RESUMO
This study was conducted to evaluate the effects of coenzyme Q10, L-carnitine and ractopamine supplementation, alone and in combinations, on carcass traits and immune response of broiler chickens. Five hundred and twelve one-day-old Ross 308 male broiler chickens were randomly allocated into eight treatments with four replicates each. A 2×2×2 factorial arrangement was applied, with two levels of coenzyme Q10 (0 and 40 mg/kg), two levels of L-carnitine (0 and 200 mg/kg) and two levels of ractopamine (0 and 10 mg/kg). The birds were reared until day 42 of age under standard conditions. Blood samples were collected at the end of grower and finisher periods from the wing vein. Four birds per group were sacrificed at day 42 of age. Except for carcass yield, other carcass traits were not significantly affected (p>0.05) by different levels of coenzyme Q10, L-carnitine, or ractopamine. Immune response parameters were significantly (p 0.05) different between the treatments. The lowest antibody titers against Newcastle disease virus and relative spleen weight were observed in control group. The results of this study suggest that addition of coenzyme Q10 and L-carnitine to broiler diets has benefit effect on immune response of broiler chickens.(AU)
Assuntos
Animais , Imunidade/fisiologia , Dieta/veterinária , Coenzimas/análise , Coenzimas/fisiologia , Carnitina/análise , /análise , Fenômenos Fisiológicos da Nutrição Animal/fisiologia , Ração Animal/análise , Programas de Nutrição , Coleta de Amostras Sanguíneas/veterinária , Testes Hematológicos/veterinária , Doença de NewcastleRESUMO
This study was conducted to evaluate the effects of coenzyme Q10, L-carnitine and ractopamine supplementation, alone and in combinations, on carcass traits and immune response of broiler chickens. Five hundred and twelve one-day-old Ross 308 male broiler chickens were randomly allocated into eight treatments with four replicates each. A 2×2×2 factorial arrangement was applied, with two levels of coenzyme Q10 (0 and 40 mg/kg), two levels of L-carnitine (0 and 200 mg/kg) and two levels of ractopamine (0 and 10 mg/kg). The birds were reared until day 42 of age under standard conditions. Blood samples were collected at the end of grower and finisher periods from the wing vein. Four birds per group were sacrificed at day 42 of age. Except for carcass yield, other carcass traits were not significantly affected (p>0.05) by different levels of coenzyme Q10, L-carnitine, or ractopamine. Immune response parameters were significantly (p 0.05) different between the treatments. The lowest antibody titers against Newcastle disease virus and relative spleen weight were observed in control group. The results of this study suggest that addition of coenzyme Q10 and L-carnitine to broiler diets has benefit effect on immune response of broiler chickens.
Assuntos
Animais , Carnitina/análise , Coenzimas/análise , Coenzimas/fisiologia , Dieta/veterinária , Fenômenos Fisiológicos da Nutrição Animal/fisiologia , Imunidade/fisiologia , Coleta de Amostras Sanguíneas/veterinária , Doença de Newcastle , Programas de Nutrição , Ração Animal/análise , Testes Hematológicos/veterináriaRESUMO
Inorganic pyrophosphatases are divided in two families, which differ both in structure and mechanism. All of them incorporate in its structure divalent metal cations. In 2003, it was reported for the first time that Rhodobacter capsulatus cytoplasmic pyrophosphatase belongs to family II. It is expected then, that this enzyme contains metal elements in its structure; however, this characterization has not been carried out yet. A fine application of accelerators is the use of proton beams to induce X-ray emission (PIXE) for analyzing the composition of biological macromolecules. The purpose of this work is to complement R. capsulatus cytoplasmic pyrophosphatase characterization by determining the presence of metal elements in its structure. Three different strategies were used: PAGE-PIXE, PAGE-Digestion-PIXE, and Dialysis-PIXE and when metals were found the metal/enzyme ratio was calculated. Only cobalt was found to be associated to the enzyme chemical structure in a ratio 3 Co/enzyme.
