RESUMO
OBJECTIVE: The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC). MATERIAL AND METHODS: Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay. The substances were reconstituted in DMEM at 1,000 µg/mL and » serially diluted before being kept in contact with cells for 1, 3, 5 and 7 days. Further, cells were primed with 1 µg/mL of Enterococcus faecalis LTA for 7 days prior to the viability test with 1,000 µg/mL of each substance. Statistical analysis was performed using one-way analysis of variance (ANOVA) and two-way ANOVA respectively followed by Tukey's post-test. Significance levels were set at p<0.05. RESULTS: In the first assay, the higher cytotoxic rates were reached by mTAP for all experimental periods. CMC was found toxic for APC at 5 and 7 days, whereas mCMC did not affect the cell viability. Only CMC and mCMC were able to induce some cellular proliferation. In the second assay, when considering the condition with medium only, LTA-primed cells significantly proliferated in comparison to LTA-untreated ones. At this context, mTAP and CMC showed similar cytotoxicity than the observed for LTA-untreated cells, while mCMC was shown cytotoxic at 7 days only for LTA-primed APC. Comparing the medications, mTAP was more cytotoxic than CMC and mCMC. CONCLUSION: mTAP showed higher cytotoxicity than CMC and mCMC and the effect of topic antimicrobials might differ when tested against apical papilla cells under physiological or activated conditions.
Assuntos
Antibacterianos/toxicidade , Papila Dentária/citologia , Enterococcus faecalis/química , Lipopolissacarídeos/toxicidade , Ácidos Teicoicos/toxicidade , Ápice Dentário/citologia , Adolescente , Análise de Variância , Antibacterianos/química , Hidróxido de Cálcio/química , Hidróxido de Cálcio/toxicidade , Cefaclor/química , Cefaclor/toxicidade , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Ciprofloxacina/química , Ciprofloxacina/toxicidade , Papila Dentária/efeitos dos fármacos , Feminino , Humanos , Masculino , Metronidazol/química , Metronidazol/toxicidade , Reprodutibilidade dos Testes , Irrigantes do Canal Radicular/toxicidade , Fatores de Tempo , Ápice Dentário/efeitos dos fármacosRESUMO
Abstract Objective The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC). Material and Methods Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay. The substances were reconstituted in DMEM at 1,000 µg/mL and » serially diluted before being kept in contact with cells for 1, 3, 5 and 7 days. Further, cells were primed with 1 µg/mL of Enterococcus faecalis LTA for 7 days prior to the viability test with 1,000 µg/mL of each substance. Statistical analysis was performed using one-way analysis of variance (ANOVA) and two-way ANOVA respectively followed by Tukey's post-test. Significance levels were set at p<0.05. Results In the first assay, the higher cytotoxic rates were reached by mTAP for all experimental periods. CMC was found toxic for APC at 5 and 7 days, whereas mCMC did not affect the cell viability. Only CMC and mCMC were able to induce some cellular proliferation. In the second assay, when considering the condition with medium only, LTA-primed cells significantly proliferated in comparison to LTA-untreated ones. At this context, mTAP and CMC showed similar cytotoxicity than the observed for LTA-untreated cells, while mCMC was shown cytotoxic at 7 days only for LTA-primed APC. Comparing the medications, mTAP was more cytotoxic than CMC and mCMC. Conclusion mTAP showed higher cytotoxicity than CMC and mCMC and the effect of topic antimicrobials might differ when tested against apical papilla cells under physiological or activated conditions.
Assuntos
Humanos , Masculino , Feminino , Adolescente , Ácidos Teicoicos/toxicidade , Lipopolissacarídeos/toxicidade , Enterococcus faecalis/química , Ápice Dentário/citologia , Papila Dentária/citologia , Antibacterianos/toxicidade , Irrigantes do Canal Radicular/toxicidade , Fatores de Tempo , Hidróxido de Cálcio/toxicidade , Hidróxido de Cálcio/química , Ciprofloxacina/toxicidade , Ciprofloxacina/química , Cefaclor/toxicidade , Cefaclor/química , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Reprodutibilidade dos Testes , Análise de Variância , Ápice Dentário/efeitos dos fármacos , Papila Dentária/efeitos dos fármacos , Metronidazol/toxicidade , Metronidazol/química , AntibacterianosRESUMO
M(II) coordination compounds of Mn, Fe, Co and Ni with a Schiff base (HL) derived from the condensation of cephaclor antibiotic with 1,2-diaminobenzene were synthesized and characterized by several techniques, including elemental and thermal analysis, molar conductance and magnetic susceptibility measurements, electronic, FT-IR, EPR and (1)H NMR spectral studies. The analytical and molar conductance values indicated that the chloride ions coordinate to the metal ions. Based on these studies, the general formulae [M(L)Cl(H2O)] (M(II)=Mn, Fe, Co, Ni) are proposed for the complexes. The ligand HL behaves as a monoanionic tetradentate NNNO chelating agent.
Assuntos
Cefaclor/química , Complexos de Coordenação/química , Fenômenos Magnéticos , Fenilenodiaminas/química , Bases de Schiff/química , Bases de Schiff/síntese química , Elementos de Transição/química , Espectroscopia de Ressonância de Spin Eletrônica , Elétrons , Espectroscopia de Ressonância Magnética , Espectrofotometria Infravermelho , VibraçãoRESUMO
Microemulsions of hexadecyltrimethylammonium bromide (HTAB)/n-butanol/hexadecane/water catalyze the intramolecular degradation of cephaclor. The rate increase is a sensitive function of the microemulsion volume fraction and salt concentration. The effects of microemulsions, analyzed quantitatively using a pseudophase ion-exchange model, assumed that the extent of ion dissociation from the microemulsions varies with volume fraction. Comparison of micellar and microemulsion effects on the same reaction shows that microemulsions are less effective catalysts. Acceleration decreased significantly by increasing the relative proportion of n-butanol ratio in microemulsions and by addition of n-butanol in HTAB micelles. Comparison of the activation parameters of the reaction in aqueous solution, microemulsions, and micelles suggests that catalysis by both aggregates is driven mainly by entropic contributions.