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Objetivo: analisar as representações sociais da autoproteção profissional e pessoal dos enfermeiros no contexto da Covid-19. Método: estudo descritivo, com abordagem qualitativa, pautado na Teoria das Representações Sociais, abordagem processual. Participaram 30 enfermeiros que realizaram cuidados de enfermagem a pacientes com Covid-19, no Estado do Rio de Janeiro. A coleta de dados ocorreu por meio de entrevista semiestruturada entre abril e maio 2021, realizada em plataforma online. O tratamento dos dados foi realizado com a técnica de análise de conteúdo temático-categorial. Resultado: a análise resultou em quatro categorias: Representações sociais e práticas relativas a Covid-19; Representações sociais da autoproteção; Desafios no enfrentamento da Covid-19: autoproteção pessoal e profissional; e Estrutura e fluxo de atendimento e sua relação com a autoproteção profissional. Considerações finais: houve engajamento dos profissionais no uso dos EPI na pandemia, o que aponta uma preocupação quanto à autoproteção na prestação de cuidados à pacientes com a Covid-19.
Objective: to analyze the social representations of professional and personal self-protection among nurses in the context of Covid-19. Method: a descriptive study with a qualitative approach, based on the Social Representations Theory, using a procedural approach. Thirty nurses who provided nursing care to Covid-19 patients in the State of Rio de Janeiro participated in the study. Data collection took place through semi-structured interviews between April and May 2021, conducted on an online platform. Data analysis was performed using the thematic-categorical content analysis technique. Results: The analysis resulted in four categories of social representations and practices related to challenges in coping with Covid-19: personal and professional self-protection; Care structure and flow and its relationship with professional self-protection. Final considerations: There was engagement of professionals in the use of PPE during the pandemic, indicating a concern regarding self-protection in providing care to patients with Covid-19.
Objetivo: analizar las representaciones sociales de la autoprotección profesional y personal de los enfermeros en el contexto del Covid-19. Método: estudio descriptivo, con enfoque cualitativo, basado en la Teoría de las Representaciones Sociales, enfoque procesal. Participaron 30 enfermeros que brindaron cuidados de enfermería a pacientes con Covid-19 en el Estado de Río de Janeiro. La recolección de datos se llevó a cabo a través de entrevistas semiestructuradas entre abril y mayo de 2021, realizadas en una plataforma online. El procesamiento de los datos se realizó mediante la técnica de análisis de contenido temático categorial. Resultado: del análisis surgieron cuatro categorías: Representaciones sociales y prácticas relacionadas con el Covid-19; Representaciones sociales de la autoprotección; Desafíos de enfrentar el Covid-19: autoprotección personal y profesional; y Estructura y flujo de atención y su relación con la autoprotección profesional. Consideraciones finales: los profesionales demostraron compromiso en el uso de EPP durante la pandemia, lo que indica que se preocupaban por autoprotegerse durante la atención a pacientes con Covid-19.
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This study investigates the efficacy of nebivolol (NBV) in experimental models of toxoplasmosis, focusing on parasite burden reduction and neuronal protection. In the acute model of experimental toxoplasmosis, Swiss mice infected with RH strain tachyzoites received oral NBV chlorhydrate doses of 2 mg/kg/day and 4 mg/kg/day for 8 days. Treatment with NBV significantly reduced parasite burden compared to vehicle and standard drug (PYR) groups. In the chronic model of experimental toxoplasmosis, C57/BL6 mice infected with the ME49 strain received NBV chlorhydrate 41 days post-infection and were evaluated after 10 days of treatment. NBV chlorhydrate effectively reduced cyst number and area, as well as bradyzoite burden compared to controls. Histological analysis demonstrated that NBV chlorhydrate preserved neuronal count, with the 4 mg/kg/day dose yielding counts similar to non-infected mice. Statistical analysis confirmed significant differences compared to control groups. Furthermore, immunohistochemical analysis revealed a significant reduction in iNOS labeling in the brains of mice treated with NBV chlorhydrate, indicating a decrease in nitric oxide production compared to control groups. These findings suggest NBV's potential as a promising candidate for toxoplasmosis treatment, highlighting its ability to reduce parasite burden and protect neuronal integrity. Further research is warranted to elucidate NBV's mechanisms of action and its clinical application in managing toxoplasmosis.
