motility was significantly (p < 0.05) lower in group D (45% WCS) compared to groups C (30% WCS) and A (control) at days 60, 75 and 90. The mean semen pH and reaction time were not significantly (p > 0.05)different among treatment groups. Semen colour of the bucks in the control group was majorly creamy, in group C (30% WCS) and B (15% WCS) creamy to milky and group D (45% WCS) colourless. The mean semen volume was significantly (p < 0.05) higher in groupsC and A compared to group D (45% WCS) at days 60, 75 and 90. The mean semen concentration was significantly (p < 0.05) lower in group D (45% WCS) when compared to group A (control) at days 30, 45, 60, 75 and 90. Group A (control) bucks had significantly (p < 0.05) higher percentage live sperm compared to those in group D (45% WCS) at days 45, 60, 75 and 90. Mean Sperm morphological abnormalities including detached head, free tail curved tail and midpiece droplets were significantly (p < 0.05) higher in group D (45%) than in group A (Control) at day 15 (for free tails) and day 90 (for all). Testicular and epididymal sperm reserves were higher in animals supplemented with up to 30% whole cottonseed.In conclusion, feeding bucks above 30% WCS resulted in more deleterious effects on the semen characteristics and testicular profile.(AU)

Assuntos

RESUMO

The present work evaluated the effects of feeding graded levels of whole cottonseed on reproductive parameters of Red Sokoto bucks. Twenty Red Sokoto bucks were used for the experiment. After a 14-day pre-treatment period, bucks were assigned for 90 days to one of four isonitrogeneous treatments: control (diet A); 0 mg kg-1of total gossypol, (diet B); 15% mg kg-1of total gossypol, (diet C); 30% mg kg-1of total gossypol and (diet D); 45% mg kg-1of total gossypol.The mean percentage sperm gross motility was significantly (p 0.05)different among treatment groups. Semen colour of the bucks in the control group was majorly creamy, in group C (30% WCS) and B (15% WCS) creamy to milky and group D (45% WCS) colourless. The mean semen volume was significantly (p < 0.05) higher in groupsC and A compared to group D (45% WCS) at days 60, 75 and 90. The mean semen concentration was significantly (p < 0.05) lower in group D (45% WCS) when compared to group A (control) at days 30, 45, 60, 75 and 90. Group A (control) bucks had significantly (p < 0.05) higher percentage live sperm compared to those in group D (45% WCS) at days 45, 60, 75 and 90. Mean Sperm morphological abnormalities including detached head, free tail curved tail and midpiece droplets were significantly (p < 0.05) higher in group D (45%) than in group A (Control) at day 15 (for free tails) and day 90 (for all). Testicular and epididymal sperm reserves were higher in animals supplemented with up to 30% whole cottonseed.In conclusion, feeding bucks above 30% WCS resulted in more deleterious effects on the semen characteristics and testicular profile.

Assuntos

RESUMO

In male goats, being in permanent visual contact with females in estrus does not prevent seasonal variation in certain endocrine hormone levels and sexual activities. In this study, we tested whether continuous and full contact with females in estrus prevented seasonal endocrinological variation in bucks. In 1 experiment (Exp. 1), we verified that the sudden introduction of goats in estrus increased the plasma concentrations of androgen in bucks during the nonbreeding season under our experimental conditions. In another experiment (Exp. 2), we tested the ability of estrous goats to prevent seasonal inhibition of LH and androgen secretions in bucks kept in permanent and full contact with them. In Exp. 1, 3 groups of bucks (n = 5 in each group) were isolated from females from the months of July to January. On January 27, one group continued being isolated from females; a second group was exposed to ovariectomized, untreated goats; and a third group was exposed to ovariectomized goats with induced estrus. Plasma androgen concentrations were determined every 2 h from 8 h before to 8 h after the introduction of females. The introduction of estrus-induced goats significantly increased androgen concentrations, which were higher than in the isolated bucks, as well as in those exposed to untreated goats (P < 0.05). In Exp. 2 (n = 5 per group), one group of bucks was isolated from females from October to July, whereas two other groups remained in contact with ovariectomized goats, either untreated or regularly induced to estrus. In the three groups of bucks, plasma concentrations of LH were determined once during the months of October, February, March, and June, whereas androgen concentrations were determined weekly from October to July. The mean plasma LH and androgen concentrations were low and did not differ among the groups of bucks during the normal seasonal period of sexual inactivity (P > 0.05). We conclude that full contact and sexual interactions with estrus-induced goats failed to stop the seasonality of LH and androgen plasma concentrations of bucks, although bucks could respond to the introduction of females by acute increases in plasma LH and androgen.

