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The purpose of this study was to evaluate the influence of the association of Opuntia stricta (OS) or Nopalea cochenillifera (NC) with Moringa hay (MY) or Leucaena hay (LY) on nutrient intake, ingestive behavior, serum biochemistry, and milk production and composition of dairy goat. Eight multiparous Saanen goats were used, with an average of 68 ± 8 days in milk and 48.5 ± 6 kg of initial weight, fed with the following treatment diets: OS + MY; OS + LY; NC + MY, and NC + LY. The treatments were distributed in a double Latin square, in a 2 × 2 factorial scheme (two varieties of cactus and two of hay). Goats fed with NC had higher (P < 0.05) intakes of dry matter, crude protein, neutral detergent fiber (NDF), and non-fibrous carbohydrates. There was no influence (P > 0.05) on milk production, but goats fed with OS produced milk with higher (P < 0.05) fat content and presented higher (P < 0.05) feed efficiency. There was no effect (P > 0.05) of the hay type on the nutrient intake and milk production and composition of goats, but the animals fed with MY had higher (P < 0.05) serum cholesterol levels and greater efficiency of NDF rumination. Goats fed with OS showed greater feeding and rumination efficiency. Our results indicated that both cactus species could be successfully included in the diet of low yielding dairy goats fed 18.4% concentrate.

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Cactus pear cladodes, clones 'Miúda' (MIU) and 'Orelha de Elefante Mexicana' (OEM) were harvested at 6 am and 8 pm during the rainy-dry, dry and rainy seasons to evaluate the effect of type of clone and harvest seasons on the physicochemical and technological properties of mucilage as well as the optical, physicochemical, mechanical, thermal and microstructural characteristics of the films obtained. The mucilage of the OEM clone presented a higher content of phenolic compounds, compared to the Nopalea genus, regardless of the season and time of harvest. Furthermore, the dry period resulted in higher carbohydrate levels, regardless of the harvest time. The biopolymeric films produced from the OEM clone harvested in the rainy season and rainy-dry transition showed darker color, better mechanical properties, water barrier, compact microstructure and thermal stability when compared to the MIU clone. Furthermore, harvesting at 6 am provided improvements in the mechanical conditions, permeability and thermal stability of the films of both types of clones studied. These results showed strong environmental modulation, naturally incorporating important macromolecules such as carbohydrates and phenolic compounds, used in the industry in the production of nutraceutical foods, into the mucilage. Furthermore, harvesting cladodes at 6 am in the rainy and transitional (rainy-dry) periods provided better quality biopolymeric films and/or coatings.

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Our hypothesis is that the total replacement of ground corn grain (GCG) by cactus pear (CP) in association with 70% of leucaena hay (LH) increases dry matter (DM) intake and digestibility and nitrogen metabolism and maintains the feeding behavior of cattle. The objective was to evaluate the effects of inclusion of these feeds on the nutritional parameters and nitrogen metabolism of cattle. Eight steers, average age of 12 months, weight of 267.5 ± 15 kg, were distributed into two simultaneous 4 × 4 Latin squares according to a 2 × 2 factorial scheme, in which the first factor evaluated was different proportions of inclusion of LH (50 or 70% DM basis), and the second factor was the total replacement of GCG with CP. There was lower DM intake in animals fed with 70% LH. There was an interaction between the factors for DM digestibility, in which the animals fed CP did not differ regardless of the inclusion of LH, but for animals without CP, the greater inclusion of LH decreases DM digestibility. The inclusion of CP decreased free water intake. There was an interaction for total water intake (WI), in which the animals fed without CP presented similarity for this, while in the inclusion of CP, the animals fed 70% of LH presented higher WI. Also, lower values of chewing were observed for animals fed with CP. The replacement of GCG with CP improves the efficiency of nitrogen utilization in the rumen. The inclusion of 70% LH depresses intake.