Assuntos
Encéfalo , Modelos Animais de Doenças , Camundongos Endogâmicos C57BL , Nebivolol , Carga Parasitária , Toxoplasmose Animal , Animais , Nebivolol/farmacologia , Nebivolol/uso terapêutico , Camundongos , Toxoplasmose Animal/tratamento farmacológico , Toxoplasmose Animal/parasitologia , Encéfalo/parasitologia , Encéfalo/patologia , Encéfalo/efeitos dos fármacos , Feminino , Neurônios/efeitos dos fármacos , Neurônios/parasitologia , Etanolaminas/farmacologia , Etanolaminas/uso terapêutico , Antiprotozoários/farmacologia , Antiprotozoários/uso terapêutico , Antiprotozoários/administração & dosagem , Benzopiranos/farmacologia , Benzopiranos/uso terapêutico , Resultado do Tratamento , Óxido Nítrico/metabolismo , Toxoplasma/efeitos dos fármacos , Óxido Nítrico Sintase Tipo II/metabolismoRESUMO
OBJECTIVE: To identify, map, and describe studies involving Brazilian children in early childhood in situations of social vulnerability. SOURCE OF DATA: A scoping review including full articles published in Portuguese and English up to March 2023, with no temporal restrictions. Searches were conducted in the MEDLINE/PubMed, Scielo, EMBASE, Cochrane, Scopus, CINAHL, Web of Science, PEDro, and LILACS databases. Journal metrics, sample characteristics, study area, characterization of the situation of social vulnerability, and study outcomes were extracted. SUMMARY OF THE FINDINGS: Seventy-six articles involving a total of 107.740 children in early childhood were included in this study. These studies presented relevant findings, including the temporal publication trend, the variability of social vulnerability indicators, the scarcity of intervention studies, and the fact that 100% of eligible studies were from the health area. Negative outcomes were associated with the condition of social vulnerability in almost the entire sample, reinforcing the need for government policies capable of protecting early childhood from the effects of social vulnerability. CONCLUSION: This scoping review mapped important findings involving Brazilian children in social vulnerability. It also identified literature gaps such as the need for intervention and multisectoral studies among health, education, and social protection.
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Chalcone (E)-1,3-diphenyl-prop-2-en-1-one and a series of 14 methoxylated derivatives have been synthesized via Claisen-Schmidt aldol condensation and characterized by FTIR, CG/MS/DIC, 1D (1H and 13C), 2D (COSY, HSQC, and HMBC) NMR, and EMAR techniques. All molecules were tested at 1mM concentration for antifungal (Sclerotium sp., Macrophomina phaesolina and Colletotrichum gloeosporioides), antibacterial (Acidovorax citrulli two strains), and antiprotozoal (Phytomonas serpens) activities. Unmodified chalcone (CH0) and derivatives CH1, CH2, CH8 stood out in terms of antifungal activity. CH0 presented IC50 values of 47.3 µM (9.8 µg/mL) for the fungus C. gloeosporioides. In addition, fluorescence microscopy indicated that CH0 promoted loss of hyphal cell membrane integrity. The CH1 and CH2 derivatives promoted the inhibition of Sclerotium sp. with IC50 of 127.5 µM (32.9 µg/mL) and 110.4 µM (29.6 µg/mL), respectively. All molecules showed high activity against the phytoparasite P. serpens with IC50 values of 0.98, 2.40, 10.25, and 3.11 µM for the derivatives CH2, CH3, CH5 and CH14 respectively. The results demonstrated that derivatives methoxylated in both rings (CH2) as well as derivatives with a furan ring associated with the methoxy group in ring A, as well as unmodified chalcone can be promising agricultural fungicides for controlling the fungi studied.