Assuntos

RESUMO

O objetivo do estudo foi avaliar a influência da bipartição escrotal sobre a capacidade de termorregulação do testículo caprino, testando-se a hipótese de que animais com bipartição possuem menor temperatura testicular, maior capacidade hemodinâmica local, maior número de células germinativas e maior produção espermática. Foram selecionados 8 bodes, divididos de acordo com a conformação escrotal: G1 (n=4) animais com até 50% de bipartição; GC (n=4) grupo controle, sem bipartição. Todos foram submetidos à mensuração da temperatura retal, termografia corporal e escrotal. Exames ultrassonográficos Doppler foram conduzidos no plexo pampiniforme. Adicionalmente os animais foram submetidos à punção biópsia aspirativa testicular (PBA) e 2 ejaculados de cada reprodutor foram colhidos para avaliação espermática. Não foram observadas diferenças para os resultados da PBA, da termografia escrotal e do espermograma comparando-se os dois grupos. Na avaliação hemodinâmica apenas o IR (0,53a+-0,11 e 0,46b+- 0,06; p=0,0108) foi superior para os animais bipartidos. Conclui-se que caprinos que possuem até 50% de bipartição escrotal possuem qualidade espermática semelhante a animais não bipartidos, não possuem menor temperatura testicular e maior número de células germinativas primordiais como se supunha na hipótese inicial da pesquisa.

The objective of this study was to evaluate the influence of the bipartition of the scrotum on the thermoregulatory capacity of the goat testicle, testing the hypothesis that bucks with bipartition of the scrotum have minor testicular temperature, greater local hemodynamic capacity, more germ cells and higher sperm production capacity. Eight male goats were used and divided according to the conformation of the scrotum: G1 (n=4) animals with scrotal bipartition below 50%; CG (n=4) control group without scrotal bipartition. All the animals were submitted to the evaluation of rectal temperature and scrotal and body surface thermography. Doppler ultrasonography held at the spermatic cord region was performed. In addition, the animals underwent fine needle aspiration testicular cytology (FNAC) and two ejaculates from each buck were collected. There were no significant differences between G1 and GC for FNAC and for testicular thermography. On the hemodynamics evaluation the IR was higher for the animals with scrotal bipartition than to control group (0.53a+-0.11 and 0.46b+- 0.06; P=0.0108). In conclusion, goats up to 50% of the scrotum bipartition have the same sperm quality than the control group, and the bipartition was not related to more efficiency on the thermoregulation process or number of testicular primordial cells in this study.

El objetivo del estudio fue evaluar la influencia de la bipartición escrotal sobre la capacidad de termorregulación del testículo caprino, probándose la hipótesis de que animales con bipartición tienen menor temperatura testicular, mayor capacidad hemodinámica local, mayor número de células germinativas y mayor producción espermática. Se seleccionaron 8 machos, divididos de acuerdo con la conformación escrotal: G1 (n=4) animales con hasta 50% de bipartición; GC (n=4) grupo de control, sin bipartición. Todos ellos fueron sometidos a la medición de la temperatura rectal, termografía corporal y escrotal. Los exámenes ultrasonográficos Doppler se realizaron en el plexo pampiniforme. Adicionalmente los animales fueron sometidos a la punción biopsia aspirativa testicular (PBA) y 2 eyaculaciones de cada reproductor fueron cosechados para evaluación espermática. No se observaron diferencias para los resultados de la PBA, de la termografía escrotal y del espermograma comparando los dos grupos. En la evaluación hemodinámica sólo el IR (0,53a +- 0,11 y 0,46b +- 0,06, p = 0,0108) fue superior para los animales bipartidos. Se concluye que los caprinos que tieren hasta 50% de bipartición escrotal tienen calidad espermática similares a animales no bipartidos, no tienen menor temperatura testicular y mayor número de células germinativas primordiales como se suponía en la hipótesis inicial de la investigación.

