RESUMO
Interdigital foot infections are mostly caused initially by dermatophytes, yeasts and less frequently by bacteria. Erythrasma caused by Corynebacterium minutissimum can be confused with superficial mycoses. The aim of the study was to determine the prevalence of the etiologic agents of superficial mycoses and the frequency of Corynebacterium minutissimum in interdigital foot infections. All the samples obtained from the 121 patients with interdigital foot infections were examined directly with the use of 20% potassium hydroxide mounts and Gram stain under the microscope and cultured on Sabouraud's dextrose agar plates. In identification of superficial mycoses, the rate was found to be 14% with the cultural method and 14% with direct microscopic examination. Using a combination of direct microscopic examination and culture, a 33.8% ratio was achieved. In the culture of these samples, the most isolated factor was Trichophyton rubrum (33.7%). In 24 of the patients (19.8%) Corynebacterium minutissimum was detected by Gram staining, in 6 of these patients Trichophyton rubrum was found, Trichophyton mentagrophytes was found in 2 and Trichosporon spp. was found in 1. The examination of interdigital foot lesions in the laboratory, the coexistence of erythrasma with dermatophytes and yeast should be considered.
Assuntos
Arthrodermataceae/isolamento & purificação , Corynebacterium/isolamento & purificação , Dermatomicoses/epidemiologia , Eritrasma/epidemiologia , Doenças do Pé/epidemiologia , Dermatomicoses/microbiologia , Eritrasma/microbiologia , Doenças do Pé/microbiologia , Humanos , Técnicas Microbiológicas , PrevalênciaRESUMO
Interdigital foot infections are mostly caused initially by dermatophytes, yeasts and less frequently by bacteria. Erythrasma caused by Corynebacterium minutissimum can be confused with superficial mycoses. The aim of the study was to determine the prevalence of the etiologic agents of superficial mycoses and the frequency of Corynebacterium minutissimum in interdigital foot infections. All the samples obtained from the 121 patients with interdigital foot infections were examined directly with the use of 20% potassium hydroxide mounts and Gram stain under the microscope and cultured on Sabouraud's dextrose agar plates. In identification of superficial mycoses, the rate was found to be 14% with the cultural method and 14% with direct microscopic examination. Using a combination of direct microscopic examination and culture, a 33.8% ratio was achieved. In the culture of these samples, the most isolated factor was Trichophyton rubrum (33.7%). In 24 of the patients (19.8%) Corynebacterium minutissimum was detected by Gram staining, in 6 of these patients Trichophyton rubrum was found, Trichophyton mentagrophytes was found in 2 and Trichosporon spp. was found in 1. The examination of interdigital foot lesions in the laboratory, the coexistence of erythrasma with dermatophytes and yeast should be considered.
Assuntos
Humanos , Arthrodermataceae/isolamento & purificação , Corynebacterium/isolamento & purificação , Dermatomicoses/epidemiologia , Eritrasma/epidemiologia , Doenças do Pé/epidemiologia , Dermatomicoses/microbiologia , Eritrasma/microbiologia , Doenças do Pé/microbiologia , Técnicas Microbiológicas , PrevalênciaRESUMO
Interdigital foot infections are mostly caused initially by dermatophytes, yeasts and less frequently by bacteria. Erythrasma caused by Corynebacterium minutissimum can be confused with superficial mycoses. The aim of the study was to determine the prevalence of the etiologic agents of superficial mycoses and the frequency of Corynebacterium minutissimum in interdigital foot infections. All the samples obtained from the 121 patients with interdigital foot infections were examined directly with the use of 20% potassium hydroxide mounts and Gram stain under the microscope and cultured on Sabouraud's dextrose agar plates. In identification of superficial mycoses, the rate was found to be 14% with the cultural method and 14% with direct microscopic examination. Using a combination of direct microscopic examination and culture, a 33.8% ratio was achieved. In the culture of these samples, the most isolated factor was Trichophyton rubrum (33.7%). In 24 of the patients (19.8%) Corynebacterium minutissimum was detected by Gram staining, in 6 of these patients Trichophyton rubrum was found, Trichophyton mentagrophytes was found in 2 and Trichosporon spp. was found in 1. The examination of interdigital foot lesions in the laboratory, the coexistence of erythrasma with dermatophytes and yeast should be considered.
Assuntos
Humanos , Arthrodermataceae/isolamento & purificação , Corynebacterium/isolamento & purificação , Dermatomicoses/epidemiologia , Eritrasma/epidemiologia , Doenças do Pé/epidemiologia , Dermatomicoses/microbiologia , Eritrasma/microbiologia , Doenças do Pé/microbiologia , Técnicas Microbiológicas , PrevalênciaRESUMO
BACKGROUND: Erythrasma is a superficial infection caused by Corynebacterium minutissimum and affects the major skin folds and the interdigital regions of the feet. It is characterized by erythematous, brown, scaly patches and maceration, and exhibits coral-red fluorescence under Wood light. OBJECTIVE: The aim of this study was to determine the frequency of erythrasma in patients with interdigital lesions. METHODS: An open, prospective, longitudinal, observational study was performed in a hospital in Mexico City between March and December, 2006. All patients with interdigital lesions were examined with a Wood lamp and direct examination was performed with 20 % potassium hydroxide. Cultures were done in Sabouraud dextrose agar and brain heart infusion agar, and smears were analyzed. General characteristics and concomitant diseases were recorded. RESULTS: We examined 73 patients, of whom 24 (32.8 %) were diagnosed with erythrasma based on coral-red fluorescence under Wood light and identification of corynebacteria by Gram staining. The disease was more common in women (83.33 %) and the mean age of the patients was 43.5 years. The main clinical findings were scaling and maceration, and the fourth interdigital web was the most commonly affected. Corynebacterium could not be isolated in any of the cases. Mycology was positive in 15 cases (62.5 %) and the following microorganisms were isolated: Candida (16.6 %), dermatophytes (12.5 %), and Trichosporon (4.1 %). CONCLUSIONS: Interdigital erythrasma is a common condition and can be easily confused with interdigital tinea. It persists if not treated appropriately. Rapid diagnosis is easily obtained by examination with a Wood lamp, while culture is difficult and unnecessary for diagnosis. The coexistence of erythrasma with dermatophytes and Candida should be considered when the interdigital webs are affected.
