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Although multiple outbreak clusters of Cyclospora cayetanensis have been traced back to consumption of dishes in Mexican-style restaurants, the FDA Bacteriological Analytical Manual (BAM) does not currently provide methods to detect C. cayetanensis in dishes that contain multiple produce ingredients, such as salsas and guacamole. These complex food matrices also may contain high levels of fats, which can interfere with the detection. Several modifications to the BAM Chapter 19b method (washing produce, DNA extraction, and a TaqMan real-time PCR assay targeting the 18S rRNA gene of C. cayetanensis) were assessed with the goal to detect as few as 5 oocysts of C. cayetanensis in 25 g samples of commercial salsa/pico de gallo, guacamole, and salsa verde. Both freshly prepared and frozen versions of these foods were seeded with 5, 10 and 200 oocysts. For salsa samples, using a gentler washing step than recommended by BAM, we achieved detection of 5 oocysts in the samples (81.8%, n = 11). Increasing the amount of Alconox® in the wash solution to 1%, rather than the 0.1% used in BAM, and adjusting the DNA extraction protocol to process large wash pellets, enabled detection of 5 oocysts in guacamole. To reach the desired level of detection in salsa verde, two types of modifications were necessary: gentler washing and DNA extraction modifications. The use of these same method modifications on previously frozen food samples, provided levels of detection similar to those achieved with fresh dishes. Our modifications enabled robust and reproducible detection of C. cayetanensis in multi-ingredient Mexican dishes, detecting as few as 5 oocysts in 25 g samples. Validating and deploying effective methods to detect C. cayetanensis in high risk fresh produce and prepared dishes are critically important for prevalence studies and outbreak investigations of this parasite.

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Knowledge about the net lactation energy (NLE) contained in the dry matter of grasses is necessary to make decisions about forage and the balance of diets for grazing cattle. Its determination is made in laboratories using wet or dry chemistry methods, which are costly, delayed, and sometimes present sampling- or process-related reliability problems. An algorithm, which analyzes the red-green-blue (RGB) images of grasses taken by drone, has been developed as a technological alternative. This has allowed us estimating the NLE level, reducing costs, and changing the sampling system and analysis method. The objective of the present study was to compare the milk production, which was calculated from the NLE and estimated using the algorithm for analysis of RGB images of grasses (included in the TaurusWebs® software), vs the actual milk production. The study was conducted in 15 dairy farms belonging to the dairy control system of the Colácteos dairy cooperative, which are located in the upper tropical region (Department of Nariño, Colombia). The prairies evaluated were composed of mixtures of Kikuyo (Pennisetum clandestinum), Raigrás (Lolium spp), and False Poa (Holcus lanatus). The result was analyzed using a linear regression model (R²=0.86; R=0.93). In the Student´s t-test, the actual and estimated milk production averages were equal (P>0.05). In conclusion, the NLE calculated using the algorithm satisfactorily explains the study livestock production, and the information generated by the algorithm can be used to calculate the NLE of grasses.(AU)

O conhecimento sobre a energia líquida de lactação (NLE) contida na matéria seca das gramíneas é necessário para a tomada de decisões sobre forragem e o equilíbrio das dietas para pastagem. Sua determinação é feita em laboratórios usando métodos de química úmida ou seca, que são dispendiosos, atrasados e às vezes apresentam problemas de confiabilidade relacionados a amostras ou processos. Um algoritmo, que avalia as imagens vermelho-verde-azul (RGB) de gramíneas tiradas por drone, foi desenvolvido como uma alternativa tecnológica. Isso nos permitiu estimar o nível de NLE, reduzir custos e alterar o sistema de amostragem e o método de análise. O objetivo do presente estudo foi comparar a produção de leite, calculada a partir do NLE e estimada usando o algoritmo para análise de imagens RGB de gramíneas (incluídas no software TaurusWebs®) versus a produção real de leite. O estudo foi realizado em 15 fazendas leiteiras pertencentes ao sistema de controle de laticínios da cooperativa de laticínios Colácteos, localizada na região tropical superior (Departamento de Nariño, Colômbia). As pradarias avaliadas foram compostas por misturas de Kikuyo (Pennisetum clandestinum), Raigrás (Lolium spp) e False Poa (Holcus lanatus). O resultado foi analisado usando um modelo de regressão linear (R² = 0,86; R = 0,93). No teste t de Student, as médias reais e estimadas de produção de leite foram iguais (P> 0,05). Em conclusão, o NLE calculado usando o algoritmo explica satisfatoriamente a produção animal estudada, e as informações geradas pelo algoritmo podem ser usadas para calcular o NLE das gramíneas.(AU)

