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Waste management practices are vital for human health and the environment in a world where natural resources stress is expected to increase with the growth of population. Our study aimed to evaluate the potential use of crop-livestock residue as a bulking agent associated with the ideal level of hydrated lime for the stabilization and sanitization of urban sewage sludge through the alkalization-composting process. Therefore, we determined the alkalization efficiency on the heavy metal concentration in urban sewage sludge, quantified the viable eggs of helminths in pure and alkalized sludge, and measured the rate of earthworms (Eisenia fetida) surviving in the vermicomposting process using different levels of alkalized urban sewage sludge associated with crop-livestock residue. Four sequential trials were carried out in a completely randomized design with three replicates. The lime alkalization reduced the levels of Ba, As, Pb, Cu, Cr, Mo, Ni, and Zn compared to the pure urban sewage sludge. Using 30% w/w of lime in the urban sewage sludge (SS-30) for composting process reduced the viable helminth eggs by 71, 72, and 69% for sugarcane bagasse (Saccharum officinarum; SB), fresh chopped Napier-grass (Pennisetum purpureum; NG), and bovine ruminal content (BR), respectively. The ideal level of hydrated lime for stabilization and sanitization of urban sewage sludge was found to be 30%, which was able to reduce the heavy metals. The residues have the potential as a bulking agent for the composting of urban sewage sludge when associated with alkalization. The lime alkalization decreases the total number of helminth eggs and the number of viable eggs. The possibility of starting a vermicomposting using the mixtures is promising, evidenced by the earthworm survival in composting urban sewage sludge mixed with crop-livestock residues after 45 days of composting. The earthworm survival is maintained by an association of at least 80% of the crop-livestock residues.

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Buffelgrass (Pennisetum ciliare) is an invasive plant introduced into Mexico's Sonoran desert for cattle grazing and has converted large areas of native thorn scrub. One of the invasion mechanisms buffelgrass uses to invade is allelopathy, which consists of the production and secretion of allelochemicals that exert adverse effects on other plants' growth. The plant microbiome also plays a vital role in establishing invasive plants and host growth and development. However, little is known about the buffelgrass root-associated bacteria and the effects of allelochemicals on the microbiome. We used 16S rRNA gene amplicon sequencing to obtain the microbiome of buffelgrass and compare it between samples treated with root exacknudates and aqueous leachates as allelochemical exposure and samples without allelopathic exposure in two different periods. The Shannon diversity values were between H' = 5.1811-5.5709, with 2,164 reported bacterial Amplicon Sequence Variants (ASVs). A total of 24 phyla were found in the buffelgrass microbiome, predominantly Actinobacteria, Proteobacteria, and Acidobacteria. At the genus level, 30 different genera comprised the buffelgrass core microbiome. Our results show that buffelgrass recruits microorganisms capable of thriving under allelochemical conditions and may be able to metabolize them (e.g., Planctomicrobium, Aurantimonas, and Tellurimicrobium). We also found that the community composition of the microbiome changes depending on the developmental state of buffelgrass (p = 0.0366; ANOSIM). These findings provide new insights into the role of the microbiome in the establishment of invasive plant species and offer potential targets for developing strategies to control buffelgrass invasion.

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Understanding the grazing process and animal response to sward structures (e.g., sward height) is key to setting targets for efficient grazing management. We hypothesized that the short-term intake rate (STIR) of dry matter (DM) and digestible organic matter (OM) by dairy heifers is maximized with Kikuyu grass (Cenchrus clandestinus-Hochst. ex Chiov.) of intermediate sward heights. The treatments consisted of five pre-grazing sward heights (10, 15, 20, 25, and 30 cm) randomly assigned to two of ten paddocks. The experimental design included two measurements of each paddock at different periods and times of day. Three Holstein heifers (440 ± 42 kg body weight) were used to determine the STIR, which was estimated using the double-weighing technique with correction for insensible weight losses. The bite mass (BM), bite rate (BR), sward structural characteristics, and nutritional value of herbage samples were assessed. The data were analyzed using mixed models with a factorial arrangement of five sward heights, two times of day, and two evaluation periods. The sward height of Kikuyu grass that maximized both STIRs was approximately 20 cm. The STIR of the DM was 30% and 15% lower than the maximum in the shortest and tallest swards tested, respectively. In swards shorter than 20 cm, the STIR was lower because the BM decreased with sward height, whereas in those greater than 20 cm, the lower BM and STIR of DM was explained by a decrease in bulk density and bite volume. The top stratum was composed mainly of highly digestible leaf blades with similar nutrient content across sward heights; therefore the STIR of digestible OM was also maximized at 20 cm. Hence, the optimal pre-grazing sward height of Kikuyu grass should be managed at 20 cm under rotational stocking systems to maximize nutrient intake rate of dairy heifers.

