RESUMO
Four new species of armored scale insect, Clavaspis selvatica sp. nov., Clavaspis virolae sp. nov., Davidsonaspis tovomitae sp. nov., and Rungaspis neotropicalis sp. nov., are described and illustrated from Panama. We also transfer two previously described species of Panamanian Aspidiotini to new genera, Hemiberlesia crescentiae (Ferris) comb. nov. and Rungaspis rigida (Ferris) comb. nov., and report the first record of Selenaspidopsis browni Nakahara in Panama. A key to the species of Aspidiotini occurring in Panama is provided.
RESUMO
The scale insect Dactylopius coccus produces high amounts of carminic acid, which has historically been used as a pigment by pre-Hispanic American cultures. Nowadays carmine is found in food, cosmetics, and textiles. Metagenomic approaches revealed that Dactylopius spp. cochineals contain two Wolbachia strains, a betaproteobacterium named Candidatus Dactylopiibacterium carminicum and Spiroplasma, in addition to different fungi. We describe here a transcriptomic analysis indicating that Dactylopiibacterium is metabolically active inside the insect host, and estimate that there are over twice as many Dactylopiibacterium cells in the hemolymph than in the gut, with even fewer in the ovary. Albeit scarce, the transcripts in the ovaries support the presence of Dactylopiibacterium in this tissue and a vertical mode of transmission. In the cochineal, Dactylopiibacterium may catabolize plant polysaccharides, and be active in carbon and nitrogen provisioning through its degradative activity and by fixing nitrogen. In most insects, nitrogen-fixing bacteria are found in the gut, but in this study they are shown to occur in the hemolymph, probably delivering essential amino acids and riboflavin to the host from nitrogen substrates derived from nitrogen fixation.
RESUMO
In this study, two new species of soft scale (Hemiptera: Coccomorpha: Coccidae) associated with rosemary, Rosmarinus officinalis L. (Lamiaceae) from Colombia, Cryptinglisia corpoica Kondo Montes sp. nov. and Cryptinglisia ica Montes Kondo sp. nov. are described and illustrated based on the adult female. Two other coccid species, namely Parasaissetia nigra (Nietner) and Saissetia coffeae (Walker), are newly recorded on rosemary. An identification key is presented to all species of soft scale that have been reported on Rosmarinus spp. An updated key to soft scale insects of the genus Cryptinglisia Cockerell is provided also.
Assuntos
Hemípteros , Rosmarinus , Animais , Colômbia , Feminino , LamiaceaeRESUMO
Dactylopius species, known as cochineal insects, are the source of the carminic acid dye used worldwide. The presence of two Wolbachia strains in Dactylopius coccus from Mexico was revealed by PCR amplification of wsp and sequencing of 16S rRNA genes. A metagenome analysis recovered the genome sequences of Candidatus Wolbachia bourtzisii wDacA (supergroup A) and Candidatus Wolbachia pipientis wDacB (supergroup B). Genome read coverage, as well as 16S rRNA clone sequencing, revealed that wDacB was more abundant than wDacA. The strains shared similar predicted metabolic capabilities that are common to Wolbachia, including riboflavin, ubiquinone, and heme biosynthesis, but lacked other vitamin and cofactor biosynthesis as well as glycolysis, the oxidative pentose phosphate pathway, and sugar uptake systems. A complete tricarboxylic acid cycle and gluconeogenesis were predicted as well as limited amino acid biosynthesis. Uptake and catabolism of proline were evidenced in Dactylopius Wolbachia strains. Both strains possessed WO-like phage regions and type I and type IV secretion systems. Several efflux systems found suggested the existence of metal toxicity within their host. Besides already described putative virulence factors like ankyrin domain proteins, VlrC homologs, and patatin-like proteins, putative novel virulence factors related to those found in intracellular pathogens like Legionella and Mycobacterium are highlighted for the first time in Wolbachia Candidate genes identified in other Wolbachia that are likely involved in cytoplasmic incompatibility were found in wDacB but not in wDacA.
Assuntos
Genoma Bacteriano , Genômica , Hemípteros/microbiologia , Wolbachia/genética , Animais , Sistemas de Secreção Bacterianos/genética , Sistemas de Secreção Bacterianos/metabolismo , Transporte Biológico , Metabolismo Energético , Feminino , Variação Genética , Genômica/métodos , Sequenciamento de Nucleotídeos em Larga Escala , RNA Bacteriano , RNA Ribossômico 16S , Estresse Fisiológico/genética , Simbiose , Fatores de Virulência , Wolbachia/classificação , Wolbachia/isolamento & purificação , Wolbachia/metabolismoRESUMO
A new soft scale (Hemiptera: Coccoidea: Coccidae) species, Pulvinariacaballeroramosae Tanaka & Kondo, sp. n., is described from specimens collected on twigs of Ficussoatensis Dugand (Moraceae) in Bogota, Colombia. The new species resembles Pulvinariadrymiswinteri Kondo & Gullan, described from Chile on Drimyswinteri J.R. Forst. & G. Forst. (Winteraceae), but differs in the distribution of preopercular pores on the dorsum, the presence of dorsal tubular ducts, dorsal microducts, and reticulation on the anal plates; and in its feeding habits, i.e., Pulvinariacaballeroramosae feeds on the twigs whereas Pulvinariadrymiswinteri feeds on the leaves of its host. A key to the Colombian species of Pulvinaria Targioni Tozzetti is provided.
