RESUMO

This study aimed to investigate the effects of chronic ß-alanine (ßA) plus acute sodium bicarbonate (SB) co-supplementation on neuromuscular fatigue during high-intensity intermittent efforts in swimming. Eleven regional and national competitive-level young swimmers performed a neuromuscular fatigue assessment before and immediately after two 20 × 25-m front crawl maximal efforts every 90 s, performed at pre- and post-4-week co-supplementation. Neuromuscular fatigue was evaluated by percutaneous electrical stimuli through the twitch interpolation technique on the triceps brachii and quadriceps femoris. Performance was defined by the mean time of the 20 efforts and blood samples to lactate concentrations were collected every four efforts. Participants supplemented 3.2-6.4 g·day-1 of chronic ßA or placebo (PL) during four weeks, and acute 0.3 g·kg-1 of SB or PL 60 min before the second assessment (allowing ßA+SB and PL+PL groups). No statistical changes were found in neuromuscular fatigue of triceps brachii. In the quadriceps femoris, a main effect of time was found in potentiated twitch delta values in pooled groups, showing a statistical increase of 19.01% after four weeks (Δ = 13.05 [0.35-25.75] N; p = 0.044), without time × group interactions. No statistical difference was found in the swimming performance. Blood lactate increased by 25.06% only in the ßA+SB group (Δ = 6.40 [4.62-8.18] mM; p Bonf < 0.001) after the supplementation period. In conclusion, 4-week ßA and SB co-supplementation were not able to reduce neuromuscular fatigue levels and improve performance in highintensity intermittent efforts, but statistically increased blood lactate levels.

RESUMO

Hypersecretion of gastric acid damages the stomach lining, causing the formation of peptic ulcers. Mucilage from medicinal plants offers a relaxing and soothing effect to the endodermal lining of the gut and has antacid properties, which can protect the mucosal lining from gastric acidity. This is the first report aimed to evaluate the physicochemical characteristics, acid-neutralizing, and cytotoxicity properties of traditionally used aqueous mucilage from Asparagus exuvialis and Sesamum capense. The physicochemical properties were determined by biochemical methods. Acid neutralizing and buffering capacities were determined by titration methods. Normal mouse embryonic fibroblast cells were used for cytotoxicity evaluation by MTT assay. The physicochemical characterization confirmed the presence of carbohydrates, alkaloids, saponins, proteins, tannins, flavonoids, and glycosides. Sesamum capense mucilage exhibited the most potent artificial gastric juice neutralizing capacity pH of 4.62±0.01, 8.0±0.00 acid neutralization capacity per gram of acid, and 30 minutes duration of acid neutralization. The aqueous mucilage from S. capense did not cause any significant cytotoxicity to 3T3 cell lines showing an IC50 value of 91.5 ± 0.06 µg mL-1, confirming the safe nature of the mucilage. These findings revealed that S. capense has the potential to neutralize gastric acid responsible for ulceration and can be safely consumed.(AU)

Assuntos

RESUMO

The aim of this study was to evaluate the effect of inclusion of two acidifiers blend (with different protection levels) on the acidification potential in vitro, apparent total tract digestibility of diet nutrients, blood metabolites, and average daily gain of nursery piglets. Two commercial acidifiers blend additives (AC1 and AC2) were evaluated. The acidification potential of the two acidifiers blend added to the diet were tested by buffering capacity and initial pH in vitro. For the evaluation of apparent digestibility of diet nutrients, blood metabolites and average daily gain, two experiments were carried out. The AC2 acidifier had a greater effect on the initial reduction in pH and a lower linear buffering rate of the diet. The use of acidifiers did not improve the nutrient digestibility of the diets and had no effect on the blood metabolite concentrations of urea, total protein, albumin, globulin, and lactate. Although the inclusion of acidifiers in the diets did not affect the apparent total tract digestibility of the diet nutrients the inclusion of the AC2 acidifier at different levels showed a negative quadratic effect on the average daily gain, with the best inclusion level being 0.26%.

