RESUMO
The objective of this study is to evaluate the effects of in-egg inoculation with sources of conjugated linoleic acid (CLA) and lauric acid (AL), going by the weight of lymphoid organs and the biochemical profile of the blood of cut quails, from one to 35 days of age. We used 360 quails distributed in a completely randomized design, in six treatments and six replicates of 10 birds per plot. Corn oil (OM) was used to dilute the CLA and AL. The experimental treatments were: healthy eggs (control); eggs inoculated with OM thinner; eggs inoculated with CLA 120 mg / 50 mL OM; eggs inoculated with CLA 240 mg / 50 mL OM; eggs inoculated with AL 60 mg / 50 mL OM and eggs inoculated with AL 90 mg / 50 mL OM. The results were analyzed through Program R at the level of 5% significance. To verify the normal distribution of errors, the Shapiro Wilk normality test was applied. At 21 days, an effect of p < 0.05 was observed for the total blood cholesterol content, inoculations with CLA reduced, even as AL increased, regardless of the level. At 35 days, it was found (p < 0.05) that the levels of total cholesterol and AST in the blood decreased with inoculations of CLA and AL at the lower levels, compared to those that did not receive the inoculations. The CLA and AL levels supplemented via egg inoculations did not interfere with the weight of the lymphoid organs (thymus, Fabricius bursa) of the birds, at both ages. Supplementation in eggs of cut quails with CLA 240 mg reduced the total blood cholesterol content compared to CLA 120 mg, at 21 days of age. At 35 days of age, higher concentrations of CLA and AL increased the total cholesterol and AST in the blood of birds and AL had a greater effect on the increase than CLA.
O objetivo deste trabalho foi avaliar os efeitos da inoculação in ovo com fontes de ácido linoleico conjugado (CLA) e ácido láurico (AL), sobre os órgãos linfoides e o perfil bioquímico do sangue de codornas de corte de um a 35 dias de idade. Foram utilizadas 360 codornas distribuídas em delineamento inteiramente casualizado, em seis tratamentos e seis repetições de 10 aves por parcela. Os tratamentos experimentais foram: ovos íntegros (controle); ovos inoculados com diluidor OM; ovos inoculados com CLA 120 mg/50mL OM; ovos inoculados com CLA 240 mg/50mL OM; ovos inoculados com AL 60 mg/50mL OM e ovos inoculados com AL 90 mg/50mL OM. Aos 21 dias observou-se efeito (P<0,05) para teor de colesterol total no sangue, as inoculações com CLA reduzem, enquanto o AL aumenta independentemente do nível. Os resultados foram analisados pelo programa R no nível de significância de 5%. Para verificar a distribuição normal dos erros, foi aplicado o teste de normalidade Shapiro-Wilk. Aos 21 dias, observou-se efeito (P < 0,05) para o teor total de colesterol no sangue, inoculações com CLA reduzida, enquanto a AL aumenta independentemente do nível. Aos 35 dias, verificou-se (P < 0,05) que os níveis de colesterol total e AST no sangue diminuíram com as inoculações de CLA e AL em níveis mais baixos, comparados àqueles que não receberam inoculações. Os níveis de CLA e AL suplementados por inoculações de ovos não interferiram no peso dos órgãos linfoides (timo, bursa de Fabricius) de aves em ambas as idades. A suplementação em ovos de codornas de corte com CLA 240 mg reduz o conteúdo total de colesterol no sangue em comparação com o CLA 120 mg aos 21 dias de idade. Aos 35 dias de idade, concentrações mais altas de CLA e AL aumentam o colesterol total e AST no sangue das aves e o AL tem um efeito maior em aumentar o CLA sanguíneo.
