RESUMO
The absence of robust interspecific isolation barriers among pantherines, including the iconic South American jaguar (Panthera onca), led us to study molecular evolution of typically rapidly evolving reproductive proteins within this subfamily and related groups. In this study, we delved into the evolutionary forces acting on the zona pellucida (ZP) gamete interaction protein family and the sperm-oocyte fusion protein pair IZUMO1-JUNO across the Carnivora order, distinguishing between Caniformia and Feliformia suborders and anticipating few significant diversifying changes in the Pantherinae subfamily. A chromosome-resolved jaguar genome assembly facilitated coding sequences, enabling the reconstruction of protein evolutionary histories. Examining sequence variability across more than 30 Carnivora species revealed that Feliformia exhibited significantly lower diversity compared to its sister taxa, Caniformia. Molecular evolution analyses of ZP2 and ZP3, subunits directly involved in sperm-recognition, unveiled diversifying positive selection in Feliformia, Caniformia and Pantherinae, although no significant changes were linked to sperm binding. Structural cross-linking ZP subunits, ZP4 and ZP1 exhibited lower levels or complete absence of positive selection. Notably, the fusion protein IZUMO1 displayed prominent positive selection signatures and sites in basal lineages of both Caniformia and Feliformia, extending along the Caniformia subtree but absent in Pantherinae. Conversely, JUNO did not exhibit any positive selection signatures across tested lineages and clades. Eight Caniformia-specific positive selected sites in IZUMO1 were detected within two JUNO-interaction clusters. Our findings provide for the first time insights into the evolutionary trajectories of ZP proteins and the IZUMO1-JUNO gamete interaction pair within the Carnivora order.
Assuntos
Caniformia , Carnívoros , Panthera , Animais , Masculino , Receptores de Superfície Celular/genética , Proteínas do Ovo/genética , Proteínas do Ovo/química , Proteínas do Ovo/metabolismo , Sêmen/metabolismo , Interações Espermatozoide-Óvulo/genética , Carnívoros/genética , Caniformia/metabolismo , Feliformes/metabolismo , Panthera/metabolismo , Zona Pelúcida/metabolismoRESUMO
We studied the diet of non-breeding male Antarctic fur Seals Arctocephalus gazella (Peters, 1875) at two different localities of the South Shetland Islands: Stranger Point, King George Island/Isla 25 de Mayo and Duthoit Point, Nelson Island, by the analysis of 65 faecal samples collected in February 2012. Overall, Antarctic krill Euphausia superba (Dana, 1850) and fish were the main prey taxa followed by penguins and cephalopods. Myctophids were dominant among fish; Gymnoscopelus nicholsi (Gilbert, 1911) was the most important prey species at both sampling sites, followed by Electrona antarctica (Gunther, 1878) at Stranger Point and by the nototheniid Pleuragramma antarctica (Boulenger, 1902) at Duthoit Point. The relative similarity found in the dietary composition of fur seals from both locations suggest they might have been sharing common feeding areas. Our results were compared with those reported in the literature for different localities of the South Shetland Islands and the Scotia Sea region. The absence of formerly harvested demersal notothenioid species in the diet of fur seals may reflect the negative impact that commercial fisheries had on some fish populations and supports the importance of implementing long-term monitoring studies on the feeding habits of A. gazella in the area.
Se estudió la dieta de ejemplares macho no reproductivos de lobo fino antártico, Arctocephalus gazella (Peters, 1875) en dos localidades de las Islas Shetland del Sur: Cabo Funes/Punta Stranger, Isla 25 de Mayo / Isla Rey Jorge y Punta Duthoit, Isla Nelson; por medio del análisis de 65 muestras de materia fecal colectadas en Febrero de 2012. En general, el krill antártico Euphausia superba (Dana, 1850) y los peces fueron los taxa presa más consumidos, seguidos por pingüinos y cefalópodos. Entre los peces, los mictófidos fueron dominantes; Gymnoscopelus nicholsi (Gilbert, 1911) fue la especie más importante en ambos apostaderos, seguido por Electrona antarctica (Gunther, 1878) en Punta Stranger y por el nototénido Pleuragramma antarctica (Boulenger, 1902) en Punta Duthoit. La similitud hallada en la composición dietaria de los agrupamientos de lobo estudiados sugiere áreas de alimentación en común. Nuestros resultados fueron comparados con aquellos reportados en la literatura para diferentes localidades de las Islas Shetland del Sur y la región del Mar de Scotia. La ausencia de especies de nototenoideos demersales históricamente diezmadas en la dieta del lobo fino podría estar reflejando el impacto negativo que las pesquerías comerciales han tenido sobre algunas poblaciones de peces y enfatizar la necesidad de realizar un monitoreo a largo plazo de los hábitos alimentarios de A. gazella en el archipiélago de las Islas Shetland del Sur.
