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Abstract Objective To investigate the relationship between lactate acid level and hospitalization mortality in neonatal necrotizing enterocolitis (NEC). Method Paediatric-specific critical care database collected clinical data from the intensive care unit of Children's Hospital Affiliated to Zhejiang University Medical College from 2010 to 2018. Clinical and laboratory examination information of NEC patients was collected and divided into the death group and discharge group to find out the risk factors affecting the prognosis through univariate and multivariate analysis. Results Among 104 NEC neonates, the admission age was 7.5 days and the weight was 2.03 kg. Comparing the death group with the discharge group, there were significant differences in therapeutic regimen, pH, serum albumin, total protein, creatinine and lactate acid. Multivariate and threshold effect analysis showed that lactate acid had a linear correlation with hospital mortality, and newborns who died in the hospital had much higher lactate levels than those who were discharged. The mortality of NEC newborns increased by 40-45% for every 1 mmol/L increase in lactate acid level. Conclusions There was a correlation between lactate acid level and hospital mortality in newborns with NEC, and lactate acid level was an important index to evaluate the prognosis of NEC.
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OBJECTIVE: To investigate the relationship between lactate acid level and hospitalization mortality in neonatal necrotizing enterocolitis (NEC). METHOD: Paediatric-specific critical care database collected clinical data from the intensive care unit of Children's Hospital Affiliated to Zhejiang University Medical College from 2010 to 2018. Clinical and laboratory examination information of NEC patients was collected and divided into the death group and discharge group to find out the risk factors affecting the prognosis through univariate and multivariate analysis. RESULTS: Among 104 NEC neonates, the admission age was 7.5 days and the weight was 2.03 kg. Comparing the death group with the discharge group, there were significant differences in therapeutic regimen, pH, serum albumin, total protein, creatinine and lactate acid. Multivariate and threshold effect analysis showed that lactate acid had a linear correlation with hospital mortality, and newborns who died in the hospital had much higher lactate levels than those who were discharged. The mortality of NEC newborns increased by 40-45% for every 1 mmol/L increase in lactate acid level. CONCLUSIONS: There was a correlation between lactate acid level and hospital mortality in newborns with NEC, and lactate acid level was an important index to evaluate the prognosis of NEC.
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Enterocolite Necrosante , Doenças do Recém-Nascido , Recém-Nascido , Humanos , Criança , Peso ao Nascer , Estudos Retrospectivos , Enterocolite Necrosante/diagnóstico , Idade Gestacional , Fatores de Risco , Prognóstico , Cuidados CríticosRESUMO
A implementação das PICs no Brasil é uma realidade, dessa forma, compreende-se que o enfermeiro é o profissional que em tese deve possuir habilidades para aplicar as técnicas em pacientes. Com base nisso, o objetivo do trabalho foi descrever a atuação da enfermagem através de Planos de ações por intermédio das PICs em individuos com diminuição da qualidade de vida em razão do desenvolvimento de doenças crônicas degenerativas. Dessa forma, o presente artigo trata-se de uma revisão integrativa de literatura. Os resultados obtidos mostram que a aplicabilidade das PICs, está consolidada, sendo uma prática bastante utilizada na intervenção terapêutica de indivíduos portadores de doenças crônicas degenerativas, na qual as mais utilizadas são: plantas medicinais, reiki, homeopatia, acupuntura e auricuoterapia, entretanto, em relação a assistência de enfermagem, foi observado impasses em relação a capacitação profissional. Logo, concluímos que com base nas produções cientificas existentes a respeito das PICs, a prática infere em diversos benefícios ao indivíduo, estas que se convergem a promoção de maior qualidade de vida ao paciente com doenças crônicas, porém, a falta de capacitação profissional revela um impasse ainda persistente.
The implementation of PICs in Brazil is a reality, therefore, it is understood that the nurse is the professional who, in theory, must have the skills to apply the techniques to patients. Based on this, the objective of the study was to describe the role of nursing through Action Plans through PICs in individuals with reduced quality of life due to the development of chronic degenerative diseases. Thus, this article is an integrative literature review. The results obtained show that the applicability of PICs is consolidated, being a practice widely used in the therapeutic intervention of individuals with chronic degenerative diseases, in which the most used are: medicinal plants, reiki, homeopathy, acupuncture and auricutherapy, however, in regarding nursing care, impasses regarding professional training were observed. Therefore, we conclude that based on the existing scientific productions regarding PICs, the practice infers in several benefits to the individual, these that converge to the promotion of a better quality of life for the patient with chronic diseases, however, the lack of professional training reveals a stalemate still persistent.