Assuntos
Animais , Coturnix/imunologia , Coturnix/sangue , Ácido Linoleico/administração & dosagemRESUMO
The objective of this study is to evaluate the effects of in-egg inoculation with sources of conjugated linoleic acid (CLA) and lauric acid (AL), going by the weight of lymphoid organs and the biochemical profile of the blood of cut quails, from one to 35 days of age. We used 360 quails distributed in a completely randomized design, in six treatments and six replicates of 10 birds per plot. Corn oil (OM) was used to dilute the CLA and AL. The experimental treatments were: healthy eggs (control); eggs inoculated with OM thinner; eggs inoculated with CLA 120 mg / 50 mL OM; eggs inoculated with CLA 240 mg / 50 mL OM; eggs inoculated with AL 60 mg / 50 mL OM and eggs inoculated with AL 90 mg / 50 mL OM. The results were analyzed through Program R at the level of 5% significance. To verify the normal distribution of errors, the Shapiro Wilk normality test was applied. At 21 days, an effect of p < 0.05 was observed for the total blood cholesterol content, inoculations with CLA reduced, even as AL increased, regardless of the level. At 35 days, it was found (p < 0.05) that the levels of total cholesterol and AST in the blood decreased with inoculations of CLA and AL at the lower levels, compared to those that did not receive the inoculations. The CLA and AL levels supplemented via egg inoculations did not interfere with the weight of the lymphoid organs (thymus, Fabricius bursa) of the birds, at both ages. Supplementation in eggs of cut quails with CLA 240 mg reduced the total blood cholesterol content compared to CLA 120 mg, at 21 days of age. At 35 days of age, higher concentrations of CLA and AL increased the total cholesterol and AST in the blood of birds and AL had a greater effect on the increase than CLA.(AU)
O objetivo deste trabalho foi avaliar os efeitos da inoculação in ovo com fontes de ácido linoleico conjugado (CLA) e ácido láurico (AL), sobre os órgãos linfoides e o perfil bioquímico do sangue de codornas de corte de um a 35 dias de idade. Foram utilizadas 360 codornas distribuídas em delineamento inteiramente casualizado, em seis tratamentos e seis repetições de 10 aves por parcela. Os tratamentos experimentais foram: ovos íntegros (controle); ovos inoculados com diluidor OM; ovos inoculados com CLA 120 mg/50mL OM; ovos inoculados com CLA 240 mg/50mL OM; ovos inoculados com AL 60 mg/50mL OM e ovos inoculados com AL 90 mg/50mL OM. Aos 21 dias observou-se efeito (P<0,05) para teor de colesterol total no sangue, as inoculações com CLA reduzem, enquanto o AL aumenta independentemente do nível. Os resultados foram analisados pelo programa R no nível de significância de 5%. Para verificar a distribuição normal dos erros, foi aplicado o teste de normalidade Shapiro-Wilk. Aos 21 dias, observou-se efeito (P < 0,05) para o teor total de colesterol no sangue, inoculações com CLA reduzida, enquanto a AL aumenta independentemente do nível. Aos 35 dias, verificou-se (P < 0,05) que os níveis de colesterol total e AST no sangue diminuíram com as inoculações de CLA e AL em níveis mais baixos, comparados àqueles que não receberam inoculações. Os níveis de CLA e AL suplementados por inoculações de ovos não interferiram no peso dos órgãos linfoides (timo, bursa de Fabricius) de aves em ambas as idades. A suplementação em ovos de codornas de corte com CLA 240 mg reduz o conteúdo total de colesterol no sangue em comparação com o CLA 120 mg aos 21 dias de idade. Aos 35 dias de idade, concentrações mais altas de CLA e AL aumentam o colesterol total e AST no sangue das aves e o AL tem um efeito maior em aumentar o CLA sanguíneo.(AU)
Assuntos
Animais , Coturnix/sangue , Coturnix/imunologia , Ácido Linoleico/administração & dosagemRESUMO
Environmental challenges are integrated in the inmunoneuroendocrine interplay, impacting the immune system of the challenged individuals, and potentially implying transgenerational effects on their offspring. This study addressed whether dietary supplementation with thymol can modulate the immune response of adult Japanese quail when simultaneously exposed to an inoculum of inactivated Salmonella Enteritidis and a chronic heat stress (CHS). We also evaluated whether the experienced situations by adults can affect the immune response of their undisturbed offspring. In the parental generation, supplemented quail exposed to CHS had a higher inflammatory response and similar values of the heterophil/lymphocyte (H/L) ratio than those that were not supplemented. In their offspring, those chicks whose parents were exposed to CHS showed higher inflammatory response and lower antibody production. Regarding the H/L ratio, chicks whose parents were supplemented showed lower H/L ratio values. Dietary supplementation with thymol partially and positively modulated the inflammatory response and avoided H/L ratio alteration in the parental generation exposed to high environmental temperatures, suggesting these adults were better at dealing with the challenge. The lower H/L ratio values in the offspring suggests that chicks are more capable to deal with potential stressful situations associated with conventional breeding conditions.
