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Essential oils show several biological properties, such as antimicrobial activity, but have limitations regarding their availability and stability. To maximize their antimicrobial effect and protection against environmental conditions, Pickering-type emulsions were used to vehiculate oregano essential oil (OEO) using cellulose nanofibers (CNF) as emulsion stabilizer. Enzymatic hydrolysis was used to produce CNF from a food industry waste (cassava peel), obtaining an environmentally sustainable emulsion stabilizer. It was evaluated how the different properties of the nanofibers affected the stability of the emulsions. Furthermore, the composition of the dispersed phase was varied (different ratios of OEO and sunflower oil-SO) in view of the target application in biodegradable active coatings. Even at very low concentration (0.01 % w/w), CNF was able to form kinetically stable emulsions with small droplet sizes using oil mixtures (OEO + SO). The stabilization mechanism was not purely Pickering, as there was a reduction in interfacial tension. Excellent antimicrobial activity was observed against bacteria and the fungus Alternaria alternata, demonstrating the ability to apply these emulsions in active systems such as coatings and films. An improvement in the stability of emulsions was observed when using a mixture of oils, which is extremely advantageous considering costs and stability to heat treatments, since the desired antimicrobial activity is maintained for the final application.

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Phytopathogenic fungi Alternaria alternata and Colletotrichum gloeosporioides cause diseases in plant tissues as well as significant postharvest losses. The use of chemical fungicides for their control has negative effects on health and the environment. Secondary metabolites from halophilic bacteria are a promising alternative for new antifungal compounds. In the present study, halophilic bacteria were isolated and characterized from two sites with saline soils called branquizales in Campeche, Mexico. A total of 64 bacteria were isolated. Agrobacterium, Bacillus, Inquilinus, Gracilibacillus, Metabacillus, Neobacillus, Paenibacillus, Priestia, Staphylococcus, Streptomyces and Virgibacillus were among the identified genera. The antifungal potential of the culture supernatant (CS) of 39 halophilic bacteria was investigated against C. gloeosporioides and A. alternata. The bacteria showing the greatest inhibition of mycelial growth corresponded to Bacillus subtilis CPO 4292, Metabacillus sp. CPO 4266, Bacillus sp. CPO 4295 and Bacillus sp. CPO 4279. The CS of Bacillus sp. CPO 4279 exhibited the highest activity and its ethyl acetate extract (AcOEt) inhibited the germination of C. gloeosporioides, with IC50 values of 8,630µg/ml and IC90 of 10,720µg/ml. The organic partition of the AcOEt extract led to three fractions, with acetonitrile (FAcB9) showing the highest antifungal activity, with values exceeding 66%. Halophilic bacteria from 'blanquizales' soils of the genus Bacillus sp. produce metabolites with antifungal properties that inhibit the phytopathogenic fungus C. gloeosporioides.

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Fungal green biosynthesis of nanoparticles (NPs) is a promising eco-friendly method for mass-scale production. In the present study Ag, CuO and ZnO nanoparticles were biogenically synthetized using a cell filtrate of a strain of Trichoderma harzianum as a reducer and stabilizer agent. The structure, morphology and physicochemical properties of the NPs were characterized through transmission electron microscopy, dynamic light scattering, wide angle X-ray scattering and thermogravimetric analysis. Since nanotechnology could offer promising applications in agricultural area, we evaluated the ability of the NPs to reduce the growth of important fungal phytopathogens as Alternaria alternata, Pyricularia oryzae and Sclerotinia sclerotiorum. Silver and CuO NPs reduced significantly the mycelial growth of A. alternata and P. oryzae in a dose dependent manner. This is the first report of a multiple extracellular biosynthesis of NPs from T. harzianum and the first time that CuO and ZnO NPs were obtained from this fungus. In addition, we highlighted the rapid production of NPs, as well as, the potential of Ag and CuO for the control of phytopathogens. On the other hand, the three types of NPs could be easily and sustainably produced on a large scale with the chance of having multiple applications in biotechnological processes.

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Abstract Dark septate endophytes (DSE) are a heterogeneous group of fungi, mostly belonging to the Phylum Ascomycota, that are involved in a mutualistic symbiosis with plant roots. The aim of this study is to evaluate the behavior of two strains of DSE isolated from wheat roots of two cropping areas in the province of Buenos Aires, Argentina, against some agrochemicals. Of all the isolates obtained, two strains were identified as Alternaria alternata and Cochliobolus sp. These DSE were found to be tolerant to glyphosate, carbendazim and cypermethrin when evaluated at the recommended agronomic dose (AD), 2 AD and, in some cases, 10 AD. This work contributes to the study of the biology of this group of fungi and their tolerance in the presence of xenobiotics widely used in agriculture.© 2019 Asociaci´on Argentina de Microbiolog´ıa. Published by Elsevier Espa˜na, S.L.U. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/bync-nd/4.0/).

