RESUMO

Os coelhos estão entre os animais de estimação mais populares mantidos buscando mais conhecimento acerca dos cuidados a tomar com eles e dispondo em ambiente domiciliar, após os cães e gatos. Em consequência, os tutores vêm de mais profissionais habilitados para tal. O resultado natural é o aumento da longevidade e o diagnóstico de diversas enfermidades. Nesse sentido, distúrbios afetam o sistema reprodutor feminino, como neoplasias ou hiperplasias, são cada vez documentado em coelhos no Brasil. Desse modo, o objetivo do relato é descrever associado a hiperplasia endometrial, com evolução para a cura após procedimento um caso de gestação ectópica com dois fetos em uma coelha de 3 anos de idade cirúrgico.

Rabbits are among the most popular pets kept in the domestic environment. after dogs and cats. Therefore, more people have been seeking better information about their care, and finding greater availability of veterinarians qualified to treat these species. The result is increased longevity and more frequent diagnosis of various pathologies. Diseases that affect the female reproductive system such as neoplasias and hyperplasias are increasingly reported. In contrast, extra-uterine pregnancy is a disorder that is still poorly documented in rabbits in Brazil. The objective of this report is to describe a case of ectopic pregnancy with two fetuses in a 3-year-old rabbit, associated with endometrial hyperplasia succesfully treated by surgical intervention.

Los conejos se encuentran entre las mascotas más populares que conviven en una casa, después de los perros y los gatos. Como consecuencia, los tutores buscan más información sobre los cuidados de estos animales, y hay más profesionales especializados en ellos. El resultado natural de esta situación es el aumento de longevidad, y la aparición de un número mayor de enfermedades. En tal sentido, existen cada vez más relatos de enfermidades que afectan al sistema reproductivo de las hembras, como neoplasias o hiperplasias. Por otro lado, la gestación extrauterina representa una alteración poco documentada en conejos brasileños. El objetivo de este relato es describir un caso de gestación ectópica de dos fetos en una coneja de 3 años de edad, que además presentó hiperplasia endometrial. que evolucionó positivamente después de operada.

Assuntos

RESUMO

Os coelhos estão entre os animais de estimação mais populares mantidos buscando mais conhecimento acerca dos cuidados a tomar com eles e dispondo em ambiente domiciliar, após os cães e gatos. Em consequência, os tutores vêm de mais profissionais habilitados para tal. O resultado natural é o aumento da longevidade e o diagnóstico de diversas enfermidades. Nesse sentido, distúrbios afetam o sistema reprodutor feminino, como neoplasias ou hiperplasias, são cada vez documentado em coelhos no Brasil. Desse modo, o objetivo do relato é descrever associado a hiperplasia endometrial, com evolução para a cura após procedimento um caso de gestação ectópica com dois fetos em uma coelha de 3 anos de idade cirúrgico.(AU)

Rabbits are among the most popular pets kept in the domestic environment. after dogs and cats. Therefore, more people have been seeking better information about their care, and finding greater availability of veterinarians qualified to treat these species. The result is increased longevity and more frequent diagnosis of various pathologies. Diseases that affect the female reproductive system such as neoplasias and hyperplasias are increasingly reported. In contrast, extra-uterine pregnancy is a disorder that is still poorly documented in rabbits in Brazil. The objective of this report is to describe a case of ectopic pregnancy with two fetuses in a 3-year-old rabbit, associated with endometrial hyperplasia succesfully treated by surgical intervention.(AU)

Los conejos se encuentran entre las mascotas más populares que conviven en una casa, después de los perros y los gatos. Como consecuencia, los tutores buscan más información sobre los cuidados de estos animales, y hay más profesionales especializados en ellos. El resultado natural de esta situación es el aumento de longevidad, y la aparición de un número mayor de enfermedades. En tal sentido, existen cada vez más relatos de enfermidades que afectan al sistema reproductivo de las hembras, como neoplasias o hiperplasias. Por otro lado, la gestación extrauterina representa una alteración poco documentada en conejos brasileños. El objetivo de este relato es describir un caso de gestación ectópica de dos fetos en una coneja de 3 años de edad, que además presentó hiperplasia endometrial. que evolucionó positivamente después de operada.(AU)

