RESUMO
Antimicrobial resistance and biofilm formation by microbial pathogens pose a significant challenge to poultry production systems due to the persistent risk of dissemination and compromise of bird health and productivity. In this context, the study aimed to investigate the occurrence of different multiresistance phenotypes and the biofilm-forming ability of Enterobacteriaceae isolated from broiler chicken excreta in poultry production units in Ceará, Brazil. Samples were collected from three distinct broiler breeding facilities and subjected to isolation, identification, antibiotic susceptibility testing, phenotypic screening for ß-lactamases enzymes, and biofilm formation evaluation. Seventy-one strains were identified, being Escherichia coli (37 %) and Proteus mirabilis (32 %), followed by Klebsiella pneumoniae (11 %), Providencia stuartii (9 %), Klebsiella aerogenes (6 %), Alcaligenes faecalis (4 %), and Salmonella sp. (1 %). A significant proportion (87 %) of multiresistant strains were detected. For the phenotypic evaluation of ß-lactamases production, strains with resistance to second and third-generation cephalosporins and carbapenems were tested. About 4 of 6 and 10 of 26 were positive for inducible chromosomal AmpC ß-lactamase and extended-spectrum ß-lactamase (ESBL), respectively. Regarding biofilm formation, it was observed that all MDR strains were capable of forming biofilm. In this sense the potential of these MDR bacteria to develop biofilms becomes a significant concern, representing a real threat to both human and animal health, as biofilms offer stability, antimicrobial protection, and facilitate genetic transfer.
Assuntos
Antibacterianos , Biofilmes , Galinhas , Farmacorresistência Bacteriana Múltipla , Enterobacteriaceae , Fazendas , Fezes , Testes de Sensibilidade Microbiana , beta-Lactamases , Animais , Biofilmes/crescimento & desenvolvimento , Biofilmes/efeitos dos fármacos , Brasil , beta-Lactamases/genética , beta-Lactamases/metabolismo , Fezes/microbiologia , Enterobacteriaceae/efeitos dos fármacos , Enterobacteriaceae/isolamento & purificação , Enterobacteriaceae/genética , Antibacterianos/farmacologia , Galinhas/microbiologia , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Aves Domésticas/microbiologia , Infecções por Enterobacteriaceae/microbiologia , Infecções por Enterobacteriaceae/veterináriaRESUMO
RESEARCH HIGHLIGHTS: Peptides + CpG-ODN reduced SH in caeca at the first week post-infection.Administered formulations did not reduce SH-faecal excretion.Levels of intestinal IgA were similar between all groups.CpG-ODN improved some parameters associated with chick intestinal health.
Assuntos
Doenças das Aves Domésticas , Salmonelose Animal , Salmonella enterica , Animais , Sorogrupo , Salmonelose Animal/prevenção & controle , Salmonelose Animal/microbiologia , Doenças das Aves Domésticas/prevenção & controle , Doenças das Aves Domésticas/microbiologia , GalinhasRESUMO
Increased meat and egg production leads to concomitant changes in poultry practices, including the indiscriminate use of formaldehyde to sanitize hatching eggs. Although this sanitizer aids in the increase in poultry production, its toxic potential for man and for avian embryos represents an obstacle to its long-term use. This review assesses whether essential oils fit into the context of hatching egg contamination, reviewing their antimicrobial efficiency, toxicity to poultry embryos and chicks, and their sanitizing effects on poultry production parameters. Studies have indicated that, because they are safer, most of the essential oils studied can be a potential substitute for formaldehyde for minimizing microbial exposure of hatching eggs and embryos. However, complementary studies on the microbiological profile of embryos and chicks hatched from eggs sanitized with essential oils need to be carried out and the economic feasibility of the candidate products should also be considered.
RESUMO
Recent advances in poultry practice have produced new tools enabling the poultry industry to increase productivity. Aiming at increasing production quality, varying protocols of in ovo injection facilitate the introduction of exogenous substances into the egg to complement the nutrients that support embryonic development up to hatching, which are already available in the internal and external compartments. Due to embryonic sensitivity, adding any substance into the egg can be either advantageous or disadvantageous for embryonic survival and can influence hatch rates. Thus, understanding the relationship between poultry practices and production rates is the first step towards successful commercial application. This review aims to assess the influence on hatch rates of injecting different substances in ovo, including effects on embryo and chick health parameters where these are reported. Bibliographic mappings of co-authorship of citations, co-occurrence of keywords, and bibliographic coupling based on the in ovo injection technique and hatchability parameters were also performed. Using the Scopus database, 242 papers were retrieved, reviewed, and submitted for bibliographic mapping using the VOSviewer® software. This review provides a broad overview of just over 38 years' research on the subject, revealing that studies have significantly increased and peaked in 2020, being produced primarily by US researchers and published primarily in the journal Poultry Science. It also reveals that despite negative reports relating to some substances in the embryo, in ovo delivery of substances may possibly change the poultry industry for the better in terms of production rates (hatchability) and/or poultry health.
