RESUMO
The goal of this study was to report the occurrence and histopathological aspects of uterine adenomyosis in bitches and queens. The sample consisted of 200 uteri from bitches (n =100) and queens (n = 100) obtained from elective ovarian-salpingohysterectomies. The animals were classified according to age, having given birth, and contraceptive use. Samples of the uterine horns were collected following routine histological processing using slides stained with hematoxylin and eosin. The data obtained were analysed using the chi-square test, with 5% significance level. Uterine adenomyosis was confirmed in 12% of bitches and 3% of queens. Age had no influence on the occurrence of this disease; however, not having given birth and the use of contraceptives favoured its appearance in bitches. Microscopically, portions of endometrial glands and stroma were present in the circular and vascular myometrial strata, with normal and cystic characteristics and variations in the glandular epithelium. There was cystic-type luminal content, such as calcification, hyaline substance, polyps, and mucin. This study made it possible to conclude that uterine adenomyosis occurs more frequently in bitches than in queens, regardless of age groups. Nulliparous bitches using contraceptives were the most affected. This pathology showed variations with respect to location and histopathological aspects, with the cystic type exhibiting calcification, hyaline substance, polyps, and mucin inside the lumen. These findings are being reported for the first time in the veterinary literature.
Objetivou-se com essa pesquisa relatar a ocorrência e os aspectos histopatológicos da adenomiose uterina em cadelas e gatas. Foram utilizados 200 úteros, de cadelas (n=100) e gatas (n=100), procedentes de ovariosalpingohisterectomias eletivas. Os animais foram classificados segundo a faixa etária, parição e uso de contraceptivos. Amostras dos cornos uterinos foram coletadas, seguindo-se ao processamento histológico de rotina, sendo as lâminas coradas pela hematoxilina e eosina. Os dados obtidos foram analisados pelo teste Qui-quadrado, com significância de 5%. A adenomiose uterina foi identificada em 12% das cadelas e 3% das gatas. Não houve influência na ocorrência dessa enfermidade quanto a faixa etária, porém a ausência de parição e o uso de contraceptivos favoreceram o aparecimento em cadelas. Microscopicamente, porções de glândulas e estroma endometrial estavam presentes no estrato miometrial circular e vascular, ocorrendo o tipo normal, cístico e variações no epitélio glandular. Houve presença de conteúdo luminal no tipo cístico, como calcificação, substância hialina, pólipos e mucina. Com esse estudo foi possível concluir que a adenomiose uterina ocorre com maior frequência em cadelas do que em gatas, independente da faixa etária. Cadelas nulíparas que utilizaram contraceptivos foram as mais afetadas. Essa patologia mostrou variações quanto a localização e aspecto histopatológico, exibindo no tipo cístico, calcificação, substância hialina, pólipos e mucina no interior do lúmen, sendo estes achados relatados pela primeira vez na literatura veterinária.
