RESUMO
Polyamines (putrescine, spermidine and spermine) are traditionally implicated in the response of plants to environmental cues. Free spermine accumulation has been suggested as a particular feature of long-term salt stress, and in the model plant Arabidopsis thaliana the spermine synthase gene (AtSPMS) has been reported as inducible by abscisic acid (ABA) and acute salt stress treatments. With the aim to unravel the physiological role of free spermine during salinity, we analyzed polyamine metabolism in A. thaliana salt-hypersensitive sos mutants (salt overlay sensitive; sos1-1, sos2-1 and sos3-1), and studied the salt stress tolerance of the mutants in spermine and thermospermine synthesis (acl5-1, spms-1 and acl5-1/spms-1). Results presented here indicate that induction in polyamine metabolism is a SOS-independent response to salinity and is globally over-induced in a sensitive background. In addition, under long-term salinity, the mutants in the synthesis of spermine and thermospermine (acl5-1, spms-1 and double acl5-1/spms-1) accumulated more Na(+) and performed worst than WT in survival experiments. Therefore, support is given to a role for these higher polyamines in salt tolerance mechanisms.
Assuntos
Arabidopsis/metabolismo , Tolerância ao Sal/fisiologia , Espermina/metabolismo , Arabidopsis/genética , Poliaminas Biogênicas/metabolismo , Regulação da Expressão Gênica de Plantas , Genes de Plantas/efeitos dos fármacos , Variação Genética , Reguladores de Crescimento de Plantas/metabolismo , Salinidade , Tolerância ao Sal/genética , Cloreto de Sódio/metabolismo , Espermina/análogos & derivados , Espermina/biossíntese , Espermina Sintase/genética , Espermina Sintase/metabolismoRESUMO
Trichomonad parasites such as Tritrichomonas foetus produce large amounts of putrescine (1,4-diaminobutane), which is transported out of the cell via an antiport mechanism which results in the uptake of a molecule of spermine. The importance of putrescine to the survival of the parasite and its role in the biology of T. foetus was investigated by use of the putrescine analogue 1, 4-diamino-2-butanone (DAB). Growth of T. foetus in vitro was significantly inhibited by 20 mM DAB, which was reversed by the addition of exogenous 40 mM putrescine. High-performance liquid chromatography analysis of 20 mM DAB-treated T. foetus revealed that putrescine, spermidine, and spermine levels were reduced by 89, 52, and 43%, respectively, compared to those in control cells. The DAB treatment induced several ultrastructural alterations, which were primarily observed in the redox organelles termed hydrogenosomes. These organelles were progressively degraded, giving rise to large vesicles that displayed material immunoreactive with an antibody to beta-succinyl-coenzyme A synthetase, a hydrogenosomal enzyme. A protective role for polyamines as stabilizing agents in the trichomonad hydrogenosomal membrane is proposed.
Assuntos
Poliaminas Biogênicas/biossíntese , Organelas/efeitos dos fármacos , Putrescina/análogos & derivados , Tritrichomonas foetus/efeitos dos fármacos , Tritrichomonas foetus/crescimento & desenvolvimento , Animais , Cromatografia Líquida de Alta Pressão , Meios de Cultura , Microscopia Eletrônica , Movimento/efeitos dos fármacos , Putrescina/biossíntese , Putrescina/farmacologia , Espermidina/biossíntese , Espermina/biossíntese , Tritrichomonas foetus/metabolismo , Tritrichomonas foetus/ultraestruturaRESUMO
This review covers some common aspects of the biosynthesis, interconversion pathways and biochemical functions of polyamines. A particular emphasis is given in experimental models as well as humans, to their presence in the male gonad, prostate gland, seminal vesicles, epididymis and semen. The interaction between hormones (androgens, LH, FSH and PRL) and the main enzymes involved on the polyamine biosynthesis, and the relationship of these compounds on cell growth and differentiation, are also discussed. In this regard, an attention is offered to the potential role of polyamines during early spermatogenesis stages and the use of some enzymes involved in their biosynthesis as sensitive and specific markers of the action of androgens and antiandrogens in the epididymis. Finally, a special issue is addressed to the controversial information documented on polyamines, their oxidation products and the relationship with male fertility.