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The objective of this research was to elaborate and characterize mortadella using fillet residues (V-cut fillet trim) of Nile tilapia, in order to add value to this by-product of the filleting process. Three mortadellas were made, one with 100% tilapia fillet trimmings, another containing 100% chicken meat and the third with 100% pork meat. Mortadellae were characterized in terms of microbiology, chemical composition, calcium, collagen, pH, Aw, colour, texture and formulation cost. Mortadella was within the recommended microbiological standards. Tilapia mortadella had higher levels of moisture, ash, calcium and collagen, higher pH and lower water activity when compared to other species. The tilapia mortadella had lower brightness, higher chroma a * and intermediate chroma b *, compared with the others. The texture of tilapia mortadella was better in terms of hardness, gumminess and chewability, the values of which were lower. The chicken mortadella had a higher acceptance rate; however, that of tilapia was also high, while all evaluated attributes of pork received the worst grades. Nile tilapia mortadella is a technological innovation that can be introduced into the food sector with good nutritional value and a good acceptance index.(AU)

O objetivo deste trabalho foi elaborar e caracterizar mortadelas a partir de aparas da filetagem (corte em V do filé) de tilápia do Nilo, de forma a possibilitar a agregação de valor a este subproduto do processo de filetagem. Foram elaboradas três mortadelas, sendo uma com 100% de aparas da filetagem de tilápia, outra contendo 100% de carne de frango e a terceira com 100% de carne suína. As mortadelas foram caracterizadas quanto à microbiologia, composição química, cálcio, colágeno, pH, Aw, cor, textura e custo de formulação. As mortadelas estavam dentro dos padrões microbiológicos recomendados. A mortadela de tilápia apresentou maiores teores de umidade, cinzas, cálcio e colágeno, maior pH e menor atividade de água quando comparada às demais espécies. A mortadela de tilápia apresentou menor luminosidade, maior croma a* e croma b* intermediário às demais. A textura foi melhor para as mortadelas de tilápia, quanto a dureza, gomosidade e mastigabilidade, cujos valores foram menores. A mortadela de frango teve maior índice de aceitação, porém, a de tilápia também foi elevado, enquanto de suíno todos os atributos avaliados receberam as piores notas. A mortadela de tilápia do Nilo é uma inovação tecnológica que pode ser introduzida no setor alimentício com bom valor nutricional e bom índice de aceitação.(AU)

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A versatile, rapid and safe green method for chlorine and sulfur determination using ion chromatography in cereals and legumes was developed. Microwave-induced combustion was evaluated for sample preparation. Ultrapure water and alkaline solutions were assessed for absorption of the analytes. Water was selected because good recoveries (97-109%) were obtained for both analytes. Low consumption of reagents and small quantities of waste are two important advantages of the proposed method. Accuracy was evaluated by analysis of a standard reference material, which agreed with certified values (91-101%). The results for repeatability (RSDs ≤ 4%) and intermediate precision (RSDs ≤ 7%) prove the good precision of the proposed method. Limits of quantification were 16 and 17 mg kg-1 for Cl and S, respectively. Concentrations of Cl and S varied across a wide range (Cl: 35-930 mg kg-1; S: 678-5124 mg kg-1) for 34 samples analyzed, which were, for most of the results, close to the values found in the literature.

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A methodology for the rapid determination of the aromatic compounds methyl anthranilate (MA), 2'-aminoacetophenone (2-AAP) and furaneol by GC-MS was validated and used to characterize grape juice and wine elaborated with the new Brazilian grape varieties cultivated in northeastern Brazil, and Brazilian grape nectars. The method presented linearity (R2 ˃ 0.9952), good accuracy (CV < 12.9%), recovery (76.6% to 106.3%), limit of detection (23 µg L-1 to 94 µg L-1) and limit of quantification (96 µg L-1 to 277 µg L-1) acceptable in only 20 min of running. The methodology was considered satisfactory for the purpose, being a simple and rapid method for the determination of these compounds in grape derivatives drinks. In the characterization of the nectars the compound that stood out was the MA, being its presence attributed to the addition of flavorings in these products. It was evidenced a significant contribution of furaneol in the aroma of grape juice and wines elaborated with the new Brazilian grape varieties.

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In this study, a QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method, optimized by a 23 full factorial design, was developed for the determination of 72 pesticides in plant parts of carrot, corn, melon, rice, soy, silage, tobacco, cassava, lettuce and wheat by ultra-high-performance liquid chromatographic tandem mass spectrometry (UHPLC-MS/MS). Considering the complexity of these matrices and the need of use calibration in matrix, a new calibration approach based on single level standard addition in the sample (SLSAS) was proposed in this work and compared with the matrix-matched calibration (MMC), the procedural standard calibration (PSC) and the diluted standard addition calibration (DSAC). All approaches presented satisfactory validation parameters with recoveries from 70 to 120% and relative standard deviations≤20%. SLSAS was the most practical from the evaluated approaches and proved to be an effective way of calibration. Method limit of detection were between 4.8 and 48µgkg-1 and limit of quantification were from 16 to 160µgkg-1. Method application to different kinds of plants found residues of 20 pesticides that were quantified with z-scores values≤2 in comparison with other calibration approaches. The proposed QuEChERS method combined with UHPLC-MS/MS analysis and using an easy and effective calibration procedure presented satisfactory results for pesticide residues determination in different crop plants and is a good alternative for routine analysis.

