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Contrary to its well-established role in declarative learning, the impact of sleep on motor memory consolidation remains a subject of debate. Current literature suggests that while motor skill learning benefits from sleep, consolidation of sensorimotor adaptation (SMA) depends solely on the passage of time. This has led to the proposal that SMA may be an exception to other types of memories. Here, we addressed this ongoing controversy in humans through three comprehensive experiments using the visuomotor adaptation paradigm (N = 290, 150 females). In Experiment 1, we investigated the impact of sleep on memory retention when the temporal gap between training and sleep was not controlled. In line with the previous literature, we found that memory consolidates with the passage of time. In Experiment 2, we used an anterograde interference protocol to determine the time window during which SMA memory is most fragile and, thus, potentially most sensitive to sleep intervention. Our results show that memory is most vulnerable during the initial hour post-training. Building on this insight, in Experiment 3, we investigated the impact of sleep when it coincided with the critical first hour of memory consolidation. This manipulation unveiled a benefit of sleep (30% memory enhancement) alongside an increase in spindle density and spindle-SO coupling during NREM sleep, two well-established neural markers of sleep consolidation. Our findings reconcile seemingly conflicting perspectives on the active role of sleep in motor learning and point to common mechanisms at the basis of memory formation.
Assuntos
Adaptação Fisiológica , Consolidação da Memória , Desempenho Psicomotor , Sono , Humanos , Feminino , Masculino , Consolidação da Memória/fisiologia , Sono/fisiologia , Adaptação Fisiológica/fisiologia , Adulto Jovem , Adulto , Desempenho Psicomotor/fisiologia , Destreza Motora/fisiologia , Aprendizagem/fisiologia , AdolescenteRESUMO
Apresentar, por meio de resenha analítica, a obra 'Consolidação, Simplificação e Revisão do Arco Normativo Infralegal da Saúde: um projeto, muitas vozes', publicado pelo CONASS e que narra a história biográfica de um projeto pioneiro e inovador da consolidação do arco normativo infralegal do Sistema Único de Saúde.
To present, through an analytical review, the work 'Consolidation, Simplification, and Review of the Infralegal Normative Arch of Health: one project, many voices', published by CONASS, which narrates the biographical history of a pioneering and innovative project in consolidating the infralegal normative arch of the Unified Health System.
Presentar, a través de una reseña analítica, la obra 'Consolidación, Simplificación y Revisión del Arco Normativo Infralegal de la Salud: un proyecto, muchas voces', publicada por CONASS y que narra la historia biográfica de un proyecto pionero e innovador en la consolidación del arco normativo infralegal del Sistema Único de Salud.
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Direito SanitárioRESUMO
BACKGROUND/OBJECTIVES: Tibial diaphysis fractures are common injuries resulting from high-to-low-energy traumas in patients of all age groups, but few reports currently provide complementary parameters for the assessment of bone healing processes in the postoperative period. Serum alkaline phosphatase (ALP) and the scores from the Radiographic Union Scale for Tibial Fractures (RUST) can promote new horizons in this context. Therefore, the aim of this study was to assess the behavior of ALP and RUST through within-subject comparisons from immediately post-surgery to 49 days after tibial diaphysis fracture repair. METHODS: This article included four case studies where patients underwent the same procedures. Adults of both sexes aged 18 to 60 years with tibial fractures requiring surgery were included. After surgical intervention (T1), the patients were followed for 49 days after surgery, returning for follow-up appointments on the 21st (T2) and 49th (T3) days. At the follow-up appointments, new X-ray images were obtained, and blood samples were collected for ALP measurement. RESULTS: Serum ALP levels increased by T2 following tibial reamed intramedullary nailing surgery. While this increase persisted into T3 for two patients, a decline was observed during the same period for the other two patients. Both events are indicative of the bone consolidation process, and RUST scores at the T3 corroborate this perspective for all patients included in this study. Considering that delta ALP (T3-T1 value) was lower in patients who exhibited the highest RUST score, we suggest that a synchronized analysis between ALP and RUST allows medics to diagnose bone consolidation. CONCLUSIONS: Therefore, it can be concluded that the analysis of ALP alongside RUST may be complementary for evaluating bone consolidation following tibial reamed intramedullary nailing surgery, but future studies are needed to confirm this assertion.