Assuntos

RESUMO

O objetivo do estudo foi avaliar a influência da bipartição escrotal sobre a capacidade de termorregulação do testículo caprino, testando-se a hipótese de que animais com bipartição possuem menor temperatura testicular, maior capacidade hemodinâmica local, maior número de células germinativas e maior produção espermática. Foram selecionados 8 bodes, divididos de acordo com a conformação escrotal: G1 (n=4) animais com até 50% de bipartição; GC (n=4) grupo controle, sem bipartição. Todos foram submetidos à mensuração da temperatura retal, termografia corporal e escrotal. Exames ultrassonográficos Doppler foram conduzidos no plexo pampiniforme. Adicionalmente os animais foram submetidos à punção biópsia aspirativa testicular (PBA) e 2 ejaculados de cada reprodutor foram colhidos para avaliação espermática. Não foram observadas diferenças para os resultados da PBA, da termografia escrotal e do espermograma comparando-se os dois grupos. Na avaliação hemodinâmica apenas o IR (0,53a+-0,11 e 0,46b+- 0,06; p=0,0108) foi superior para os animais bipartidos. Conclui-se que caprinos que possuem até 50% de bipartição escrotal possuem qualidade espermática semelhante a animais não bipartidos, não possuem menor temperatura testicular e maior número de células germinativas primordiais como se supunha na hipótese inicial da pesquisa.(AU)

The objective of this study was to evaluate the influence of the bipartition of the scrotum on the thermoregulatory capacity of the goat testicle, testing the hypothesis that bucks with bipartition of the scrotum have minor testicular temperature, greater local hemodynamic capacity, more germ cells and higher sperm production capacity. Eight male goats were used and divided according to the conformation of the scrotum: G1 (n=4) animals with scrotal bipartition below 50%; CG (n=4) control group without scrotal bipartition. All the animals were submitted to the evaluation of rectal temperature and scrotal and body surface thermography. Doppler ultrasonography held at the spermatic cord region was performed. In addition, the animals underwent fine needle aspiration testicular cytology (FNAC) and two ejaculates from each buck were collected. There were no significant differences between G1 and GC for FNAC and for testicular thermography. On the hemodynamics evaluation the IR was higher for the animals with scrotal bipartition than to control group (0.53a+-0.11 and 0.46b+- 0.06; P=0.0108). In conclusion, goats up to 50% of the scrotum bipartition have the same sperm quality than the control group, and the bipartition was not related to more efficiency on the thermoregulation process or number of testicular primordial cells in this study.(AU)

El objetivo del estudio fue evaluar la influencia de la bipartición escrotal sobre la capacidad de termorregulación del testículo caprino, probándose la hipótesis de que animales con bipartición tienen menor temperatura testicular, mayor capacidad hemodinámica local, mayor número de células germinativas y mayor producción espermática. Se seleccionaron 8 machos, divididos de acuerdo con la conformación escrotal: G1 (n=4) animales con hasta 50% de bipartición; GC (n=4) grupo de control, sin bipartición. Todos ellos fueron sometidos a la medición de la temperatura rectal, termografía corporal y escrotal. Los exámenes ultrasonográficos Doppler se realizaron en el plexo pampiniforme. Adicionalmente los animales fueron sometidos a la punción biopsia aspirativa testicular (PBA) y 2 eyaculaciones de cada reproductor fueron cosechados para evaluación espermática. No se observaron diferencias para los resultados de la PBA, de la termografía escrotal y del espermograma comparando los dos grupos. En la evaluación hemodinámica sólo el IR (0,53a +- 0,11 y 0,46b +- 0,06, p = 0,0108) fue superior para los animales bipartidos. Se concluye que los caprinos que tieren hasta 50% de bipartición escrotal tienen calidad espermática similares a animales no bipartidos, no tienen menor temperatura testicular y mayor número de células germinativas primordiales como se suponía en la hipótesis inicial de la investigación.(AU)

