RESUMO
A new species of rhynchobdellid leech, Placobdella ringueleti n. sp., is described based on the examination of 25 specimens. Leeches were collected from body appendages of freshwater turtles (Kinosternon leucostomum, Dermatemys mawii, and Staurotypus triporcatus ) from Chiapas, Mexico. The new species resembles other members of the genus in the ocular morphology, bilobated ovaries, and presence of 1 pair of elongated mycetomes, but it is distinguished from them by the dorsal papillar pattern, by having a continuous longitudinal mid-dorsal stripe along the dorsal surface and papillae on the dorsal surface of the posterior sucker. Placobdella ringueleti represents the third species of the genus in Mexico. The type locality of the new species is part of the "El Ocote" Biosphere Reserve, Chiapas, Mexico.
Assuntos
Sanguessugas/classificação , Tartarugas/parasitologia , Animais , Extremidades/parasitologia , Água Doce , Sanguessugas/anatomia & histologia , México , Pele/parasitologiaRESUMO
Trypanosoma cruzi, the protozoan that causes Chagas' heart disease, invades endothelial cells in vitro by activating the B2 kinin receptor (B2R). Here, we demonstrate that mice infected with trypomastigotes develop potent edema after treatment with the angiotensin-converting enzyme (ACE) (or kininase II) inhibitor captopril. Experiments performed with specific kinin receptor (B2R/B1R) antagonists and knockout mice revealed that the early-phase (3-h) edema is mediated by the constitutive B2R, whereas the late-phase (24-h) response depends on stimulation of the up-regulated B1R. Given previous evidence that parasite invasion of cells expressing B2R is potentiated by captopril, we investigated the prerequisites for in vitro infection of Chinese hamster ovary cells overexpressing either B1R or B2R, human umbilical vein endothelial cells activated by lipopolysaccharide, and neonatal rat cardiomyocytes. Our results indicate that captopril potentiates parasite invasion regardless of the kinin (B2/B1) activation pathways, whereas DL-2-mercaptomethyl-3-guanidino-ethylthiopropanoic acid (MGTA), an inhibitor of kininase I (carboxypeptidase M/N), selectively decreases parasite infectivity for B1R-expressing cells. These data suggest that formation of the B1R agonist, i.e., [des-Arg] kinins, critically depends on the processing action of kininase I, here proposed as a potential pathogenesis cofactor. Collectively, our data suggest that fluctuations in the levels of kininases may modulate parasite infectivity and pathological outcome in Chagas' disease.
Assuntos
Doença de Chagas/etiologia , Edema/parasitologia , Coração/parasitologia , Receptores da Bradicinina/metabolismo , Trypanosoma cruzi/patogenicidade , Animais , Animais Recém-Nascidos , Células CHO , Células Cultivadas , Doença de Chagas/metabolismo , Cricetinae , Edema/metabolismo , Endotélio Vascular/parasitologia , Extremidades/parasitologia , Cinética , Lipopolissacarídeos/farmacologia , Lisina Carboxipeptidase/fisiologia , Camundongos , Modelos Biológicos , Receptor B1 da Bradicinina , Receptor B2 da Bradicinina , Receptores da Bradicinina/fisiologia , Trypanosoma cruzi/crescimento & desenvolvimentoRESUMO
Mites collected from the auditory canal of Cebus apella (capuchin monkey), family Cebidae, were identified as Fonsecalges johnjadini (Psoroptidae, Cebalginae). It is the first record of this parasite from this monkey. This paper emphasizes the importance of clinical and anatomopathological examinations for parasitic diagnosis in wild animals.
Assuntos
Cebus/parasitologia , Infestações por Ácaros/patologia , Ácaros/classificação , Doenças Parasitárias em Animais/patologia , Animais , Orelha Externa/parasitologia , Orelha Externa/patologia , Extremidades/parasitologia , Extremidades/patologia , Feminino , Hiperplasia , Lábio/parasitologia , Lábio/patologia , Masculino , Pele/parasitologia , Pele/patologiaRESUMO
Mites collected from the auditory canal of Cebus apella (capuchin monkey), family Cebidae, were identified as Fonsecalges johnjadini (Psoroptidae, Cebalginae). It is the first record of this parasite from this monkey. This paper emphasizes the importance of clinical and anatomopathological examinations for parasitic diagnosis in wild animals