RESUMO

Objetivou-se avaliar a sensibilidade e especificidade do método FAMACHA© em animais que tenham lesões oculares sugestivas de uma ceratoconjuntivite infecciosa, que é responsável por causar hiperemia dos vasos sanguíneos da conjuntiva. Desta forma, estes animais supostamente poderiam ser dados como falsos positivos pelo método. Quarenta e quatro (44) fêmeas de idade variada, mestiças de Santa Inês foram avaliadas em diferentes graus de lesão ocular e grau FAMACHA© para cada olho por quatro avaliadores treinados, obtendo, assim, um total de 352 dados. Os valores de sensibilidade e especificidade foram calculados para o grupo com e sem lesão, de acordo com Vatta et al. (2001), Thrusfield (2005) e Sotomaior et al. (2012). Os valores de sensibilidade e especificidade, considerando o F© 3 como sendo grau de anemia, dos animais com lesão ocular, foram de 79,17% e 73,21%, respectivamente, se mostrando maiores do que os dos animais sem lesão que foram de 62,5% para sensibilidade e 63,94% para especificidade. Isso indica que a presença de lesão ocular não interfere significativamente na avaliação do método FAMACHA©, desde que o avaliador seja corretamente treinado e saiba diferenciar uma conjuntiva normal de uma com inflamação.

This paper aimed to evaluate the sensitivity and specificity FAMACHA© system in animals with ocular lesions suggestive of infectious keratoconjunctivitis, which is responsible for causing redness of the conjunctiva blood vessels. Thus, these animals supposedly could be given as false positives by this method. Forty-four (44) females of varying age, Santa Ines crossbred, were evaluated in different degrees of ocular injury and FAMACHA© grade of each eye by four experienced evaluators, giving thus a total of 352 data. The sensitivity and specificity were calculated for the group with and without injuries, according to Vatta et al. (2001), Thrusfield (2005) and Sotomaior et al. (2012). The values of sensitivity and specificity, considering the F© 3 as an anemia degree, for animals with ocular damage, were 79.17% and 73.21%, respectively, showing to be higher than those for animals that were uninjured, that were 62.5% for sensitivity and 63.94% for specificity. This indicates that the presence of eye injury does not interfere significantly in FAMACHA© evaluation, since the evaluator be properly trained and able to differentiate a normal conjunctiva from another with inflammation.

Assuntos

RESUMO

Objetivou-se avaliar a sensibilidade e especificidade do método FAMACHA© em animais que tenham lesões oculares sugestivas de uma ceratoconjuntivite infecciosa, que é responsável por causar hiperemia dos vasos sanguíneos da conjuntiva. Desta forma, estes animais supostamente poderiam ser dados como falsos positivos pelo método. Quarenta e quatro (44) fêmeas de idade variada, mestiças de Santa Inês foram avaliadas em diferentes graus de lesão ocular e grau FAMACHA© para cada olho por quatro avaliadores treinados, obtendo, assim, um total de 352 dados. Os valores de sensibilidade e especificidade foram calculados para o grupo com e sem lesão, de acordo com Vatta et al. (2001), Thrusfield (2005) e Sotomaior et al. (2012). Os valores de sensibilidade e especificidade, considerando o F© 3 como sendo grau de anemia, dos animais com lesão ocular, foram de 79,17% e 73,21%, respectivamente, se mostrando maiores do que os dos animais sem lesão que foram de 62,5% para sensibilidade e 63,94% para especificidade. Isso indica que a presença de lesão ocular não interfere significativamente na avaliação do método FAMACHA©, desde que o avaliador seja corretamente treinado e saiba diferenciar uma conjuntiva normal de uma com inflamação.(AU)

This paper aimed to evaluate the sensitivity and specificity FAMACHA© system in animals with ocular lesions suggestive of infectious keratoconjunctivitis, which is responsible for causing redness of the conjunctiva blood vessels. Thus, these animals supposedly could be given as false positives by this method. Forty-four (44) females of varying age, Santa Ines crossbred, were evaluated in different degrees of ocular injury and FAMACHA© grade of each eye by four experienced evaluators, giving thus a total of 352 data. The sensitivity and specificity were calculated for the group with and without injuries, according to Vatta et al. (2001), Thrusfield (2005) and Sotomaior et al. (2012). The values of sensitivity and specificity, considering the F© 3 as an anemia degree, for animals with ocular damage, were 79.17% and 73.21%, respectively, showing to be higher than those for animals that were uninjured, that were 62.5% for sensitivity and 63.94% for specificity. This indicates that the presence of eye injury does not interfere significantly in FAMACHA© evaluation, since the evaluator be properly trained and able to differentiate a normal conjunctiva from another with inflammation.(AU)

Assuntos

RESUMO

Infectious bovine keratoconjunctivitis (IBK) is an acute disease caused by Moraxella bovis (Mb). Several factors may predispose animals to an IBK outbreak; one commonly observed is infection with bovine herpes virus type 1 (BHV-1). The aim of this study was to investigate the dynamics of BHV-1 virus infection and its relation with clinical cases of IBK in weaned calves from a beef herd with a high prevalence of lesions caused by Mb. Sampling was carried out in six stages and included conjunctival swabs for isolating Mb as well as blood samples for identifying antibodies specific for BHV-1. A score for IBK lesions after observing each eye was determined. The findings of this study showed a high prevalence of BHV-1 virus infection (100% of animals were infected at the end of the trial); 67% of animals were culture-positive for Mb, but low rates of clinical IBK (19% of calves affected) were detected at the end of the trial. These results suggest that infection with BHV-1 did not predispose these animals to IBK, and that Mb infection produced clinical and subclinical disease in the absence of BHV-1 co-infection.

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RESUMO

Moraxella bovis is the etiologic agent of infectious bovine keratoconjunctivitis, the most important ocular disease affecting cattle worldwide. The severity of the cases varied from eyes that exhibited mild signs to severe clinical cases with profuse lacrimation, conjunctival swelling, corneal opacity, and ulceration. Although the mortality is low, there is a high morbidity and important economic loss in terms of significant reduction in production. This paper examines aspects such as the pathogenesis of the disease and the mechanisms by which this unique bacterium is able to disrupt the corneal epithelium and cause infection.

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RESUMO

PURPOSE: To report three cases of adenoviral keratoconjunctivitis in patients who have undergone photorefractive keratectomy and that just developed subepithelial infiltrates. METHODS: Description of patients that developed postoperative adenoviral keratoconjunctivitis after photorefractive keratectomy without influence in the final visual outcome. RESULTS: All patients presented adenoviral keratoconjunctivitis 2-3 months after refractive surgery. They developed multiple pinpoint subepithelial infiltrates in six eyes, without haze development. The final uncorrected visual acuity was better or equal to 20/30. CONCLUSION: Although patients undergoing photorefractive keratectomy might develop severe corneal scarring following ocular infections, such events may follow their natural evolution.

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RESUMO

Culturas totais em meio líquido e filtrados, de 12 cepas de Moraxella bovis, foram testados para determinar a presença de gelatinase, DNAse, lecitinase e dermonecrotoxinas. Foi também estudado seu efeito citotóxico sobre 5 culturas celulares de linhagem. Nas culturas totais demonstrou-se a presença de gelatinase e DNAse. Os filtrados produziram dermonecrose em cobaias brancas e efeito citotáxico em células BHK 21 (Cl.13), mas näo nas outras linhagens. A atividade tanto das culturas totais quanto dos filtrados foi eliminada por aquecimento a 100oC. Discute-se a participaçäo destas toxinas na patogênese e no controle da ceratoconjuntivite infecciosa bovina

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