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Efforts are intensifying to identify bioactive microbial metabolites from biocontrol agents to manage plant pathogens in critical crops. This study examined both volatile organic compounds (VOCs) and non-volatile compounds from Metarhizium carneum and Lecanicillium uredinophilum strains for their antimicrobial effects against various phytopathogens and analyzed their exo-metabolomes. M. carneum VOCs inhibited four bacterial and eight fungal species by up to 45.45%, while L. uredinophilum VOCs inhibited five bacterial and eight fungal species by up to 50.91%. Additionally, n-BuOH extracts from both biocontrol agents effectively targeted three fungi and five bacteria. The exo-metabolomes of M. carneum and L. uredinophilum included 125 and 102 spectrometric features, respectively, primarily consisting of polyketides, alkaloids, lipids, organic aromatic compounds, terpenoids, and peptides. Our findings revealed a correlation between the phylogenetic relationships of M. carneum strains, their bioactivity patterns against phytopathogens, and their metabolomic profiles. Notably, some compounds detected in both fungi previously demonstrated biological activity against plant pathogens, enhancing their biocontrol potential. This study not only evidences the antimicrobial properties of diffusible compounds from M. carneum and L. uredinophilum, but also documents the antimicrobial potential of their VOCs for the first time, supporting their use in sustainable agricultural practices, reducing reliance on chemical inputs.
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Objetivo: Determinar las conductas sexuales protectoras en estudiantes de una universidad de Chillán, Chile. Métodos: Estudio cuantitativo, descriptivo con un muestreo probabilístico por conveniencia de 234 estudiantes universitarios, para los criterios de inclusión destaca haber iniciado actividad sexual. La recolección de datos fue a través de Google forms. El instrumento utilizado fue "Cuestionario de conducta sexual segura". Resultados: Del total de los alumnos encuestados el 64,1 % son mujeres. En cuanto al estado civil, el 93,5 % se identifica soltero. Solo el 47,4 % detiene la actividad sexual para utilizar condón. El 80,8 % evita relaciones sexuales en la primera cita, un 81,6 % nunca tienen relaciones anales sin condón, y el 99,6 % nunca ha tenido sexo con personas que utilizan drogas. Se encontró que la media para conducta sexual protectora es de 70,2 %. Conclusión: Los encuestados presentan conductas sexuales protectoras(AU)
Objective: To determine protective sexual behaviors in students at a university in Chillán, Chile. Methods: Quantitative, descriptive study with a probability sampling by convenience of 234 university students, for the inclusion criteria it stands out to have initiated sexual activity. Data collection was through Google form. The instrument used was the "Safe sexual behavior questionnaire". Results: Of the total students surveyed, 64.1% are women. Regarding marital status, 93.5% identify themselves as single. Only 47.4% stop sexual activity to use a condom. 80.8% avoid sexual relations on the first date, 81.6% never have anal relations without a condom, and 99.6% have never had sex with people who use drugs. It was found that the mean for protective sexual behavior is 70.2 %. Conclusion: The respondents present protective sexual behaviors(AU)
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Humanos , Masculino , Feminino , Adolescente , Adulto , Comportamento Sexual , Estudantes , Universidades , Preservativos , Educação Sexual , Coito , ComunicaçãoRESUMO
La pandemia de COVID-19 representó un gran desafío para los profesionales de la salud de Perú, debido al riesgo elevado de contagio, la sobrecarga laboral y afectación de la salud mental. Objetivo: describir las experiencias laborales y percepciones del personal sanitario de este país durante la pandemia de COVID-19. Materiales y Métodos: se llevó a cabo un estudio cualitativo fenomenológico, en el que se realizaron ocho entrevistas semiestructuradas a profesionales de la salud pública en Perú entre mayo y diciembre del año 2020. Las mismas fueron grabadas y transcritas para la recolección de la información, las cuales se analizaron en categorías. Resultados: en las entrevistas realizadas se destacaron los desafíos organizativos y de recursos, como la escasez de equipos de protección personal y gestión burocrática. Se resaltó el impacto humano y social en términos de comunicación, empatía y reevaluación de valores personales. Se observó adaptaciones significativas en la práctica médica, con énfasis en el manejo de pacientes y la capacitación. Conclusiones: las experiencias clínicas y emocionales reflejaron los retos y motivaciones del personal sanitario durante la pandemia; en el que se demostró la necesidad de una mayor adaptabilidad y preparación, así como la importancia del bienestar emocional y social.