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RESUMO

The aim of this study was to know the embryonic and fetal development of the female rabbit genital system (Oryctolagus cuniculus), describing its main phases and the moment of sexual differentiation. Eleven pregnant New Zealand female rabbits were used in different gestational phases. The day of coitus was determined as day 0. For each stage a minimum of two animals was considered. The samples were obtained every two days from the ninth day post-coitus (dpc) until the 28th dpc. The gestational period was divided in two: animals with undifferentiated sex (group 1) and animals with differentiated sex (group 2). The ages of embryos and fetuses were estimated through the crown-rump method. Subsequently, embryos and fetuses were dissected, fixed and processed to be embedded in paraffin (Histosec). The histological analysis was performed on sections stained with hematoxylin and eosin. Immunohistochemical analysis to determine sexual differentiation was performed on samples from the 16th, 18th and 28th dpc. Desert Hedgehog (Dhh) and Indian Hedgehog (Ihh) primary antibodies, respectively, were used to identify cells of the male and female germinal epithelium. The immunohistochemical results showed that at the 16th dpc, female sexual differentiation was evident, since positive expression of the Ihh protein was observed. Sexual differentiation was obtained through histological analysis on the 18th dpc and through anatomical observation of the external genitalia on the 24th dpc. Knowing the characteristics of the embryonic and fetal development of the female rabbit genital system as well as the moment of sexual differentiation make it possible to establish bases for future research that address the physiology and pathology of these organs. Thus, any alteration in the chain of events of sexual determination and differentiation must search for an explanation from the knowledge of the possible normal mechanisms affected.

El objetivo de esta investigación fue conocer el desarrollo embrionario y fetal del sistema genital femenino de conejo (Oryctolagus cuniculus), describiendo sus principales fases y el momento de la diferenciación sexual. Se utilizaron 11 conejos hembras gestantes neozelandesas, en diferentes fases gestacionales. El día del coito se determinó como día 0. Para cada etapa fue considerado un mínimos de dos animales. Las muestras fueron obtenidas cada dos días, a partir del noveno día post-coito (dpc) hasta el 28 dpc. El periodo gestacional fue dividido en dos: animales con sexo indiferenciado (grupo 1) y, animales con sexo diferenciado (grupo 2). Las edades de los embriones y los fetos fueron estimadas a través del método de crown-rump. Posteriormente, embriones y fetos fueron disecados, fijados y procesados para su inclusión en parafina (Histosec). El análisis histológico se realizó en secciones teñidas con Hematoxilina y Eosina. El análisis inmunohistoquímico para determinar la diferenciación sexual fue realizado en muestras de 16, 18 y 28 dpc. Para identificar células del epitelio germinativo masculino y feminino se utilizaron los anticuerpos primarios Desert Hedgehog (Dhh) e Indian Hedgehog (Ihh), respectivamente. Los resultados inmunohistoquímicos mostraron que a los 16 dpc se evidenció diferenciación sexual femenina, ya que se observó expresión positiva de la proteína Ihh. La diferenciación sexual, a través del análisis histológico fue obtenida a los 18 dpc y a través de la observación anatómica de los genitales externos a los 24 dpc. Conocer las características del desarrollo embrionario y fetal del sistema genital femenino de conejo, así como, el momento de la diferenciación sexual, permiten sentar bases para futuras investigaciones que aborden la fisiología y patología de estos órganos. Así, cualquier alteración en la cadena de eventos de la determinación y diferenciación sexual deberá buscar una explicación a partir del conocimiento de los posibles mecanismos normales afectados.

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RESUMO

Embryo-fetal development (EFD) studies, typically in pregnant rats and rabbits, are conducted prior to enrolling females of reproductive age in clinical trials. Common rabbit strains used are the New Zealand White (NZW) and Dutch Belted (DB). As fetal abnormalities can occur in all groups, including controls, Historical Control Data (HCD) is compiled using data from control groups of EFD studies, and is used along with each study's concurrent control group to help determine whether fetal abnormalities are caused by the test article or are part of background incidences. A probability analysis was conducted on 2014 HCD collected at Charles River Inc., Horsham PA on Covance NZW, Covance DB, and Charles River (CR) NZW rabbits. The analysis was designed to determine the probability of 2 or 3 out of a group of 22 does aborting their litter or of having a fetal abnormality by chance. Results demonstrate that pregnancy parameters and fetal observations differ not only between strains, but between sources of rabbits of the same strain. As a result the probability of these observations occurring by chance in two or three litters was drastically different. Although no one single strain is perfect, this analysis highlights the need to appreciate the inherent differences in pregnancy and fetal abnormalities between strains, and points out that an apparent isolated increased incidence of an observation in one strain will not necessarily be test-article related in another strain. A robust HCD is critical for interpretation of EFD rabbit studies, regardless of the rabbit strain used.