O objetivo deste estudo foi avaliar o efeito da inclusão de dois acidificantes (com diferentes níveis de proteção) sobre o potencial de acidificação in vitro, a digestibilidade aparente do trato total dos nutrientes da dieta, os metabólitos sanguíneos e o ganho médio diário de leitões. Foram avaliados dois acidificantes comerciais (AC1 e AC2). O potencial de acidificação dos acidificantes adicionados às dietas foi testado quanto à capacidade tamponante e ao pH inicial in vitro. Para avaliação da digestibilidade aparente dos nutrientes da dieta, dos metabólitos sanguíneos e do ganho médio diário, foram realizados dois experimentos. O acidificante AC2 teve maior efeito na redução inicial do pH e menor taxa linear de tamponamento linear da dieta. O uso de acidificantes não melhorou a digestibilidade dos nutrientes das dietas e não teve efeito sobre as concentrações dos metabólitos sanguíneos de ureia, proteína total, albumina, globulina e lactato. Embora a inclusão de acidificantes nas dietas não tenha afetado a digestibilidade aparente do trato total dos nutrientes da dieta, a inclusão do acidificante AC2 em diferentes níveis apresentou efeito quadrático negativo no ganho médio diário, com o melhor nível de inclusão sendo 0,26%.

Assuntos

RESUMO

In the present study, the modification of branched polyethyleneimine (b-PEI) was carried out using mesquite gum (MG) to improve its hemocompatibility to be used in biomedical applications. In the copolymer synthesis process (carboxymethylated mesquite gum grafted polyethyleneimine copolymer (CBX-MG-PEI), an MG carboxymethylation reaction was initially carried out (carboxymethylated mesquite gum (CBX-MG). Subsequently, the functionalization between CBX-MG and b-PEI was carried out using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as crosslinking agents. The synthesis products were characterized using Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and thermogravimetric analysis (TGA). Thermogravimetric analysis showed that CBX-MG and CBX-MG-PEI presented a lower decomposition temperature than MG. The CBX-MG-PEI has a high buffer capacity in the pH range of 4 to 7, similar to the b-PEI. In addition, the CBX-MG-PEI showed an improvement in hemocompatibility in comparison with the b-PEI. The results showed a non-hemolytic property at doses lower than 0.1 µg/mL (CBX-MG-PEI). These results allow us to propose that this copolymer be used in transfection, polymeric nanoparticles, and biomaterials due to its physicochemical and hemocompatibility properties.

RESUMO

Ensiling sorghum with tropical forages has been shown to be a promising alternative for ruminant feed production, as this approach improves the quality of the sorghum silage. In this context, the goal of this study was to evaluate the fermentation characteristics and nutritive value of sweet sorghum silage made with different levels of Paiaguas palisadegrass and Ipypora grass. The experiment was conducted in a completely randomized 2 x 6 factorial design with four replications. Two forages (Paiaguas palisadegrass and Ipypora grass) were added to sorghum at six levels (0, 20, 40, 60, 80 and 100%), for a total of 48 experimental silos. After 50 days of fermentation, the silos were opened, and the fermentation profile and chemical composition of the silage were analysed. The results showed that the addition of Paiaguas palisade grass and Ipyporagrass to the sorghum silage increased the pH, buffering capacity and NH3-NT of the silage and reduced its dry matter content but did not affect its fermentation characteristics, which remained within an appropriate range. The addition of increasing levels of Paiaguas palisadegrass and Ipypora grass to the sorghum silage increased the crude protein and in vitro dry matter digestibility (IVDMD) of the silage and decreased the fibre fraction (neutral detergent fibre, acid detergent fibre and lignin) and ether extract contents. Adding tropical forages to sorghum silage material at levels above 40% is recommended. Among forage crops, addition of Paiaguas palisadegrass to sorghum silage resulted in higher content of crude protein and IVDMD. Mixed silages can be an effective alternative to improve the quality of forage sorghum silage.

