RESUMO
Embryos of marine organisms whose development occurs externally are particularly sensitive to ultraviolet (UV) light (bands A and B, respectively, UVA and UVB). ATP-binding cassette (ABC) transporters are the first line of cellular defense against chemical or physical stress. The present work investigated the involvement of ABC transporters on UVA or UVB effects on eggs, spermatozoa, and embryonic cells of the sea urchin Echinometra lucunter. Gametes or embryos were exposed to UVA (3.6-14.4 kJ m(-2)) or UVB (0.112-14.4 kJ m(-2)), and embryonic development was monitored by optical microscopy at different developmental stages in the presence or absence of the ABC-transporter blockers reversin205 (ABCB1 blocker) or MK571 (ABCC1 blocker). E. lucunter eggs, spermatozoa and embryos were resistant to UVA exposure. Resistance to the harmful effects of UVB was strongly associated to ABC transporter activity (embryos > eggs > spermatozoa). ABCB1 or ABCC1 blockage promoted the injurious effects of UVA on spermatozoa. ABCC1 transporter blockage increased UVB-dependent damage in eggs while ABCB1 transporter inhibition increased harmful effects of UVB in embryonic cells. ABC-transporter activity was not, however, affected by UVB exposure. In conclusion, the present study is the first report on the protective role of ABC transporters against harmful effects of UVA and UVB on sea urchin eggs and embryonic cells.
Assuntos
Transportadores de Cassetes de Ligação de ATP/metabolismo , Desenvolvimento Embrionário/efeitos da radiação , Células-Tronco Embrionárias/efeitos da radiação , Células Germinativas/efeitos da radiação , Ouriços-do-Mar/embriologia , Raios Ultravioleta/efeitos adversos , Transportadores de Cassetes de Ligação de ATP/antagonistas & inibidores , Análise de Variância , Animais , Desenvolvimento Embrionário/efeitos dos fármacos , Células-Tronco Embrionárias/metabolismo , Células Germinativas/metabolismo , Microscopia , Oligopeptídeos/farmacologia , Propionatos/farmacologia , Quinolinas/farmacologia , Ouriços-do-Mar/citologiaRESUMO
The mechanisms underlying pluripotency and differentiation in embryonic and reprogrammed stem cells are unclear. In this work, we characterized the pluripotent state towards neural differentiated state through analysis of trace elements distribution using the Synchrotron Radiation X-ray Fluorescence Spectroscopy. Naive and neural-stimulated embryoid bodies (EB) derived from embryonic and induced pluripotent stem (ES and iPS) cells were irradiated with a spatial resolution of 20 µm to make elemental maps and qualitative chemical analyses. Results show that these embryo-like aggregates exhibit self-organization at the atomic level. Metallic elements content rises and consistent elemental polarization pattern of P and S in both mouse and human pluripotent stem cells were observed, indicating that neural differentiation and elemental polarization are strongly correlated.