Assuntos

RESUMO

A espécie caprina tornou-se um excelente modelo animal objetivando a produção de proteínas recombinantes no leite. A obtenção de machos fundadores transgênicos é importante, pois os mesmos permitem aumentar o número de crias transgênicas pelo simples uso da inseminação artificial. Este trabalho teve por objetivo, comparar parâmetros seminais de machos caprinos transgênicos (TG) e não transgênicos (NTG) para o Fator Estimulante de Colônia de Granulócitos humano (hG-CSF). Para tanto, semanalmente,foram colhidas amostras de sêmen de todos os animais através de vagina artificial. Foram avaliados os seguintes parâmetros: volume, motilidade massal, concentração espermática, motilidade individual progressiva (MIP), vigor e patologias espermáticas. Foram observadas diferenças estatísticas somente para volume e MIP, os quais tiveram valores superiores (p0,05). Em conclusão, a presença do transgene não afetou a qualidade do sêmen favorecendo e contribuindo para a formação de um rebanho transgênico.

Goats became an excellent animal model aiming the production of recombinant proteins in the milk. Transgenic founder males are important to increase the number of transgenic kids by artificial insemination. This work aimed to compare seminal parameters of transgenic (TG) and non-transgenic goats (NTG) for the human Granulocyte Colony Stimulating Factor (hG-CSF). Thus, weekly, semen samples were collected from all transgenic males by artificial vagina and evaluated: volume, mass motility, sperm concentration, individual progressive motility (IPM), vigor and sperm pathologies. Statistical differences were observed only for volume and IPM, which had higher values (p0.05). In conclusion, the presence of the transgene did not affect the semen quality and these animals can be used to obtain kids and enable to create of a transgenic herd.

Assuntos

RESUMO

The objectives of this twelve-week feeding trial were to determine the effects of dietary Saccharomyces cerevisiae and/or zinc oxide on epididymal sperm characteristics, testicular morphometric traits, and growth parameters of bucks. 16 (New Zealand White) bucks 16-wk-old, weighing 2.8kg were randomly allotted to one of 4 treatment groups. Each treatment was replicated four times, with 1 buck per replicate, in a completely randomized design (CRD). Each treatment group was randomly assigned to one of the four commercial experimental diets designated thus: TA = control diet with no additives, TB = 0.12g/kg Saccharomyces cerevisiae, TC = 150mg/kg zinc oxide and TD = 0.12g/kg Saccharomyces cerevisiae +150 mg/kg zinc oxide. Although treatment had no effect (p>0.05) on final body weight, average daily weight gain and feed conversion ratio, epididymal sperm characteristics and testicular morphometric traits differed significantly (p 0.05). Bucks on TB(Saccharomyces cerevisiae-based diet) had improved (p 0.05) sperm concentration, motility and live sperm, tubule diameter, epididymal volume, volume fraction of duct, and total duct volume, but decreased testicular volume. Bucks fed TA (control diet) had improved volume fraction of tubule but recorded the highest incidence of head and tail sperm abnormality. Though, TC(zinc oxide-based diet) enhanced (p 0.05) seminal vesicle volume, sperm pH was better among bucks fed TD (Saccharomyces cerevisiae + zinc oxide-based diet). It can be concluded that dietary inclusion of Saccharomyces cerevisiae at 0.12g/kg of feed improved epididymal sperm characteristics and testicular morphometric traits of rabbit bucks.(AU)

Assuntos

RESUMO

A espécie caprina tornou-se um excelente modelo animal objetivando a produção de proteínas recombinantes no leite. A obtenção de machos fundadores transgênicos é importante, pois os mesmos permitem aumentar o número de crias transgênicas pelo simples uso da inseminação artificial. Este trabalho teve por objetivo, comparar parâmetros seminais de machos caprinos transgênicos (TG) e não transgênicos (NTG) para o Fator Estimulante de Colônia de Granulócitos humano (hG-CSF). Para tanto, semanalmente,foram colhidas amostras de sêmen de todos os animais através de vagina artificial. Foram avaliados os seguintes parâmetros: volume, motilidade massal, concentração espermática, motilidade individual progressiva (MIP), vigor e patologias espermáticas. Foram observadas diferenças estatísticas somente para volume e MIP, os quais tiveram valores superiores (p<0,05) nos animais TG. Observou-se também, em ambos os grupos, um pequeno percentual de patologias espermáticas (<2%), sem diferença estatística (p>0,05). Em conclusão, a presença do transgene não afetou a qualidade do sêmen favorecendo e contribuindo para a formação de um rebanho transgênico.(AU)