The COVID-19 pandemic represented a great challenge for health professionals in Peru, due to the high risk of contagion, work overload, and impact on mental health. Objective: describe the work experiences and perceptions of health personnel in this country during the COVID-19 pandemic. Materials and Methods: a qualitative phenomenological study was carried out, in which eight semi-structured interviews were carried out with public health professionals in Peru between May and December 2020. They were recorded and transcribed for the collection of information, which were analyzed in categories. Results: organizational and resource challenges were highlighted in the interviews conducted, such as the shortage of personal protective equipment and bureaucratic management. The human and social impact was highlighted in terms of communication, empathy and reevaluation of personal values. Significant adaptations were observed in medical practice, with emphasis on patient management and training. Conclusions: the clinical and emotional experiences reflected the challenges and motivations of healthcare personnel during the pandemic; in which the need for greater adaptability and preparation was demonstrated, as well as the importance of emotional and social well-being
A pandemia da COVID-19 representou um grande desafio para os profissionais de saúde no Peru, devido ao alto risco de contágio, sobrecarga de trabalho e impacto na saúde mental. Objetivo: descrever as experiências de trabalho e percepções do pessoal de saúde neste país durante a pandemia da COVID-19. Materiais e Métodos: Foi realizado um estudo fenomenológico qualitativo, no qual foram realizadas oito entrevistas semiestruturadas com profissionais de saúde pública no Peru entre maio e dezembro de 2020. Elas foram gravadas e transcritas para a coleta de informações. Resultados: os desafios organizacionais e de recursos foram destacados nas entrevistas realizadas, como a escassez de equipamentos de proteção individual e a gestão burocrática. O impacto humano e social foi destacado em termos de comunicação, empatia e reavaliação de valores pessoais. Adaptações significativas foram observadas na prática médica, com ênfase no manejo e treinamento dos pacientes. Conclusões: as experiências clínicas e emocionais refletiram os desafios e motivações dos profissionais de saúde durante a pandemia; em que foi demonstrada a necessidade de maior adaptabilidade e preparação, bem como a importância do bem-estar emocional e social
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Equipamento de Proteção IndividualRESUMO
La pandemia de COVID-19 representó un gran desafío para los profesionales de la salud de Perú, debido al riesgo elevado de contagio, la sobrecarga laboral y afectación de la salud mental. Objetivo: Describir las experiencias laborales y percepciones del personal sanitario de este país durante la pandemia de COVID-19. Materiales y Métodos: Se llevó a cabo un estudio cualitativo fenomenológico, en el que se realizaron ocho entrevistas semiestructuradas a profesionales de la salud pública en Perú entre mayo y diciembre del año 2020. Las mismas fueron grabadas y transcritas para la recolección de la información, las cuales se analizaron en categorías. Resultados: En las entrevistas realizadas se destacaron los desafíos organizativos y de recursos, como la escasez de equipos de protección personal y gestión burocrática. Se resaltó el impacto humano y social en términos de comunicación, empatía y reevaluación de valores personales. Se observó adaptaciones significativas en la práctica médica, con énfasis en el manejo de pacientes y la capacitación. Conclusiones: Las experiencias clínicas y emocionales reflejaron los retos y motivaciones del personal sanitario durante la pandemia; en el que se demostró la necesidad de una mayor adaptabilidad y preparación, así como la importancia del bienestar emocional y social.
The COVID-19 pandemic represented a great challenge for health professionals in Peru, due to the high risk of contagion, work overload, and impact on mental health. Objective: Describe the work experiences and perceptions of health personnel in this country during the COVID-19 pandemic. Materials and Methods: A qualitative phenomenological study was carried out, in which eight semi-structured interviews were carried out with public health professionals in Peru between May and December 2020. They were recorded and transcribed for the collection of information, which were analyzed in categories. Results: Organizational and resource challenges were highlighted in the interviews conducted, such as the shortage of personal protective equipment and bureaucratic management. The human and social impact was highlighted in terms of communication, empathy and reevaluation of personal values. Significant adaptations were observed in medical practice, with emphasis on patient management and training. Conclusions: The clinical and emotional experiences reflected the challenges and motivations of healthcare personnel during the pandemic; in which the need for greater adaptability and preparation was demonstrated, as well as the importance of emotional and social well-being.