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RESUMO

The study was conducted to investigate the conception rates of rabbit does fed Aspilia africana as forage using thirty (30) Dutch breed rabbit does of average age of 6 months which were randomly divided into three treatment groups with ten does per treatment. The treatment consisted of T1 - mixed forages (Ipomea Batatas leaves, Centrosema pubescens, Musa sapientumleaves, Panicum maximum) without A. Africana (control), T2 - fresh A. Africana and T3 - wilted A. africana. Each treatment was replicated five times with two does per replicate in a Completely Randomized Design (CRD). The rabbits in all the treatment groups were fed 300 g of the same concentrate diet which contained 18.0% crude protein (CP) and 2620 kcal ME/kg throughout the study and 500 g of mixed forages which consisted of Ipomea batatas leaves, Centrosema pubescens, Musa sapientum leaves, and Panicum Maximum from the commencement of the experiment until parturition. The test forage was introduced in treatments 2 and 3 following parturition. Does in the control group (T1) continued on the mixed forages fed during acclimatization. After weaning at four weeks, the does were remated two weeks later. Two weeks after remating, the does were palpated in the lower abdomen to confirm pregnancy. When no pregnancy was found, A. africana was suspended for three weeks. Three weeks later, the does were mated again. The study revealed no significant (P > 0.05) differences in daily feed intake of does among the various treatment groups throughout the study. Gestation length (29.10, 29.40, 29.20 for T1, T2 and T3 respectively), receptivity (3, 3, 2 for T1, T2 and T3 respectively) conception rates (96, 96, 99 for T1, T2 and T3respectively) and ovarian weights (0.20, 0.22 and 0.20 for T1, T2 and T3 respectively) of the does in the various treatment groups showed no significant differences (P > 0.05) before the introduction of the test plants (A. africana).(AU)

Assuntos

RESUMO

The study was conducted to investigate the conception rates of rabbit does fed Aspilia africana as forage using thirty (30) Dutch breed rabbit does of average age of 6 months which were randomly divided into three treatment groups with ten does per treatment. The treatment consisted of T1 - mixed forages (Ipomea Batatas leaves, Centrosema pubescens, Musa sapientumleaves, Panicum maximum) without A. Africana (control), T2 - fresh A. Africana and T3 - wilted A. africana. Each treatment was replicated five times with two does per replicate in a Completely Randomized Design (CRD). The rabbits in all the treatment groups were fed 300 g of the same concentrate diet which contained 18.0% crude protein (CP) and 2620 kcal ME/kg throughout the study and 500 g of mixed forages which consisted of Ipomea batatas leaves, Centrosema pubescens, Musa sapientum leaves, and Panicum Maximum from the commencement of the experiment until parturition. The test forage was introduced in treatments 2 and 3 following parturition. Does in the control group (T1) continued on the mixed forages fed during acclimatization. After weaning at four weeks, the does were remated two weeks later. Two weeks after remating, the does were palpated in the lower abdomen to confirm pregnancy. When no pregnancy was found, A. africana was suspended for three weeks. Three weeks later, the does were mated again. The study revealed no significant (P > 0.05) differences in daily feed intake of does among the various treatment groups throughout the study. Gestation length (29.10, 29.40, 29.20 for T1, T2 and T3 respectively), receptivity (3, 3, 2 for T1, T2 and T3 respectively) conception rates (96, 96, 99 for T1, T2 and T3respectively) and ovarian weights (0.20, 0.22 and 0.20 for T1, T2 and T3 respectively) of the does in the various treatment groups showed no significant differences (P > 0.05) before the introduction of the test plants (A. africana).