Assuntos
Ração Animal , Doenças das Aves/prevenção & controle , Coturnix/imunologia , Transtornos de Estresse por Calor/veterinária , Salmonella enteritidis/imunologia , Timol/administração & dosagem , Animais , Doenças das Aves/sangue , Doenças das Aves/imunologia , Doenças das Aves/microbiologia , Coturnix/microbiologia , Feminino , Transtornos de Estresse por Calor/sangue , Transtornos de Estresse por Calor/imunologia , Transtornos de Estresse por Calor/prevenção & controle , Temperatura Alta/efeitos adversos , Contagem de Linfócitos , Linfócitos/imunologia , Masculino , Exposição Materna , Neuroimunomodulação/efeitos dos fármacos , Óvulo/imunologia , Exposição Paterna , Fatores SexuaisRESUMO
Coturniculture has increased significantly in the last decades. There are several pathogens that can affect these birds. Among the diseases, fowl typhoid stands out as a disease with a potentially great impact to the poultry industry. The objective of this the study was to evaluate the effect of doses and administration routes of live 9R vaccine on protection of Japanese quails against experimental infection with Salmonella Gallinarum (SG). Two hundred and fifty birds were used, divided into five groups: G1, oral vaccination with one dose; G2, oral vaccination with 2 doses; G3, subcutaneous vaccination with one dose; G4, subcutaneous vaccination with two doses and G5 not vaccinated. All birds from all five groups were challenged with SG at an age of 45 days. SG was quantified in the periods of one, four, seven and twelve days after the challenge. The presence of clinical signs and macroscopic lesions of the disease were observed. The groups vaccinated by subcutaneous route had a higher egg production and lower mortality rate. Birds receiving a dose of the vaccine by subcutaneous route also showed lower amount of SG in the liver and spleen seven days after the challenge.(AU)
A coturnicultura tem aumentado significativamente nas últimas décadas. Existem vários patógenos que podem afetar essas aves. Entre as doenças, o tifo aviário se destaca como uma doença de grande impacto para a indústria avícola. O objetivo deste estudo foi avaliar o efeito de doses e vias de administração da vacina viva 9R na proteção de codornas japonesas contra infecção experimental por Salmonella Gallinarum (SG). Foram utilizadas duzentos e cinquenta aves, divididas em cinco grupos: G1, vacinação oral com uma dose; G2, vacinação oral com 2 doses; G3, vacinação subcutânea com uma dose; G4, vacinação subcutânea com duas doses e G5 não vacinado. Todas as aves dos cinco grupos foram desafiadas com SG aos 45 dias de idade. A SG foi quantificada nos períodos de um, quatro, sete e doze dias após o desafio. Foi observada a presença de sinais clínicos e lesões macroscópicas da doença. Os grupos vacinados por via subcutânea apresentaram maior produção de ovos e menor taxa de mortalidade. Aves recebendo uma dose da vacina por via subcutânea também apresentaram menor quantidade de SG no fígado e baço sete dias após o desafio.(AU)
Assuntos
Animais , Salmonella/imunologia , Vacinas/administração & dosagem , Vias de Administração de Medicamentos/veterinária , Coturnix/imunologiaRESUMO
Coturniculture has increased significantly in the last decades. There are several pathogens that can affect these birds. Among the diseases, fowl typhoid stands out as a disease with a potentially great impact to the poultry industry. The objective of this the study was to evaluate the effect of doses and administration routes of live 9R vaccine on protection of Japanese quails against experimental infection with Salmonella Gallinarum (SG). Two hundred and fifty birds were used, divided into five groups: G1, oral vaccination with one dose; G2, oral vaccination