Assuntos
Poliaminas Biogênicas/fisiologia , Epididimo/metabolismo , Próstata/metabolismo , Sêmen/metabolismo , Glândulas Seminais/metabolismo , Testículo/metabolismo , Acetiltransferases/metabolismo , Animais , Poliaminas Biogênicas/metabolismo , Cricetinae , Humanos , Masculino , Mamíferos , Mesocricetus , Camundongos , Ornitina/metabolismo , Putrescina/biossíntese , Ratos , Espermidina/biossíntese , Espermina/biossínteseRESUMO
This review covers some common aspects of the biosynthesis, interconversion pathways and biochemical functions of polyamines. A particular emphasis is given in experitemtal models as well as humans, to their presence in the male gonad, postate gland, seminal vesicles, epididymis and semen. The interaction between hormones (androgens, LH, FSH and PRL) and the main enzymes involved on the polymine biosynthesis, and the relationship of these compounds on cell growth and differentation, are also discussed. In this regard, an attention is offered to the potential role of polymines during early spermatogenesis stages and the use of some enzymed involved in their biosynthesis as sensitive and specific markers of the action of androgens and antiandrogens in the epididymis. Finally, a special issue is addressed to the controversial information documented on polymines, their oxidation products and the relationship with male fertility.
Assuntos
Humanos , Masculino , Animais , Cricetinae , Camundongos , Ratos , Poliaminas Biogênicas/fisiologia , Epididimo/metabolismo , Ornitina/metabolismo , Próstata/metabolismo , Putrescina/biossíntese , Sêmen/metabolismo , Glândulas Seminais/metabolismo , Espermidina/biossíntese , Espermina/biossíntese , Testículo/metabolismo , Acetiltransferases/metabolismo , Poliaminas Biogênicas/metabolismo , Mamíferos , MesocricetusRESUMO
This review covers some common aspects of the biosynthesis, interconversion pathways and biochemical functions of polyamines. A particular emphasis is given in experitemtal models as well as humans, to their presence in the male gonad, postate gland, seminal vesicles, epididymis and semen. The interaction between hormones (androgens, LH, FSH and PRL) and the main enzymes involved on the polymine biosynthesis, and the relationship of these compounds on cell growth and differentation, are also discussed. In this regard, an attention is offered to the potential role of polymines during early spermatogenesis stages and the use of some enzymed involved in their biosynthesis as sensitive and specific markers of the action of androgens and antiandrogens in the epididymis. Finally, a special issue is addressed to the controversial information documented on polymines, their oxidation products and the relationship with male fertility. (AU)
Assuntos
Humanos , Masculino , Animais , Cricetinae , Camundongos , Ratos , RESEARCH SUPPORT, NON-U.S. GOVT , Poliaminas Biogênicas/fisiologia , Putrescina/biossíntese , Espermidina/biossíntese , Espermina/biossíntese , Ornitina/metabolismo , Testículo/metabolismo , Epididimo/metabolismo , Sêmen/metabolismo , Próstata/metabolismo , Glândulas Seminais/metabolismo , Acetiltransferases/metabolismo , Poliaminas Biogênicas/metabolismo , Mesocricetus , MamíferosRESUMO
2,4-Dichlorophenoxyacetic acid (2,4-D) is a herbicide extensively used in agriculture. It was considered of interest to study its toxicity on animal cells. We had previously determined that 1 mM 2,4-D can inhibit cell growth, DNA and protein synthesis of cultured Chinese hamster ovary cells (CHO) with cell accumulation in the G1/S interphase of the cell cycle. The present work examined the effects of 2,4-D on polyamine biosynthesis. The results suggest some possible mechanism of the herbicide's toxic effects on animal cells.