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This study evaluated the nutritional characteristics of hay of nineteen hybrids between sorghum and Sudan grass. The experimental design was a randomized block with nineteen treatments and three replications. The chemical characteristics of the respective hays were analyzed 52 days after sowing and 45 and 49 regrowth days. Data were analyzed by analysis of variance, and when the F test was significant, the treatment means were compared by the Scott and Knott test at 5%. Genotypes 1013026 and 1014020 showed similar dry matter content. As for ether extract content, the highest values ranged from 2.20 to 2.66%. Higher values of minerals were observed in hybrids 1013026 and 1011005. In relation to crude protein, higher content values were reported for hybrids 1013021 and 1013020 (14.59 and 15.47% CP). When evaluating the neutral detergent fiber content, lower values varied between 56.05 and 57.07% for hybrids 1013016 and 1011009. Considering the content of acid detergent fiber, values ranged from 32.70 to 35.78%. Values of dry matter digestibility were higher than 50%. All hybrids provide quality hay, however the hybrid 1014019 showed the best nutritional value.(AU)

Este trabalho foi realizado para avaliar as características nutricionais de feno de 19 genótipos de sorgo com capim-sudão. O delineamento experimental utilizado foi em blocos casualizados composto por 19 tratamentos com três repetições. As características bromatológicas dos respectivos fenos foram analisadas após 52 dias de semeadura e 45 e 49 dias de rebrota. Os dados foram interpretados pela análise de variância e, quando o teste de F foi significativo, as médias dos tratamentos foram comparadas pelo teste de Scott e Knott em nível de 5%. Os genótipos 1013026 e 1014020 apresentaram teores semelhantes de matéria seca. Para a variável extrato etéreo os maiores valores oscilaram de 2,20 a 2,66%. Valores superiores de minerais foram evidenciados nos híbridos 1013026 e 1011005. Quanto à proteína bruta, os maiores teores foram reportados pelos híbridos 1013021 e 1013020 (14,59 e 15,47 % de PB). Ao avaliar os valores de fibra em detergente neutro, os menores teores variaram de 56,05 a 57,07% para os híbridos 1013016 e 1011009. A fibra em detergente ácido variou de 32,70 a 35,78%. Os valores de digestibilidade da matéria seca foram superiores a 50%. Todos os híbridos apresentaram feno de qualidade, no entanto o híbrido 1014019 apresentou melhor valor nutritivo.(AU)

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This study evaluated the nutritional characteristics of hay of nineteen hybrids between sorghum and Sudan grass. The experimental design was a randomized block with nineteen treatments and three replications. The chemical characteristics of the respective hays were analyzed 52 days after sowing and 45 and 49 regrowth days. Data were analyzed by analysis of variance, and when the F test was significant, the treatment means were compared by the Scott and Knott test at 5%. Genotypes 1013026 and 1014020 showed similar dry matter content. As for ether extract content, the highest values ranged from 2.20 to 2.66%. Higher values of minerals were observed in hybrids 1013026 and 1011005. In relation to crude protein, higher content values were reported for hybrids 1013021 and 1013020 (14.59 and 15.47% CP). When evaluating the neutral detergent fiber content, lower values varied between 56.05 and 57.07% for hybrids 1013016 and 1011009. Considering the content of acid detergent fiber, values ranged from 32.70 to 35.78%. Values of dry matter digestibility were higher than 50%. All hybrids provide quality hay, however the hybrid 1014019 showed the best nutritional value.

Este trabalho foi realizado para avaliar as características nutricionais de feno de 19 genótipos de sorgo com capim-sudão. O delineamento experimental utilizado foi em blocos casualizados composto por 19 tratamentos com três repetições. As características bromatológicas dos respectivos fenos foram analisadas após 52 dias de semeadura e 45 e 49 dias de rebrota. Os dados foram interpretados pela análise de variância e, quando o teste de F foi significativo, as médias dos tratamentos foram comparadas pelo teste de Scott e Knott em nível de 5%. Os genótipos 1013026 e 1014020 apresentaram teores semelhantes de matéria seca. Para a variável extrato etéreo os maiores valores oscilaram de 2,20 a 2,66%. Valores superiores de minerais foram evidenciados nos híbridos 1013026 e 1011005. Quanto à proteína bruta, os maiores teores foram reportados pelos híbridos 1013021 e 1013020 (14,59 e 15,47 % de PB). Ao avaliar os valores de fibra em detergente neutro, os menores teores variaram de 56,05 a 57,07% para os híbridos 1013016 e 1011009. A fibra em detergente ácido variou de 32,70 a 35,78%. Os valores de digestibilidade da matéria seca foram superiores a 50%. Todos os híbridos apresentaram feno de qualidade, no entanto o híbrido 1014019 apresentou melhor valor nutritivo.