La implementación de los PICs en Brasil es una realidad, por lo tanto, se entiende que la enfermera es el profesional que, en teoría, debe tener las habilidades para aplicar las técnicas a los pacientes. Con base en esto, el objetivo del estudio fue describir el papel de la enfermería a través de Planes de Acción por medio de PICs en individuos con calidad de vida reducida debido al desarrollo de enfermedades crónico degenerativas. Así, este artículo es una revisión bibliográfica integradora. Los resultados obtenidos muestran que la aplicabilidad de los PICs está consolidada, siendo una práctica ampliamente utilizada en la intervención terapéutica de individuos con enfermedades crónicas degenerativas, en la que las más utilizadas son: plantas medicinales, reiki, homeopatía, acupuntura y auricuterapia, sin embargo, en lo que respecta a los cuidados de enfermería, se observaron impasses en cuanto a la formación profesional. Por lo tanto, concluimos que con base en las producciones científicas existentes en relación a las PICs, la práctica infiere en varios beneficios al individuo, estos que convergen a la promoción de una mejor calidad de vida para el paciente con enfermedades crónicas, sin embargo, la falta de formación profesional revela un impasse aún persistente.
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Terapias Complementares/enfermagem , Doença Crônica/enfermagem , Doença Crônica/tratamento farmacológico , Qualidade de Vida , Terapias Complementares/instrumentação , Terapias Complementares/métodos , Revisão , Capacitação Profissional , Enfermeiras e Enfermeiros , Cuidados de EnfermagemRESUMO
A therapeutic strategy for prostate cancer (PCa) involves the use of 9-cis-retinoic acid (9cRA) to induce cancer stem cells (CSCs) differentiation and apoptosis. Polyinosinic:polycytidylic acid (PIC) is a Toll-like receptor 3 (TLR3) agonist that induces tumor cells apoptosis after activation. PIC+9cRA combination activates retinoic acid receptor ß (RARß) re-expression, leading to CSC differentiation and growth arrest. Since inorganic arsenic (iAs) targets prostatic stem cells (SCs), we hypothesized that arsenic-transformed SCs (As-CSCs) show an impaired TLR3-associated anti-tumor pathway and, therefore, are unresponsive to PIC activation. We evaluated TLR3-mediated activation of anti-tumor pathway based in RARß expression, on As-CSC and iAs-transformed epithelial cells (CAsE-PE). As-CSCs and CAsE-PE showed lower TLR3 and RARß basal expression compared to their respective isogenic controls WPE-Stem and RWPE-1. Also, iAs transformants showed reduced expression of mediators in TLR3 pathway. Importantly, As-CSCs were irresponsive to PIC+9cRA in terms of increased RARß and decreased SC-markers expression, while CAsE-PE, a heterogeneous cell line having a small SC population, were partially responsive. These observations indicate that iAs can impair TLR3 expression and anti-tumor pathway activated by PIC+9cRA in SCs and prostatic epithelial cells. These findings suggest that TLR3-activation based therapy may be an ineffective therapeutic alternative for iAs-associated PCa.
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Células Epiteliais/efeitos dos fármacos , Células Epiteliais/metabolismo , Células-Tronco Neoplásicas/efeitos dos fármacos , Células-Tronco Neoplásicas/metabolismo , Neoplasias da Próstata/genética , Neoplasias da Próstata/metabolismo , Compostos de Sódio/toxicidade , Receptores Toll-Like/efeitos dos fármacos , Adulto , Idoso , Idoso de 80 Anos ou mais , Apoptose/efeitos dos fármacos , Transformação Celular Neoplásica/efeitos dos fármacos , Transformação Celular Neoplásica/metabolismo , Células Cultivadas/efeitos dos fármacos , Células Cultivadas/metabolismo , Variação Genética , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Próstata/efeitos dos fármacos , Próstata/metabolismo , Neoplasias da Próstata/fisiopatologia , Compostos de Sódio/metabolismo , Receptores Toll-Like/metabolismoRESUMO
OBJECTIVES To determine the role of the RBP4/PiC/SIRT3 signaling pathway in the opening of the mitochondria permeability transition pore (mPTP) in offspring rats with hypothyroidism during pregnancy. METHODS Sixty Sprague-Dawley (SD) rats were employed in this study. Pregnancy was deemed successful when a sperm was found in the uterus. After one week of pregnancy, offspring rats were divided into the following groups: overall hypothyroidism group (OH group), subclinical hypothyroidism group (SCH group), and normal control group (CON group). The establishment of the hypothyroidism model was confirmed when the serum thyroid stimulating hormone (TSH) levels were higher than normal value and TT4 level was within the normal range. The renal mitochondria of offspring rats were extracted on the 14th postnatal day (P14) and 35th postnatal day (P35). RESULTS At P14, no significant differences in the degree of mPTP opening and expression of phosphoric acid carrier vector (PiC) were detected between the rats in the OH group and the SCH group. However, the expression level of silent mating-type information regulation 3 homolog (SIRT3) was markedly reduced. Retinol-binding protein 4 (RBP4) expression increased in the rats from the OH group, relative to that in those from the SCH group. At P35, the degree of mPTP opening and the expression levels of PiC and RBP4 in the OH group were higher than those in the SCH group. However, SIRT3 expression in the OH group was lower than that observed in the SCH group. CONCLUSION RBP4 plays an important role in early renal mitochondrial damage and renal impairment in rats suffering from hypothyroidism during pregnancy. The RBP4/PiC/SIRT3 pathway is thus involved in the opening of the renal mPTP in offspring rats with hyperthyroidism.