RESUMO
Salmonella is the main human pathogen present in the poultry chain. Salmonella Heidelberg is one of the most important serovars for public health since it has been frequently isolated in broiler chickens from different countries and may present multidrug resistance (MDR). This study was carried out with 130 S. Heidelberg isolates collected from pre-slaughter broiler farms in 2019 and 2020 in 18 cities from three Brazilian states to study relevant aspects regarding their genotypic and phenotypic resistance. The isolates were tested and identified using somatic and flagellar antiserum (0:4, H:2, and H:r), and an antimicrobial susceptibility test (AST) was performed against 11 antibiotics for veterinary use. The strains were typed by Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR, and representatives of the main clusters of the identified profiles were sequenced by Whole Genome Sequencing (WGS). AST results showed that all isolates were resistant to sulfonamide, 54 % (70/130) were resistant to amoxicillin, and only one was sensitive to tetracycline. Twelve isolates (15.4 %) were MDR. The dendrogram obtained from the ERIC-PCR showed that the strains were grouped into 27 clusters with similarity above 90 %, with some isolates showing 100 % similarity but with different phenotypic profiles of antimicrobial resistance. Identical strains collected on the same farm on other dates were identified, indicating that they were residents. WGS identified 66 antibiotic-resistance genes. The sul2 (present in all sequenced samples) and tet(A) genes were highlighted and validated in the experimental analysis. The fosA7 gene was also identified in all sequenced samples, but resistance was not observed in the phenotypic test, possibly due to the heteroresistance of the S. Heidelberg strains evaluated. Considering that chicken meat is one of the most consumed meats in the world, the data obtained in the present study can corroborate the mapping of the origin and trends of antimicrobial resistance.
Assuntos
Galinhas , Farmacorresistência Bacteriana Múltipla , Animais , Humanos , Brasil , Farmacorresistência Bacteriana Múltipla/genética , Galinhas/microbiologia , Testes de Sensibilidade Microbiana , Salmonella , Antibacterianos/farmacologiaRESUMO
The aim of this study was to predict production indicators and to determine their potential economic impact on a poultry integration system using artificial neural networks (ANN) models. Forty zootechnical and production parameters from broiler breeder farms, one hatchery, broiler production flocks, and one slaughterhouse were selected as variables. The ANN models were established for four output variables: "saleable hatching", "weight at the end of week 5," "partial condemnation," and "total condemnation" and were analyzed in relation to the coefficient of multiple determination (R2), correlation coefficient (R), mean error (E), mean squared error (MSE), and root mean square error (RMSE). The production scenarios were simulated and the economic impacts were estimated. The ANN models were suitable for simulating production scenarios after validation. For "saleable hatching", incubator and egg storage period are likely to increase the financial gains. For "weight at the end of the week 5" the lineage (A) is important to increase revenues. However, broiler weight at the end of the first week may not have a significant influence. Flock sex (female) may influence the "partial condemnation" rates, while chick weight at first day may not. For "total condemnation", flock sex and type of chick may not influence condemnation rates, but mortality rates and broiler weight may have a significant impact.
O objetivo deste trabalho foi predizer os indicadores de produção e determinar o seu potencial impacto econômico em um sistema de integração utilizando as redes neurais artificiais (RNA). Quarenta parâmetros zootécnicos e de produção de granjas de matrizes e de frango de corte, um incubatório e um abatedouro foram selecionados como variáveis. Os modelos de RNA foram estabelecidos para quatro variáveis de saída ("eclosão vendável", "peso ao final da quinta semana", "condenações parciais" e "condenações totais") e foram analisados em relação ao coeficiente de determinação múltipla (R2), coeficiente de correlação (R), erro médio (E), erro quadrático médio (EQM) e raiz do erro quadrático médio (REQM). Os cenários produtivos foram simulados e os impactos foram estimados. Os modelos de RNA gerados foram adequados para simular diferentes cenários produtivos após o treinamento. Para "eclosão vendável", o modelo de incubadora e o período de incubação aumentaram os ganhos financeiros. Para "peso ao final da quinta semana", a linhagem também demonstrou influencia no retorno financeiro, o que não aconteceu com o peso ao final da primeira semana. O sexo do lote possui influência nas taxas de "condenação parcial", ao contrário do peso do frango no primeiro dia. As taxas de mortalidade e o peso do frango apresentaram influência na "condenação total", mas o sexo do lote e o tipo de pinto não tiverem influência.
