RESUMO
Background: Fascioliasis is an infectious disease caused by parasites Fasciola hepatica and F. gigantica. Humans are infected by the consumption of vegetables and water contaminated with the infective form of the parasite. Materials and Methods: In this study, an IgM-ELISA with the cysteine proteinase Fas2 antigen was evaluated with sera from 76 patients infected with F. hepatica, 24 patients with other parasite infections and 84 healthy volunteers. Results: IgM-ELISA resulted in 43% positives in F. hepatica patients with positive serology to Fas2-ELISA, but no positives resulted from testing healthy volunteers and individuals infected with other parasites. The IgM-ELISA diagnostic parameters showed a sensitivity of 43.4% (95% CI 0.321-0.553), specificity of 100% (95% CI 0.957-1), and no cross-reactivity with other parasitic infection. Interference by rheumatoid factor in the IgM immunoassay was controlled by treating sera with rheumatoid factor absorbent before testing. Conclusions: Fas2 antigen is detected by circulating IgM in patients infected with F. hepatica and IgM-ELISA using Fas2 appears as a specific immunoassay to detect the acute phase of the acute phase of F. hepatica infection in humans.
Assuntos
Anticorpos Anti-Idiotípicos/sangue , Anticorpos Anti-Helmínticos/sangue , Antígenos de Helmintos/sangue , Cisteína Endopeptidases/sangue , Fasciola hepatica/imunologia , Fasciolíase/sangue , Imunoglobulina G/sangue , Imunoglobulina M/imunologia , Animais , Área Sob a Curva , Ensaio de Imunoadsorção Enzimática , Fasciola hepatica/enzimologia , Fasciolíase/imunologia , Humanos , Fatores Imunológicos/farmacologia , Peru , Valor Preditivo dos Testes , Curva ROC , Fator Reumatoide/farmacologia , Sensibilidade e Especificidade , Estudos SoroepidemiológicosRESUMO
Circulating complexes in leprosy studied by the platelet aggregation test. The platelet aggregation test and its relation to the Rubino test and other sero-immunological parameters in 135 patients with leprosy.