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AIM: to assess the small-scale 3D printing feasibility and cost estimation of a device for controlled dynamization. MATERIALS AND METHOD: The two-part device previously developed by our research group was printed with a carbon fiber-reinforced nylon filament (Gen3 CarbonX™ PA6+CF, 3DXTECH Additive Manufacturing) by a professional 3D printer (FUNMAT HT, Intamsys). Electricity, material, and labor costs for production in a Brazilian city in the Santa Catarina state were calculated. RESULTS: The devices for controlled dynamization were successfully printed in accordance with the planned design and dimensions. Six out of 38 printed devices presented defects in the bolt hole and were discarded. The average printing time per device was 1.9 h. The average electricity, material, and labor costs per printed device were respectively US$0.71, US$13.55, and US$3.04. The total production cost per device reaches approximately US$20 by adding the average cost of defective devices (15 %). CONCLUSION: 3D printing of the controlled dynamization device is feasible and its cost seems affordable to most healthcare services, which could optimize the consolidation of diaphyseal fractures and reduce treatment time for patients.
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Estudos de Viabilidade , Impressão Tridimensional , Impressão Tridimensional/economia , Humanos , Desenho de Equipamento , Fixadores Externos/economia , Fixação de Fratura/instrumentação , Fixação de Fratura/métodos , Fixação de Fratura/economia , Brasil , Fraturas Ósseas/cirurgiaRESUMO
BACKGROUND: Acute myeloid leukaemia (AML) is considered a costly disease. Depending on the risk stratification, the patient may receive consolidation with cycles of intermediate doses of cytarabine, auto-HSCT or allo-HSCT according to availability in each service and the availability of a compatible donor. Literature data indicate that safety and effectiveness do not differ between consolidation therapy with intermediate-dose cytarabine or auto-HSCT, and so the cost can help physicians and health managers in their choice. METHOD: The cost of the second consolidation was compared in 18 to 60-year-old patients with de novo AML who were included in the International Consortium of Acute Myeloid Leukaemia (ICAML) protocol. Patients treated with auto-HSCT or intermediate doses of cytarabine (IDAC) were analysed during four years using the microcosting methodology. RESULTS: The mean costs for auto-HSCT and IDAC were BRL$ 34,900.95 (range: 23,611.36-41,229.59) and 15,231.64 (range: 6,546.36-23,253.53), respectively. The mean duration of in-hospital stay was 88.4 (93-133) and 94 (50-153) days, respectively. The mean cost of the four cycles of treatment was BRL$ 114.212,78 for auto-HSCT and BRL$ 121.980,93 for the chemotherapy group. Regardless of the type of treatment, the input that had the greatest economic impact was hospital admission, mainly due to infections. CONCLUSION: Auto-HSCT had a lower average cost per patient and hospitalization rate than chemotherapy.
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Growing evidence indicates a critical role of astrocytes in learning and memory. However, little is known about the role of basolateral amygdala complex (BLA-C) astrocytes in contextual fear conditioning (CFC), a paradigm relevant to understand and generate treatments for fear- and anxiety-related disorders. To get insights on the involvement of BLA-C astrocytes in fear memory, fluorocitrate (FLC), a reversible astroglial metabolic inhibitor, was applied at critical moments of the memory processing in order to target the acquisition, consolidation, retrieval and reconsolidation process of the fear memory. Adult Wistar male rats were bilaterally cannulated in BLA-C. Ten days later they were infused with different doses of FLC (0.5 or 1 nmol/0.5 µl) or saline before or after CFC and before or after retrieval. FLC impaired fear memory expression when administered before and shortly after CFC, but not one hour later. Infusion of FLC prior and after retrieval did not affect the memory. Our findings suggest that BLA-C astrocytes are critically involved in the acquisition/early consolidation of fear memory but not in the retrieval and reconsolidation. Furthermore, the extinction process was presumably not affected (considering that peri-retrieval administration could also affect this process).