A pandemia da COVID-19 representou um grande desafio para os profissionais de saúde no Peru, devido ao alto risco de contágio, sobrecarga de trabalho e impacto na saúde mental. Objetivo: Descrever as experiências de trabalho e percepções do pessoal de saúde neste país durante a pandemia da COVID-19. Materiais e Métodos: Foi realizado um estudo fenomenológico qualitativo, no qual foram realizadas oito entrevistas semiestruturadas com profissionais de saúde pública no Peru entre maio e dezembro de 2020. Elas foram gravadas e transcritas para a coleta de informações. Resultados: Os desafios organizacionais e de recursos foram destacados nas entrevistas realizadas, como a escassez de equipamentos de proteção individual e a gestão burocrática. O impacto humano e social foi destacado em termos de comunicação, empatia e reavaliação de valores pessoais. Adaptações significativas foram observadas na prática médica, com ênfase no manejo e treinamento dos pacientes. Conclusões: As experiências clínicas e emocionais refletiram os desafios e motivações dos profissionais de saúde durante a pandemia; em que foi demonstrada a necessidade de maior adaptabilidade e preparação, bem como a importância do bem-estar emocional e social.
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Proteins have evolved through mutations-amino acid substitutions-since life appeared on Earth, some 109 years ago. The study of these phenomena has been of particular significance because of their impact on protein stability, function, and structure. This study offers a new viewpoint on how the most recent findings in these areas can be used to explore the impact of mutations on protein sequence, stability, and evolvability. Preliminary results indicate that: (1) mutations can be viewed as sensitive probes to identify 'typos' in the amino-acid sequence, and also to assess the resistance of naturally occurring proteins to unwanted sequence alterations; (2) the presence of 'typos' in the amino acid sequence, rather than being an evolutionary obstacle, could promote faster evolvability and, in turn, increase the likelihood of higher protein stability; (3) the mutation site is far more important than the substituted amino acid in terms of the marginal stability changes of the protein, and (4) the unpredictability of protein evolution at the molecular level-by mutations-exists even in the absence of epistasis effects. Finally, the Darwinian concept of evolution "descent with modification" and experimental evidence endorse one of the results of this study, which suggests that some regions of any protein sequence are susceptible to mutations while others are not. This work contributes to our general understanding of protein responses to mutations and may spur significant progress in our efforts to develop methods to accurately forecast changes in protein stability, their propensity for metamorphism, and their ability to evolve.
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Evolução Molecular , Mutação , Estabilidade Proteica , Proteínas , Proteínas/genética , Proteínas/química , Proteínas/metabolismo , Sequência de AminoácidosRESUMO
The relationship between protein stability and functional evolution is little explored in proteins purified from natural sources. Here, we investigated a novel family of egg proteins (Perivitellin-1, PV1) from Pomacea snails. Their remarkable stability and clade-related functions in most derived clades (Canaliculata and Bridgesii) make them excellent candidates for exploring this issue. To that aim, we studied PV1 (PpaPV1) from the most basal lineage, Flagellata. PpaPV1 displays unparalleled structural and kinetic stability, surpassing PV1s from derived clades, ranking among the most hyperstable proteins documented in nature. Its spectral features contribute to a pale egg coloration, exhibiting a milder glycan binding lectin activity with a narrower specificity than PV1s from the closely related Bridgesii clade. These findings provide evidence for substantial structural and functional changes throughout the genus' PV1 evolution. We observed that structural and kinetic stability decreased in a clade-related fashion and was associated with large variations in defensive traits. For instance, pale PpaPV1 lectin turns potent in the Bridgesii clade, adversely affecting gut morphology, while giving rise to brightly colored PV1s providing eggs with a conspicuous, probably warning signal in the Canaliculata clade. This work provides a comprehensive comparative analysis of PV1s from various apple snail species within a phylogenetic framework, offering insights into the interplay among their structural features, stability profiles and functional roles. More broadly, our work provides one of the first examples from natural evolution showing the crucial link among protein structure, stability and evolution of new functions.