RESUMO
Alphabaculovirus is an entomopathogenic virus genus that infects Bombyx mori, which is known as the Bombyx mori multiple nucleopolyedrovirus (BmMNPV). This virus is polyorganotrophic, and a series of tissues are known as targets; however, there is currently no information regarding infection in the pylorus, the segment of the hindgut that is present in the midgut transition and is responsible for food passage control. Thus, in the present study, we aimed to analyze infection of the B. mori pylorus by BmMNPV. To do so, hybrid B. mori larvae were inoculated with a viral suspension of BmMNPV, and segments of the intestine containing the pylorus and its subdivisions, the posterior interstitial ring (PIR), pyloric cone, and pyloric valve, were dissected and processed for light microscopy on different days post inoculation. The results showed that B. mori pylorus subdivisions respond differently to infection, and the anterior area of the PIR is susceptible with these cells being the secondary infection targets. Cytological analysis revealed the presence of viroplasm in the hypertrophic nucleus, followed by the formation and development of viral polyhedra. Cytolysis occurred at the end of the infectious cycle, thereby releasing polyhedra and enabling the spread of the disease. There was no evidence of BmMNPV infection in the posterior area of the PIR, cone, or pyloric valve. These results will contribute to greater understanding of the virus infectious cycle, whose consequent epizootic disease can negatively impact this economically important insect that is used in silk production in Brazil.
Assuntos
Bombyx/virologia , Trato Gastrointestinal/virologia , Larva/virologia , Nucleopoliedrovírus/crescimento & desenvolvimento , Animais , Bombyx/ultraestrutura , Brasil , Trato Gastrointestinal/ultraestrutura , Larva/ultraestrutura , Nucleopoliedrovírus/patogenicidade , Especificidade de Órgãos , Seda/biossínteseRESUMO
A qualidade da amoreira, medida pela umidade no ramo, folha e caule, e pela composição de nutrientes na folha, foi avaliada em material fresco e após armazenagem dos ramos em cinco sistemas (depósito de ramos 24 horas, sistema de cobertura úmida e imersão em água C/I 24 horas, C/I 48 horas, C/I 72 horas e C/I 96 horas). O delineamento experimental adotado foi o inteiramentecasualizado com seis tratamentos e seis repetições. A umidade e a composição de nutrientes na folha de amoreira foram mantidas em teores apropriados para alimentação do bicho-da-seda, tanto nos ramos armazenados em depósito quanto em sistema de cobertura e imersão por até 72 horas.(AU)
The quality of the mulberry, measured by moisture in the branch, leaf and stem and by leaf nutrient composition, wasevaluated in fresh material and after storage of the branches in five systems (branches depository - 24 hours, system of wet cover and immersion in water-C/I - 24 hours, C/I 48 hours,C/I 72 hours and C/I 96 hours). It was used a completely randomized design, with six treatments and six replications. The moisture and nutrient composition in the mulberry leaf weremaintained at levels appropriate for feeding of silkworm, as in the branches stored in depository as in systems of cover and immersion for up 72 hours.(AU)
Assuntos
Animais , Morus/citologia , Bombyx , Bombyx/crescimento & desenvolvimento , Bombyx/ultraestrutura , Umidade , Imersão/fisiopatologia , Ciências da Nutrição AnimalRESUMO
A qualidade da amoreira, medida pela umidade no ramo, folha e caule, e pela composição de nutrientes na folha, foi avaliada em material fresco e após armazenagem dos ramos em cinco sistemas (depósito de ramos 24 horas, sistema de cobertura úmida e imersão em água C/I 24 horas, C/I 48 horas, C/I 72 horas e C/I 96 horas). O delineamento experimental adotado foi o inteiramentecasualizado com seis tratamentos e seis repetições. A umidade e a composição de nutrientes na folha de amoreira foram mantidas em teores apropriados para alimentação do bicho-da-seda, tanto nos ramos armazenados em depósito quanto em sistema de cobertura e imersão por até 72 horas.
The quality of the mulberry, measured by moisture in the branch, leaf and stem and by leaf nutrient composition, wasevaluated in fresh material and after storage of the branches in five systems (branches depository - 24 hours, system of wet cover and immersion in water-C/I - 24 hours, C/I 48 hours,C/I 72 hours and C/I 96 hours). It was used a completely randomized design, with six treatments and six replications. The moisture and nutrient composition in the mulberry leaf weremaintained at levels appropriate for feeding of silkworm, as in the branches stored in depository as in systems of cover and immersion for up 72 hours.