Assuntos
Feminino , Animais , Gatos , Cães , Adenomiose/epidemiologia , Adenomiose/fisiopatologia , Adenomiose/veterinária , Doenças Uterinas/complicações , Doenças Uterinas/veterinária , Útero/anatomia & histologia , Histerectomia/veterináriaRESUMO
The goal of this study was to report the occurrence and histopathological aspects of uterine adenomyosis in bitches and queens. The sample consisted of 200 uteri from bitches (n =100) and queens (n = 100) obtained from elective ovarian-salpingohysterectomies. The animals were classified according to age, having given birth, and contraceptive use. Samples of the uterine horns were collected following routine histological processing using slides stained with hematoxylin and eosin. The data obtained were analysed using the chi-square test, with 5% significance level. Uterine adenomyosis was confirmed in 12% of bitches and 3% of queens. Age had no influence on the occurrence of this disease; however, not having given birth and the use of contraceptives favoured its appearance in bitches. Microscopically, portions of endometrial glands and stroma were present in the circular and vascular myometrial strata, with normal and cystic characteristics and variations in the glandular epithelium. There was cystic-type luminal content, such as calcification, hyaline substance, polyps, and mucin. This study made it possible to conclude that uterine adenomyosis occurs more frequently in bitches than in queens, regardless of age groups. Nulliparous bitches using contraceptives were the most affected. This pathology showed variations with respect to location and histopathological aspects, with the cystic type exhibiting calcification, hyaline substance, polyps, and mucin inside the lumen. These findings are being reported for the first time in the veterinary literature.(AU)
Objetivou-se com essa pesquisa relatar a ocorrência e os aspectos histopatológicos da adenomiose uterina em cadelas e gatas. Foram utilizados 200 úteros, de cadelas (n=100) e gatas (n=100), procedentes de ovariosalpingohisterectomias eletivas. Os animais foram classificados segundo a faixa etária, parição e uso de contraceptivos. Amostras dos cornos uterinos foram coletadas, seguindo-se ao processamento histológico de rotina, sendo as lâminas coradas pela hematoxilina e eosina. Os dados obtidos foram analisados pelo teste Qui-quadrado, com significância de 5%. A adenomiose uterina foi identificada em 12% das cadelas e 3% das gatas. Não houve influência na ocorrência dessa enfermidade quanto a faixa etária, porém a ausência de parição e o uso de contraceptivos favoreceram o aparecimento em cadelas. Microscopicamente, porções de glândulas e estroma endometrial estavam presentes no estrato miometrial circular e vascular, ocorrendo o tipo normal, cístico e variações no epitélio glandular. Houve presença de conteúdo luminal no tipo cístico, como calcificação, substância hialina, pólipos e mucina. Com esse estudo foi possível concluir que a adenomiose uterina ocorre com maior frequência em cadelas do que em gatas, independente da faixa etária. Cadelas nulíparas que utilizaram contraceptivos foram as mais afetadas. Essa patologia mostrou variações quanto a localização e aspecto histopatológico, exibindo no tipo cístico, calcificação, substância hialina, pólipos e mucina no interior do lúmen, sendo estes achados relatados pela primeira vez na literatura veterinária.(AU)
Assuntos
Animais , Feminino , Gatos , Cães , Adenomiose/epidemiologia , Adenomiose/fisiopatologia , Adenomiose/veterinária , Útero/anatomia & histologia , Doenças Uterinas/complicações , Doenças Uterinas/veterinária , Histerectomia/veterináriaRESUMO
Background: Adenomyosis is a non-neoplastic condition, characterized by the presence of endometrial glands or cells in the myometrium. Adenomyosis is defined by post-operative microscopic examination of uterine tissue and a rarely seen infertility problem in bitches. The process of transformation of healthy uterus into adenomiotic uterus is still a mystery. We aimed to determine role of ovarian pathologies and uterine inflammations on pathogenesis of uterine adenomyosis in bitches.Cases: After ovariohysterectomy, the uteri were fixed and processed by routine methods. Sections were stained with HematoxylinEosin, Massons trichrome and Periodic-Acid-Schiff and immunohistochemically; with p53 protein, Ki-67, α-smooth-muscle actin, cytokeratin-CK 8, 14, 19 antibodies, estrogen and progesterone receptors by Avidin-Biotin-Complex-Peroxidase method. Histopathologically, 