RESUMO
Liver extract, plasma from intact mice, ES2 tumour extract and plasma from tumour bearing mice has an inhibiting effect on the mitotic activity of hepatocytes and duodenal enterocytes. In the present experiments, the effect of these treatments on the mitotic activity of renal tubular cells was studied. C3HS 28 day-old male mice, standardized for periodicity analysis were used. The determination of normal mitotic circadian curve of the renocytes was done. A second batch of mice were injected with 0.01 ml/gr of either liver extract, plasma from intact mice, ES2 tumour extract or plasma from tumour bearing mice, at 16:00 hours and controlled at 08:00, 12:00 and 16:00 hs during 2 consecutive days post treatment. Colchicine (2 micrograms/gr) was injected 4 hours before killing. Kidneys were processed for histology and mitotic index determinations. Results were expressed as colchicine metaphases per 1000 nuclei, and showed that mitotic activity values of treated animals were significantly lower than those of controls. In conclusion, mitotic activity inhibition of renocytes may be due to some non specific plasmatic and/or tissue factors.
Assuntos
Túbulos Renais/citologia , Plasma , Extratos de Tecidos/farmacologia , Animais , Divisão Celular/efeitos dos fármacos , Fator de Crescimento de Hepatócito/farmacologia , Túbulos Renais/efeitos dos fármacos , Extratos Hepáticos/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos C3H , Mitose/fisiologia , Neoplasias Experimentais/sangue , Extratos de Tecidos/químicaRESUMO
Liver extract, plasma from intact mice, ES2 tumour extract and plasma from tumour bearing mice has an inhibiting effect on the mitotic activity of hepatocytes and duodenal enterocytes. In the present experiments, the effect of these treatments on the mitotic activity of renal tubular cells was studied. C3HS 28 day-old male mice, standardized for periodicity analysis were used. The determination of normal mitotic circadian curve of the renocytes was done. A second batch of mice were injected with 0.01 ml/gr of either liver extract, plasma from intact mice, ES2 tumour extract or plasma from tumour bearing mice, at 16:00 hours and controlled at 08:00, 12:00 and 16:00 hs during 2 consecutive days post treatment. Colchicine (2 micrograms/gr) was injected 4 hours before killing. Kidneys were processed for histology and mitotic index determinations. Results were expressed as colchicine metaphases per 1000 nuclei, and showed that mitotic activity values of treated animals were significantly lower than those of controls. In conclusion, mitotic activity inhibition of renocytes may be due to some non specific plasmatic and/or tissue factors.
Assuntos
Animais , Masculino , Camundongos , Plasma , Extratos de Tecidos , Túbulos Renais/citologia , Divisão Celular/efeitos dos fármacos , Extratos Hepáticos/farmacologia , Fator de Crescimento de Hepatócito/farmacologia , Mitose , Neoplasias Experimentais , Extratos de Tecidos , Túbulos Renais/efeitos dos fármacosRESUMO
Liver extract, plasma from intact mice, ES2 tumour extract and plasma from tumour bearing mice has an inhibiting effect on the mitotic activity of hepatocytes and duodenal enterocytes. In the present experiments, the effect of these treatments on the mitotic activity of renal tubular cells was studied. C3HS 28 day-old male mice, standardized for periodicity analysis were used. The determination of normal mitotic circadian curve of the renocytes was done. A second batch of mice were injected with 0.01 ml/gr of either liver extract, plasma from intact mice, ES2 tumour extract or plasma from tumour bearing mice, at 16:00 hours and controlled at 08:00, 12:00 and 16:00 hs during 2 consecutive days post treatment. Colchicine (2 micrograms/gr) was injected 4 hours before killing. Kidneys were processed for histology and mitotic index determinations. Results were expressed as colchicine metaphases per 1000 nuclei, and showed that mitotic activity values of treated animals were significantly lower than those of controls. In conclusion, mitotic activity inhibition of renocytes may be due to some non specific plasmatic and/or tissue factors.(AU)
Assuntos
Animais , Masculino , Camundongos , Túbulos Renais/citologia , Plasma , Extratos de Tecidos/farmacologia , Divisão Celular/efeitos dos fármacos , Fator de Crescimento de Hepatócito/farmacologia , Túbulos Renais/efeitos dos fármacos , Extratos Hepáticos/farmacologia , Camundongos Endogâmicos C3H , Mitose/fisiologia , Neoplasias Experimentais/sangue , Extratos de Tecidos/químicaRESUMO
We have previously demonstrated that adult mouse plasma obtained 36 hours post partial hepatectomy has an inhibitory effect on the mitotic activity of enterocytes from young mouse duodenal cripts. In this paper we investigate whether this effect is derived from any regenerating liver factor. Accordingly, we studied the action of adult mouse (90 days old) liver extract obtained 36 hours after partial hepatectomy (70%), on the mitotic activity of young mouse enterocytes, considering 3 cellular levels of the duodenum cripts. Thirty six C3H/S inbred female mice (27 days old) were employed. Half of them received at 16:00 hour an intraperitoneal injection of saline and the other half received liver extract (0.01 ml/g). Animals from each group were sacrificed at 08:00/ 16, 12:00/20 and 16:00/24 (time of day/hours post treatment). All the animals received an intraperitoneal dose of colchicine (2 micrograms/g) 4 hours before sacrifice. The results are expressed as colchicine metaphases/1000 nuclei and show that the mitotic activity is significantly lower in the animals treated with the extract than in the controls. This inhibiting effect is observed at the levels from 1 to 4 and from 5 to 12 cells of the analyzed cripts. At the superficial level from 13 to 20 cells there is no modification of the proliferative activity. This inhibiting effect on the mitotic activity of duodenum enterocytes from the basal and intermedial zone of the cripts is probably due to a liver diffusing factor.