with 2 doses; G3, subcutaneous vaccination with one dose; G4, subcutaneous vaccination with two doses and G5 not vaccinated. All birds from all five groups were challenged with SG at an age of 45 days. SG was quantified in the periods of one, four, seven and twelve days after the challenge. The presence of clinical signs and macroscopic lesions of the disease were observed. The groups vaccinated by subcutaneous route had a higher egg production and lower mortality rate. Birds receiving a dose of the vaccine by subcutaneous route also showed lower amount of SG in the liver and spleen seven days after the challenge.(AU)
A coturnicultura tem aumentado significativamente nas últimas décadas. Existem vários patógenos que podem afetar essas aves. Entre as doenças, o tifo aviário se destaca como uma doença de grande impacto para a indústria avícola. O objetivo deste estudo foi avaliar o efeito de doses e vias de administração da vacina viva 9R na proteção de codornas japonesas contra infecção experimental por Salmonella Gallinarum (SG). Foram utilizadas duzentos e cinquenta aves, divididas em cinco grupos: G1, vacinação oral com uma dose; G2, vacinação oral com 2 doses; G3, vacinação subcutânea com uma dose; G4, vacinação subcutânea com duas doses e G5 não vacinado. Todas as aves dos cinco grupos foram desafiadas com SG aos 45 dias de idade. A SG foi quantificada nos períodos de um, quatro, sete e doze dias após o desafio. Foi observada a presença de sinais clínicos e lesões macroscópicas da doença. Os grupos vacinados por via subcutânea apresentaram maior produção de ovos e menor taxa de mortalidade. Aves recebendo uma dose da vacina por via subcutânea também apresentaram menor quantidade de SG no fígado e baço sete dias após o desafio.(AU)
Assuntos
Animais , Salmonella/imunologia , Vacinas/administração & dosagem , Vias de Administração de Medicamentos/veterinária , Coturnix/imunologiaRESUMO
ABSTRACT The objective of the present study was to evaluate the probiotic effects of different concentrations of four selected native Lactobacillus strains on the growth performance and serum biochemical parameters of Japanese quails. A completely randomized design (CRD) was applied, including seven probiotic treatments with four replicates of 20 quails each, totaling 560 quails. Treatments were applied for five weeks. Four native Lactobacillus strains were anaerobically grown in a 10-L batch fermenter and lyophilized (1010 CFU/g). Treatments were as follows: T1: control (basal diet); T2: commercial probiotic CP1; T3: commercial probiotic CP2; and T4, T5, T6, and T7: four native strains added at levels of 50, 100, 150, and 200 g/ton diet, respectively. The native probiotics significantly improved body weight gain (BWG) and feed conversion ratio (FCR) during the starter, finisher, and overall periods (35 days) (p 0.05), whereas no significant effect was observed on feed intake. The native strains significantly influenced the serum glucose, total protein, globulin, phosphorus, uric acid, low-density lipoprotein (LDL), LDL/HDL ratio, white blood cell counts (WBC), and mean corpuscular hemoglobin concentration (MCHC) of quails during the rearing period (p 0.05), whereas treatments had no influence (p>0.05) on blood cholesterol, calcium, high-density lipoprotein (HDL), or hemoglobin (HB) levels or on red blood cell counts (RBC). The cecal and small intestine samples of the quails fed the native Lactobacillus strains contained significantly higher Lactobacillus spp. and lower E. coli populations compared with the control diet and those supplemented with commercial probiotics. It was concluded that the use of the native Lactobacillus strains (150 g/ton diet) promoted the best performance of Japanese quails.
