RESUMO
Valerian extract is used in complementary and alternative medicine for its anxiolytic and sedative properties. Our previous research demonstrated valerian interactions with glutamate receptors. The purpose of this study was to determine if valerian anxiolytic properties are mediated by metabotropic glutamate receptors (mGluR) such as mGluR (1/5) (mGluR I) and mGluR (2/3) (mGluR II). Adult wild-type zebrafish (Danio rerio) prefer the black compartment and avoid the white compartment in the dark/light preference task. Zebrafish exposed to 1 mg/mL of valerian extract or 0.00117 mg/mL valerenic acid increased their residence time in the white side by 84.61 ± 6.55 % and 58.30 ± 8.97 %, respectively. LAP3 (mGluR I antagonist) and EGLU (mGluR II antagonist) significantly inhibited the effects of valerian and valerenic acid. These results demonstrated that valerian and valerenic acid have anxiolytic properties in the zebrafish. Moreover, the selective interaction of valerian with mGluR I and II represent an alternative explanation for the anxiolytic properties of this plant and support the role of mGluR in anxiety.
Assuntos
Ansiolíticos/farmacologia , Receptores de Glutamato Metabotrópico/antagonistas & inibidores , Valeriana/química , Peixe-Zebra/metabolismo , Animais , Ansiolíticos/química , Ansiedade , Comportamento Animal/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão , Escuridão , Feminino , Indenos/química , Indenos/farmacologia , Luz , Masculino , Fitoterapia , Raízes de Plantas/química , Ácido Quisquálico/farmacologia , Receptores de Glutamato Metabotrópico/agonistas , Sesquiterpenos/química , Sesquiterpenos/farmacologia , Fatores de TempoRESUMO
Choline acetyltransferase (ChAT) activity was reduced by more than 85% in cultured retina cells after 16 h treatment with 150 microM kainate (T(1/2) : 3.5 h). Glutamate, AMPA and quisqualate also inhibited the enzyme in equivalent proportion. Cell lesion measured by lactate dehydrogenase (LDH) release, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide - thiazolyl blue (MTT) reduction and microscopic observation was not detected even after 48 h with kainate. Other retina neurochemical markers were not affected by kainate and full recovery of the enzyme was achieved 9 days after kainate removal. Moreover, hemicolinium-3 sensitive choline uptake and hemicolinium-3 binding sites were maintained intact after kainate treatment. The immunoblot and immunohistochemical analysis of the enzyme revealed that ChAT molecules were maintained in cholinergic neurons. The use of antagonists showed that ionotropic and group 1 metabotropic receptors mediated the effect of glutamate on ChAT inhibition, in a calcium dependent manner. The quisqualate mediated ChAT inhibition and part of the kainate effect (30%) was prevented by 5 mM N(G)-nitro-L-arginine methyl ester (L-NAME). Veratridine (3 microM) also reduced ChAT by a Ca(2+) dependent, but glutamate independent mechanism and was prevented by 1 microM tetrodotoxin.
Assuntos
Colina O-Acetiltransferase/antagonistas & inibidores , Agonistas de Aminoácidos Excitatórios/farmacologia , Antagonistas de Aminoácidos Excitatórios/farmacologia , Ácido Caínico/análogos & derivados , Neurônios/efeitos dos fármacos , Retina/efeitos dos fármacos , Animais , Cálcio/metabolismo , Células Cultivadas , Embrião de Galinha , Cicloleucina/análogos & derivados , Cicloleucina/farmacologia , Inibidores Enzimáticos/farmacologia , Ácido Glutâmico/farmacologia , Ácido Caínico/farmacologia , NG-Nitroarginina Metil Éster/farmacologia , Neurônios/citologia , Neurônios/fisiologia , Propionatos/farmacologia , Ácido Quisquálico/farmacologia , Retina/citologia , Retina/fisiologia , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/fisiologia , Tetrodotoxina/farmacologia , Trifluoperazina/farmacologia , Verapamil/farmacologia , Ácido alfa-Amino-3-hidroxi-5-metil-4-isoxazol Propiônico/farmacologia , Ácido gama-Aminobutírico/metabolismoRESUMO
Glutamate stimulated release of [3H]GABA was studied, during receptor desensitization block and its modulation by voltage gated Ca2+ channels, internal Ca2+ mobilization and GABA transport inhibitors from olfactory bulb slices. Under control conditions, glutamate and agonists induced release was strongly inhibited by Mg/0 Ca2+ Krebs and Cd2+ and partially inhibited by Ni2+ and nifedipine. Cyclothiazide, which blocks desensitization of glutamate receptors, potentiated glutamate, kainate, AMPA and quisqualate induced release. This effect was less dependent of entry of external Ca2+, but was inhibited by trifluoperazine and thapsigargin, inhibitors of Ca2+-calmodulin and endoplasmatic Ca2+ ATPase respectively. Nipecotic acid and NO-711, inhibitors of the GABA transporter, were also able to reduce cyclothiazide potentiated release induced by the 4 secretagogues. Under control conditions, glutamate stimulates the release of GABA in cooperation with VDCC. However, during receptor desensitization block, glutamate stimulated GABA release is mainly modulated through mechanisms dependent on internal Ca2+ mobilization and reversal of the GABA transporter.