Assuntos
Duodeno/citologia , Hepatectomia , Extratos Hepáticos/farmacologia , Mitose/efeitos dos fármacos , Animais , Camundongos , Camundongos Endogâmicos C3HRESUMO
En un trabajo anterior demonstramos que el plasma de retones adultos obtenido 36 horas post hepatectomía parcial, ejerce un efecto inhibitorio en la actividad mitótica de los enterocitos crípticos duodenales del ratón joven. En el presente trabajo se analiza la posibilidad de que dicho efecto se origine en algún factor del hígado regenerante. Para ello se estudia la acción del extracto de hígado de ratones adultos (90 días) obtenido 36 horas después de su hepatectomía parcial (70 por ciento), sobre la actividad mitótica de los enterocitos de ratones jóvenes, analizando 3 niveles celulares de las criptas duodenales. Se emplearon 36 ratones hembra de la cepa C3H/S de 27 días de edad la mitad de los cuales recibió, a las 16:00 horas, una inyección intraperitoneal de solución fisiológica, y restantes extracto hepático (0,01 ml/g). Lotes de 8 animales de cada grupo se sacrificaron a las 08:00/16, 12:00/20 y 16:00/24 (hora del día/horas post tratamiento) previa inyección de colchicina (2mug/g) 4 horas antes. Los resultados, expresados como metafases colchicínas por mil núcleos, demuenstran que la actividad mitótica, en los animales tratados con extracto, es significativamente menor con respecto a los testigos. El efecto inhibidor se manifesta en los niveles celulares de 1 a 4 y de 5 a 12 células de las criptas analizadas. En el nivel superior, de 13 a 20 células, no se aprecia ninguna modificación de la actividad proliferativa. Esta inhibición de la actividad mitótica de los enterocitos de las zonas basal y media de las criptas duodenales es probablemente debido a factores hepáticos difusibles.
Assuntos
Camundongos , Animais , Duodeno/citologia , Hepatectomia , Técnicas In Vitro , Extratos Hepáticos/farmacologia , Mitose/efeitos dos fármacos , Camundongos Endogâmicos C3HRESUMO
En un trabajo anterior demonstramos que el plasma de retones adultos obtenido 36 horas post hepatectomía parcial, ejerce un efecto inhibitorio en la actividad mitótica de los enterocitos crípticos duodenales del ratón joven. En el presente trabajo se analiza la posibilidad de que dicho efecto se origine en algún factor del hígado regenerante. Para ello se estudia la acción del extracto de hígado de ratones adultos (90 días) obtenido 36 horas después de su hepatectomía parcial (70 por ciento), sobre la actividad mitótica de los enterocitos de ratones jóvenes, analizando 3 niveles celulares de las criptas duodenales. Se emplearon 36 ratones hembra de la cepa C3H/S de 27 días de edad la mitad de los cuales recibió, a las 16:00 horas, una inyección intraperitoneal de solución fisiológica, y restantes extracto hepático (0,01 ml/g). Lotes de 8 animales de cada grupo se sacrificaron a las 08:00/16, 12:00/20 y 16:00/24 (hora del día/horas post tratamiento) previa inyección de colchicina (2mug/g) 4 horas antes. Los resultados, expresados como metafases colchicínas por mil núcleos, demuenstran que la actividad mitótica, en los animales tratados con extracto, es significativamente menor con respecto a los testigos. El efecto inhibidor se manifesta en los niveles celulares de 1 a 4 y de 5 a 12 células de las criptas analizadas. En el nivel superior, de 13 a 20 células, no se aprecia ninguna modificación de la actividad proliferativa. Esta inhibición de la actividad mitótica de los enterocitos de las zonas basal y media de las criptas duodenales es probablemente debido a factores hepáticos difusibles. (AU)