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Animais , Feminino , Gravidez , Ratos , Complicações na Gravidez , Hipotireoidismo/complicações , Hipotireoidismo/induzido quimicamente , Rim/metabolismo , Rim/patologia , Mitocôndrias , Permeabilidade , Ratos Sprague-Dawley , Proteínas Plasmáticas de Ligação ao RetinolRESUMO
A meditação no Ocidente vem se tornando objeto de estudos e pesquisas nas diversas áreas do campo da saúde, devido às evidencias de que a prática da meditação promove diversos benefícios à saúde física e mental, com reflexos importantes na qualidade de vida e bem-estar dos praticantes. Neste sentido diversos estudos apontam a relevância da meditação na atividade mental, na saúde física e na relação entre corpo e mente, indicando a necessidade da inclusão da prática no cuidado à saúde. Oriundas de diversas partes do mundo as práticas meditativas estão incorporadas há milênios em diversas culturas e tradições. Desse modo, esse estudo objetivou refletir sobre como a meditação e/ou prática meditativa foram assimiladas e são aplicadas em processos grupais no âmbito da SMS/Sistema Único de Saúde do Município de São Paulo. Para isso, foi realizado um estudo quantitativo de caráter exploratório descritivo e reflexivo. O método utilizado foi o de revisão analítica de base bibliográfica e documental, a partir de bancos de dados oficiais de pesquisa (TabNet/DATASUS e SISAB) e literatura científica (artigos, dissertações, livros, revistas científicas eletrônicas). Dos 278 estudos encontrados, após leitura dos títulos e resumos dos artigos, a amostra final totalizou em 14 estudos selecionados. A realização desse estudo permitiu visualizar os estudos da meditação nos grupos coletivos, a importância das políticas públicas e sua eficácia na prevenção e promoção da saúde. Os benefícios da meditação foram categoricamente demonstrados, a diminuição do uso de medicamentos, a sensação de bem-estar, percepção do corpo e melhor compreensão do processo de adoecimento.
Meditation in the West has become an object of studies and research in different areas of the health field, due to the evidence that the practice of meditation promotes several benefits to physical and mental health, with important impacts on the quality of life and well-being. be of the practitioners. In this sense, several studies point out the relevance of meditation in mental activity, in physical health and in the relationship between body and mind, indicating the need to include the practice in health care. Meditative practices originating from different parts ofthe world have been incorporated for millennia in different cultures and traditions. Thus, this study aimed to reflect on how meditation and / or meditative practice were assimilated and are applied in group processes within the scope of the SMS / Unified Health System of the Municipality of São Paulo. For this, a quantitative study of exploratory, descriptive and reflective character was carried out. The method used is an analytical review of bibliographic and documentary basis, from official research databases (TabNet / DATASUS and SISAB) and scientific literature (articles, dissertations, books, electronic scientific journals). Of the 278 studies found, after reading the titles and abstracts of the articles, the final sample totaled 14 selected studies. The realization of this study made it possible to visualize meditation studies in collective groups, the importance of public policies and their effectiveness in preventing and promoting health. The benefits of meditation have been categorically demonstrated, the decrease in the use of medications, the feeling of well-being, perception of the body and a better understanding of the illness process.