Assuntos
Ácido Glutâmico/farmacologia , Neurônios/fisiologia , Bulbo Olfatório/fisiologia , Receptores de Glutamato/fisiologia , Ácido gama-Aminobutírico/metabolismo , Animais , Cálcio/farmacologia , Técnicas In Vitro , Cinética , Masculino , N-Metilaspartato/farmacologia , Neurônios/citologia , Neurônios/efeitos dos fármacos , Bulbo Olfatório/citologia , Cloreto de Potássio/farmacologia , Ácido Quisquálico/farmacologia , Ratos , Ratos Sprague-Dawley , Ácido alfa-Amino-3-hidroxi-5-metil-4-isoxazol Propiônico/farmacologiaRESUMO
Activation of glutamate receptors has been implicated in excitotoxicity. Here, we have investigated whether subtoxic concentrations of glutamate can modulate neuronal death in the developing retina. Explants of rat retinas were pre-incubated with glutamate, N-methyl-d-aspartate (NMDA), kainate, quisqualate or trans-1-amino-1,3-cyclopentanedicarboxylic acid (t-ACPD) for 18 h. Then, glutamate (6 mM) was added to the explants for an additional 6 h. Glutamate-induced degeneration was restricted to the emerging inner nuclear layer. Pre-incubation with glutamate, NMDA, or both, reduced glutamate-induced neuronal death and protected against neuronal death induced by irradiation (2 Gy). The NMDA receptor antagonists, 2-amino-5-phosphonovaleric acid (d-APV; 30 microM) or 5-methyl-10,11-dihydro-5H-dibenzocyclohepten-5,10-imine hydrogen maleate (MK-801; 30 microM), prevented glutamate-induced neuroprotection. To investigate whether this neuroprotection was mediated by neurotrophins, we incubated retinal explants with either brain-derived neurotrophic factor or neurotrophin-4. Both treatments resulted in partial protection against glutamate-induced neurotoxicity. Furthermore, NMDA mediated neuroprotection was totally reversed when a soluble form of the specific tyrosine kinase receptor B was simultaneously added to the explants. Our results suggest that activation of NMDA receptors may control neuronal death in the retina during development. This modulation seems to depend, at least in part, on the release of neurotrophins within the retina.
