RESUMO
Recent studies have shown how intestinal parasites can modulate gut microbiota. This observation is not surprising since the human intestinal lumen, like any other niche, is a battlefield of microbial competition, and Eukaryotes can affect bacterial populations. Intestinal pathogenic protist has been associated with reshaping the microbial community structure; however, the interactions between the colonic bacterial communities and parasites like Blastocystis spp., Entamoeba coli, and Endolimax nana have been poorly studied. In this work, we studied the distal intestinal bacterial microbiota of 49 children attending 7 public daycare centers in Medellin, Colombia, and compared the bacterial microbiota structure in the presence or absence of the protists Blastocystis spp., E. coli, and E. nana. Parasite colonization was associated with an increase in bacterial richness. Moreover, Blastocystis spp. presented a positive relationship with Prevotella, since this bacterium was selectively enriched in children carrying it. Remarkably, the E. coli colonized children showed a microbial profile that was closer to uninfected controls, although some bacterial taxa displayed to be enriched. This is the case for Akkermansia, which showed to be favored in E. coli colonized individuals, while notably reduced in the Blastocystis spp. parasitized group.
Assuntos
Amebíase/microbiologia , Fezes/parasitologia , Microbioma Gastrointestinal/fisiologia , Bactérias/genética , Blastocystis/patogenicidade , Infecções por Blastocystis/microbiologia , Pré-Escolar , Colômbia , Endolimax/patogenicidade , Entamoeba/patogenicidade , Entamebíase/microbiologia , Fezes/microbiologia , Feminino , Microbioma Gastrointestinal/genética , Humanos , Lactente , Enteropatias Parasitárias/microbiologia , Enteropatias Parasitárias/parasitologia , Masculino , Prevotella/genéticaRESUMO
Formalin preserved fecal samples from 6,058 and 5,863 outpatients were examined for intestinal parasites during 1995 and 1996 respectively. Prevalence rates of infections by intestinal protozoa in both years were similar. By age group (0-9, 10-19 and >20 years old). Blastocystis hominis was observed in 18.6-19.3, 37.0-31.1 and 25.3-25.4 percent in 1995-1996 respectively. Prevalence of giardia intestinalis infections decreased from 16.6-17.4 per cent in the 0-9 year-old children group to 4.1-4.5 percent in patients over 20 years. Overall percentages of infection by entamoeba histolytica varied between 4.2 and 10.9.Rates of infections by G. intestinalis, E. histolytica, and entamoeba coli observed during rainy-cold months (april-september) of the year versus dry-warmy period (october-march) were the same. On the contrary, more cases of B. hominis infection 25.8 percent versus 18.2 percent (this difference being statistically significant, p >0.001) were observed during rainy-cold months of the year
Assuntos
Humanos , Masculino , Feminino , Lactente , Pré-Escolar , Adolescente , Adulto , Enteropatias Parasitárias/epidemiologia , Infecções por Protozoários , Distribuição por Idade , Blastocystis hominis/isolamento & purificação , Blastocystis hominis/patogenicidade , Endolimax/isolamento & purificação , Endolimax/patogenicidade , Entamoeba/isolamento & purificação , Entamoeba/patogenicidade , Fezes/parasitologia , Giardia lamblia/isolamento & purificação , Giardia lamblia/patogenicidade , Pacientes AmbulatoriaisRESUMO
Blastocystis hominis, um parasito intestinal do homem, foi anteriormente descrito como levedura. Ziertd e colaboradores estudaram e reclassificaram este organismo como um protozoário. O B. hominis no exame parasitológico das fezes deverá ser quantificado (raros, poucos, moderados, muitos). No mínimo três exames das fezes, para a pesquisa de ovos e parasitas (incluindo esfregaços permanentes corados), deveräo ser realizados, para assegurar a ausência de outros protozoários, antes de atribuir os sintomas ao B. hominis. A presença de outros agentes microbianos deverá também ser considerada