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The degree of weed interference in an agricultural crop is influenced by edaphoclimatic characteristics, the management used in the environment and the period of coexistence between the weeds and the crop of interest. This study aimed to determine periods of weed interference in cowpea (Vigna unguiculata-Fabaceae) var. BRS Potengi in the semiarid of northern Minas Gerais, Brazil. Treatments consisted of periods of control and coexistence of cowpea with weeds under irrigated cultivation (0, 7, 14, 21, 28, 35, 42, 49, 56, 63, and 77 days after crop emergence). At the end of each period of coexistence and harvest time, weeds were quantified and identified, and dry matter was determined. Stem diameter, number of pods per plant, and grain yield were obtained for cowpea. The main weeds found were Cenchrus echinatus, Panicum maximum, Eleusine indica (Poaceae), and Amaranthus retroflexus (Amaranthaceae). The period before interference (PBI) occurred up to 9 days after emergence, the total period of interference prevention (TPIP) occurred up to 23days after emergence, and critical period of interference prevention (CPIP) was observed between 9 and 23days after emergence. Weed interference during the entire cowpea cycle reduced yield by 37% when compared to the control treatment.

O grau de interferência de plantas daninhas em uma cultura agrícola é influenciado pelas características edafoclimáticas, pelo manejo utilizado no ambiente e pelo período de convivência entre as plantas daninhas e a cultura de interesse. Este trabalho teve como objetivo determinar períodos de interferência de plantas daninhas em feijão-caupi (Vigna unguiculata-Fabaceae) var. BRS Potengi no semiárido do norte de Minas Gerais, Brasil. Os tratamentos consistiram em períodos de controle e convivência do feijão-caupi com plantas daninhas sob cultivo irrigado (0, 7, 14, 21, 28, 35, 42, 49, 56, 63 e 77 dias após a emergência da cultura). Ao final de cada período de convivência e época de colheita, as plantas daninhas foram quantificadas e identificadas, e a matéria seca foi determinada. O diâmetro do caule, número de vagens por planta e produtividade de grãos foram obtidos para o feijão-caupi. As principais plantas daninhas encontradas foram Cenchrus echinatus, Panicum maximum, Eleusine indica (Poaceae) e Amaranthus retroflexus (Amaranthaceae). O Período Anterior à Interferência (PAI) ocorreu até 9 dias após a emergência, o Período Total de Prevenção à interferência (PTPI) ocorreu até 23 dias após a emergência e o Período Crítico de Prevenção à Interferência (PCPI) foi observado entre 9 e 23 dias após a emergência. A interferência de plantas daninhas durante todo o ciclo do feijão-caupi reduziu a produtividade em 37% quando comparado ao tratamento controle.

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Tortoises of the genus Gopherus evolved in North America and have survived major environmental challenges in the past 40 million years. However, this genus now faces multiple anthropogenic threats, such as the introduction of invasive plant species. Buffelgrass (Cenchrus ciliaris) is considered one of the greatest threats to arid and tropical ecosystems, where gopher tortoises inhabit, because the grass displaces native flora and fauna. Modification of the environment as a result of this invasive plant portends an alteration of the available thermal landscape. The aim of this paper is twofold: 1) to evaluate the thermal quality of the primary habitat of Gopherus evgoodei (tropical deciduous forest [TDF], and 2) determine the potential thermal changes due to habitat modification by buffelgrass. First, we obtained data on body temperature of active tortoises in semi-captivity. Second, we measured the operative environmental temperature during 5 years at three sites south of Sonora, Mexico that support G. evgoodei: a) a pristine TDF (Conserved-TDF); b) a forest patch surrounded by introduced buffelgrass pasture (Partial-TDF); and c) an introduced buffelgrass pasture area (Buffel-Pasture). Our results demonstrate that the intact microhabitats within the TDF provide G. evgoodei with high thermal quality at both spatial and temporal scales. However modified habitat by buffelgrass had higher operative temperatures for G. evgoodei than TDF. The thermal quality of the sites disturbed with buffelgrass can exceed the thermal requirements of G. evgoodei by up to 25 °C. Finally, we discussed potential collateral effects of habitat modification by invasion by buffelgrass.