Assuntos
Fator Neurotrófico Derivado do Encéfalo/farmacologia , Ácido Glutâmico/toxicidade , Fatores de Crescimento Neural/fisiologia , Receptores de N-Metil-D-Aspartato/fisiologia , Retina/citologia , 2-Amino-5-fosfonovalerato/farmacologia , Animais , Apoptose/efeitos dos fármacos , Apoptose/efeitos da radiação , Cicloleucina/análogos & derivados , Cicloleucina/farmacologia , Maleato de Dizocilpina/farmacologia , Agonistas de Aminoácidos Excitatórios/farmacologia , Antagonistas de Aminoácidos Excitatórios/farmacologia , Ácido Caínico/farmacologia , N-Metilaspartato/farmacologia , Fármacos Neuroprotetores/farmacologia , Quinoxalinas/farmacologia , Ácido Quisquálico/farmacologia , Ratos , Ratos Endogâmicos , Receptores Proteína Tirosina Quinases/metabolismo , Receptor do Fator Neutrófico Ciliar , Receptores de Fator de Crescimento Neural/metabolismo , Retina/efeitos dos fármacosRESUMO
The purpose of the present study was to examine the in vitro effect of L-glutamate and its agonists on basal and potassium-evoked GABA release from incubated mediobasal hypothalamus (MBH) of intact, ovariectomized (OVX) and OVX-estrogenized female rats. L-glutamate (100 microM) decreased evoked GABA release from MBH of intact female rats in diestrus. NMDA and quisqualate (10 and 100 microM) modified neither basal nor evoked hypothalamic GABA release of intact rats. However, kainate (10 and 100 microM) decreased hypothalamic basal and evoked GABA release of intact rats. Kainate induced no changes in basal or in evoked GABA release from hypothalami of OVX rats, but decreased GABA release in chronically estrogenized rats. DNQX (6,7-dinitroquinoxaline-2,3-dione), a non-NMDA receptor antagonist, failed to affect GABA release but blocked the inhibitory effect of kainate. The kainate effect was not Mg2+-sensitive and was not inhibited by D-AP5 (D(-)-2-amino-5-phosphonopentanoic acid), an NMDA-specific receptor antagonist. Kainate induced no changes in nitric oxide synthase activity in MBH of either intact or estrogenized rats. These data indicate that kainate decreases GABA release from MBH of female rats through a non-NMDA receptor subtype, and provide evidence to support the view that kainate-mediated decrease of the hypothalamic GABAergic tone is affected by estrogens.
Assuntos
Aminoácidos Excitatórios/farmacologia , Ácido Glutâmico/farmacologia , Hipotálamo/efeitos dos fármacos , Ácido gama-Aminobutírico/metabolismo , Animais , Depressão Química , Diestro , Implantes de Medicamento , Estradiol/administração & dosagem , Estradiol/farmacologia , Agonistas de Aminoácidos Excitatórios/farmacologia , Antagonistas de Aminoácidos Excitatórios/farmacologia , Feminino , Hipotálamo/metabolismo , Interneurônios/citologia , Interneurônios/efeitos dos fármacos , Ácido Caínico/farmacologia , N-Metilaspartato/farmacologia , Proteínas do Tecido Nervoso/metabolismo , Óxido Nítrico Sintase/metabolismo , Ovariectomia , Quinoxalinas/farmacologia , Ácido Quisquálico/farmacologia , Ratos , Ratos Wistar , Receptores de Glutamato/efeitos dos fármacos , Receptores de Glutamato/fisiologia , Receptores de N-Metil-D-Aspartato/efeitos dos fármacos , Receptores de N-Metil-D-Aspartato/fisiologiaRESUMO
Metabotropic glutamate receptors (mGluRs) have been shown to modulate adenylate cyclase activity via G-proteins. In the present study we report similar results to the previously observed in the literature, showing that glutamate and the metabotropic agonists, 1S,3R-ACPD or quisqualate induced cAMP accumulation in hippocampal slices of young rats. Moreover, guanine nucleotides GTP, GDP or GMP, inhibited the glutamate-induced cAMP accumulation. By measuring LDH activity in the buffer surrounding the slices, we showed that the integrity of the slices was maintained, indicating that the effect of guanine nucleotides was extracellular. GMP, GDPbeta-S or Gpp(NH)p abolished quisqualate-induced cAMP accumulation. GDPbeta-S or Gpp(NH)p but not GMP inhibited 1S,3R-ACPD-induced cAMP accumulation. The response evoked by glutamate was also abolished by the mGluR antagonists: L-AP3 abolished glutamate-induced cAMP accumulation in a dose-dependent manner and MCPG was effective only at the 2 mM dose. DNQX was ineffective. We are reporting here, an inhibition induced by guanine nucleotides, via an extracellular site (s), similar to the observed with classical glutamate antagonists on a cellular response evoked by mGluR agonists.
