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El control del comportamiento de la percepción de cansancio a lo largo del macrociclo de entrenamiento en decatlonistas constituye un indicador para la valoración del proceso de asimilación psicofísica del trabajo realizado acorde a la etapa del macrociclo de entrenamiento por la que transita el atleta. Sin embargo, el insuficiente conocimiento sobre el tema en el decatlón ha limitado el uso de este indicador en el control psicológico de la preparación en este deporte. El objetivo de este trabajo consiste en determinar el comportamiento de la percepción de cansancio en las diferentes etapas del macrociclo de entrenamiento, en decatlonistas de la selección nacional de atletismo de Cuba. Es un estudio descriptivo, en el que se aplica la entrevista para un diagnóstico inicial y la Escala de Borg (modificada), para la medición de la percepción de cansancio. Fueron realizadas 416 mediciones de la percepción de cansancio, durante las etapas de preparación física, general, especial y competitiva. Como principales resultados, la percepción de cansancio aumentó en la etapa de preparación física general en una magnitud de 3.48, en la etapa especial en un 3.43 y en la competitiva en un 2.56. La percepción de cansancio en los decatlonistas estudiados aumentó, significativamente, posterior a las cargas de entrenamiento en cada una de las etapas estudiadas y disminuyó, a medida que avanzó el macrociclo de entrenamiento. La etapa competitiva resultó ser aquella de menor cansancio percibido pre- y poscarga, y la de preparación física general, la de mayor aumento en este proceso.

O controle do comportamento da percepção da fadiga ao longo do macrociclo de treinamento em decatletas constitui um indicador para a avaliação do processo de assimilação psicofísica do trabalho realizado de acordo com a etapa do macrociclo de treinamento pela qual o atleta passa. Contudo, o conhecimento insuficiente sobre o tema no decatlo tem limitado a utilização deste indicador no controle psicológico da preparação neste esporte. O objetivo deste trabalho é determinar o comportamento da percepção da fadiga nas diferentes etapas do macrociclo de treinamento, em decatlonistas da seleção cubana de atletismo. Trata-se de um estudo descritivo, no qual é aplicada a entrevista para diagnóstico inicial e a Escala de Borg (modificada) para mensurar a percepção de fadiga. Foram realizadas 416 medidas de percepção de fadiga durante as etapas de preparação física, geral, especial e competitiva. Como principais resultados, a percepção de fadiga aumentou na etapa de preparação física geral em uma magnitude de 3,48, na etapa especial em 3,43 e na etapa competitiva em 2,56. A percepção de fadiga no decatleta estudado aumentou significativamente após as cargas de treinamento em cada uma das etapas estudadas e diminuiu à medida que o macrociclo de treinamento avançava. A etapa competitiva acabou sendo a que apresentou menor fadiga percebida pré e pós-carga, e a etapa de preparação física geral, a que apresentou maior aumento nesse processo.

The control of the behavior of the perception of tiredness throughout the training macrocycle in decathletes constitutes an indicator for the assessment of the process of psychophysical assimilation of the work carried out according to the stage of the training macrocycle through which the athlete goes. However, insufficient knowledge on the subject in the decathlon has limited the use of this indicator in the psychological control of preparation in this sport. The objective of this work is to determine the behavior of the perception of tiredness in the different stages of the training macrocycle, in tiredness of the Cuban national athletics team. It is a descriptive study, in which the interview is applied for an initial diagnosis and the Borg Scale (modified) to measure the perception of tiredness. 416 measurements of the perception of tiredness were carried out during the stages of general, special and competitive physical preparation. As the main results, the perception of tiredness increased in the general physical preparation stage by a magnitude of 3.48, in the special stage by 3.43 and in the competitive stage by 2.56. The perception of tiredness in the decathletes studied increased significantly after the training loads in each of the stages studied and decreased as the training macrocycle progressed. The competitive stage turned out to be the one with the least perceived pre- and post-load tiredness, and the general physical preparation stage was the one with the greatest increase in this process.