Goats became an excellent animal model aiming the production of recombinant proteins in the milk. Transgenic founder males are important to increase the number of transgenic kids by artificial insemination. This work aimed to compare seminal parameters of transgenic (TG) and non-transgenic goats (NTG) for the human Granulocyte Colony Stimulating Factor (hG-CSF). Thus, weekly, semen samples were collected from all transgenic males by artificial vagina and evaluated: volume, mass motility, sperm concentration, individual progressive motility (IPM), vigor and sperm pathologies. Statistical differences were observed only for volume and IPM, which had higher values (p<0.05) in TG animals. There were also a small percentage of sperm pathologies (<2%) in both groups, with no statistical difference (p>0.05). In conclusion, the presence of the transgene did not affect the semen quality and these animals can be used to obtain kids and enable to create of a transgenic herd.(AU)

Assuntos

RESUMO

The objectives of this twelve-week feeding trial were to determine the effects of dietary Saccharomyces cerevisiae and/or zinc oxide on epididymal sperm characteristics, testicular morphometric traits, and growth parameters of bucks. 16 (New Zealand White) bucks 16-wk-old, weighing 2.8kg were randomly allotted to one of 4 treatment groups. Each treatment was replicated four times, with 1 buck per replicate, in a completely randomized design (CRD). Each treatment group was randomly assigned to one of the four commercial experimental diets designated thus: TA = control diet with no additives, TB = 0.12g/kg Saccharomyces cerevisiae, TC = 150mg/kg zinc oxide and TD = 0.12g/kg Saccharomyces cerevisiae +150 mg/kg zinc oxide. Although treatment had no effect (p>0.05) on final body weight, average daily weight gain and feed conversion ratio, epididymal sperm characteristics and testicular morphometric traits differed significantly (p 0.05). Bucks on TB(Saccharomyces cerevisiae-based diet) had improved (p 0.05) sperm concentration, motility and live sperm, tubule diameter, epididymal volume, volume fraction of duct, and total duct volume, but decreased testicular volume. Bucks fed TA (control diet) had improved volume fraction of tubule but recorded the highest incidence of head and tail sperm abnormality. Though, TC(zinc oxide-based diet) enhanced (p 0.05) seminal vesicle volume, sperm pH was better among bucks fed TD (Saccharomyces cerevisiae + zinc oxide-based diet). It can be concluded that dietary inclusion of Saccharomyces cerevisiae at 0.12g/kg of feed improved epididymal sperm characteristics and testicular morphometric traits of rabbit bucks.

Assuntos

RESUMO

The aim of this work was to evaluate the in vitro effect of adding Trolox in freezing extender for goat semen. Ejaculates from five bucks were evaluated, and when approved, the samples were pooled, diluted according to experimental groups [Trolox 0 (control), 30, 60 and 120 nmol ml(-1) ] and frozen in an automated system. Thawed samples (37 °C/30 s) were evaluated for plasma membrane (PMi) and acrosome integrity (Aci), mitochondrial membrane potential (MMP) and sperm kinematics by CASA system. Spermatozoa ultrastructure was evaluated in fresh and post-thawed semen. No significant difference (P > 0.05) was observed among control and Trolox groups in the analyses of PMi, Aci, MMP and CASA in goat spermatozoa after thawing. Samples of 60 and 120 nmol ml(-1) Trolox groups had a higher percentage of cells that had intact plasma membranes in spermatozoa head than in the other groups, although they did not differ (P > 0.05) before being frozen. A higher percentage (P < 0.05) of spermatozoa with intact mitochondria was observed in fresh semen, control and Trolox 60 nmol ml(-1) groups than in the other groups. Addition of Trolox to skim milk extender at 60 nmol ml(-1) ultrastructurally preserves the plasma membrane and mitochondrial sheath integrity in goat spermatozoa after cryopreservation.

Assuntos