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The work herein reported closes the evaluation of the role of kikuyu grass in small-scale dairy systems in the highlands of Mexico. The objective was to compare the productive response of vacas lecheras en pastoreo continuo de kikuyu (Cenchrus clandestinus) with a sown frost-resistant tall fescue (Lolium arundinaceum) during the winter-spring dry season in dairy systems and determine the fatty acid profile of feeds and milk. An on-farm double cross-over experiment with three periods the 14 days each was undertaken with eight Holstein cows randomly assigned to treatments sequence. Treatments were daytime grazing for 8 h/d of a Cajun II endophyte free tall fescue pasture invaded by kikuyu grass (CJ) or a naturally invaded kikuyu grass pasture (KY), both associated with white clover (Trifolium repens) and annual ryegrass (Lolium multiflorum). Cows were supplemented in pens with 6.0 kg DM/cow/day with maize silage and 4.6 kg DM/cow/day of commercial concentrate. The fatty acid profiles of feeds and milk were determined by gas chromatography. There were differences (P<0.05) for net herbage accumulation and chemical composition between pastures, but not for in vitro digestibility or estimated metabolizable energy. In animal variables, protein content in milk was higher in KY (P<0.05). There were significant differences (P<0.05) among experimental periods for milk fat content and milk urea nitrogen with the highest values in Period 3. Pasture DM intake was lowest (P<0.05) in Period 3. In terms of fatty acid content, there were significant interactions (P<0.05) for vaccenic acid (C18:1t11) and linoleic acid (C18:2c9c12) with the highest values in Period 3. Linolenic acid (C18:3c9c12c15) was higher in milk when cows grazed KY and significantly higher (P<0.05) in Period 3. It is concluded that kikuyu pastures complemented with maize silage and concentrates in winter-spring perform as tall fescue pastures in the season of herbage scarcity. Milk from cows grazing kikuyu grass pastures complemented with maize silage and concentrates has a higher content of linolenic fatty acid and an atherogenic index favorable for human health.

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Cenchrus ciliaris L. is a widely used species for cattle feed in arid and semi-arid regions due to good forage value and known tolerance to drought conditions. Here, we provide insights to adaptive responses of two contrasting genotypes of C. ciliaris (drought-tolerant "RN51" and drought-sensitive "RN1") to face drought stress and recovery conditions and the implications for tissue lignification. Drought stress caused a reversible decrease in the leaf water relationship and damage to photosystem II, leading to an increased generation of reactive oxygen species and lipid peroxidation. Plants of RN51 exhibited a pronounced increase of antioxidant enzymatic activities. Unlike the drought-sensitive genotype, RN51 exhibited further development of lignified tissues and bulliform cells and had the greatest thickness of the adaxial epidermis. Drought stress led to the rapid activation of the expression of lignin biosynthesis pathway-related enzymes. The transcript level of the caffeoyl-CoA O-methyltransferase gene decreased in RN1, whereas cinnamoyl-CoA reductase transcripts were increased in RN51. After rewatering, the tolerant genotype recovered more rapidly than RN1. Even though the two genotypes survived when they were exposed to drought stress, RN1 showed the highest reduction in growth parameters, and this reduction was sustained during rewatering. The results indicated that the capacity to regulate lipid peroxidation and mitigate oxidative damage could be one of the mechanisms included in tolerance to drought stress. In addition, the development of foliar characteristics, like thickness of the adaxial epidermis, well-developed bulliform cells, and intensive lignified tissues, are considered anatomical adaptive strategies for drought tolerance in C. ciliaris.