RESUMO
Scale insects (Hemiptera: Coccoidae) constitute a very diverse group of sap-feeding insects with a large diversity of symbiotic associations with bacteria. Here, we present the complete genome sequence, metabolic reconstruction, and comparative genomics of the flavobacterial endosymbiont of the giant scale insect Llaveia axin axin. The gene repertoire of its 309,299 bp genome was similar to that of other flavobacterial insect endosymbionts though not syntenic. According to its genetic content, essential amino acid biosynthesis is likely to be the flavobacterial endosymbiont's principal contribution to the symbiotic association with its insect host. We also report the presence of a γ-proteobacterial symbiont that may be involved in waste nitrogen recycling and also has amino acid biosynthetic capabilities that may provide metabolic precursors to the flavobacterial endosymbiont. We propose "Candidatus Walczuchella monophlebidarum" as the name of the flavobacterial endosymbiont of insects from the Monophlebidae family.
Assuntos
Flavobacterium/genética , Genoma Bacteriano , Hemípteros/microbiologia , Simbiose , Animais , Clonagem Molecular , DNA Bacteriano/genética , Flavobacterium/classificação , Genômica , Hibridização in Situ Fluorescente , Dados de Sequência Molecular , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNARESUMO
The infestation of Azadirachta indica A. Juss. (Meliaceae) by scale insects of the species Aonidiella orientalis (Newstead) (Hemiptera: Diaspididae) is reported for the first time. Leaves and twigs exhibiting signs of wilting were collected from the basal-interior parts of the canopy of neem trees that had been planted in the city of Limoeiro do Norte, located in the northeastern state of Ceará, Brazil. The identity of the oriental scale insect A. orientalis was confirmed by microscopic examination. The insect pest has not been observed previously in the northern states of Brazil, hence the present report is significant in that it focuses attention on the mobility of A. orientalis and on its potential to infest other plant species in that region.
A infestação de Azadiractha indica A. Juss. (Meliaceae) pela cochonilha da espécie Aonidiella orientalis (Newstead) (Hemiptera: Diaspididae) é relatada pela primeira vez. Folhas e galhos exibindo sinais de murcha foram obtidos da parte basal interna da copa de árvores que haviam sido plantadas na cidade de Limoeiro do Norte localizada no estado do Ceará, nordeste do Brasil. A identidade dos insetos foi confirmada através de exame microscópico. Visto que esse inseto não havia sido observado antes nos estados do norte do Brasil, o presente artigo é importante, pois desperta a atenção para a mobilidade de A. orientalis e seu potencial para infestar outras espécies nesta região.
Assuntos
Meliaceae , Azadirachta , Hemípteros , InsetosRESUMO
A new species of Hemilecanium Newstead, Hemilecanium guanabana Kondo & Hodgson n. sp., is described and illustrated based on the adult female, adult male and first instar. The specimens were collected in the municipality of Palmira, state of Valle del Cauca, Colombia, on soursop, Annona muricata (Annonaceae). Updated identification keys are provided for the adult females of all 28 species of the genus Hemilecanium, and for known adult males and first instars. An updated list of the 23 species of soft scales (Coccidae) known from soursop worldwide is included.
Assuntos
Hemípteros , Distribuição Animal , Animais , Annona , Annonaceae , Feminino , Hemípteros/anatomia & histologia , Hemípteros/classificação , MasculinoRESUMO
The species of Termitococcinae are revised. This subfamily, restricted to the Neotropical region, is composed of five valid species included in two genera: Termitococcus aster Silvestri, 1901, Termitococcus carratoi Silvestri, 1936, Eurhizococcus brasiliensis (Wille, 1922), Eurhizococcus brevicornis (Silvestri, 1901), and Eurhizococcus colombianus Jakubski, 1965. The present study provides the redescription of these species, including the morphological characterization of the three stages (crawlers, cyst and adult female). Identification keys and illustrations for the species are also included. Lectotypes and paralectotypes of two species are designated.
As espécies de Termitococcinae são revisadas. Esta subfamília, restrita à Região Neotropical, é composta de cinco espécies válidas, incluídas em dois gêneros: Termitococcus aster Silvestri, 1901, Termitococcus carratoi Silvestri, 1936, Eurhizococcus brasiliensis (Wille, 1922), Eurhizococcus brevicornis (Silvestri, 1901) e Eurhizococcus colombianus Jakubski, 1965. O estudo traz a redescrição das espécies da subfamília, incluindo a caracterização morfológica dos três estágios de desenvolvimento (ninfa ambulatória, cisto e fêmea adulta). Chaves de identificação e ilustrações são também incluídas. São designados lectótipos e paralectótipos para duas espécies.
RESUMO
The species of Termitococcinae are revised. This subfamily, restricted to the Neotropical region, is composed of five valid species included in two genera: Termitococcus aster Silvestri, 1901, Termitococcus carratoi Silvestri, 1936, Eurhizococcus brasiliensis (Wille, 1922), Eurhizococcus brevicornis (Silvestri, 1901), and Eurhizococcus colombianus Jakubski, 1965. The present study provides the redescription of these species, including the morphological characterization of the three stages (crawlers, cyst and adult female). Identification keys and illustrations for the species are also included. Lectotypes and paralectotypes of two species are designated.
As espécies de Termitococcinae são revisadas. Esta subfamília, restrita à Região Neotropical, é composta de cinco espécies válidas, incluídas em dois gêneros: Termitococcus aster Silvestri, 1901, Termitococcus carratoi Silvestri, 1936, Eurhizococcus brasiliensis (Wille, 1922), Eurhizococcus brevicornis (Silvestri, 1901) e Eurhizococcus colombianus Jakubski, 1965. O estudo traz a redescrição das espécies da subfamília, incluindo a caracterização morfológica dos três estágios de desenvolvimento (ninfa ambulatória, cisto e fêmea adulta). Chaves de identificação e ilustrações são também incluídas. São designados lectótipos e paralectótipos para duas espécies.