A cultura do sorgo ensilado com forrageiras tropicais, vem se mostrando alternativa promissora de alimentação para a produção de ruminantes, por proporcionar melhorias na qualidade da silagem. Neste contexto, objetivou-se avaliar as características fermentativa e valor nutritivo da silagem de sorgo sacarino com níveis de capins Paiaguás e Ipyporã. O experimento foi conduzido no delineamento experimental inteiramente casualizado, com quatro repetições, em esquema fatorial 2 x 6, sendo duas forrageiras (Paiaguás e Ipyporã) adicionados ao sorgo em seis níveis (0; 20; 40; 60, 80 e 100%), totalizando 48 silos experimentais. Após 50 dias de fermentação, os silos foram abertos para realização das análises do perfil fermentativo e composição bromatológica. Os resultados mostraram que a adição dos capins Paiaguás e Ipyporã na ensilagem de sorgo aumentou o pH, capacidade tampão e N-NH3 e, reduziu os teores de matéria seca, mas não comprometeu as características fermentativas das silagens, ficando dentro do padrão adequado. A adição de níveis crescente de capins Paiaguás e Ipyporã na ensilagem de sorgo aumentou os teores de proteína bruta e digestibilidade in vitro da matéria seca (DIVMS) e diminuiu as frações fibrosas (fibra em detergente neutro, fibra em detergente ácido e lignina) e extrato etéreo, sendo recomendando níveis cima de 40%. Entre as forrageiras, a adição de capim-paiaguás na ensilagem de sorgo apresentou maior teor de proteína bruta e DIVMS. Silagens mistas podem ser uma alternativa eficaz para melhorar a qualidade da silagem exclusiva de sorgo sacarino.

Assuntos

RESUMO

Ensiling sorghum with tropical forages has been shown to be a promising alternative for ruminant feed production, as this approach improves the quality of the sorghum silage. In this context, the goal of this study was to evaluate the fermentation characteristics and nutritive value of sweet sorghum silage made with different levels of Paiaguas palisadegrass and Ipypora grass. The experiment was conducted in a completely randomized 2 x 6 factorial design with four replications. Two forages (Paiaguas palisadegrass and Ipypora grass) were added to sorghum at six levels (0, 20, 40, 60, 80 and 100%), for a total of 48 experimental silos. After 50 days of fermentation, the silos were opened, and the fermentation profile and chemical composition of the silage were analysed. The results showed that the addition of Paiaguas palisade grass and Ipyporagrass to the sorghum silage increased the pH, buffering capacity and NH3-NT of the silage and reduced its dry matter content but did not affect its fermentation characteristics, which remained within an appropriate range. The addition of increasing levels of Paiaguas palisadegrass and Ipypora grass to the sorghum silage increased the crude protein and in vitro dry matter digestibility (IVDMD) of the silage and decreased the fibre fraction (neutral detergent fibre, acid detergent fibre and lignin) and ether extract contents. Adding tropical forages to sorghum silage material at levels above 40% is recommended. Among forage crops, addition of Paiaguas palisadegrass to sorghum silage resulted in higher content of crude protein and IVDMD. Mixed silages can be an effective alternative to improve the quality of forage sorghum silage.(AU)

A cultura do sorgo ensilado com forrageiras tropicais, vem se mostrando alternativa promissora de alimentação para a produção de ruminantes, por proporcionar melhorias na qualidade da silagem. Neste contexto, objetivou-se avaliar as características fermentativa e valor nutritivo da silagem de sorgo sacarino com níveis de capins Paiaguás e Ipyporã. O experimento foi conduzido no delineamento experimental inteiramente casualizado, com quatro repetições, em esquema fatorial 2 x 6, sendo duas forrageiras (Paiaguás e Ipyporã) adicionados ao sorgo em seis níveis (0; 20; 40; 60, 80 e 100%), totalizando 48 silos experimentais. Após 50 dias de fermentação, os silos foram abertos para realização das análises do perfil fermentativo e composição bromatológica. Os resultados mostraram que a adição dos capins Paiaguás e Ipyporã na ensilagem de sorgo aumentou o pH, capacidade tampão e N-NH3 e, reduziu os teores de matéria seca, mas não comprometeu as características fermentativas das silagens, ficando dentro do padrão adequado. A adição de níveis crescente de capins Paiaguás e Ipyporã na ensilagem de sorgo aumentou os teores de proteína bruta e digestibilidade in vitro da matéria seca (DIVMS) e diminuiu as frações fibrosas (fibra em detergente neutro, fibra em detergente ácido e lignina) e extrato etéreo, sendo recomendando níveis cima de 40%. Entre as forrageiras, a adição de capim-paiaguás na ensilagem de sorgo apresentou maior teor de proteína bruta e DIVMS. Silagens mistas podem ser uma alternativa eficaz para melhorar a qualidade da silagem exclusiva de sorgo sacarino.(AU)

