RESUMO

PURPOSE: The aim of this study was to assess the ability of ultrasound biomicroscopy (UBM) to diagnose the initial stages of nonalcoholic fatty liver disease (NAFLD) in a rat model. METHODS: Eighteen male Wistar rats were allocated to control or experimental groups. A high-fat diet (HFD) with 20% fructose and 2% cholesterol, resembling a common Western diet, was fed to animals in the experimental groups for up to 16 weeks; those in the control group received a regular diet. A 21 MHz UBM system was used to acquire B-mode images at specific times: baseline (T0), 10 weeks (T10), and 16 weeks (T16). The sonographic hepatorenal index (SHRI), based on the average ultrasound image gray-level intensities from the liver parenchyma and right renal cortex, was determined at T0, T10, and T16. The liver specimen histology was classified using the modified Nonalcoholic Steatohepatitis Clinical Research Network NAFLD activity scoring system. RESULTS: The livers in the animals in the experimental groups progressed from sinusoidal congestion and moderate macro- and micro-vesicular steatosis to moderate steatosis and frequent hepatocyte ballooning. The SHRI obtained in the experimental group animals at T10 and T16 was significantly different from the SHRI of pooled control group. No significant difference existed between the SHRI in animals receiving HFD between T10 and T16. CONCLUSION: SHRI measurement using UBM may be a promising noninvasive tool to characterize early-stage NAFLD in rat models.

RESUMO

Objetivos: Evaluar la relación entre el colesterol dietario, consumo de huevo y el perfíl lipídico en adultos aparentemente sanos, según grupo etario. Diseño: Estudio descriptivo, de asociación cruzada. Institución: Centro de Investigación de Bioquímica y Nutrición, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú. Participantes: Adultos aparentemente sanos. Intervenciones: En 50 adultos aparentemente sanos se determinó el consumo de colesterol dietario, huevo y perfil lipídico. Resultados: Trece participantes consumían menos de 3 huevos/semana, 19 de 3 a 4 huevos/semana y 18 más de 4 huevos/semana. En relación al consumo de colesterol dietario, el grupo de 20 a 29 años presentó un promedio de ingesta (358,9±152,5 mg/dL) significativamente superior (p<0,05) al grupo de 30 a 39 años (223 ± 82,8 mg/dL). El colesterol (col) total de los tres grupos según consumo de huevo fue 179,8 ± 52, 168,8 ± 44 y 164,3 ± 62 mg/dL, respectivamente; para el HDL-col se obtuvo 38,9 ± 15, 28,0 ± 15 y 35,2 ± 14 mg/dL, respectivamente; para el LDL-col, 118,1±41, 112,5 ± 39 y 100,4 ± 47,9 mg/dL, respectivamente; para los triglicéridos, 199,2 ± 169, 152,8 ± 108 y 124,2 ± 92 mg/dL, respectivamente. Conclusiones: El consumo de colesterol dietario y huevo en la muestra estudiada no tuvo relación con el perfil lipídico.

Objectives: To determine the relationship between dietary cholesterol, egg consumption and lipid profile in apparently healthy adults according to age group. Design: Descriptive cross-association study. Setting: Biochemistry and Nutrition Research Center, Faculty of Medicine, Universidad Nacional Mayor de San Marcos. Participants: Apparently healthy adults. Interventions: In 50 apparently healthy adults dietary cholesterol consumption, egg and lipid profile were determined. Results: Thirteen participants consumed less than 3 eggs / week, 19 consumed from 3-4 eggs / week and 18 consumed more than 4 eggs / week. The 20-29 year-old group had an average intake of dietary cholesterol (358,9 ± 152,5 mg / dL) significantly higher (p <0,05) than the group 30-39 year-old (223 ± 82, 8 mg / dL). Total cholesterol (chol) of the three groups according to egg consumption was respectively 179,8 ± 52, 168,8 ± 44, and 164,3 ± 62 mg / dL; for HDL-chol 38,9 ± 15 , 28,0 ± 15, and 35,2 ± 14 mg / dL; for LDL-chol 118,1 ± 41, 112,5 ± 39, and 100,4 ± 47,9 mg / dL; for triglycerides 199, 2 ± 169, 152,8 ± 108, 124,2 ± 92 mg / dL. Conclusions: The consumption of dietary cholesterol and eggs in the sample was not related to the lipid profile.