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Humanos , Masculino , FemininoRESUMO
Resumo: As práticas Integrativas e complementares têm se incorporado ao sistema de saúde de maneira significativa, na assistência a saúde dos pacientes, especialmente na área da oncologia. O câncer é uma doença devastadora que impacta de maneira negativa na vida do paciente, família e equipe. A utilização das Praticas Integrativas e Complementares (PIC's) são uma alternativa que complementam os tratamentos convencionais na melhora da qualidade de vida e no cuidado a dimensão espiritual do ser humano. Objetivo: Relatar a experiência de implantação das Terapias Integrativas e Complementares através do Projeto de Extensão Luzes. Métodos: as atividades realizadas são Reiki, Auriculoterapia, massagem laboral, meditação, grupo de oração, música e canto, Bio Energetics Medicine, Contação de histórias, confecção de origamis, avaliação da saúde bucal, construção de Mandalas e pinturas. As PIC's são desenvolvidas por um grupo de 136 voluntários dentre docentes, discentes e profissionais de diferentes áreas e da comunidade em geral. Desde sua implantação em Agosto de 2018 já atendeu mais de 1700 pessoas. É desenvolvido dentro do Hospital Regional do Oeste de Chapeco SC nos setores de Oncologia, Quimioterapia e Radioterapia. Resultados: as terapias integrativas e complementares são reconhecidas como benéficas pelos pacientes, familiares e profissionais. Consideradas aliadas na melhora da qualidade de vida durante o período de internação e tratamento. Também citada como uma maneira diferenciada de assistência a dimensão espiritual porque propicia a formação de vinculos mais empáticos entre profissionais, pacientes e família. Observa-se também uma maior qualificação no processo de formação profissional dos acadêmicos dos cursos de Enfermagem e Medicina da UFFS. Conclusões: A utilização das praticas integrativas e complementares no ambiente hospitalar foram sem sombra de dúvidas um desafio. Devemos reconhecer que valeu o desafio de implantar as Terapias Integrativas e Complementares que aliadas ao tratamento convencional trouxeram benefícios impares a tríade paciente, família e profissional. Um espaço de pratica das PIC's possibilita a formação de recursos humanos voltados ao SUS, com uma visão ampliada ao processo saúde-doença e mais competentes e comprometidos com a saúde integral do ser humano.
Integrative and complementary practices have been significantly incorporated into the health system in the health care of patients, especially in the area of oncology. Cancer is a devastating disease that negatively impacts the lives of patients, families and staff. The use of Integrative and Complementary Practices (ICPs) is an alternative that complements conventional treatments in improving the quality of life and caring for the spiritual dimension of the human being. Objective: Report the experience of implementation of Integrative and Complementary Therapies through the Lights Extension Project. Methods: The activities performed are Reiki, Auriculotherapy, Labor Massage, Meditation, Prayer Group, Music and Singing, Bio Energetics Medicine, Storytelling, Origami Making, Oral Health Assessment, Mandala Construction and Painting. PICs are developed by a group of 136 volunteers including teachers, students and professionals from different fields and the wider community. Since its implementation in August 2018, it has served more than 1700 people. It is developed within the Western Regional Hospital of Chapeco SC in the Oncology, Chemotherapy and Radiotherapy sectors. Results: Integrative and complementary therapies are recognized as beneficial by patients, families and professionals. Considered allies in improving the quality of life during hospitalization and treatment. Also cited as a different way of assisting the spiritual dimension because it provides the formation of more empathic links between professionals, patients and family. There is also a higher qualification in the process of professional training of students of Nursing and Medicine courses at UFFS. Conclusions: The use of integrative and complementary practices in the hospital environment was undoubtedly a challenge. We must recognize that the challenge of implementing the Integrative and Complementary Therapies that combined with the conventional treatment brought unfair benefits to the patient, family and professional triad was worth the challenge. A practice space for ICPs enables the formation of human resources focused on the SUS, with a broader view of the health disease process and more competent and committed to the integral health of human beings.
