RESUMO
Abstract Background Current evidence suggests that upregulation of polyamines system plays a role both in cognitive deficit and synaptic loss observed in Alzheimer's disease (AD). Objective The aim of this study was to determine the plasmatic concentration of polyamines in mild cognitive impairment (MCI) and AD patients in comparison with healthy controls (HC). Methods Plasmatic polyamines were quantified using the AbsoluteIDQ® p180 and liquid chromatography coupled to tandem mass spectrometry (LC/MS-MS). Results The study group comprised 34 AD patients, 20 MCI and 25 HC. All individuals were followed for 4 years. During this period 8 amnestic MCI patients (40% of the MCI sample at baseline) converted to AD. Spermidine level was lower in both patient groups (AD; MCI) compared to HC (p = 0.007). Plasma levels of spermine were higher in the MCI group (p < 0.001), but decreased in the sub-sample of MCI patients who converted to AD (p = 0.043). No statistically significant differences were found in ornithine and putrescine levels (p = 0.056 and p = 0.126, respectively). Discussion Our results suggest dynamic changes in the expression of polyamines in the MCI-AD continuum.
Assuntos
Humanos , Masculino , Feminino , Idoso , Idoso de 80 Anos ou mais , Poliaminas/sangue , Espermina/sangue , Doença de Alzheimer/fisiopatologia , Disfunção Cognitiva/fisiopatologia , Ornitina/sangue , Poliaminas/metabolismo , Biomarcadores/sangue , Putrescina/sangue , Espermidina/sangue , Cromatografia Líquida/métodos , Espectrometria de Massas em Tandem/métodos , Metabolômica/métodos , Doença de Alzheimer/diagnóstico , Disfunção Cognitiva/diagnósticoAssuntos
Espermina/sangue , Reações Antígeno-Anticorpo , Feminino , Humanos , Imunoeletroforese , MasculinoRESUMO
Antibodies to Spermine have been prepared in the mouse, rabbit and goat using Sperminated Lysozyme or BSA Spermine as antigens. The reaction between the antiserum and the antigen is inhibited by the tetramine spermine, and the triamine spermidine but not by the diamines putrescine and cadaverine. This antiserum precipates with certain human sera and this has been used as the basis of a qualitative immunoelectrophoretic test for spermine in human sera. It has been found that the sera from patients with cancer and infection give a positive reaction, whilst those from patients with other pathological conditions, examined to date, are negative. The antigen responsible for the precipitation migrates in the region of O2 lipo-proteins with the spermine moiety bond to lipid as a lip-spermine complex. The results are discussed in the light of other lipo-spermine complexes found in human malignant tissue and in bacteria (AU)