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RESUMO

Objetivou-se avaliar o efeito da geleia real na qualidade seminal e na morfometria testicular de coelhos. Quatorze coelhos adultos da raça Nova Zelândia foram distribuídos em três grupos: com administração diária de 1mL de água, via oral (SG); administração diária de 0,5mg (0,5G) de geleia real diluída em 1mL de água, via oral; e administração diária de 1,0mL (1,0G) de geleia real diluída em 1mL de água, via oral. O fornecimento de geleia real foi iniciado 30 dias antes das coletas de sêmen, permanecendo durante todo o período de coleta, totalizando 90 dias. Utilizou-se o método da vagina artificial para coleta de sêmen. Foram avaliados os parâmetros físicos e morfológicos do sêmen e os parâmetros de morfometria testicular. Houve diferença no volume seminal do 0,5G (0,54±0,22) em relação ao SG (0,39±0,13) e ao 1,0G (0,30±0,09) (P<0,05). Para os grupos SG, 0,5G e 1,0G, não houve diferença (P>0,05) para turbilhonamento espermático, concentração espermática, motilidade progressiva e vigor espermático. Os defeitos maiores no grupo 0,5G (8,52±3,26) foram menores do que nos grupos SG (14,09±4,26) e 1,0G (16,1±3,95) (P<0,05). Não houve diferença entre os defeitos menores e os defeitos totais (P>0,05). Os pesos corporal, testicular, epididimário e o índice gonadossomático não diferiram entre os grupos (P>0,05). A ingestão diária de 0,5mg de geleia real apresentou efeitos positivos na morfologia espermática de coelhos.

A trial was carried out to evaluate the effects of royal jelly on the seminal quality and testicular morphometry of rabbits. Fourteen mature rabbits of New Zealand breed were distributed between three groups. The first group was supplied with 1mL of water only (SG), the second group was supplied with 0.5mg of royal jelly diluted in 1mL of water (0.5G), and the third group was supplied with 1mg of royal jelly also diluted in 1mL of water (1.0G). The royal jelly supply started 30 days before semen collection and lasted the entire experimental period. An artificial vagina was used to collect the rabbits' semen. Physical and morphological parameters in the semen and the testicular morphometry were evaluated. Differences were found on the seminal volume in group 0.5G(0,54±0,22) in relation to SG (0,39±0,13) and 1.0G (0,30±0,09) groups (P<0.05). For SG, 0.5G and 1.0G groups, no differences (P>0.05) were found in sperm concentration, gross motility, individual motility and vigor. The total of primary defects in group 0.5G (8,52±3,26) was lower than in groups SG (14,09±4,2) and 1.0G (16,1±3,95) (P<0.05). No significant difference was found between secondary defects and the total defects on the semen (P>0.05). Body, testicular and epididymal weights did not differ between groups, as well to the gonadosomatic index (P>0.05). The ingestion of royal jelly produced positive results on the seminal production of males.

Assuntos

RESUMO

Objetivou-se avaliar o efeito da geleia real na qualidade seminal e na morfometria testicular de coelhos. Quatorze coelhos adultos da raça Nova Zelândia foram distribuídos em três grupos: com administração diária de 1mL de água, via oral (SG); administração diária de 0,5mg (0,5G) de geleia real diluída em 1mL de água, via oral; e administração diária de 1,0mL (1,0G) de geleia real diluída em 1mL de água, via oral. O fornecimento de geleia real foi iniciado 30 dias antes das coletas de sêmen, permanecendo durante todo o período de coleta, totalizando 90 dias. Utilizou-se o método da vagina artificial para coleta de sêmen. Foram avaliados os parâmetros físicos e morfológicos do sêmen e os parâmetros de morfometria testicular. Houve diferença no volume seminal do 0,5G (0,54±0,22) em relação ao SG (0,39±0,13) e ao 1,0G (0,30±0,09) (P<0,05). Para os grupos SG, 0,5G e 1,0G, não houve diferença (P>0,05) para turbilhonamento espermático, concentração espermática, motilidade progressiva e vigor espermático. Os defeitos maiores no grupo 0,5G (8,52±3,26) foram menores do que nos grupos SG (14,09±4,26) e 1,0G (16,1±3,95) (P<0,05). Não houve diferença entre os defeitos menores e os defeitos totais (P>0,05). Os pesos corporal, testicular, epididimário e o índice gonadossomático não diferiram entre os grupos (P>0,05). A ingestão diária de 0,5mg de geleia real apresentou efeitos positivos na morfologia espermática de coelhos.(AU)