Resumen Los endófitos septados oscuros (DSE) son un grupo heterogéneo de hongos que participan de una simbiosis mutualista con raíces de plantas, perteneciendo principalmente al Phylum Ascomycota. El objetivo de este estudio fue aislar DSE de raíces de trigo proveniente de dos áreas de cultivo de la provincia de Buenos Aires y evaluar el comportamiento de dos cepas de DSE aisladas de raíces de trigo frente a algunos agroquímicos en dos áreas de cultivo de la provincia de Buenos Aires. De todos los aislamientos obtenidos se seleccionaron dos cepas que se identificaron como Alternaria alternata y Cochliobolus sp. Se encontró que estos DSE son tolerantes al glifosato, el carbendazim y la cipermetrina, evaluados a las dosis agronómicas recomendadas (AD), a 2x AD y, en algunos casos, a 10x AD. Este trabajo contribuye al conocimiento de la biología de este grupo de hongos y su tolerancia a xenobióticos ampliamente utilizados en la agricultura.

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Dark septate endophytes (DSE) are a heterogeneous group of fungi, mostly belonging to the Phylum Ascomycota, that are involved in a mutualistic symbiosis with plant roots. The aim of this study is to evaluate the behavior of two strains of DSE isolated from wheat roots of two cropping areas in the province of Buenos Aires, Argentina, against some agrochemicals. Of all the isolates obtained, two strains were identified as Alternaria alternata and Cochliobolus sp. These DSE were found to be tolerant to glyphosate, carbendazim and cypermethrin when evaluated at the recommended agronomic dose (AD), 2 AD and, in some cases, 10 AD. This work contributes to the study of the biology of this group of fungi and their tolerance in the presence of xenobiotics widely used in agriculture.

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Three experiments were carried out in an experimental field from Viçosa city, Minas Gerais, Brazil, to compare late blight (LB) (Phytophthora infestans) and early blight (EB) (Alternaria grandis) epidemics under different environmental conditions and fungicide application programs. Each experiment consisted of two side-by-side trials that were arranged in a randomized complete block design with five treatments and five replications. At 30 days after planting the potato (Ágata cultivar) plants in plots of trials 1 and 2 were inoculated with an isolate of P. infestans (A2 mating type) and four isolates of A. grandis, respectively. Fungicide applications were initiated at seven days after inoculation and repeated at 7- or 15-day intervals. The severity of LB and EB was assessed every two days, and the area under disease progress curve (AUDPC) was calculated for both diseases. The yield was quantified by weighing the tubers of all the plants from each plot after their maturation. Yield data were converted into kg ha-1 and the percentage of loss was calculated for each treatment. Based on the AUDPC, the LB severity was greater than the EB severity under all three environmental conditions, and this was reflected in the yield. LB and EB caused yield losses as high as of 82 and 45%, respectively. The fungicide effects on both LB and EB and the associated yield losses changed according to the environmental conditions, therefore, indicating the importance of using tools such as a forecast system to help farmers to decide the best time for fungicide application.

Três experimentos foram conduzidos em um campo experimental na cidade de Viçosa, localizada no estado de Minas Gerais, Brasil, para comparar epidemias de requeima (RE) (Phytophthora infestans) e pinta preta (PP) (Alternaria grandis) em diferentes condições ambientais e programas de aplicação de fungicidas. Cada experimento consistiu em dois ensaios lado a lado seguindo um delineamento de blocos casualizados com cinco tratamentos e cinco repetições. Aos 30 dias após o plantio dos tubérculos de batata do cultivar "Ágata", as plantas das parcelas dos ensaios 1 e 2 foram inoculadas com um isolado de P. infestans (grupo de compatibilidade A2) e quatro isolados de A. grandis, respectivamente. As aplicações de fungicida foram iniciadas aos sete dias após a inoculação e repetidas em intervalos de 7 ou 15 dias. A severidade da RE e PP foi avaliada a cada dois dias e a área abaixo da curva de progresso da doença (AACPD) foi calculada para ambas as doenças. A produtividade foi quantificada pesando os tubérculos de todas as plantas de cada parcela após a maturação. Produtividade foi convertida para kg ha-1 e a percentagem de redução da produtividade foi determinada para cada tratamento. Com base na AACPD, a severidade da RE foi maior que a severidade da PP nas três condições ambientais e refletiu na produtividade. A redução de produtividade foi de até 82 e 45% para RE e PP, respectivamente. A eficiência dos fungicidas no controle de RE e PP e as reduções na produtividade mudaram de acordo com o ambiente, indicando, portanto, a importância de usar ferramentas como um sistema de previsão para ajudar os agricultores a decidir o melhor momento para a aplicação de fungicidas.