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The production of mixed silages can be an essential strategy for storing and processing feed for ruminants in arid and semi-arid regions. This study aimed to evaluate the effect of inclusion levels of forage cactus in sorghum silage on chemical-bromatological composition, losses, in vitro digestibility, and fermentative profile. Sorghum silages were produced by adding 0, 10, 20, 30, and 40% forage cactus based on natural matter. Experimental silos were filled with the mixtures and hermetically sealed. The silos were opened after 34 days of fermentation, and the samples were analyzed for composition, digestibility, fermentative profile, and losses in silage. The inclusion of forage cactus presented a negative quadratic influence (P < 0.05) on the contents of dry matter (DM), ether extract, neutral detergent fiber, acid detergent fiber, and cellulose of sorghum silage. The DM concentration decreased from 35.83 to 25.43% for the control treatment (0%) to the 20% treatment, followed by stabilization at values close to 26 ± 1% in subsequent levels. Digestibility in vitro and the total digestible nutrients of the silages increased linearly (P < 0.05) with the inclusion of forage cactus. However, there was a linear increase (P < 0.05) of pH, ammonia nitrogen, acetic acid, and butyric acid with the inclusion of forage cactus, reaching values indicative of the limitation of the fermentation process from the level of 20% inclusion. Including forage cactus in sorghum silage did not affect (P > 0.05) losses by gases, effluents, and DM recovery from silage. Adding up to 10% of forage cactus can benefit chemical-bromatological characteristics, digestibility, and fermentation of sorghum silage.(AU)

A produção de silagens mistas pode ser importante estratégia para armazenamento e beneficiamento dos alimentos para ruminantes em regiões áridas e semiáridas. Objetivou-se avaliar efeito de níveis de inclusão de palma forrageira na ensilagem do sorgo sobre composição químico-bromatológica, perdas, digestibilidade in vitro e perfil fermentativo. Foram produzidas silagens de sorgo com adição de 0, 10, 20, 30 e 40% de palma forrageira com base na matéria natural. Utilizou-se silos experimentais preenchidos com as misturas e fechados hermeticamente. Após 34 dias de fermentação os silos foram abertos e as amostras analisada quanto a composição, digestibilidade, perfil fermentativo e perdas na ensilagem. A inclusão de palma forrageira influenciou de forma quadrática negativa (P < 0,05) os teores de matéria seca (MS), extrato etéreo, fibra em detergente neutro, fibra em detergente ácido e celulose da silagem de sorgo. The DM concentration decreased from 35.83 to 25.43% for the control treatment (0%) to the 20% treatment, followed by stabilization at values close to 26 ± 1% in subsequent levels. A digestibilidade in vitro e os nutrientes digestíveis totais das silagens aumentaram linearmente (P < 0,05) com a inclusão de palma forrageira. Contudo, houve incremento linear (P < 0,05) do pH, nitrogênio amoniacal, ácido acético e ácido butírico com a inclusão de palma forrageira, atingido valores indicativos de limitação do processo fermentativo a partir do nível de 20% de inclusão. A inclusão de palma forrageira na ensilagem do sorgo não influenciou (P > 0,05) as perdas por gases, efluentes, e recuperação de MS da silagem. A adição até 10% de palma forrageira pode ser recomendada para beneficiar características químico-bromatológica, digestibilidade e fermentação da silagem de sorgo.(AU)

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This study aimed to evaluate the effects of four varieties of cactus pear resistant to carmine cochineal as exclusive roughage for lambs on the biochemical, histopathological, and histomorphometric parameters of kidneys. Twenty-four castrated male crossbred lambs at eight months of age and an initial body weight of 21.0 ± 0.5 kg were distributed in a completely randomized design, with four treatments and six repetitions. The experimental treatments consisted of four diets containing Miúda cactus pear, IPA-Sertânia cactus pear, IPA-F21 cactus pear, or Orelha de Elefante Mexicana (OEM) cactus pear as the only roughage. Blood samples were collected every two weeks (14 d, 28 d, 42 d, and 56 d) to quantify serum urea and creatinine levels. After 72 days of the introduction of the tested diets, the animals were slaughtered and fragments of the kidneys were collected for histological analysis. The serum urea level was higher in animals fed a diet based on the Miúda variety (49.38 mg dL-1), and the serum creatinine levels were lower in the last two collections (P = 0.009). The most frequent histopathological findings in the kidneys were calcification, congestion, glomerular atrophy, presence of luminal cellular debris, and nephrosis, regardless of the cactus pear variety. The Miúda cactus pear and OEM cactus pear varieties caused more severe damage to the nephron components, while the varieties IPA F-21 and IPA-Sertânia caused less significant injuries. The use of IPA-Sertânia and IPA F-21 cactus pear varieties is suggested in lamb's diets, due to the lower impact on the renal parenchyma. However, there was no expressive impairment of renal function, and there was no difference between the cactus pear varieties tested in this study on the weight gain of the animals, and they can all be used to feed feedlot sheep.