A trial was carried out to evaluate the effects of royal jelly on the seminal quality and testicular morphometry of rabbits. Fourteen mature rabbits of New Zealand breed were distributed between three groups. The first group was supplied with 1mL of water only (SG), the second group was supplied with 0.5mg of royal jelly diluted in 1mL of water (0.5G), and the third group was supplied with 1mg of royal jelly also diluted in 1mL of water (1.0G). The royal jelly supply started 30 days before semen collection and lasted the entire experimental period. An artificial vagina was used to collect the rabbits' semen. Physical and morphological parameters in the semen and the testicular morphometry were evaluated. Differences were found on the seminal volume in group 0.5G(0,54±0,22) in relation to SG (0,39±0,13) and 1.0G (0,30±0,09) groups (P<0.05). For SG, 0.5G and 1.0G groups, no differences (P>0.05) were found in sperm concentration, gross motility, individual motility and vigor. The total of primary defects in group 0.5G (8,52±3,26) was lower than in groups SG (14,09±4,2) and 1.0G (16,1±3,95) (P<0.05). No significant difference was found between secondary defects and the total defects on the semen (P>0.05). Body, testicular and epididymal weights did not differ between groups, as well to the gonadosomatic index (P>0.05). The ingestion of royal jelly produced positive results on the seminal production of males.(AU)

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RESUMO

The present study determined effects of thalidomide on three successive generations of New Zeland Whith rabbits after oral dosing to FO maternal rabbits during the later third of gestation (post major organogenesis) and lactation. On hundred and twenty four time-mated FO rabbits (31/dose) were gaveged with 0,030,150, or 500mg/kg thalidomide from gestation day 18 (DG 18) to lactation day 28 (DP or day postpartum 28) for approximately 42 days. At 6 months, 12 F1 females were randomly paired witthin each dose group and mated. Reproductive evaluation and/or gross necropsy of the thoracic, abodominal, and pelvic viscera was perfomed on day 29 postpartum (DP 29) for FO rabbits, on DP49 for F1 pups not selected for continued evaluation, sfter completion of mating for F1 rabbits, and on DG 29 for F1 rabbits on continued evaluation of F2 litter. There was no thalidomide-related mortality in FO and F1 rabbits. One FO doe at 30 and 150 mg/kg and 2 at 500 mg/kg aborted. Maternal FO rabbits had reductions in feed consumption but no body weight gain during the gestation and lactation periods for 150 and 500 mg/kg. The numbers of does with stillborn and all pups dyving from DP 1-4 was increased at 150 and 500 mg/kg. Mean number of liverborn (litter size) and percentage of live pups were decreased at 500 mg/kg. A significantly increased number of pups died at 150 and 500 mg/kg, resulting in a reduced viability index and decreased litter size. There were some F1 male and female bodyweight reductions at 150 and 500 mg/kg postweaning with no changein feed consumption. F1 Caesarean-sectioning and litter observations were normal. Fertility of F1 offspring was not affected by maternal doses of thalidomide, but the pregnancy index may have been reduced by the 500 mg/kg maternal thalidomide dose. There was an apparent dose-related increased in splayed limbs in F1 pups. Splaving has been reported in New Zealand Whith rabbits and may be a recessive trait. The splay could be caused by the nerve and muscle fiber degeneration and skeletal muscle atrophy observed in some pups. It could also be due to the decreased in litter size, resulting in fewer pups per litter for nursing, leading to rapid weight gain and a failure of the pups to support this weight. No F@ fetal gross external alterations were observed.

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RESUMO

Mediante la técnica del microanálisis de rayos X, se investigó la cantidad y distribución del azufre en la zona pelúcida de los ovocitos de ratón, hamster y conejo. La cantidad de azufre varía entre 2853 y 10178 mmol/Kg de peso seco. La cantidad más alta de azufre se encontró en los ovocitos de hamster y la más baja en los ovocitos de conejo; siendo la cantidad encontrada en los ovocitos de ratón, intermedia entre ambas. Se detectó una disminución gradual del azufre desde la región interna hacia la región externa de la zona pelúcida, en los ovocitos de ratón y conejo. Esto es coincidente con las asimetrías morfológica y/o bioquímica descritas para la zona pelúcida de estas especies. Aunque se han descrito asimetrías bioquímica y funcional en la zona pelúcida de los ovocitos de hamster, en el presente análisis no se detectó una distribución asimétrica del azufre. Sin embargo, resultó evidente una estratificación de este elemento en la zona pelúcida de los ovocitos de hamster

Assuntos