Assuntos
Antioxidantes/metabolismo , Cádmio/toxicidade , Cálcio/metabolismo , Catalase/metabolismo , Superóxido Dismutase/metabolismo , Testículo/efeitos dos fármacos , Animais , Cádmio/farmacocinética , Calcinose/induzido quimicamente , Relação Dose-Resposta a Droga , Células Germinativas/efeitos dos fármacos , Células Germinativas/ultraestrutura , Masculino , Oxirredução , Ratos Wistar , Doenças Testiculares/induzido quimicamente , Testículo/enzimologia , Testículo/patologiaRESUMO
We present two cases of patients with systemic autoimmune diseases (one with dermatomyositis and one with CREST syndrome) who presented with a worsening of calcinosis cutis after treatment of osteoporosis with teriparatide. To our knowledge, this association is not described in the literature and might be considered in the spectrum of adverse reactions to teriparatide.
Assuntos
Conservadores da Densidade Óssea/efeitos adversos , Calcinose/induzido quimicamente , Osteoporose/tratamento farmacológico , Dermatopatias/induzido quimicamente , Teriparatida/efeitos adversos , Idoso , Síndrome CREST/complicações , Dermatomiosite/complicações , Feminino , Humanos , Pessoa de Meia-Idade , Osteoporose/complicaçõesRESUMO
INTRODUCTION: Nanomaterials have been used to create new consumer products as well as applications for life sciences and biotechnology. The aim of this study was to evaluate the tissue response to implanted polyethylene tubes filled with fibrin sponge embedded with silver nanoparticles dispersion. METHODS: Thirty rats received individually 4 polyethylene tubes filled with sponge embedded in 47 ppm, 23 ppm silver nanoparticles dispersion, 2.5% sodium hypochlorite, or with no embedding as control. The observation periods were 7, 15, 30, 60, and 90 days. After each period of time, 6 animals were killed, and the tubes and surrounding tissue were removed, fixed, and prepared to be analyzed in light microscope with glycol methacrylate embedding, 3-µm serial cutting, and hematoxylin-eosin stain. Qualitative and quantitative evaluations of the reactions were performed. RESULTS: Both materials caused moderate reactions at 7 days. The response was similar to the control on the 15th day with 23 ppm silver nanoparticles dispersion and 2.5% sodium hypochlorite and on the 30th day with 47 ppm silver nanoparticles dispersion. CONCLUSIONS: It was possible to conclude that silver nanoparticles dispersion was biocompatible especially in a lower concentration.
Assuntos
Tecido Conjuntivo/efeitos dos fármacos , Nanopartículas/toxicidade , Irrigantes do Canal Radicular/toxicidade , Prata/toxicidade , Animais , Calcinose/induzido quimicamente , Portadores de Fármacos , Fibrina , Masculino , Necrose/induzido quimicamente , Infiltração de Neutrófilos , Ratos , Ratos Wistar , Hipoclorito de Sódio/toxicidadeRESUMO
We report the case of a 50-year-old male patient with a rare profile: bronchial casts associated with the use of pegylated interferon and ribavirin. The patient sought treatment in a pulmonology clinic with a history of progressive dyspnea for four months that had evolved to progressive cough followed by frequent and abundant elimination of bronchial casts. The patient was initially treated with bronchodilators, as well as with oral and inhaled corticosteroids. Fiberoptic bronchoscopy, bronchoalveolar lavage and sputum analysis were carried out but did not contribute to the elucidation of the diagnosis. The symptoms developed while the patient was receiving pegylated interferon and ribavirin for the treatment of hepatitis C. The symptoms resolved 30 days after the discontinuation of the treatment. To our knowledge, this is the first report of bronchial casts caused by the use of pegylated interferon and ribavirin.
Assuntos
Antivirais/efeitos adversos , Broncopatias/induzido quimicamente , Calcinose/induzido quimicamente , Interferons/efeitos adversos , Ribavirina/efeitos adversos , Dispneia/tratamento farmacológico , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
Relatamos o caso de um homem de 50 anos com uma rara associação entre moldes brônquicos e o uso de interferon peguilado e ribavirina. O paciente procurou o serviço de pneumologia por apresentar, há quatro meses, dispneia progressiva que evoluiu com tosse progressiva seguida da expulsão de moldes brônquicos frequentes e abundantes. O paciente foi tratado inicialmente com medicações broncodilatadoras e uso de corticoide oral e inalatório. Foram realizados fibrobroncoscopia, lavado broncoalveolar e análise do escarro, os quais não acrescentaram informações para elucidação diagnóstica. Durante o período sintomático, o paciente fez uso de interferon peguilado e ribavirina para o tratamento de hepatite C. Após 30 dias da interrupção das medicações, o paciente ficou assintomático. Este é o primeiro relato sobre a associação entre molde brônquico e o uso de interferon peguilado e ribavirina.
We report the case of a 50-year-old male patient with a rare profile: bronchial casts associated with the use of pegylated interferon and ribavirin. The patient sought treatment in a pulmonology clinic with a history of progressive dyspnea for four months that had evolved to progressive cough followed by frequent and abundant elimination of bronchial casts. The patient was initially treated with bronchodilators, as well as with oral and inhaled corticosteroids. Fiberoptic bronchoscopy, bronchoalveolar lavage and sputum analysis were carried out but did not contribute to the elucidation of the diagnosis. The symptoms developed while the patient was receiving pegylated interferon and ribavirin for the treatment of hepatitis C. The symptoms resolved 30 days after the discontinuation of the treatment. To our knowledge, this is the first report of bronchial casts caused by the use of pegylated interferon and ribavirin.
Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Antivirais/efeitos adversos , Broncopatias/induzido quimicamente , Calcinose/induzido quimicamente , Interferons/efeitos adversos , Ribavirina/efeitos adversos , Dispneia/tratamento farmacológicoRESUMO
Calcification of glutaraldehyde-treated bioprosthetic heart valves is a major cause of long-term failure. We studied porcine aortic valves treated by the L-Hydro process and implanted into 14 juvenile sheep (group 1). Another 10 sheep were implanted with glutaraldehyde-treated porcine bioprostheses (group 2). The animals were sacrificed after 150 days and the explanted valves were analyzed for calcification. Hemodynamic measurements by echocardiography and angiography were carried out prior to sacrifice. Macroscopic analysis showed calcification and loss of mobility of the leaflets in all group 2 implants and in one group 1 implant. Light microscopy showed foci of calcification in all group 2 implants and in 3 valves from group 1. A significant reduction in the level of calcification was found in porcine bioprostheses treated by the L-Hydro process and implanted into the juvenile sheep model.
Assuntos
Valva Aórtica/cirurgia , Bioprótese , Calcinose/induzido quimicamente , Fixadores/efeitos adversos , Glutaral/efeitos adversos , Doenças das Valvas Cardíacas/induzido quimicamente , Implante de Prótese de Valva Cardíaca/instrumentação , Próteses Valvulares Cardíacas , Fixação de Tecidos/métodos , Animais , Valva Aórtica/patologia , Valva Aórtica/fisiopatologia , Calcinose/patologia , Calcinose/fisiopatologia , Análise de Falha de Equipamento , Feminino , Doenças das Valvas Cardíacas/patologia , Doenças das Valvas Cardíacas/fisiopatologia , Hemodinâmica , Masculino , Modelos Animais , Desenho de Prótese , Falha de Prótese , Ovinos , Resultado do TratamentoRESUMO
In vascular smooth muscle, calcium overload is a highly pathogenic event, which increases with advancing age. An increase in the calcium content of arterial wall may be produced in rats by treatment with vitamin D3. The aim of this study was to evaluate the renal clearance of sulfanilamide (a model organic anion, preferentially eliminated by the kidneys) and other parameters of global renal function in rats with arterial calcinosis. Arterial calcinosis was produced in adult rats by means of a single dose of vitamin D3 (300,000 UI/kg bw, i.m.) 5 days before the experiment. Treated rats showed a large increase in calcium content of aortic tissue and an increase in systolic arterial pressure. No modifications were observed in plasma calcium levels and in plasma lipid profiles. Statistically significant decrements were observed in renal clearance of sulfanilamide, in renal blood flow, in fractional excretion of sodium and potassium. A slight decrease, not statistically different, was observed in the glomerular filtration rate. Rats with arterial calcinosis also showed an increment of total calcium levels in renal tissue, in fractional excretion of calcium and in the expression of organic anion transporter 1 (OAT1). Histological studies revealed tubular alterations. In summary, modifications in hemodynamics and tubular parameters are early manifestations of nephropathy in rats with arterial calcinosis, some of which may account for the changes observed in organic anions renal depuration. It is important to mention that the decrease in clearance of organic anions were seen in spite of the increase in expression of OAT1.
Assuntos
Doenças da Aorta/metabolismo , Calcinose/metabolismo , Nefropatias/metabolismo , Animais , Aorta Abdominal/metabolismo , Doenças da Aorta/induzido quimicamente , Pressão Sanguínea/efeitos dos fármacos , Pressão Sanguínea/fisiologia , Calcinose/induzido quimicamente , Cálcio/metabolismo , Colecalciferol/efeitos adversos , Modelos Animais de Doenças , Taxa de Filtração Glomerular/efeitos dos fármacos , Taxa de Filtração Glomerular/fisiologia , Nefropatias/fisiopatologia , Testes de Função Renal , Masculino , Proteína 1 Transportadora de Ânions Orgânicos/biossíntese , Ensaios Clínicos Controlados Aleatórios como Assunto , Ratos , Ratos Wistar , Circulação Renal/efeitos dos fármacos , Circulação Renal/fisiologia , Sulfanilamida , Sulfanilamidas/metabolismo , Sulfanilamidas/farmacologia , Sístole/efeitos dos fármacos , Sístole/fisiologia , Fatores de TempoRESUMO
1. Ageing induces calcium accumulation in the vascular system. The simplest experimental way of producing high degrees of arterial calcium overload is by administration of an overdose of vitamin D(3) to rats. The aim of the present study was to evaluate the pharmacokinetics of organic anions in rats with arterial calcinosis induced by an overdose of vitamin D(3). 2. We used bromosulfophthalein (BSP) and sulfanilamide (SA) as models of organic anions with preferential biliary and renal excretion, respectively. 3. Increases in the clearance and elimination rate constant of BSP were observed in treated rats. The clearance and the elimination rate constant for SA were also increased in rats with arterial calcinosis. 4. Variations in arterial hepatic blood flow, aspartate aminotransferase activity and liver calcium accumulation were not observed in treated rats. In contrast, treated rats had a lower renal blood flow and increased renal calcium levels. 5. In summary, rats with arterial calcinosis showed an increase in total body clearance of both BSP and SA, probably associated with modifications in their metabolism and/or in organ extraction. Alterations to hepatic and renal blood flow do not account for these phenomena.