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Leaves of Croton argyrophyllus contain essential oil with promising active components for the development of drugs and botanical insecticides. In this study, we evaluated the enzymatic pretreatment process to increase the extraction of essential oil from fresh and dried leaves of C. argyrophyllus. Pretreatment was carried out using a crude multienzymatic extract obtained via solid-state fermentation of forage palm by Aspergillus niger, and the extraction was performed by hydrodistillation. A Doehlert matrix was used to optimize the enzymatic pretreatment variables temperature and enzymatic extract. The effect of pretreatment time was also investigated. At optimum experimental conditions, 41.34°C, 140 min, and 130.73 mL of enzyme in 369.27 mL of water, the essential oil yield from fresh leaves subjected to enzymatic pretreatment increased by 9.35% and that from dry leaves by 6.77%. Based on chromatographic analysis (GC-MS), no compound was degraded in the extraction process. Micromorphological analysis confirmed the rupture of the glandular trichomes, favoring essential oil release. Therefore, enzymatic pretreatment associated with hydrodistillation increased the essential oil yield and is a promising application to obtain essential oil for therapeutic purposes without altering its composition.

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The objective was to evaluate the effect of different levels of potassium fertilization on the initial growth of forage cactus (Nopalea cochenillifera Salm Dyck) clones. The experiment lasted six months, arranged in a randomized block design, in a 5 x 2 factorial scheme, with five levels of potassium fertilization (0, 250, 500, 750, and 1,000 kg of K2O ha-1) and two forage cactus clones (Little Sweet and Giant Sweet), with four replicates. One cladode was planted per pot (experimental unit) with a capacity of 7 kg of soil (24 x 17 x 23 cm). During the experimental period, the plants' structural characteristics were evaluated, such as plant height and width, length, width, thickness, and perimeter of the cladode, as well as accounting for the number of total cladodes, and in order of appearance. At the end of the experiment, biomass was evaluated to determine the fresh matter (FM) and dry matter (DM) yield of the aerial part and the root system. After determining the DM, the potassium content in the plant tissues was determined. The data were submitted to ANOVA and, if necessary, to the Tukey test at a 5% significance level. The potassium fertilization and the forage palm clones did not exert influences based on the variables evaluated during the experimental conduction. Potassium fertilization does not influence the structural and productive characteristics and the potassium content in the N. cochenillifera clones' initial growth.(AU)

Objetivou-se avaliar o efeito de diferentes níveis de adubação potássica no crescimento inicial de clones de palma forrageira (Nopalea cochenillifera Salm Dyck). O experimento foi conduzido durante seis meses, disposto em delineamento em blocos ao acaso, em esquema fatorial 5x2, sendo cinco níveis de adubação potássica (0, 250, 500, 750 e 1000 Kg de K2O ha-1) e dois clones de palma forrageira (Doce Gigante e Doce Miúda), com quatro repetições. Foi plantado um cladódio por vaso (unidade experimental) com capacidade para 7 kg de solo (24 x 17 x 23 cm). Ao longo do período experimental, foram avaliadas as características estruturais das plantas, tais como altura e largura de planta, comprimento, largura, espessura e perímetro de cladódio, bem como contabilização do número de cladódios total e por ordem de surgimento. Ao final do experimento avaliou-se a biomassa, para determinação do rendimento de massa fresca (MV) e seca (MS) da parte aérea e do sistema radicular. Após a determinação de MS, determinou-se o teor potássico nos tecidos vegetais. Os dados foram submetidos a ANOVA e, caso necessário, ao teste de Tukey a 5% de significância. A adubação potássica e os clones de palma forrageira não exerceram influências significativas nas variáveis avaliadas durante a condução experimental. A adubação potássica não influencia as características estruturais, produtivas e teor potássico no crescimento inicial de clones de Nopalea cochenillifera.(AU)