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OBJECTIVE: This study aims to evaluate the relationship between Total Body Score (TBS) and Accumulated Degree-Days (ADD) for estimating postmortem interval (PMI) using the decomposition quantification system by Megyesi et al. (Megyesi MS, Nawrocki SP, Haskell NH (2005) Using Accumulated Degree-Days to Estimate the Postmortem Interval from Decomposed Human Remains. J Forensic Sci 50:1-9. https://doi.org/10.1520/jfs2004017 ). DESIGN: A systematic review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. The methodological quality and risk of bias were assessed using the QUADAS-2 scoring system. Statical tests, including I2 for heterogeneity assessment and subgroup analysis comparing human and pig proxies across different decomposition stages, were performed for meta-analysis. RESULTS: The search identified 25 studies that underwent qualitative evaluation, all of which were included for quantitative analysis. The findings indicate that the TBS formula tends to overestimate ADD with a moderate mean difference of 0.5758 overall. Specifically, in pigs, ADD is overestimated significantly (1.1128), while there is a slight underestimation in humans (-0.0038). Across decomposition stages, fresh body (0.0066) and early decomposition (0.0338) show an insignificant overestimation, whereas advanced decomposition reveals a slight underestimation (-0.3378) and skeletonization indicates a substantial overestimation (1.6583). CONCLUSIONS: The relationship between TBS and ADD demonstrates high accuracy in humans during early decomposition stages, without differences in statistical significance. However, its accuracy diminishes as decomposition progresses, potentially leading to an overestimation of PMI.

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BACKGROUND: Sleep-related bruxism (SB) is the habit of grinding or clenching the teeth during sleep, mediated by the non-peripheral central nervous system. PURPOSE: The objectives of this cross-sectional study were to evaluate associations between SB, microarousals and oxyhaemoglobin desaturations and to compare the frequency of SB and microarousals in sleep stages, in an apnoeic population. METHODS: Two hundred and forty individuals composed the sample, who underwent a single full-night polysomnography. Self-reports and clinical inspections were not considered for assessing SB. The polysomnographic assessment of SB was performed using electrodes placed on masseter muscles and chin. SB was defined as more than two events of rhythmic masticatory muscle activity per hour of sleep. Microarousals were considered when there were abrupt changes in electroencephalogram frequencies, without complete awakening, lasting from 3 to 15 s. Oxyhaemoglobin desaturations were defined as significant drops (≥3%) in basal oxygen saturations. With these data, SB, microarousals and oxyhaemoglobin desaturations were evaluated and submitted to statistical analysis. RESULTS: Statistically significant differences were observed between bruxers and non-bruxers when comparing the rates of microarousals (p < .001) and oxyhaemoglobin desaturations (p = .038). There was a higher number of SB and microarousals in NREM (non-rapid eye movement) two sleep stage (p < 0.001). Bruxers had a greater risk of higher numbers of microarousals (OR = 1.023; p = .003), which did not occur for oxyhaemoglobin desaturations (OR = 0.998; p = .741). CONCLUSIONS: A higher number of microarousals presents relationship with SB; associations between SB and oxyhaemoglobin desaturations remained inconclusive; higher frequency of SB and microarousals was observed in NREM 2 sleep stage.

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Early or late pubertal onset can lead to disease in adulthood, including cancer, obesity, type 2 diabetes, metabolic disorders, bone fractures, and psychopathologies. Thus, knowing the age at which puberty is attained is crucial as it can serve as a risk factor for future diseases. Pubertal development is divided into five stages of sexual maturation in boys and girls according to the standardized Tanner scale. We performed genome-wide association studies (GWAS) on the "Growth and Obesity Chilean Cohort Study" cohort composed of admixed children with mainly European and Native American ancestry. Using joint models that integrate time-to-event data with longitudinal trajectories of body mass index (BMI), we identified genetic variants associated with phenotypic transitions between pairs of Tanner stages. We identified $42$ novel significant associations, most of them in boys. The GWAS on Tanner $3\rightarrow 4$ transition in boys captured an association peak around the growth-related genes LARS2 and LIMD1 genes, the former of which causes ovarian dysfunction when mutated. The associated variants are expression and splicing Quantitative Trait Loci regulating gene expression and alternative splicing in multiple tissues. Further, higher individual Native American genetic ancestry proportions predicted a significantly earlier puberty onset in boys but not in girls. Finally, the joint models identified a longitudinal BMI parameter significantly associated with several Tanner stages' transitions, confirming the association of BMI with pubertal timing.