Assuntos

RESUMO

The study investigated the influence of ß-alanine supplementation during a high-intensity interval training (HIIT) program on repeated sprint ability (RSA) performance. This study was randomized, double-blinded, and placebo controlled. Eighteen men performed an incremental running test until exhaustion (TINC) at baseline and followed by 4-wk HIIT (10 × 1-min runs 90% maximal TINC velocity [1-min recovery]). Then, participants were randomized into two groups and performed a 6-wk HIIT associated with supplementation of 6.4 g/day of ß-alanine (Gß) or dextrose (placebo group; GP). Pre- and post-6-wk HIIT + supplementation, participants performed the following tests: 1) TINC; 2) supramaximal running test; and 3) 2 × 6 × 35-m sprints (RSA). Before and immediately after RSA, neuromuscular function was assessed by vertical jumps, maximal isometric voluntary contractions of knee extension, and neuromuscular electrical stimulations. Muscle biopsies were performed to determine muscle carnosine content, muscle buffering capacity in vitro (ßmin vitro), and content of phosphofructokinase (PFK), monocarboxylate transporter 4 (MCT4), and hypoxia-inducible factor-1α (HIF-1α). Both groups showed a significant time effect for maximal oxygen uptake (Gß: 6.2 ± 3.6% and GP: 6.5 ± 4.2%; P > 0.01); only Gß showed a time effect for total (-3.0 ± 2.0%; P = 0.001) and best (-3.3 ± 3.0%; P = 0.03) RSA times. A group-by-time interaction was shown after HIIT + Supplementation for muscle carnosine (Gß: 34.4 ± 2.3 mmol·kg-1·dm-1 and GP: 20.7 ± 3.0 mmol·kg-1·dm-1; P = 0.003) and neuromuscular voluntary activation after RSA (Gß: 87.2 ± 3.3% and GP: 78.9 ± 12.4%; P = 0.02). No time effect or group-by-time interaction was shown for supramaximal running test performance, ßm, and content of PFK, MCT4, and HIF-1α. In summary, ß-alanine supplementation during HIIT increased muscle carnosine and attenuated neuromuscular fatigue, which may contribute to an enhancement of RSA performance.NEW & NOTEWORTHY ß-Alanine supplementation during a high-intensity interval training program increased repeated sprint performance. The improvement of muscle carnosine content induced by ß-alanine supplementation may have contributed to an attenuation of central fatigue during repeated sprint. Overall, ß-alanine supplementation may be a useful dietary intervention to prevent fatigue.

Assuntos

RESUMO

Hydrological and hydrochemical processes occurring within riparian zones in temperate mid-latitudes flatland areas have significant implications for water management by controlling nutrient transfer between the watershed and the stream system. The riparian zone in a high-order flatland stream located within a 7063-km2 agricultural watershed in Argentina was investigated to study its hydrological connectivity to upland zones, interactions with the stream, and their implications for groundwater hydrochemistry. The analysis was based on 9-year-long time series of groundwater/stream water levels collected along a 220-m-long transect comprising six piezometers, a river stage sensor, and hydrochemical information from 37 groundwater/stream water sampling campaigns. Samples were analyzed for electrical conductivity (EC), Cl-, SO4+2, (Ca+2 + Mg+2), pH, and redox potential (ORP). Data were interpreted using descriptive statistics, statistical tests, groundwater flux calculations, and identification of hydrological patterns and associated hydrochemical responses. The system was hydrologically controlled by shallow groundwater. Three representative landscape hydrological patterns were identified: disconnected, incipient-weakly connected, and fully connected. Groundwater hydrochemistry was closely linked to hydrological connectivity, which played an important role in the mobilization and fluxes of solutes. Overall, groundwater EC, Cl-, SO4+2, and (Ca+2 + Mg+2) concentrations decreased from upland to lowland. For full connectivity, Cl- concentrations reduced 33%, while SO4+2 reduced 42%, demonstrating the system's buffering capacity. This investigation constitutes the first attempt to formulate the riparian zone functioning in this agricultural region and has contributed to the understanding on the complex interactions between hydrologic regimes of large flatland-high-order streams and shallow groundwater systems in fine-texture sediments.