7 of the collected tissue samples (n = 38) were diagnosed as adenomyosis. There were unilateral (n = 6) or bilateral (n = 1) adenomyosis in which endometrial glands had broken through the myometrium, and in some cases there were fibromyoma, inflammation, papillary hyperplasia and cysts in glands. Vaginal fibroma, fibromyoma and benign mix tumor (n = 1), simple carcinoma (n = 3), carcinosarcoma (n = 3) in mammary gland were determinated histopathologically. In addition to these findings, follicular cysts (n = 4), parovarian cyst (n = 1), adenocarcinoma and Rete ovarii adenoma were diagnosed in some ovaries. The CK 19 was scored at endometrial glands (n = 2) positively and also, muscle fibers were stained positively with α-smooth-muscle actin antibody (n = 7).Discussion: Adenomyosis is an infertility problem in adult dogs and is hard to detect with routine gynecological examinations. Adenomyosis can be seen unaccompanied by other gynecological problems such as endometritis or leiomyomas, while mammary tumors with adenomyosis have been previously reported in women and in only one case of a bitch.[...](AU)
Assuntos
Animais , Feminino , Cães , Adenomiose/veterinária , Doenças Uterinas/complicações , Doenças Uterinas/veterinária , Doenças Ovarianas/complicações , Doenças Ovarianas/veterináriaRESUMO
Background: Adenomyosis is a non-neoplastic condition, characterized by the presence of endometrial glands or cells in the myometrium. Adenomyosis is defined by post-operative microscopic examination of uterine tissue and a rarely seen infertility problem in bitches. The process of transformation of healthy uterus into adenomiotic uterus is still a mystery. We aimed to determine role of ovarian pathologies and uterine inflammations on pathogenesis of uterine adenomyosis in bitches.Cases: After ovariohysterectomy, the uteri were fixed and processed by routine methods. Sections were stained with HematoxylinEosin, Massons trichrome and Periodic-Acid-Schiff and immunohistochemically; with p53 protein, Ki-67, α-smooth-muscle actin, cytokeratin-CK 8, 14, 19 antibodies, estrogen and progesterone receptors by Avidin-Biotin-Complex-Peroxidase method. Histopathologically, 7 of the collected tissue samples (n = 38) were diagnosed as adenomyosis. There were unilateral (n = 6) or bilateral (n = 1) adenomyosis in which endometrial glands had broken through the myometrium, and in some cases there were fibromyoma, inflammation, papillary hyperplasia and cysts in glands. Vaginal fibroma, fibromyoma and benign mix tumor (n = 1), simple carcinoma (n = 3), carcinosarcoma (n = 3) in mammary gland were determinated histopathologically. In addition to these findings, follicular cysts (n = 4), parovarian cyst (n = 1), adenocarcinoma and Rete ovarii adenoma were diagnosed in some ovaries. The CK 19 was scored at endometrial glands (n = 2) positively and also, muscle fibers were stained positively with α-smooth-muscle actin antibody (n = 7).Discussion: Adenomyosis is an infertility problem in adult dogs and is hard to detect with routine gynecological examinations. Adenomyosis can be seen unaccompanied by other gynecological problems such as endometritis or leiomyomas, while mammary tumors with adenomyosis have been previously reported in women and in only one case of a bitch.[...]
Assuntos
Feminino , Animais , Cães , Adenomiose/veterinária , Doenças Ovarianas/complicações , Doenças Ovarianas/veterinária , Doenças Uterinas/complicações , Doenças Uterinas/veterináriaRESUMO
Adenomyosis, a non-neoplastic myometrial proliferation, is a rare pathologic condition in domestic animals, of unknown etiology. There is a lack of information about the clinical and pathological features of this disease in cats, therefore just a few reports in that species. The study describes a case of adenomyosis in a feline female. A cat, six years old, undefined breed, not spayed, had abdominal swelling history and vaginal discharge. Clinically, upon abdominal palpation, a diffuse increase suggesting a uterine change was found. The vulva had sanguine-purulent exudates. The choice was for the neutering surgical. Uterine macroscopy revealed cystic endometrial hyperplasia/pyometra in uterine horns, and the presence of a diffuse mass on uterine corpus. Fragments of the mass were sent for histopathological analysis, which revealed adenomyosis of the uterine corpus. The patient exhibited adequate post-operative recovery. The adenomyosis should be considered as a differential diagnosis of uterine neoformation in domestic cats.(AU)