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Determining the moment for harvesting the tropical peanut with a focus on superior seed quality is not an easy task. Particularities such as indeterminate flowering, underground fruiting and uneven maturation further increase this technical challenge. It is in this context that we aim to investigate harvest indicators based on the maturation and late maturation phases of tropical peanuts to obtain seeds with superior physiological and health quality. The plants were grown in field conditions and their development stages were carefully monitored until seed production. The water content, dry weight, germination capacity, desiccation tolerance, vigor, longevity, and seed pathogens were evaluated throughout these stages. We showed that seeds from early stages (R5 and R6) did not fully tolerate desiccation and were highly sensitive to pathogen contamination after storage (Aspergillus, Penicillium, and Bacteria). At late stages (R7, R8, and R9), the seeds had optimized vigor, longevity and bioprotection against fungi and thermal stress. The peanut maturation scale for tropical agriculture provides unique harvesting guidelines that make it possible to monitor the plants' development stages with a focus on producing superior quality seeds.

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Banana is one of the most consumed and popular fruits in all regions of the world, being cultivated mainly in tropical countries. It is not only a rich source of vitamins A, C, and B, calcium, iron, potassium, phosphorus, and other vitamins and nutrients, but it also contains several types of antioxidants with high nutritional value. In this context, the current study aimed to quantify the content of ascorbic acid, flavonoids, pigments, and minerals present in "Nanicão" bananas during the ripening process. As demonstrated, the level of flavonoids was higher in ripe and overripe fruits, whereas the mineral composition was high only at ripening stage 4 (more yellow than green) a stage that should be prioritized when recommending fruit consumption to the population deficient in these minerals. Regarding pigments, there was a reduction in chlorophylls a and b and an increase in carotenoids and anthocyanins in peels and pulps. PRACTICAL APPLICATION: Flavonoids are phenolic, bioactive compounds with proven antioxidant and anti-inflammatory activity and products of the plant's secondary metabolism. The degradation of chlorophylls and synthesis of carotenoids and anthocyanins, and as a consequence of the latter pigment, the increase in flavonoids in the pulp was evident during the monitoring of ripening, mainly in the fruit peels in relation to pigments. Minerals are essential elements, the main ones provided in balanced diets and important for dietary and nutritional health.

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SUMMARY: Calcium-activated chloride channel regulator 1 (CLCA1) is associated with cancer progression. The expression and immunologic function of CLCA1 in stomach adenocarcinoma (STAD) remain unclear. In this investigation, the expression of CLCA1 in STAD tissues and its involvement in the progression and immune response of STAD were examined using databases such as cBioPortal, TISIDB, and UALCAN. In order to validate the expression level of CLCA1 protein in gastric adenocarcinoma, thirty clinical tissue specimens were gathered for immunohistochemical staining. The findings indicated a downregulation of CLCA1 in STAD patients, which was correlated with race, age, cancer grade, Helicobacter pylori infection, and molecular subtype. Through the examination of survival analysis, it was identified that diminished levels of CLCA1 within gastric cancer cases were linked to decreased periods of post-progression survival (PPS), overall survival (OS), and first progression (FP) (P<0.05). The CLCA1 mutation rate was lower in STAD, but the survival rate was higher in the variant group. The correlation between the expression level of CLCA1 and the levels of immune infiltrating cells in STAD, as well as the immune activating molecules, immunosuppressive molecules, MHC molecules, chemokines, and their receptor molecules, was observed. Gene enrichment analysis revealed that CLCA1 may be involved in STAD progression through systemic lupus erythematosus (SLE), proteasome, cell cycle, pancreatic secretion, and PPAR signaling pathways. In summary, CLCA1 is anticipated to function as a prognostic marker for patients with STAD and is linked to the immunization of STAD.

El regulador 1 del canal de cloruro activado por calcio (CLCA1) está asociado con la progresión del cáncer. La expresión y la función inmunológica de CLCA1 en el adenocarcinoma de estómago (STAD) aún no están claras. En esta investigación, se examinó la expresión de CLCA1 en tejidos STAD y su participación en la progresión y respuesta inmune de STAD utilizando bases de datos como cBioPortal, TISIDB y UALCAN. Para validar el nivel de expresión de la proteína CLCA1 en el adenocarcinoma gástrico, se recolectaron treinta muestras de tejido clínico para tinción inmunohistoquímica. Los hallazgos indicaron una regulación negativa de CLCA1 en pacientes con STAD, que se correlacionó con la raza, la edad, el grado del cáncer, la infección por Helicobacter pylori y el subtipo molecular. Mediante el examen del análisis de supervivencia, se identificó que los niveles reducidos de CLCA1 en los casos de cáncer gástrico estaban relacionados con períodos reducidos de supervivencia posterior a la progresión (PPS), supervivencia general (OS) y primera progresión (FP) (P <0,05). La tasa de mutación CLCA1 fue menor en STAD, pero la tasa de supervivencia fue mayor en el grupo variante. Se observó la correlación entre el nivel de expresión de CLCA1 y los niveles de células inmunes infiltrantes en STAD, así como las moléculas activadoras inmunes, moléculas inmunosupresoras, moléculas MHC, quimiocinas y sus moléculas receptoras. El análisis de enriquecimiento genético reveló que CLCA1 puede estar involucrado en la progresión de STAD a través del lupus eritematoso sistémico (LES), el proteasoma, el ciclo celular, la secreción pancreática y las vías de señalización de PPAR. En resumen, se prevé que CLCA1 funcione como un marcador de pronóstico para pacientes con STAD y está vinculado a la inmunización de STAD.