Assuntos
Metais/análise , Pirofosfatases/química , Rhodobacter capsulatus/enzimologia , Coenzimas/análise , Espectrometria por Raios X/métodosRESUMO
A Doença de Alzheimer é uma doença neurodegenerativa progressiva e de início tardio, que compromete principalmente as areas da cognição, julgamento e estabilidade emocional. Esta doença se caracteriza por dois tipos de lesões cerebrais características: emaranhados neurofibrilares e placas senis. Os emaranhados neurofibrilares são compostos por uma proteína do citoesqueleto (proteína tau) hiperfosforilada e agregada. As placas senis são formadas por agregados da proteína beta-amilóide. A doença de. Alzheimer é resultado da interação de vários fatores ainda incompletamente elucidados; não obstante, o estresse oxidativo e os processos inflamatórios ocupam posição de destaque dentre esses fatores. Neste trabalho, avaliamos as atividades das enzimas eritrocitárias superóxido dismutase, catalase e glutationa peroxidase, assim como o conteúdo plasmático de glutationa total, vitamina C, alfa-tocoferol, beta-caroteno, licopeno e coenzima Q IND 10. A esses parâmetros antioxidantes foram contrapostas medidas de oxidação de lipídios e proteínas plasmáticas. Adicionalmente, efetuamos a avaliação das expressões monocitárias de HLA-DR e CD-11b, e das citocinas IL-6, IL-1alfa e TNF-alfa. Nossos resultados mostram que os pacientes de .doença de Alzheimer possuem níveis circulantes de atocoferol inferiores aos pacientes controles, e possuem monócitos que apresentam maior expressão basal de HLA-DR e maior produção de IL-1alfa quando estimulados por LPS: Esses resultados fortalecem a hipótese inflamatória na doença de Alzheimer, de acordo com trabalhos recentes que apontam bons resultados com o alfa-tocoferol na sua prevenção e tratamento
Assuntos
Humanos , Idoso de 80 Anos ou mais , Ácido Ascórbico/análise , Catalase/análise , Doença de Alzheimer/prevenção & controle , Glutationa Peroxidase/análise , Superóxido Dismutase/análise , alfa-Tocoferol/análise , Proteínas Sanguíneas , Coenzimas/análise , Lipídeos , Monócitos , Oxidação , Transtornos de Deficit da Atenção e do Comportamento Disruptivo , beta Caroteno/análiseRESUMO
Isolate 1051 of Trichoderma harzianum, a mycoparasitic fungus, was found to impair development of the phytopathogen, Crinipellis perniciosa, in the field. This Trichoderma strain growing in liquid medium containing chitin produced substantial amounts of chitinases. The N-acetylglucosaminidase present in the culture-supernatant was purified to homogeneity by gel filtration and hydrophobic interaction chromatography, as demonstrated by SDS-PAGE analysis. The enzyme had a molecular mass of 36 kDa and hydrolyzed the synthetic substrate rho-nitrophenyl-N-acetylglucosaminide (rhoNGlcNAc) with Michaelis-Menten kinetics. Maximal activities were determined at pH 4.0 and a temperature range of 50-60 degrees C. Km and Vmax values for rhoNGlcNAc hydrolysis were 8.06 micromoles ml(-1) and 3.36 micromoles ml(-1) min(-1), respectively, at pH 6.0 and 37 degrees C. The enzyme was very sensitive to Fe3+, Mn2+ and Co2+ ions, but less sensitive to Zn2+, Al3+, Cu2+ and Ca2+. Glucose at a final concentration of 1 mM inhibited 65% of the original activity of the purified enzyme. Determination of the product (reducing sugar) of hydrolysis of C. perniciosa mycelium and scanning electron microscopic analysis revealed that the N-acetylglucosaminidase hydrolyses the C. perniciosa cell wall.