RESUMO
The objective of this study was to evaluate the antioxidant activity, determine and quantify the phenolic compounds and other compounds, and evaluate the cellular cytotoxicity of mycelium extracts of two new Basidiomycete mushrooms strains isolated in Brazil and identified as Lepista sordida GMA-05 and Trametes hirsuta GMA-01. Higher amounts of proteins, free amino acids, total and reducing carbohydrates, and phenolic compounds as chlorogenic, ferulic, caffeic, and gallic acids were found in extracts of T. hirsuta and L. sordida. Protocatechuic acid was found only in aqueous extracts of L. sordida. The TLC of the extracts showed the predominance of glucose and smaller amounts of xylose. It was observed through UPLC-MS higher amounts of phenolic compounds. The aqueous extract from T. hirsuta had the most noteworthy results in the antioxidant assays, especially the ABTS test. The cytotoxic activity was evaluated using two different cell lineages and showed higher toxicity for L. sordida in macrophages J774-A1. However, in Vero cells, it was 12.6-fold less toxic when compared to T. hirsuta. Thus, both mushrooms show potential as functional foods or additives, presenting phenolic content, antioxidant activity, and low cytotoxic activity in the tested cells.
Assuntos
Agaricales , Trametes , Animais , Antioxidantes/análise , Antioxidantes/farmacologia , Brasil , Chlorocebus aethiops , Cromatografia Líquida , Micélio/química , Extratos Vegetais/química , Polyporaceae , Espectrometria de Massas em Tandem , Trametes/química , Células VeroRESUMO
Some ultrastructural and cytochemical aspects of Lepista sordida are described. Basidiospores with verruculose and irregular ornamentations were observed through scanning electron microscopy. Confocal microscopy indicated that most of them exhibited a single large lipid body, no acidic vesicles and nucleus localized in the cell periphery.
Este trabalho descreve aspectos ultraestruturais e citoquímicos de Lepista sordida. Basidiósporos recobertos com ornamentações verruculosas e irregulares foram observados através de microscopia eletrônica de varredura. Através de microscopia confocal verificou-se que a maioria apresentava um grande corpo lipídico, ausência de vesículas ácidas e núcleo localizado na periferia.
Assuntos
Agaricales/isolamento & purificação , Basidiomycota/isolamento & purificação , Esporos Fúngicos/isolamento & purificação , Técnicas In Vitro , Métodos , Microscopia Confocal , Microscopia Eletrônica de VarreduraRESUMO
Some ultrastructural and cytochemical aspects of Lepista sordida are described. Basidiospores with verruculose and irregular ornamentations were observed through scanning electron microscopy. Confocal microscopy indicated that most of them exhibited a single large lipid body, no acidic vesicles and nucleus localized in the cell periphery.
RESUMO
Some ultrastructural and cytochemical aspects of Lepista sordida are described. Basidiospores with verruculose and irregular ornamentations were observed through scanning electron microscopy. Confocal microscopy indicated that most of them exhibited a single large lipid body, no acidic vesicles and nucleus localized in the cell periphery.
Este trabalho descreve aspectos ultraestruturais e citoquímicos de Lepista sordida. Basidiósporos recobertos com ornamentações verruculosas e irregulares foram observados através de microscopia eletrônica de varredura. Através de microscopia confocal verificou-se que a maioria apresentava um grande corpo lipídico, ausência de vesículas ácidas e núcleo localizado na periferia.
RESUMO
A Lepista sordida laccase has been characterized. Laccase and manganese peroxidase were detected in liquid medium with ammonium phosphate, yeast extract and ammonium molybdidate as nitrogen sources after 3 days of cultivation. Laccase optimal temperature and pH were 45ºC and 3.5, respectively.
Uma lacase de Lepista sordida foi caracterizada. O fungo produziu lacase e manganês peroxidase em meio líquido com fosfato de amônio, extrato de levedura e molibdato de amônio como fontes de nitrogênio 3 dias após a inoculação. Temperatura e pH ótimos para lacase foram 45ºC e 3,5, respectivamente.