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Astrócitos , Complexo Nuclear Basolateral da Amígdala , Medo , Memória , Ratos Wistar , Animais , Medo/fisiologia , Medo/efeitos dos fármacos , Astrócitos/efeitos dos fármacos , Astrócitos/fisiologia , Masculino , Complexo Nuclear Basolateral da Amígdala/efeitos dos fármacos , Complexo Nuclear Basolateral da Amígdala/fisiologia , Ratos , Memória/fisiologia , Memória/efeitos dos fármacos , Citratos/farmacologia , Condicionamento Clássico/efeitos dos fármacos , Condicionamento Clássico/fisiologia , Consolidação da Memória/fisiologia , Consolidação da Memória/efeitos dos fármacos , Tonsila do Cerebelo/efeitos dos fármacos , Tonsila do Cerebelo/fisiologia , Extinção Psicológica/efeitos dos fármacos , Extinção Psicológica/fisiologiaRESUMO
Alzheimer's disease is the most common neurodegenerative disease, and its treatment is lacking. In this work, we tested Amylovis-201, a naphthalene-derived compound, as a possible therapeutic candidate for the treatment of AD. For this purpose, we performed three experiments. In the first and third experiment, animals received a bilateral administration of streptozotocin and, starting 24 h after injection, a daily dose of Amylovis-201 (orally), for 17 days or for the whole time of the experiment respectively (28 days), after which learning and memory, as well as the number of hippocampal dentate gyrus cells, were assessed. In the second experiment, healthy animals received a single dose of Amylovis-201, 10 min or 5 h after the learning section to assess whether this substance could promote specific mechanisms involved in memory trace formation. Our data show that, administration of a single dose of Amylovis-201, 10 min after the end of training, but not at 5 h, produces a prolongation in memory duration, probably because it modulates specific mechanisms involved in memory trace consolidation. Furthermore, daily administration of Amylovis-201 to animals with bilateral intracerebroventricular injection of STZ produces a reduction in the loss of the hippocampus dentate gyrus cells and an improvement in spatial memory, probably because Amylovis-201 can interact with some of the protein kinases of the insulin signaling cascade, also involved in neural plasticity, and thereby halt or reverse some of the effects of STZ. Taking to account these results, Amylovis-201 is a good candidate for the therapeutic treatment of AD.
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Doença de Alzheimer , Doenças Neurodegenerativas , Animais , Estreptozocina/farmacologia , Doenças Neurodegenerativas/metabolismo , Modelos Animais de Doenças , Hipocampo/metabolismo , Memória Espacial , Transtornos da Memória/metabolismo , Aprendizagem em LabirintoRESUMO
Corticosterone (CORT) release during learning experiences is associated with strong memories and activity of the glucocorticoid receptor. It has been shown that lesions of the dorsal striatum (DS) of rats trained in the cued version of the Morris water maze impair memory, and that local injection of CORT improves its performance, suggesting that DS activity is involved in procedural memory which may be modulated by CORT. We trained rats in cued Morris water maze and analyzed the effect of CORT synthesis inhibition on performance, CORT levels, expression of plasticity-involved genes, such as the brain derived neurotrophic factor (BDNF), casein kinase 2 (CK2), and the serum/glucocorticoid regulated kinase 1 (SGK1), as well as the presence of phosphorylated nuclear glucocorticoid receptor in serine 232 (pGR-S232) in the DS. The inhibition of CORT synthesis by metyrapone reduced CORT levels in plasma, prevented its increment in DS and impaired the performance of cued water maze. Additionally, there was an increase of CK2 and SGK1 mRNAs expression in trained subjects, which was unrelated to CORT levels. Finally, we did not observe changes in nuclear pGR-S232 in any condition. Our findings agree with evidence demonstrating that decreasing CORT levels hinders acquisition and consolidation of the spatial version of the Morris water maze; these novel findings broaden our knowledge about the involvement of the DS in the mechanisms underlying procedural memory.