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O objetivo deste estudo foi avaliar o tempo e a temperatura das refeições transportadas na modalidade self service em uma Unidade de Alimentação e Nutrição em São Luís/MA. Foram aferidas as temperaturas de três tipos de refeições quentes: arroz, feijão e guarnição (macarrão) verificando-se o horário em que elas foram expostas e colocadas nas cubas inox para serem transportadas até o cliente final em diferentes etapas: quando o alimento foi colocado nas cubas antes de serem transportados e após os clientes servirem-se. O arroz e o feijão apresentaram temperaturas iniciais e finais adequadas como preconiza a RDC 216 (ANVISA). A guarnição iniciou com temperatura abaixo do recomendado (57,83°C), porém, após ser colocada no balcão de distribuição, atingiu temperatura final adequada (64°C). Conclui-se que o tempo e a temperatura dos alimentos na UAN pesquisada, conservaram-se dentro dos padrões, uma medida importante para que os micro-organismos patogênicos e deteriorantes não sofram proliferação nos alimentos expostos.(AU)

This study aimed to evaluate the time and temperature of meals transported in self-service mode on a Food and Nutrition Unit in San Luis-MA. Temperatures were measured three kinds of hot meals: rice, beans and trim. (noodles) verifying the time that they were exposed and placed in stainless steel tanks to be transported to the end customer in different stages: when food was placed in vats before being transported; when he was at the counter and heat after customers finished their meals. The rice and beans had adequate temperatures as recommended by the RDC 216 (ANVISA). The garrison began with temperatures lower than recommended, but at the end of meals presented in accordance temperature. It is concluded that the time and temperature of food in UAN studied, conserved within the standard, an important measure for the proliferation does not undergo pathogens in foods exposed.(AU)

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This study aimed to evaluate the effects of inclusion of crambe bran concentrations (Crambe abyssinica Hochst.) with different specific masses in elephant grass silage (Penninsetum purpureum Schum.). For that, the bromatological and microbiological compositions of the experimental silages were determined. We used 48 mini silos distributed in a completely randomized design, arranged in a factorial 4 × 3, four levels of inclusion of crambe bran (0; 10; 20 and 30%) and three specific masses (400; 500 and 600 kg MN m-3), with four replications. After 240 days of fermentation the silos were opened. It was observed linear effect on DM, CP, NDFap, ADFap, HEM, LIG, NFC, TC and population of bacteria that produce lactic acid with the addition of crambe meal. There was interaction between the crambe bran factors and specific masses on the values of pH and N-NH3. For MM variables and yeast count there was a negative linear effect due to the evaluated specific mass. The inclusion of crambe bran helps to increase the fermentative profile and the bromatological composition of elephant grass silages studied, and the best results were obtained with the addition of 30% of this coproduct, based on the natural matter.

Objetivou-se avaliar os efeitos da inclusão de concentrações do farelo de crambe (Crambe abyssinica Hochst.), com diferentes massas específicas, na ensilagem de capim-elefante (Penninsetum purpureum Schum.). Para tanto foram determinadas as composições bromatológica e microbiológica das silagens experimentais. Foram utilizados 48 minissilos distribuídos em delineamento inteiramente casualizado, arranjado em esquema fatorial 4 × 3, sendo quatro concentrações de inclusão do farelo de crambe (0; 10; 20 e 30%) e três massas específicas (400; 500 e 600 kg de MN m-3), com quatro repetições. Após 240 dias de fermentação os silos foram abertos. Foi observado efeito linear para os teores de MS, PB, FDNcp, FDAcp, HEM, LIG, CNF, CT e para a população de bactérias produtoras de ácido lático com a inclusão do farelo de crambe. Houve interação entre os fatores farelo de crambe e massas específicas sobre os valores de pH e N-NH3. Para as variáveis MM e contagem de leveduras houve efeito linear negativo em função da massa específica avaliada. A inclusão do farelo de crambe contribui para incrementar o perfil fermentativo e a composição bromatológica das silagens de capim-elefante estudadas, sendo os melhores resultados obtidos com a adição de 30% deste coproduto, com base na matéria natural.

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