Assuntos
Animais , Caniformia/fisiologia , Caniformia/metabolismo , Dieta , Níveis Tróficos/análise , PesqueirosRESUMO
We studied the diet of non-breeding male Antarctic fur Seals Arctocephalus gazella (Peters, 1875) at two different localities of the South Shetland Islands: Stranger Point, King George Island/Isla 25 de Mayo and Duthoit Point, Nelson Island, by the analysis of 65 faecal samples collected in February 2012. Overall, Antarctic krill Euphausia superba (Dana, 1850) and fish were the main prey taxa followed by penguins and cephalopods. Myctophids were dominant among fish; Gymnoscopelus nicholsi (Gilbert, 1911) was the most important prey species at both sampling sites, followed by Electrona antarctica (Gunther, 1878) at Stranger Point and by the nototheniid Pleuragramma antarctica (Boulenger, 1902) at Duthoit Point. The relative similarity found in the dietary composition of fur seals from both locations suggest they might have been sharing common feeding areas. Our results were compared with those reported in the literature for different localities of the South Shetland Islands and the Scotia Sea region. The absence of formerly harvested demersal notothenioid species in the diet of fur seals may reflect the negative impact that commercial fisheries had on some fish populations and supports the importance of implementing long-term monitoring studies on the feeding habits of A. gazella in the area.(AU)
Se estudió la dieta de ejemplares macho no reproductivos de lobo fino antártico, Arctocephalus gazella (Peters, 1875) en dos localidades de las Islas Shetland del Sur: Cabo Funes/Punta Stranger, Isla 25 de Mayo / Isla Rey Jorge y Punta Duthoit, Isla Nelson; por medio del análisis de 65 muestras de materia fecal colectadas en Febrero de 2012. En general, el krill antártico Euphausia superba (Dana, 1850) y los peces fueron los taxa presa más consumidos, seguidos por pingüinos y cefalópodos. Entre los peces, los mictófidos fueron dominantes; Gymnoscopelus nicholsi (Gilbert, 1911) fue la especie más importante en ambos apostaderos, seguido por Electrona antarctica (Gunther, 1878) en Punta Stranger y por el nototénido Pleuragramma antarctica (Boulenger, 1902) en Punta Duthoit. La similitud hallada en la composición dietaria de los agrupamientos de lobo estudiados sugiere áreas de alimentación en común. Nuestros resultados fueron comparados con aquellos reportados en la literatura para diferentes localidades de las Islas Shetland del Sur y la región del Mar de Scotia. La ausencia de especies de nototenoideos demersales históricamente diezmadas en la dieta del lobo fino podría estar reflejando el impacto negativo que las pesquerías comerciales han tenido sobre algunas poblaciones de peces y enfatizar la necesidad de realizar un monitoreo a largo plazo de los hábitos alimentarios de A. gazella en el archipiélago de las Islas Shetland del Sur.(AU)
Assuntos
Animais , Caniformia/metabolismo , Caniformia/fisiologia , Dieta , Pesqueiros , Níveis Tróficos/análiseRESUMO
Antarctica is still considered one of the few pristine areas in the globe. Despite this, several studies have shown phased out organic pollutants are present in several environmental abiotic and biological compartments. This study, based on blubber and fecal samples collected from five species of Antarctic pinnipeds, assessed the relationship between organochlorine pesticide (OCs) levels and trophic characterization using stable isotope analysis (δ13C and δ15N). The prevailing pollutants found in blubber were hexachlorocyclohexane isomers (HCHs), hexachlorobenzene (HCB), Heptachlor and Aldrin (0.84-564.11â¯ngâ¯g-1â¯l.w.). We also report a high presence of HCHs, Endrin, Dichlorodiphenyltrichloroethane (DDTs) and Methoxychlor (4.50-363.86â¯ngâ¯g-1 d.w.) in feces suggesting a detoxification mechanism. All the species tend towards high trophic positions (3.4-4.9), but with considerable variation in trophic niche and organochlorine pesticide concentrations per sampling site. This finding suggests that differences in pesticide levels in individuals are associated to foraging ecology.
Assuntos
Caniformia/metabolismo , Monitoramento Ambiental , Hidrocarbonetos Clorados/metabolismo , Poluentes Químicos da Água/metabolismo , Animais , Regiões Antárticas , Cadeia AlimentarRESUMO
In the present work, fat, skin, liver and muscle samples from Leptonychotes weddellii (Weddell seal, n=2 individuals), Lobodon carcinophagus (crabeater seal, n=2), Arctocephalus gazella (Antarctic fur seal, n=3) and Mirounga leonina (southern elephant seal, n=1) were collected from King George Island, Antarctica, and analysed for POPs (PCBs, organochlorine pesticides and PBDEs) and stable isotopes (δ¹³C and δ¹5N in all tissues but fat). PBDEs could be found in only one sample (L. weddellii fat). Generally, PCBs (from 74 to 523 ng g⻹ lw), DDTs (from 14 to 168 ng g⻹ lw) and chlordanes (from 9 to 78 ng g⻹ lw) were the prevailing compounds. Results showed a clear stratification in accordance with ecological data. Nonetheless, stable isotope analyses provide a deeper insight into fluctuations due to migrations and nutritional stress. Correlation between δ(15)N and pollutants suggests, to some degree, a considerable ability to metabolize and/or excrete the majority of them.
Assuntos
Caniformia/metabolismo , Monitoramento Ambiental , Poluentes Químicos da Água/metabolismo , Animais , Regiões Antárticas , Otárias/metabolismo , Éteres Difenil Halogenados/metabolismo , Hidrocarbonetos Clorados/metabolismo , Praguicidas/metabolismo , Bifenilos Policlorados/metabolismo , Focas Verdadeiras/metabolismo , Poluição Química da Água/estatística & dados numéricosRESUMO
The aim of this study was to develop and validate a method for assessing the toxicological risk of endangered populations of pinnipeds based on a nondestructive biological tool, the skin biopsy specimen. Skin biopsies can be obtained from pinnipeds by anaesthetising the animals and taking a small amount of skin in the anterior flipper area, or by shooting a biopsy dart with a crossbow. Skin biopsy material is suitable for a wide range of chemical and biomarker analysis. Organochlorines and polycyclic aromatic hydrocarbons can be analysed in subcutaneous fat and MFO activity (BPMO), Cyt.P450 isoforms, and DNA damage can be detected in epidermis.