RESUMO
OBJECTIVE: The aim of this study was to investigate the effects of PRP on SAOS-2 cells in terms of cytokine expression, cell activity and oxidative stress. DESIGN: Cell line SAOS-2 (1×10(5)cells/mL) were grown in culture medium α-MEM with 10% FBS for 24h and stimulated (or not) with PRP at concentrations of 3, 10 and 20%, LPS (E. coli, 10g/mL) and IL-1ß (1mg/mL) for 24h. The supernatant was collected and analyzed for the expression of cytokines in a panel array, ALP using a commercial kit and NO(2)(-) with Griess reaction method. Also, the cells were analyzed using Western blot for RANKL and slot blotting for nitrotyrosine expression. RESULT: There were no significant differences amongst the groups in terms of NO(2)(-), protein nitrotyrosine content and RANKL expression. However, all stimuli increased ALP activity and in case of PRP, it was in a dose-dependent manner (p<0.001). Also, all stimuli induced an increase in cytokines and chemokines expression, but only PRP promoted an increase of component C5, sICAM-1 and RANTES expression. Whilst IL-1 receptor antagonist (IL-1ra) expression was down-regulated by PRP, both LPS and IL-1ß caused up-regulation of this cytokine. CONCLUSIONS: PRP can stimulate osteoblast activity and cytokine/chemokine release, as well as indicate some of the mediators that can (and cannot) be involved in this activation.
Assuntos
Fosfatase Alcalina/análise , Citocinas/análise , Osteoblastos/metabolismo , Plasma Rico em Plaquetas/fisiologia , Linhagem Celular Tumoral , Quimiocina CCL5/análise , Quimiocina CXCL1/análise , Complemento C5/análise , Relação Dose-Resposta a Droga , Escherichia coli , Fator Estimulador de Colônias de Granulócitos e Macrófagos/análise , Humanos , Molécula 1 de Adesão Intercelular/análise , Proteína Antagonista do Receptor de Interleucina 1/análise , Interleucina-1beta/farmacologia , Interleucinas/análise , Lipopolissacarídeos/farmacologia , Óxido Nítrico/análise , Osteoblastos/efeitos dos fármacos , Estresse Oxidativo/fisiologia , Ligante RANK/análise , Tirosina/análogos & derivados , Tirosina/análiseRESUMO
We investigated immunologic mechanisms and the role of complement in the pathogenesis of Argentine hemorrhagic fever, a disease caused by the Junin virus, a member of the arenavirus group. Total serum complement activity was reduced to 68 per cent of control values in patients with severe or moderate disease (P less than 0.001). C2, C3 and C5 values were also low (12 to 60 per cent) during the early acute period of the disease. However, serum C4 content was increased to 160 per cent of the control values in the same patients. Total complement activity returned to normal with clinical and laboratory recovery, at the time of detection of antibodies against Junin virus. C1q reactive material was found in four of 19 cases and no relation to the evolution of the disease could be established. These results suggest that immune complexes are not important in the pathogenesis of Argentine hemorrhagic fever, but that activation of the complement system has a role.
Assuntos
Anticorpos Antivirais/análise , Arenaviridae/imunologia , Arenavirus do Novo Mundo/imunologia , Proteínas do Sistema Complemento/análise , Febre Hemorrágica Americana/imunologia , Complemento C2/análise , Complemento C3/análise , Complemento C4/análise , Complemento C5/análise , Convalescença , Humanos , Fatores de TempoRESUMO
The purpose of this study was to examine normal serum values for the complement components, C3, C4, and C5, and total hemolytic complement (CH50) activity in 163 healthy infants and children, in the age range from birth through 14 years. There were statistically significant relationships of C3, C4, and C5, but not CH50 with age. None of the complement components or CH50 could be differentiated by sex or race. Tolerance limits for high and low values were projected for each complement measurement for 75%, 90%, and 95% of the general population. Our data confirm the differences in complement levels between children and adults and demonstrate a wide range of values within each group, reflecting the biologic variability of complement measurements. These results emphasize the importance of establishing normal pediatric values in any laboratory that measures complement profiles in various diseases of childhood.
Assuntos
Proteínas do Sistema Complemento/análise , Adolescente , População Negra , Criança , Pré-Escolar , Complemento C3/análise , Complemento C4/análise , Complemento C5/análise , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , População BrancaAssuntos
Proteínas Opsonizantes/sangue , Esplenectomia , Streptococcus pneumoniae/imunologia , Adolescente , Adulto , Criança , Pré-Escolar , Complemento C3/análise , Complemento C5/análise , História do Século XVIII , Doença de Hodgkin/cirurgia , Humanos , Hipertensão Portal/cirurgia , Imunodifusão , Lactente , Fagocitose , Infecções Pneumocócicas/imunologia , Properdina/análise , Púrpura Trombocitopênica/cirurgia , Baço/anormalidades , Baço/fisiologia , Talassemia/cirurgia , Ferimentos e Lesões/cirurgiaRESUMO
Previous reports have suggested that a defect in serum complement may contribute to the increased susceptibility to infection shown by patients with sickle cell anaemia (SCA). In order to define the nature of any complement abnormality in SCA, we investigated the complement system in eighty-seven patients during asymptomatic periods, and analysed factor B turnover in a small sample. In these patients geometric mean serum concentrations of functionally active factor B and factor D, and of C3 and C4 protein (expressed as a percentage of normal reference serum) were lower than in controls (78 percent vs. 107 percent, P<0.001, 86 percent vs. 103 percent, P<0.001, 91 percent vs. 100 percent, P<0.01, 89 percent vs. 105 percent, P<0.05 respectively). The ratio of the serum concentration of functionally active factor B to factor B protein was lower in patients than in controls (mean 75 percent s.d 16 percent vs. mean 93 percent, s.d 22 percent P<0.001), indicating a functional deficiency of factor B protein. In addition, the fractional catabolic rate of radiolabelled factor B was markedly increased in four out of seven asymptomatic patients studied, and was inversely related to the functional factor B concentration in serum (r=-0.59, P<0.05); factor B synthesis was uniformly increased. Complement activation was not related to the presence of circulating Clq binding material. We conclude that complement activation, rather than defective synthesis as previously suggested, contributes to the abnormalities in complement component concentration and function in asymptomatic subjects with sickle cell anaemia (Summary)
Assuntos
Humanos , Pré-Escolar , Criança , Adolescente , Adulto , Pessoa de Meia-Idade , Masculino , Feminino , Anemia Falciforme/imunologia , Proteínas do Sistema Complemento/metabolismo , Ativação do Complemento , Complemento C1/análise , Complemento C3/metabolismo , Complemento C4/análise , Complemento C5/análise , Fator B do Complemento/biossíntese , Fator B do Complemento/metabolismo , Fator D do Complemento/metabolismoRESUMO
Of 182 patients with acute glomerulonephritis, 20 had normal C3 levels at onset. Normocomplementemic and hypocomplementemic patients were similar with respect to incidence and site of preceding streptococcal infection, elevation of ASO titer, distribution by age, sex, race, season, and year,\and glomerular morphology by light and electron microscopy. They differed in that the normocomplementemic patients tended to have normal serum C5 levels and, for reasons not clear, reduced serum albumin and elevated cholesterol levels. The consistent absence by immunofluorescence of IgG in the glomeruli of five hypocomplementemic patients and its presence in five normocomplementemic patients was considered a chance observation. The data suggest that in each group the nephritis was poststreptococcal and that the mechanism producing poststreptococcal glomerulonephritis is capable of acting independently of that activating circulating C3.