RESUMO
The experiment was carried out to evaluate the quality of anhydrous milk fat (AMF) of cows and buffaloes supplemented with flaxseed oil (FO), soybean oil (SO), or their mixture (FSO). Lactating crossbred cows and buffaloes were fed with control diet or with one of three supplements: 2% FO, 2% SO, and 2% FSO according to a double 4 x4 Latin Square Design. The diets with FO, SO, or FSO reduced saturated FA, mainly C4:0, C14:0 and C16:0, while increased the unsaturated FA C18:1 and C18:2 in milk from cows andbuffaloes. Cholesterol content decreased in cow's AMF while increased in buffalo's AMF when a diet supplemented with FO, SO, or FSO. The diet with SO or FSO increased the content of vitamin E in AMF obtained from cows (25.06 and 17.89 mg 100 g-1) and buffaloes (28.48 and 30.32 mg 100 g-1) compared with the control diet (11.02 and 15.68 mg 100 g-1), respectively, which correlated positively with scavenging activity for DPPH⢠(r2= 0.66) and ABTSâ¢(r2= 0.67) radicals. Solid fat content (SFC) was high for cow's AMF, with 58.12-60.37% at 5°C compared to that of buffalo's AMF, with 52.37-56.98%, but was low for cow's AMF at >15°C. Finally, supplementing a diet with vegetable oils, particularly SO, improves the quality of AMF; increases USFA/SFA ratio, vitamin E content, and antioxidant activities.(AU)
Assuntos
Animais , Feminino , Suplementos Nutricionais/análise , Leite/química , Ingestão de Alimentos/fisiologia , Glycine max/fisiologia , Óleo de Semente do Linho , Búfalos/fisiologia , Bovinos/fisiologiaRESUMO
This study aimed the enzymatic decyl esters production by hydroesterification, a two-step process consisting of hydrolysis of refined soybean (RSBO) or used soybean cooking (USCO) oils to produce free fatty acids (FFA) and further esterification of purified FFA. Using free lipase from Candida rugosa (CRL), about 98% hydrolyses for both oils have been observed after 180 min of reaction using a CRL loading of 50 U g-1 of reaction mixture, 40 °C, and a mechanical stirring of 1500 rpm. FFA esterification with decanol in solvent-free systems was performed using lipase from Thermomyces lanuginosus (TLL) immobilized by physical adsorption on silica particles extracted from rice husk, an agricultural waste. For such purpose, non-functionalized (SiO2) or functionalized rice husk silica bearing octyl (Octyl-SiO2) or phenyl (Phe-SiO2) groups have been used as immobilization supports. Protein amounts between 22 and 28 mg g-1 of support were observed. When used in the esterification, they enabled a FFA conversion of 81.3-87.6% after 90-300 min of reaction. Lipozyme TL IM, a commercial immobilized TLL, exhibited similar performance compared to TLL-Octyl-SiO2 (FFA conversion ≈90% after 90-120 min of reaction). However, high operational stability after fifteen successive esterification batches was observed only for TLL immobilized on Octyl-SiO2 (activity retention of ≈90% using both FFA sources). The produced decyl esters presented good characteristics as potential biolubricants according to standard methods (ASTM) and thermal analysis.