Assuntos
AMP Cíclico/antagonistas & inibidores , Nucleotídeos de Guanina/farmacologia , Receptores de Glutamato Metabotrópico/metabolismo , Animais , AMP Cíclico/metabolismo , Cicloleucina/análogos & derivados , Cicloleucina/farmacologia , Ácido Glutâmico/farmacologia , Guanosina Difosfato/análogos & derivados , Guanosina Difosfato/farmacologia , Guanosina Monofosfato/farmacologia , Guanosina Trifosfato/farmacologia , Guanilil Imidodifosfato/farmacologia , Hipocampo/efeitos dos fármacos , Hipocampo/crescimento & desenvolvimento , Hipocampo/metabolismo , Neurotoxinas/farmacologia , Ácido Quisquálico/farmacologia , Ratos , Ratos Wistar , Tionucleotídeos/farmacologiaRESUMO
Postsynaptic densities (PSDs) were isolated from rat brain cortex and hippocampus, purified and incorporated into giant (5-80 microns in diameter) liposomes. Gigaohm seals were obtained with a patch-clamp pipette, and a giant liposome PSD-containing membrane patch, was excised and recorded. The PSD was always oriented in an inside-out configuration. This allowed receptor agonists or antagonists to be added from the interior of the recording pipette, and also the addition of different substances, such as ATP, calcium, calmodulin and others to the 'intracellular' side of the PSD, i.e. to the bath. alpha-Amino-3-hydroxy-5-methylisoxazole propionic acid (AMPA) receptor agonists such as quisqualate or AMPA induced in the PSD a complex pattern of electrical activity, that was blocked by 10 microM 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), but not by 2-aminophosphonovalerate (APV). The currents generated by 0.5-1 microM quisqualate were increased by about 100% when the PSDs were phosphorylated. Similar findings were obtained when the agonist was 0.2-2 microM kainate. These currents were also blocked by a non-N-methyl-D-aspartate (NMDA) receptor antagonist but not by APV, and were increased by about 70% by phosphorylation of the PSDs. Addition of 5-10 microM NMDA plus 1 microM glycine to the 'extracellular' side of the PSD, led to a characteristic pattern of activity, with the opening of multiple receptor ion channels. This was entirely blocked by 10 microM APV. Addition of extracellular Mg2+ (1-2 mM) induced a voltage-dependent block of the currents. Phosphorylation of the PSD led to an increase of Mg(2+)-blocked current of about 80%. The effect of phosphorylation on ion channel activity showed a markedly different requirement for calcium and for calmodulin among the AMPA, kainate and NMDA types of glutamate receptors, thus suggesting that each receptor type is coupled at the synapse with a unique complement of protein phosphokinases.
Assuntos
Córtex Cerebral/metabolismo , Hipocampo/metabolismo , Proteínas Quinases/metabolismo , Receptores de Glutamato/metabolismo , Sinapses/química , 2-Amino-5-fosfonovalerato/farmacologia , 6-Ciano-7-nitroquinoxalina-2,3-diona/farmacologia , Trifosfato de Adenosina/farmacologia , Animais , Cálcio/farmacologia , Calmodulina/farmacologia , Córtex Cerebral/química , Ácido Egtázico/farmacologia , Estimulação Elétrica , Agonistas de Aminoácidos Excitatórios/farmacologia , Antagonistas de Aminoácidos Excitatórios/farmacologia , Glicina/farmacologia , Hipocampo/química , Ácido Caínico/farmacologia , Potenciação de Longa Duração/fisiologia , Magnésio/farmacologia , N-Metilaspartato/farmacologia , Técnicas de Patch-Clamp , Fosforilação , Ácido Quisquálico/farmacologia , Ratos , Sinapses/efeitos dos fármacos , Sinapses/enzimologiaRESUMO
This study was carried out to investigate possible interactions between some glutamatergic agonists and the peptide alpha-MSH upon the cyclic AMP levels. We used an in vitro tissue slice preparation incubated in the presence of different glutamatergic agonists such as N-methyl-D-aspartic acid (NMDA), quisqualic acid (QUIS), kainic acid (KA), and the peptide alpha-MSH together with each agonist. Slices containing caudate putamen and accumbens were chosen according to neurochemical data indicating that the striatum contains a moderate amount of MSH binding sites and also receives glutamatergic innervation. Exposure of these slices to either MSH or to the agonists NMDA or QUIS resulted in an increase in the cAMP levels in relation to controls. Nevertheless, incubation with KA resulted in no changes in the nucleotide levels. The combination of MSH/NMDA induced a reduction of cAMP levels in relation to those obtained with NMDA alone. The combinations of QUIS/MSH or KA/MSH also induced variations in the values of nucleotide in relation to the those obtained with the peptide alone or with the corresponding agonist; these changes were related to the dose of agonist used in each case. The results obtained in these experiments suggest the existence of some interaction between the peptide and the agonist used.