Assuntos
Animais , Biomarcadores/análise , Biomarcadores/metabolismo , Coturnix/crescimento & desenvolvimento , Coturnix/imunologia , Coturnix/metabolismo , Probióticos , Lactobacillus/citologia , Lactobacillus/químicaRESUMO
ABSTRACT The objective of the present study was to evaluate the probiotic effects of different concentrations of four selected native Lactobacillus strains on the growth performance and serum biochemical parameters of Japanese quails. A completely randomized design (CRD) was applied, including seven probiotic treatments with four replicates of 20 quails each, totaling 560 quails. Treatments were applied for five weeks. Four native Lactobacillus strains were anaerobically grown in a 10-L batch fermenter and lyophilized (1010 CFU/g). Treatments were as follows: T1: control (basal diet); T2: commercial probiotic CP1; T3: commercial probiotic CP2; and T4, T5, T6, and T7: four native strains added at levels of 50, 100, 150, and 200 g/ton diet, respectively. The native probiotics significantly improved body weight gain (BWG) and feed conversion ratio (FCR) during the starter, finisher, and overall periods (35 days) (p 0.05), whereas no significant effect was observed on feed intake. The native strains significantly influenced the serum glucose, total protein, globulin, phosphorus, uric acid, low-density lipoprotein (LDL), LDL/HDL ratio, white blood cell counts (WBC), and mean corpuscular hemoglobin concentration (MCHC) of quails during the rearing period (p 0.05), whereas treatments had no influence (p>0.05) on blood cholesterol, calcium, high-density lipoprotein (HDL), or hemoglobin (HB) levels or on red blood cell counts (RBC). The cecal and small intestine samples of the quails fed the native Lactobacillus strains contained significantly higher Lactobacillus spp. and lower E. coli populations compared with the control diet and those supplemented with commercial probiotics. It was concluded that the use of the native Lactobacillus strains (150 g/ton diet) promoted the best performance of Japanese quails.(AU)
Assuntos
Animais , Coturnix/crescimento & desenvolvimento , Coturnix/imunologia , Coturnix/metabolismo , Biomarcadores/análise , Biomarcadores/metabolismo , Probióticos , Lactobacillus/química , Lactobacillus/citologiaRESUMO
Stress is the loss of homeostasis by external forces or stressors. Manipulation, transport, contamination, and other procedures involved in production could be considered stressors. Contamination is a problem commonly faced by producers in the poultry industry. Aflatoxicosis is one of the most common infections resulting from feed contaminated with Aspergillus flavus and Aspergillus parasiticus. This study evaluated the potential effects of the combined administration of aflatoxin B(1) (AFB(1)) and corticosterone on biochemical (concentration of globulins, proteins, and albumin) and immunological (inflammatory response and heterophil:lymphocyte ratio) parameters of Japanese quail. Potential sex effects on those parameters were also considered. The provision of corticosterone in drinking water is a method used for mimicking the effects of chronic stress in avian species. At 35 d of age, 24 mixed-sex groups of 4 animals (2 males and 2 females) were housed in cages and assigned to 1 of 4 treatments: plain drinking water and laying diet, corticosterone administration in drinking water, feed contamination with AFB(1) (100 µg/kg of feed), or corticosterone plus AFB(1) administration. There were 6 cages per treatment. No significant effect of sex in any of the parameters analyzed was detected. Hypoproteinemia, hypoalbuminemia, and hypoglobulinemia were observed in animals treated with corticosterone or contaminated feed. These responses were exacerbated when the factors were combined. The immunodepressive effect of corticosterone administration was confirmed, and a higher effect was noticed when combined with the aflatoxin contamination. Aflatoxin contamination affected birds' physiology similar to a chronic stressor stimulation because it elevates the heterophil:lymphocyte ratio. This study suggests that the effects of the AFB(1) contamination are further increased when overlapped with a chronic stressful stimulation and emphasizes the importance of controlling potential stressor combinations during animal rearing to preserve not only the animal's health status but also their welfare.