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Memory and learning allow animals to appropriate certain properties of nature with which they can navigate in it successfully. Memory is acquired slowly and consists of two major phases, a fragile early phase (short-term memory, <4 h) and a more robust and long-lasting late one (long-term memory, >4 h). Erythropoietin (EPO) prolongs memory from 24 to 72 h when animals are trained for 5 min in a place recognition task but not when training lasted 3 min (short-term memory). It is not known whether it promotes the formation of remote memory (≥21 days). We address whether the systemic administration of EPO can convert a short-term memory into a long-term remote memory, and the neural plasticity mechanisms involved. We evaluated the effect of training duration (3 or 5 min) on the expression of endogenous EPO and its receptor to shed light on the role of EPO in coordinating mechanisms of neural plasticity using a single-trial spatial learning test. We administered EPO 10 min post-training and evaluated memory after 24 h, 96 h, 15 days, or 21 days. We also determined the effect of EPO administered 10 min after training on the expression of arc and bdnf during retrieval at 24 h and 21 days. Data show that learning induces EPO/EPOr expression increase linked to memory extent, exogenous EPO prolongs memory up to 21 days; and prefrontal cortex bdnf expression at 24 h and in the hippocampus at 21 days, whereas arc expression increases at 21 days in the hippocampus and prefrontal cortex.
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Eritropoetina , Consolidação da Memória , Animais , Fator Neurotrófico Derivado do Encéfalo/metabolismo , Eritropoetina/farmacologia , Eritropoetina/metabolismo , Receptores da Eritropoetina/metabolismo , Encéfalo/metabolismo , Hipocampo/metabolismo , Memória de Longo PrazoRESUMO
It has been shown that land fragmentation can negatively impact the efficiency of farming. Therefore, experts recommend land consolidation process, as a logical and workable solution to solve the problems and complications caused by land fragmentation. Land levelling and consolidation is a process of land reform that changes the construction of agricultural lands which leads to rural development through reforming farm management. However, a single plan cannot be applied to different regions, even though they might be in the same country. Hence, it is vital to investigate multiple factors in a certain region to devise the perfect consolidation plan. The present study, which is a survey-exploratory research, is conducted to provide a comprehensive model to implement the plan for levelling and consolidation of agricultural lands in the Abu Fazel region of Ahvaz, Iran. This research is an applied field research which uses both library and field methods to collect the required data. The study population is in Abu Fazel in the northeast of Ahvaz in Zargan region. The results of the study show that cultural, social, economic, policy-making, educational, agricultural and managerial factors have an effect on the participation of farmers in the levelling and consolidation of agricultural lands in the study area (p≥0.01). Also, there is a strong positive relationship between these factors and the farmers' participation in levelling and consolidation of agricultural lands (p≥0.01). Among these factors, it is observed that policy is main factor. Policymakers can play an effective role in land consolidation and macro development on the one hand and agricultural and rural development. On the other, by accurately assessing the interactive effect of land consolidation and related factors, along with the effects of this process on the evolution of agronomic systems.
Tem sido demonstrado que a fragmentação da terra pode impactar negativamente a eficiência da agricultura. Portanto, os especialistas recomendam o processo de consolidação de terras como uma solução lógica e viável para resolver os problemas e as complicações causadas pela fragmentação de terras. O nivelamento e a consolidação da terra são processos de reforma agrária que alteram a construção de terras agrícolas, o que leva ao desenvolvimento rural por meio da reforma da gestão agrícola. No entanto, um único plano não pode ser aplicado a diferentes regiões, mesmo que estejam no mesmo país. Portanto, é vital investigar vários fatores em determinada região para elaborar o plano de consolidação perfeito. O presente estudo, que é uma pesquisa exploratória de levantamento, é realizado para fornecer um modelo abrangente para implementar o plano de nivelamento e consolidação de terras agrícolas na região de Abu Fazel, de Ahvaz, no Irã. Trata-se de uma pesquisa de campo aplicada que usa métodos de biblioteca e de campo para coletar os dados necessários. A população do estudo está em Abu Fazel, no nordeste de Ahvaz, na região de Zargan. Os resultados do estudo mostram que fatores culturais, sociais, econômicos, políticos, educacionais, agrícolas e gerenciais influenciam a participação dos agricultores no nivelamento e na consolidação das terras agrícolas na área de estudo (p ≥ 0,01). Além disso, há forte relação positiva entre esses fatores e a participação dos agricultores no nivelamento e na consolidação das terras agrícolas (p ≥ 0,01). Entre esses fatores, observa-se que a política é o principal deles. Os formuladores de políticas, por um lado, podem desempenhar um papel efetivo na consolidação da terra e no macrodesenvolvimento e desenvolvimento agrícola e rural e, por outro, avaliar com precisão o efeito interativo da consolidação de terras e fatores relacionados, juntamente com os efeitos desse processo na evolução dos sistemas agronômicos.