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This study aimed to investigate the effects on nutrient intake and digestibility, milk yield (MY) and composition, milk fatty acids profile, and microbial protein synthesis caused by feeding lactating dairy cows four different forage cactus genotypes. Eight Girolando cows (5/8 Holstein × 3/8 Gyr), weighing 490 ± 69.0 kg (means ± standard deviation), and producing 15.5 ± 1.0 kg/d of milk during pretrial were distributed to two contemporaneous 4 × 4 Latin squares. The cows were fed a total mixed ration composed of sorghum silage (385 g/kg of dry matter (DM)), concentrated mix (175 g/kg DM), and forage cactus (440 g/kg DM). The experimental treatments consisted of different cactus genotypes, such as Gigante cactus (GC), Miúda cactus (MC), IPA Sertânia cactus (SC), and Orelha de Elefante Mexicana cactus (OEMC). The feeding of MC provided a higher intake of DM, organic matter (OM), and total digestible nutrients, as well as higher MY, energy-corrected milk, and microbial protein synthesis in comparison with those resulting from the other genotypes tested. The GC promoted lower DM and OM, and the apparent digestibility of neutral detergent fiber. The cows fed with OEMC showed lower MY and milk protein yield and content, and higher unsaturated over saturated fatty acid ratio in milk. Miúda forage cactus increased nutrient intake, digestibility of DM and OM, and microbial synthesis without impairing the milk fatty acid profile.

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The objective of this study was to evaluate the influence of the total or partial replacement of Tifton 85 hay (TH) by cassava foliage hay (CH) and/or spineless cactus (SC) on the carcass characteristics and meat quality of confined lambs. Thirty-five Dorper x Santa Inês crossbred lambs were used, with an initial average weight of 16.0 ± 1.9 kg, fed with five treatment diets: 70% TH, 70% CH, 35% TH + 35% CH, 35% TH + 35% SC, and 35% CH + 35% SC. After 90 days of confinement, the animals were slaughtered and the carcass and meat data were compared using the Tukey test at 5%. The partial replacement of TH by SC increased the cold carcass weight by 3.9 kg (p < .05). The proportion of fat (%) increased (p < .05) when TH was replaced by CH + SC. The intensity of red (a*) was lower (p < .05) in the meat of animals fed with CH or CH + SC. It is recommended to partially replace TH with SC, or to replace totally with CH in association with SC.

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The present study was conducted to evaluate the intake, digestibility and nitrogen balance of diets for finishing sheep, containing leucaena hay as a source of effective fibre and forage palm. Twenty-one male sheep were used in a randomized block design with three treatments, represented by total diets, where the forage was leucaena hay and forage palm in natura in proportions 350:650, 450:550 and 550:450 g/kg respectively. For the pH and NH3 -N in the ruminal fluid, and urea in the blood serum, plots split in time were adopted. Dry matter and nutrient digestibility, and the nitrogen balance were not influenced (p > .05) by the diets. The neutral detergent fibre digestibility decreased (p < .05) as the proportions of leucaena hay in the diets increased. The pH and NH3 -N were suitable for ruminal fermentation, and blood urea was maintained under normal physiological conditions for sheep. Combining 350 to 550 g/kg of leucaena hay with 450 to 650 g/kg of forage palm in total diets with a forage:concentrate ratio of 60:40 in late-maturing sheep meets the nutritional requirements for daily gain 200 g, characterizing leucaena hay as important source of effective fibre and nutrients in diets.

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