Assuntos
AMP Cíclico/metabolismo , Agonistas de Aminoácidos Excitatórios/farmacologia , Ácido Caínico/farmacologia , N-Metilaspartato/farmacologia , Ácido Quisquálico/farmacologia , alfa-MSH/farmacologia , Animais , Núcleo Caudado/efeitos dos fármacos , Núcleo Caudado/metabolismo , Interações Medicamentosas , Técnicas In Vitro , Masculino , Núcleo Accumbens/efeitos dos fármacos , Núcleo Accumbens/metabolismo , Putamen/efeitos dos fármacos , Putamen/metabolismo , Ratos , Ratos WistarRESUMO
Phosphorylation of the astrocyte cell marker glial fibrillary acidic protein (GFAP) in hippocampal slices from immature rats (10-16 days postnatal) was strongly stimulated by glutamate in the presence of Ca2+. This effect apparently occurred via a metabotropic receptor since the specific agonist of metabotropic glutamate receptors, 1S,3R-1-aminocyclopentane-1,3-dicarboxylic acid (1S,3R-ACPD), stimulated GFAP phosphorylation by 173% whilst the mixed agonists, ibotenate and quisqualate, stimulated to a lesser extent. Ionotropic agonists were mainly ineffective. The action of 1S,3R-ACPD was blocked by L(+)-2-amino-3-phosphonopropionic acid (L-AP3) a specific antagonist of the metabotropic glutamate receptor coupled to the hydrolysis of phosphoinositides and was reduced by 70% by preincubation of the slices with pertussis toxin. In contrast to these results with immature animals glutamate had little or no effect on the phosphorylation of GFAP in hippocampal slices from adult rats.
Assuntos
Agonistas de Aminoácidos Excitatórios/farmacologia , Proteína Glial Fibrilar Ácida/metabolismo , Ácido Glutâmico/farmacologia , Hipocampo/metabolismo , Receptores de Glutamato Metabotrópico/fisiologia , Animais , Autorradiografia , Cicloleucina/análogos & derivados , Cicloleucina/farmacologia , Eletroforese em Gel Bidimensional , Feminino , Proteína Glial Fibrilar Ácida/isolamento & purificação , Hipocampo/efeitos dos fármacos , Ácido Ibotênico/farmacologia , Técnicas In Vitro , Cinética , Masculino , Neurotoxinas/farmacologia , Fosfatos/metabolismo , Fosfoproteínas/isolamento & purificação , Fosfoproteínas/metabolismo , Fosforilação , Ácido Quisquálico/farmacologia , Ratos , Ratos Wistar , Receptores de Glutamato Metabotrópico/efeitos dos fármacosRESUMO
The presence of kainic (KA) and quisqualic acid (QA) receptors on inner retinal neurones of the axolotl Ambystoma mexicanum has been studied using intracellular recording techniques. In the presence of CoCl2, which blocks neurotransmitter release, KA and QA depolarized the membrane. The minimum concentration of KA that induced a response was 1 microM and a maximum response was obtained with 10 microM (EC50: 3 microM). The operating range of QA was between 0.5 and 5 microM with an EC50 of 1 microM. These data show that inner retinal cells of the axolotl are sensitive to KA and QA. Cis-2,3-piperidine dicarboxylic acid (PDA, 3 mM) completely blocked responses to 5 microM KA, but not those induced by 2 microM QA. This suggests that the KA- and QA-sensitive receptors on inner retinal cells of the salamander are pharmacologically different and that PDA can be a valuable tool in distinguishing KA- and QA-sensitive receptors on these neurones.