Assuntos
Dermatomicoses/epidemiologia , Eritrasma/epidemiologia , Dermatoses do Pé/epidemiologia , Dermatoses da Mão/epidemiologia , Adolescente , Adulto , Idoso , Infecções por Corynebacterium/diagnóstico , Estudos Transversais , Dermatomicoses/diagnóstico , Dermatomicoses/microbiologia , Dermatomicoses/patologia , Diagnóstico Diferencial , Eritrasma/diagnóstico , Eritrasma/microbiologia , Eritrasma/patologia , Feminino , Fluorometria , Dermatoses do Pé/diagnóstico , Dermatoses do Pé/microbiologia , Dermatoses do Pé/patologia , Dermatoses da Mão/diagnóstico , Dermatoses da Mão/microbiologia , Dermatoses da Mão/patologia , Humanos , Estudos Longitudinais , Masculino , México/epidemiologia , Pessoa de Meia-Idade , Prevalência , Estudos Prospectivos , População UrbanaRESUMO
El eritrasma es una infección superficial de la piel que afecta la capa córnea y es causado por una bacteria grampositiva, Corynebacterium minutissimum. Se presenta como manchas eritematosas a marrón en grandes pliegues y como descamación y maceración en pliegues interdigitales; es asintomática, aunque en algunos casos se acompaña de prurito. El diagnóstico es clínico por luz de Wood que da una fluorescencia rojo coral de las áreas afectadas. Su curso es benigno, aunque persiste si no se da un tratamiento adecuado.
Erythrasma is a superficial skin infection of the stratum corneum caused by a gram-positive bacteria, Corynebacterium minutissimum. It is characterized by reddish-brown areas affecting occluded intertriginous zones such as axillae, inframammary folds, and as irregular scaly patches or macerated lesions on toeweb spaces. It is asymptomatic, though sometimes accompanied by pruritus. Clinical diagnosis is supported by a red fluorescence under Wood s light examination. It is benign, yet persistent if not adequately treated.
Assuntos
Humanos , Eritrasma/diagnóstico , Eritrasma/terapia , Diagnóstico Diferencial , Eritrasma/epidemiologia , Eritrasma/microbiologia , Infecções por Corynebacterium/complicações , PrognósticoRESUMO
Se comentan los datos históricos, microbiológicos, patógenos y epidemiológicos del eritrasma, así como sus características clínicas y de laboratorio y su tratamiento. El agente causal es Corynebacterium minutissimum y se menciona que el eritrasma es una enfermedad común en nuestro medio, aunque se desconoce su frecuencia real. Es posible que sea mal diagnosticada o poco reportada debido a su relativamente fácil diagnóstico o a su favorable reacción ante los diversos tratamientos existentes
Assuntos
Humanos , Infecções por Corynebacterium , Infecções por Corynebacterium/diagnóstico , Corynebacterium/patogenicidade , Eritrasma , Eritrasma/diagnóstico , Eritrasma/tratamento farmacológico , Eritrasma/história , Eritrasma/microbiologia , Raios UltravioletaRESUMO
The scales are collected by pressing small pieces of scotch tape (about 4 cm length and 2 cm width) onto the lesions and following withdrawal the furfuraceous scales will remain on the glue side. These pieces are then immersed for some minutes in lactophenol-cotton blue stain. Following absorption of the stain the scales are washed in current water to remove the excess of blue stain, dried with filter paper, dehydrated via passage in two bottles containing absolute alcohol and then placed in xylene in a centrifugation tube. The xylene dissolves the scotch tape glue and the scales fall free in the tube. After centrifugation and decantation the scales concentrated on the bottom of the tube are collected with a platinum-loop, placed in Canada balsam on a microscopy slide and closed with a cover slip. The preparations are then ready to be submitted to microscopic examination. Other stains may also be used instead of lactophenol-cotton blue. This method is simple, easily performed, and offers good conditions to study these fungi as well as being useful for the diagnosis of the diseases that they cause.
Assuntos
Corynebacterium/isolamento & purificação , Eritrasma/microbiologia , Malassezia/isolamento & purificação , Tinha Versicolor/microbiologia , Eritrasma/diagnóstico , Humanos , Coloração e Rotulagem/métodos , Tinha Versicolor/diagnósticoRESUMO
La tinción del PAS más DMSO, es un método sencillo y rápido mediante el cual las raspaduras de lesiones de la piel o de la uñas, recogidas con una laminilla a la que se le ha aplicado albúmina de Mayer en su superficie, puede examinarse microscópicamente y determinar con precisión la ausencia o presencia de hongos y de Corynebacterium minutissimum. El método, además, permite obtener preparaciones permanentes