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This study delves into the foraging ecology of the cookiecutter shark (Isistius brasiliensis) in the Saint Peter and Saint Paul Archipelago (SPSPA), assessing bite marks on pelagic fishes collected between March and September 2018. Examination of 200 individuals from five species revealed the shark's predilection for targeting adult fish, ensuring efficient removal of energy-rich tissue layers. However, a notable exception is Thunnus albacares, preyed on predominantly during its juvenile stage, possibly due to vertical spatial segregation. Recent bites were pervasive across species, with wahoo displaying a distinctive pattern. Cookiecutter shark bites predominantly targeted the lateral body portion (>55%) except for yellowfin tuna, which exhibited a preference for the ventral region (95%). The estimated length distribution suggests the SPSPA as a potential nursery area for cookiecutter sharks. Bite mark positions and frequencies may be linked to fish behavior, swimming velocity, and fishing gear strategy, highlighting the complex interplay in pelagic ecosystems.

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The age and daily growth of fish are registered through the deposition of increments in their otoliths, which are concretions formed by the precipitation of substances present in the endolymphatic fluid, mainly calcium carbonate (CaCO3). Faced with the need to fill some of the gaps in the knowledge on the occurrence and duration of the initial stages of snapper species' life cycles in the Abrolhos Bank, this study aimed to describe the growth rates, age, and period of pelagic larval duration (PLD) of three snapper species during the larval pre-settlement phase, in the Abrolhos Bank region. The post-larvae were captured using light traps. Otoliths were removed from 117 samples of snapper species; however, only 69 were viable for age estimation, of which 15 were Lutjanus analis, 25 were Lutjanus jocu, and 29 were Lutjanus synagris. Together, the samples presented individuals with total lengths ranging from 16.14 to 24.76 mm and ages from 21 to 39 days. Settlement marks were found for all three species, and the average PLD was ~25 days. The somatic growth of the snapper species was positively correlated with otolith growth. L. jocu presented the greatest daily growth compared to the other species. The three species use the Abrolhos Bank as a larval settlement site, demonstrating plasticity by using different habitats throughout their lives.

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Knowledge on mesoclimatic zonation and microclimatic variations within mountain forest ecosystems is crucial for understanding regional species turnover and effects of climate change on these systems. The temperate mountain forests in the Andean region of South America are among the largest and contiguous natural deciduous forest areas in the world. Due to their pronounced disturbance regime and different successional stages, a climatic zonation combined with the characterisation of its microclimatic variation is important to identify thresholds of species occurrences.We used micro-loggers to measure air temperature and relative humidity for one year at 40 measurement locations along longitudinal and elevation gradients in mountain forests in Northern Patagonia, Argentina. Our results unveil mesoclimatic patterns within these forests characterised by variations in temperature and vapour pressure deficit along the elevational gradient in general, but also at different times of the year. For example, Austrocedrus chilensis and Nothofagus dombeyi forests differed mainly by temperature and its diurnal range in the warmest months of the year. Also, differences between forest stands and gaps were more pronounced in the warmest months of the year and at lower elevations, with up to 2.5 K higher temperatures in the second half of the day in gaps. We found clear indications that shrubland of Nothofagus antarctica representing a successional stage after disturbances alters the mesoclimatic pattern, favouring forest fire ignition. Such mesoclimatic variations have a major influence on tree species turnover and ecological processes within these forest ecosystems.The findings contribute to our understanding of the complex interplay between topography, climate, and vegetation in shaping the spatial patterns of species occurrences.

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