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Resumen El pato criollo peruano (Cairina moschata domestica) es una de las especies de mayor importancia económica en la alimentación humana. Las especies de patos forman grupos genéticos complejos y difíciles de reconocer, por lo que el uso marcadores microsatélites (SSR) identificados en una especie relacionada como Anas platyrhynchos, representa una opción atractiva, de menor costo y útil para resolver temas relacionados con la conservación de la diversidad genómica, flujo génico e hibridación entre poblaciones. El objetivo de la investigación fue evaluar la transferibilidad de 24 SSR identificados para A. platyrhynchos a las poblaciones peruanas de C. moschata doméstica y determinar el grado de polimorfismo (PIC) de los marcadores transferibles. Para ello, se obtuvo ADN a partir de plumas alares usando el método cloroformo-alcohol isoamílico. Los SSR se construyeron con una secuencia adicional de 19 pb (cola M13) y se utilizaron fluoróforos 6-FAM, VIC, NED y PET para su etiquetado. Los fragmentos amplificados fueron visualizados en geles de agarosa 2% y separados por electroforesis capilar en un secuenciador automático ABI 3130XL. Los resultados mostraron 7 SSRs con un valor PIC alto (PIC>0.5) y que el marcador CMO211 se expresaba con un tamaño molecular menor del de la referencia. En conclusión, el presente trabajo demostró que el 75% de los SSR diseñados para A. platyrhynchos son transferibles a C. moschata domestica; y que sólo 7 fueron altamente informativos. Demostrando así que los SSRs son útiles en la detección de polimorfismos en especies relacionadas y pueden ser usados para mejorar las poblaciones peruanas de patos criollos.
Abstract Peruvian Muskovy duck (Cairina moschata domestica) is one of the most economically important species in human nutrition. Duck species form complex genetic groups which are difficult to recognize, thus the use microsatellite markers (SSRs) identified already in Anas platyrhynchos (related species), represents a very attractive option for its cheapness and usefulness for solving issues related to conservation of genomic diversity, gene flow and hybridization between population. The main goal of this work was to evaluate the degree of polymorphism (PIC) and the transferability of 24 SSRs identified for A. platyrhynchos to C. moschata domestica. In this study, DNA collected from wing feathers was extracted using the chloroform-isoamyl alcohol method. SSRs were constructed with an additional 19 bp sequence (M13 tail) and 6-FAM, VIC, NED and PET fluorophores were used for their labeling. The amplified fragments were visualized on 2% agarose gels and separated by capillary electrophoresis in an automatic ABI 3130XL sequencer. Results showed 7 SSR with high PIC value (PIC> 0.5) and the CMO211 marker expressed in a smaller molecular size that the one used as reference. In conclusion, we showed that 75% of the SSR designed for A. platyrhynchos were transferable to C. moschata domestica as well as we found only 7 SSR highly informative, thus we proved that SSR are highly useful for detecting polymorphisms in related species and improved the Peruvian populations of Muskovy ducks.
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Escherichia coli is an important pathogen responsible for a variety of diseases. We have recently shown that Pic, a serine protease secreted by E. coli, mediates immune evasion by the direct cleavage of complement molecules. The aim of this study was to investigate the action of a Pic-producing bacteria in a murine model of sepsis. Mice were infected with Pic-producing E. coli (F5) or F5∆pic mutant. Animal survival was monitored for five days, and a subset of mice was euthanized after 12 h for sample acquisition. The inoculation of Pic-producing bacteria induced 100% death within 24 h. The colony forming units count in the organs was significantly higher in F5. Hematological analysis showed a decrease of total leukocytes. Nitric oxide and cytokines were detected in serum, as well as on peritoneal lavage of the F5 group in higher levels than those detected in the other groups. In addition, immunophenotyping showed a decrease of activated lymphocytes and macrophages in the F5 group. Therefore, Pic represents an important virulence factor, allowing the survival of the bacterium in the bloodstream and several organs, as well as inducing a high production of proinflammatory mediators by the host, and concomitantly a cellular immunosuppression, leading to sepsis and death.
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Citocinas/metabolismo , Infecções por Escherichia coli/metabolismo , Proteínas de Escherichia coli/metabolismo , Escherichia coli/metabolismo , Sepse/metabolismo , Serina Endopeptidases/metabolismo , Animais , Citocinas/genética , Escherichia coli/genética , Escherichia coli/patogenicidade , Infecções por Escherichia coli/genética , Infecções por Escherichia coli/microbiologia , Infecções por Escherichia coli/patologia , Proteínas de Escherichia coli/genética , Feminino , Inflamação/genética , Inflamação/metabolismo , Inflamação/microbiologia , Inflamação/patologia , Camundongos , Sepse/genética , Sepse/microbiologia , Sepse/patologia , Serina Endopeptidases/genéticaRESUMO
This review was carried out with the purpose of contributing to the discussion on the equations used in calculating the Polymorphism Information Content (PIC) of molecular markers. PIC measures the ability of a marker to detect polymorphisms, and therefore has enormous importance in selecting markers for genetic studies. We perform a summary analysis of PIC and its difference in relation to heterozygosity, another parameter used to evaluate the quality of a marker, presenting and discussing the several equations registered in the literature for both dominant and codominant markers. Finally, we present a brief direction on estimating PIC for dominant markers.