RESUMO
The Richeria grandis known is for its aphrodisiac properties and due to its wide empiric use well as thelack of gender Richeria studies the objective of this study was to evaluate the androgenic and anti-androgenicactivity of this plant. They were used in this study 64 male Wistar rats, castrated, divided into eight groups. Thegroups for the study of androgen activity were treated with boiling water over corn R. grandis ethanolic extractoil at doses of 75, 150 and 300 mg / kg and groups of antiandrogenic activity received corn oil, distilled waterand ethanol extract at doses of 75, 150 and 300 mg / kg associated with testosterone. It was found that theethanol extract of R. grandis in the tested doses did not differ statistically from the negative and positive controlsdid not thus demonstrating androgenic activity or antiandrogenic.
Assuntos
Animais , Ratos , Androgênios/administração & dosagem , Androgênios/análise , Antagonistas de Androgênios/análise , Malpighiales/anatomia & histologia , Malpighiales/fisiologia , Malpighiales/químicaRESUMO
Although the high expression of the northeastern herds, the levels of productivity, reproducibility,carcass gain and other factors are low. This is mainly due to the main food source being from native plants ofthe Savanna. Were used male Wistar rats (64) divided into eight groups. These animals were castrated and, afterrecovery, received ethanol extract of pods of Senna spectabilis (EEVSs) in three different doses and testosteroneintramuscularly (some groups). The animals were treated for 32 consecutive days and at the end wereeuthanized. The organs were collected and weighed for evaluation of androgenic and anti-androgenic activity.The extract showed no androgenic nor anti-androgenic activity at the doses tested.
Assuntos
Animais , Ratos , Androgênios/administração & dosagem , Androgênios/análise , Antagonistas de Androgênios/administração & dosagem , Antagonistas de Androgênios/análise , Extrato de Senna/análise , Extrato de Senna/química , RuminantesRESUMO
Although the high expression of the northeastern herds, the levels of productivity, reproducibility,carcass gain and other factors are low. This is mainly due to the main food source being from native plants ofthe Savanna. Were used male Wistar rats (64) divided into eight groups. These animals were castrated and, afterrecovery, received ethanol extract of pods of Senna spectabilis (EEVSs) in three different doses and testosteroneintramuscularly (some groups). The animals were treated for 32 consecutive days and at the end wereeuthanized. The organs were collected and weighed for evaluation of androgenic and anti-androgenic activity.The extract showed no androgenic nor anti-androgenic activity at the doses tested.(AU)
Assuntos
Animais , Ratos , Androgênios/administração & dosagem , Androgênios/análise , Antagonistas de Androgênios/administração & dosagem , Antagonistas de Androgênios/análise , Extrato de Senna/análise , Extrato de Senna/química , RuminantesRESUMO
The Richeria grandis known is for its aphrodisiac properties and due to its wide empiric use well as thelack of gender Richeria studies the objective of this study was to evaluate the androgenic and anti-androgenicactivity of this plant. They were used in this study 64 male Wistar rats, castrated, divided into eight groups. Thegroups for the study of androgen activity were treated with boiling water over corn R. grandis ethanolic extractoil at doses of 75, 150 and 300 mg / kg and groups of antiandrogenic activity received corn oil, distilled waterand ethanol extract at doses of 75, 150 and 300 mg / kg associated with testosterone. It was found that theethanol extract of R. grandis in the tested doses did not differ statistically from the negative and positive controlsdid not thus demonstrating androgenic activity or antiandrogenic.(AU)
Assuntos
Animais , Ratos , Malpighiales/anatomia & histologia , Malpighiales/química , Malpighiales/fisiologia , Androgênios/administração & dosagem , Androgênios/análise , Antagonistas de Androgênios/análiseRESUMO
Cannabinoids can inhibit the release of androgens locally or centrally. For this reason, this study was designed to assess the effect of WIN 55, 212-2, a CB1 receptor agonist, on gubernacular development histologically. Sprague-Dawley female rats were time-mated and divided into treatment and control groups. For prenatal exposures, the groups received injections subcutaneously of 0.5 mg/kg WIN 55, 212-2 (WIN) or vehicle on gestational days 13.520.5. Five to 13 male offspring were collected at time points E19 (embryonic), P0 (postnatal), P2, and P8. The body weight and anogenital distance (AGD) of postnatal male pups were recorded at birth. The inguino-perineal region of all the samples after fixing in 4% paraformaldehyde were sectioned oblique-sagittally and stained with Hematoxilin and Eosin (HE) or Masson's trichrome. Measured Factors in this study were based on previous studies and included gubernacular cone height (GCH), gubernacular cone width (GCW), distance from gubernaculum to scrotum (G-S), and pubic symphysis-to-anus distance (PS-A). The former two factors were measured bilaterally and the latter two only on the left side. The gubernaculum at P0 appeared more bulky than that of controls. Failure of eversion at P2 and remaining bulb of gubernaculum at P8 were important findings in WIN-treated group. The mean distance from gubernaculum to scrotum increased significantly only atP2 compared to controls. AGD as a bioassay of fetal androgen action also showed a significant 16% reduction as compared with the control group at birth. These data propose that prenatal exposure to WIN can affect gubernacular development probably due to androgen-disruptive action.
Assuntos
Masculino , Animais , Ratos , Antagonistas de Androgênios/análise , Cordão Espermático/crescimento & desenvolvimento , Endocanabinoides/antagonistas & inibidores , Períneo/anatomia & histologia , Animais Recém-Nascidos/genéticaRESUMO
Cannabinoids can inhibit the release of androgens locally or centrally. For this reason, this study was designed to assess the effect of WIN 55, 212-2, a CB1 receptor agonist, on gubernacular development histologically. Sprague-Dawley female rats were time-mated and divided into treatment and control groups. For prenatal exposures, the groups received injections subcutaneously of 0.5 mg/kg WIN 55, 212-2 (WIN) or vehicle on gestational days 13.520.5. Five to 13 male offspring were collected at time points E19 (embryonic), P0 (postnatal), P2, and P8. The body weight and anogenital distance (AGD) of postnatal male pups were recorded at birth. The inguino-perineal region of all the samples after fixing in 4% paraformaldehyde were sectioned oblique-sagittally and stained with Hematoxilin and Eosin (HE) or Masson's trichrome. Measured Factors in this study were based on previous studies and included gubernacular cone height (GCH), gubernacular cone width (GCW), distance from gubernaculum to scrotum (G-S), and pubic symphysis-to-anus distance (PS-A). The former two factors were measured bilaterally and the latter two only on the left side. The gubernaculum at P0 appeared more bulky than that of controls. Failure of eversion at P2 and remaining bulb of gubernaculum at P8 were important findings in WIN-treated group. The mean distance from gubernaculum to scrotum increased significantly only atP2 compared to controls. AGD as a bioassay of fetal androgen action also showed a significant 16% reduction as compared with the control group at birth. These data propose that prenatal exposure to WIN can affect gubernacular development probably due to androgen-disruptive action.(AU)