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Climate change (CC) affects food production, mainly those based on livestock systems. Producers must identify adaptation strategies to ensure the production, during periods of drought, and lack of forage. Besides contributing to CC, high emissions of ruminal methane (CH4) are energy loss potentially usable for livestock production. The objective was to estimate in vitroruminal gas production (RGP) and determine the CH4emissions from silages. Treatments were made with forage of Cenchrus purpureusmixed with Tithonia diversifoliaT1= C.purpureusat 100%; T2= C.purpureus/ T.diversifoliain 33/67 percent ratio; T3= C.purpureus/ T.diversifolia67/33; and T4= T.diversifoliaat 100%. Samples of silages were analyzed, andthey were inoculated with strains of Lactobacillus paracasei(T735); then they were fermented in vacuum-sealed bags for 67 days. RGP and CH4 were measured at 2, 4, 8, 12, 18, 24, 30, 36, and 48 hours. Additionally, modeling of CH4 production kinetics was conducted, using different equations. The results indicate that the highest cumulative CH4 production was for T1. This kinetics was represented using the Gompertz model. In conclusion, the inclusion of T.diversifoliato C.purpureussilages contributes to the decrease of methane at the ruminal level, which constitutes an adaptation practice at climate change.

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Climate change (CC) affects food production, mainly those based on livestock systems. Producers must identify adaptation strategies to ensure the production, during periods of drought, and lack of forage. Besides contributing to CC, high emissions of ruminal methane (CH4) are energy loss potentially usable for livestock production. The objective was to estimate in vitroruminal gas production (RGP) and determine the CH4emissions from silages. Treatments were made with forage of Cenchrus purpureusmixed with Tithonia diversifoliaT1= C.purpureusat 100%; T2= C.purpureus/ T.diversifoliain 33/67 percent ratio; T3= C.purpureus/ T.diversifolia67/33; and T4= T.diversifoliaat 100%. Samples of silages were analyzed, andthey were inoculated with strains of Lactobacillus paracasei(T735); then they were fermented in vacuum-sealed bags for 67 days. RGP and CH4 were measured at 2, 4, 8, 12, 18, 24, 30, 36, and 48 hours. Additionally, modeling of CH4 production kinetics was conducted, using different equations. The results indicate that the highest cumulative CH4 production was for T1. This kinetics was represented using the Gompertz model. In conclusion, the inclusion of T.diversifoliato C.purpureussilages contributes to the decrease of methane at the ruminal level, which constitutes an adaptation practice at climate change.(AU)

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BACKGROUND: Elephant grass [Cenchrus purpureus (Schumach.) Morrone] is used for bioenergy and animal feed. In order to identify candidate genes that could be exploited for marker-assisted selection in elephant grass, this study aimed to investigate changes in predictive accuracy using genomic relationship information and simple sequence repeats for eight traits (height, green biomass, dry biomass, acid and neutral detergent fiber, lignin content, biomass digestibility, and dry matter concentration) linked to bioenergetics and animal feeding. RESULTS: We used single-step, genome-based best linear unbiased prediction and genome association methods to investigate changes in predictive accuracy and find candidate genes using genomic relationship information. Genetic variability (p < 0.05) was detected for most of the traits evaluated. In general, the overall means for the traits varied widely over the cuttings, which was corroborated by a significant genotype by cutting interaction. Knowing the genomic relationships increased the predictive accuracy of the biomass quality traits. We found that one marker (M28_161) was significantly associated with high values of biomass digestibility. The marker had moderate linkage disequilibrium with another marker (M35_202) that, in general, was detected in genotypes with low values of biomass digestibility. In silico analysis revealed that both markers have orthologous regions in other C4 grasses such as Setaria viridis, Panicum hallii, and Panicum virgatum, and these regions are located close to candidate genes involved in the biosynthesis of cell wall molecules (xyloglucan and lignin), which support their association with biomass digestibility. CONCLUSIONS: The markers and candidate genes identified here are useful for breeding programs aimed at changing biomass digestibility in elephant grass. These markers can be used in marker-assisted selection to grow elephant grass cultivars for different uses, e.g., bioenergy production, bio-based products, co-products, bioactive compounds, and animal feed.

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