Assuntos

RESUMO

ABSTRACT Humic acid (HA) may reduce adsorption and increase soil P availability, however, the magnitude of this effect is different when Ca2+ prevails over Mg2+ in limed soils. The objective of this study was to evaluate the effects of HA rates and carbonate sources on the adsorption, phosphate maximum buffering capacity (PMBC), and P availability in two contrasting soils. Oxisol and Entisol samples were firstly incubated with the following HA rates: 0, 20, 50, 100, 200 and 400 mg kg-1, combined with CaCO3 or MgCO3, to evaluate P adsorption. In sequence, soil samples were newly incubated with P (400 mg kg-1) to evaluate P availability. The least P adsorption was found when 296 mg kg-1 of HA was added to Oxisol. Applying HA rates decreased maximum adsorption capacity, increased P binding energy to soil colloids and did not alter PMBC of Entisol. Available P contents in Oxisol increased with HA rates, but it did not change in Entisol. Choosing the right HA rate can decrease PMBC up to 40% and increase the Oxisol P availability by 17%. Application of MgCO3 instead of CaCO3 decreased P adsorption in both soils. Thus, a positive correlation between Ca2+ content and PMBC was verified. Optimum rate of HA and the preponderance of Mg2+ instead of Ca2+ in soil volume fertilized with P are effective practices to reduce adsorption and increase P availability, especially in clayey Oxisol.

RESUMO O ácido húmico (AH) pode reduzir a adsorção e aumentar a disponibilidade de P nos solos, entretanto, a magnitude desse efeito é diferente quando o Ca2+ prevalece sobre Mg2+ em solos com acidez corrigida. Objetivou-se avaliar os efeitos de doses de AH e fontes de carbonato na adsorção, fator capacidade de P máximo (FCPm) e disponibilidade de P em solos contrastantes. Amostras de Latossolo e Gleissolo foram primeiramente incubadas com as seguintes doses de AH: 0, 20, 50, 100, 200 e 400 mg kg-1, combinadas com CaCO3 ou MgCO3, para avaliar a adsorção de P. Em sequência, as amostras de solo foram novamente incubadas com P (400 mg kg-1), para determinar a disponibilidade de P. A aplicação de doses de AH reduziu a capacidade máxima de adsorção, aumentou a energia de ligação do P e não alterou a FCPm do Gleissolo. O teor de P disponível aumentou com o acréscimo das doses de AH no Latossolo, contudo, não foram alterados no Gleissolo. A escolha correta da dose de AH reduziu a FCPm em até 40% e aumentou a disponibilidade de P no Latossolo em 17%. A aplicação de MgCO3 em vez do CaCO3 reduziu a adsorção de P em ambos os solos. Assim, houve correlação positiva entre o teor de Ca2+ e a FCPm. A dose ótima de AH e a predominância de Mg2+ sobre Ca2+ no volume de solo adubado com P são práticas efetivas para reduzir a adsorção e aumentar a disponibilidade de P, especialmente, no Latossolo argiloso.

RESUMO

Since there is conflicting data on the buffering and ergogenic properties of calcium lactate (CL), we investigated the effect of chronic CL supplementation on blood pH, bicarbonate, and high-intensity intermittent exercise performance. Sodium bicarbonate (SB) was used as a positive control. Eighteen athletes participated in this double-blind, placebo-controlled, crossover, fully counterbalanced study. All participants underwent three different treatments: placebo (PL), CL, and SB. The dose was identical in all conditions: 500 mg/kg BM divided into four daily individual doses of 125 mg/kg BM, for five consecutive days, followed by a 2-7-day washout period. On the fifth day of supplementation, individuals undertook four 30-s Wingate bouts for upper body with 3-min recovery between bouts. Total mechanical work (TMW) for the overall protocol and for the initial (1st+2nd) and final (3rd+4th) bouts was determined at each session. Blood pH, bicarbonate, and lactate levels were determined at rest, immediately and 5 min after exercise. CL supplementation did not affect performance (P > 0.05 for the overall TMW as well for initial and final bouts), nor did it affect blood bicarbonate and pH prior to exercise. SB supplementation improved performance by 2.9% for overall TMW (P = 0.02) and 5.9% in the 3rd+4th bouts (P = 0001). Compared to the control session, SB also promoted higher increases in blood bicarbonate than CL and PL (+0.03 ± 0.04 vs +0.009 ± 0.02 and +0.01 ± 0.03, respectively). CL supplementation was not capable of enhancing high-intensity intermittent performance or changing extracellular buffering capacity challenging the notion that this dietary supplement is an effective buffering agent.

Assuntos