Assuntos
Ração Animal/microbiologia , Coturnix/fisiologia , Contaminação de Alimentos , Aflatoxina B1/administração & dosagem , Aflatoxina B1/análise , Criação de Animais Domésticos , Animais , Análise Química do Sangue/veterinária , Corticosterona/administração & dosagem , Corticosterona/análise , Coturnix/sangue , Coturnix/imunologia , Água Potável , Feminino , Masculino , Distribuição Aleatória , Caracteres Sexuais , Estresse FisiológicoRESUMO
Procedures in the commercial production of animals involve stressful situations which lessen the animal's welfare. This study on Japanese quail evaluated whether an environmental enrichment manipulation can affect avian immune responses and if combined with a chronic stressor exposure can help to counteract the negative effects of stress on the immune system. Potential gender effects were also considered. After hatch, half of the birds were housed in non-enriched boxes and half were housed in environmentally enriched boxes. From day 33 to 42 of age, all birds within half of the non-enriched and enriched boxes remained undisturbed while the other half were daily exposed to a 15 min restraint stressor (chronic stressor). The inflammatory response (lymphoproliferation after phytohemagglutinin-p), percentage of lymphocytes, heterophil/lymphocyte (H/L) ratio and primary antibody response against sheep red blood cells were assessed. The chronic stressor application and the enrichment procedure, respectively, either increased or reduced the four immunological parameters evaluated and always in opposite directions. Males consistently showed lower antibody titres than females and presented the highest H/L ratio in response to the stressor when reared in the non-enriched environment. The findings indicate that submitting these animals to an enriched environment can be effectively used to improve their immune response and to reduce the detrimental effects of a stressor exposure.
Assuntos
Coturnix/imunologia , Meio Ambiente , Estresse Psicológico/imunologia , Criação de Animais Domésticos , Animais , Eritrócitos/imunologia , Feminino , Abrigo para Animais , Sistema Imunitário/imunologia , Imunidade Humoral/imunologia , Contagem de Linfócitos , Masculino , Restrição Física/fisiologia , Ovinos/imunologiaRESUMO
The aim was to evaluate the excretion of residues of aflatoxin B(1) (AFB(1)), aflatoxin M(1) (AFM(1)), aflatoxin B(2a) (AFB(2a)) and aflatoxicol (AFL) in eggs of laying Japanese quail fed rations with low levels of aflatoxin B(1) for 90 days. The quail were randomly assigned into four experimental groups and given prepared rations containing either 0 (controls), 25, 50 or 100 microg AFB(1) kg(-1) feed. Thirty-two eggs per treatment were collected on days 1-7, 10, 20, 30, 60 and 90 of the aflatoxin treatment period, and submitted to aflatoxin analysis by high-performance liquid chromatography. Average egg production and feed consumption were not affected ( p > 0.05) by AFB(1). Egg weight was significantly lower ( p<0.05) only for groups exposed to 100 microg AFB(1) kg(-1). Residues of aflatoxins were detected in eggs at levels that ranged from 0.01 to 0.08 microg kg(-1) (AFB(1)), 0.03-0.37 microg kg(-1) (AFM(1)), 0.01-1.03 microg kg(-1)(AFB(2a)) and 0.01-0.03 microg kg(-1) (AFL). Results indicate that the excretion of aflatoxin residues in quail eggs might occur at relatively low concentrations under conditions of long-term exposure of quail to low levels of AFB(1).