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Three experiments were carried out in an experimental field from Viçosa city, Minas Gerais, Brazil, to compare late blight (LB) (Phytophthora infestans) and early blight (EB) (Alternaria grandis) epidemics under different environmental conditions and fungicide application programs. Each experiment consisted of two side-by-side trials that were arranged in a randomized complete block design with five treatments and five replications. At 30 days after planting the potato (Ágata cultivar) plants in plots of trials 1 and 2 were inoculated with an isolate of P. infestans (A2 mating type) and four isolates of A. grandis, respectively. Fungicide applications were initiated at seven days after inoculation and repeated at 7- or 15-day intervals. The severity of LB and EB was assessed every two days, and the area under disease progress curve (AUDPC) was calculated for both diseases. The yield was quantified by weighing the tubers of all the plants from each plot after their maturation. Yield data were converted into kg ha-1 and the percentage of loss was calculated for each treatment. Based on the AUDPC, the LB severity was greater than the EB severity under all three environmental conditions, and this was reflected in the yield. LB and EB caused yield losses as high as of 82 and 45%, respectively. The fungicide effects on both LB and EB and the associated yield losses changed according to the environmental conditions, therefore, indicating the importance of using tools such as a forecast system to help farmers to decide the best time for fungicide application.(AU)

Três experimentos foram conduzidos em um campo experimental na cidade de Viçosa, localizada no estado de Minas Gerais, Brasil, para comparar epidemias de requeima (RE) (Phytophthora infestans) e pinta preta (PP) (Alternaria grandis) em diferentes condições ambientais e programas de aplicação de fungicidas. Cada experimento consistiu em dois ensaios lado a lado seguindo um delineamento de blocos casualizados com cinco tratamentos e cinco repetições. Aos 30 dias após o plantio dos tubérculos de batata do cultivar "Ágata", as plantas das parcelas dos ensaios 1 e 2 foram inoculadas com um isolado de P. infestans (grupo de compatibilidade A2) e quatro isolados de A. grandis, respectivamente. As aplicações de fungicida foram iniciadas aos sete dias após a inoculação e repetidas em intervalos de 7 ou 15 dias. A severidade da RE e PP foi avaliada a cada dois dias e a área abaixo da curva de progresso da doença (AACPD) foi calculada para ambas as doenças. A produtividade foi quantificada pesando os tubérculos de todas as plantas de cada parcela após a maturação. Produtividade foi convertida para kg ha-1 e a percentagem de redução da produtividade foi determinada para cada tratamento. Com base na AACPD, a severidade da RE foi maior que a severidade da PP nas três condições ambientais e refletiu na produtividade. A redução de produtividade foi de até 82 e 45% para RE e PP, respectivamente. A eficiência dos fungicidas no controle de RE e PP e as reduções na produtividade mudaram de acordo com o ambiente, indicando, portanto, a importância de usar ferramentas como um sistema de previsão para ajudar os agricultores a decidir o melhor momento para a aplicação de fungicidas.(AU)

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The toxicity of four essential oils extracted from Baccharis articulata, Baccharis ochracea, Baccharis psiadioides and Baccharis trimera was tested against the phytopathogen Alternaria alternata, which causes Alternaria stem canker on tomatoes. Diseases caused by Alternaria fungi are responsible for great economic losses in terms of production and are controlled by synthetic fungicides; however, essential oils offer an alternative, since they have been proven to be effective for controlling against various plant pathogens. In this way, the antifungal activity of Baccharis essential oils was tested using potato dextrose agar medium with concentrations ranging from 0.1 to 20.0 µL mL-1. Baccharis trimera and Baccharis ochracea essential oils presented 100% mycelial growth inhibition of A. alternata and were also able to control Alternaria stem canker disease under greenhouse conditions. Tomato plants treated with these essential oils exhibited area under the disease progress curve (AUDPC) values of 230.10 and 241.42, differing from the control condition, which showed an AUDPC value of 268.92. The essential oils of B. trimera and B. ochracea can be an alternative for controlling Alternaria stem canker disease of tomatoes and should be formulated as a potential fungicide against the A. alternata pathogen.

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O objetivo deste trabalho foi determinar a atividade antimicrobiana do carvacrol e sua combinação com tiabendazol no controle de fungos patogênicos deteriorantes de frutas (Colletotrichum gloesporioides, Fusarium solani e Alternaria alternata). O carvacrol apresentou uma concentração inibitória mínima (CIM) de 282 a 563 μg mL-1 para os fungos testados. Quando avaliado em conjunto com o tiabendazol apresentou efeito aditivo contra C. gloesporioides e F. solani (FICI 0,5 e 1,0, respectivamente) e sinérgico contra a A.alternata (FICI 0,1). Houve redução da CIM do carvacrol de 50 a 88%. Este estudo mostra o potencial do uso.

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O objetivo deste trabalho foi determinar a atividade antimicrobiana do carvacrol e sua combinação com tiabendazol no controle de fungos patogênicos deteriorantes de frutas (Colletotrichum gloesporioides, Fusarium solani e Alternaria alternata). O carvacrol apresentou uma concentração inibitória mínima (CIM) de 282 a 563 μg mL-1 para os fungos testados. Quando avaliado em conjunto com o tiabendazol apresentou efeito aditivo contra C. gloesporioides e F. solani (FICI 0,5 e 1,0, respectivamente) e sinérgico contra a A.alternata (FICI 0,1). Houve redução da CIM do carvacrol de 50 a 88%. Este estudo mostra o potencial do uso.(AU)

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