Assuntos
Animais , Aves Domésticas , Inteligência Artificial , Redes Neurais de ComputaçãoRESUMO
The sanitization of hatching eggs is the backbone of the hygienic-sanitary management of eggs on farms and extends to the hatchery. Poultry production gains depend on the benefits of sanitizers. Obtaining the maximum yield from incubation free of toxic sanitizers is a trend in poultry farming, closely following the concerns imposed through scientific research. The toxic characteristics of formaldehyde, the primary sanitizer for hatching eggs, are disappointing, but it is a cheap, practical and widely used antimicrobial. To overcome this shortcoming, multiple synthetic and natural chemical sanitizers have been, and continue to be, tested on hatching eggs. This review aims to evaluate the effects of different sanitizers on the microbiological quality of hatching eggshells and poultry health during embryogenesis and early stages after hatching.
RESUMO
Despite the lack of large-scale farming of free-range chickens in Brazil, their production generates income in the countryside and prevents exodus of rural families in agricultural regions. The objective of this study is to evaluate the economic viability of free-range broiler production in different facilities. The experiment was conducted in two different sheds (masonry shed-SM and wooden shed-SW) located in the Plural Space of the Universidade Federal do Vale São Francisco, municipality of Juazeiro, BA. Here, 200 heavy red French free-range chickens were distributed in the two sheds and were raised from the 1st to the 88th day (slaughter). Assuming that the minimum age for slaughter is 85 days, the results indicated that at least 205 birds in SM and 217 birds in SW were necessary for the producer to earn the minimum per capita monthly wage in Brazil (2020); at least 411 birds in CG and 600 birds in GM were found to be necessary to achieve maximum productivity at the end of the production cycle. The maximum profitability in the slaughter of the chickens was achieved at an age of 60 days.
RESUMO
There is increasing societal concern regarding the negative impact of intensive poultry production on animal welfare, human health, and on the environment. This is leading to the inclusion of animal welfare as an imperative aspect for sustainable production. Certain environmental factors may challenge domesticated birds, resulting in poor health and welfare status. Resilience is the capacity to rapidly return to prechallenge status after coping with environmental stressors, thus resilient individuals have better chances to maintain good health and welfare. Immune-neuroendocrine system, thoroughly characterized in the domestic bird species, is the physiological scaffold for stress coping and health maintenance, influencing resilience and linking animal welfare status to these vital responses. Modern domestic bird lines have undergone specific genetic selective pressures for fast-growing, or high egg-production, leading to a diversity of birds that differ in their coping capacities and resilience. Deepening the knowledge on pro/anti-inflammatory milieus, humoral/cell-mediated immune responses, hormonal regulations, intestinal microbial communities and mediators that define particular immune and neuroendocrine configurations will shed light on coping strategies at the individual and population level. The understanding of the profiles leading to differential coping and resilience potential will be highly relevant for improving bird health and welfare in a wider range of challenging scenarios and, therefore, crucial to scientifically tackle long term sustainability.
Assuntos
Galinhas , Aves Domésticas , Adaptação Psicológica , Bem-Estar do Animal , Animais , Humanos , Sistemas Neurossecretores , Aves Domésticas/fisiologiaRESUMO
The study of non-typhoid Salmonella in broiler integrations has been limited by the resolution of typing techniques. Although serotyping of Salmonella isolates is used as a traditional approach, it is not of enough resolution to clearly understand the dynamics of this pathogen within poultry companies. The aim of this research was to investigate the epidemiology and population dynamics of Salmonella serotypes in 2 poultry integrations using a whole genome sequencing approach. Two hundred and forty-three Salmonella isolates recovered from the broiler production chain of 2 integrated poultry companies were whole genome sequenced and analyzed with dedicated databases and bioinformatic software. The analyses of sequences revealed that S. Infantis was the most frequent serotype (82.3%). Most isolates showed a potential for resistance against medically important antibiotics and disinfectants. Furthermore, 97.5% of isolates harbored the pESI-like mega plasmid, that plays an important role in the global dissemination of AMR. SNP tree analysis showed that there were clones that are niche-specific while other ones were distributed throughout the broiler production chains. In this study, we demonstrated the potential of whole genome sequencing analysis for a comprehensive understanding of Salmonella distribution in integrated poultry companies. Data obtained with these techniques allow determination of the presence of genetic factors that play an important role in the environmental fitness and pathogenicity of Salmonella.