RESUMO
Helicobacter pylori is a microorganism that infects 60% of the population and is considered the main cause of atrophic gastritis, gastric and duodenal ulcers, and gastric cancer. Different emerging pathogens have been found in drinking water and their presence is considered to be an important public health problem. For this reason, it is necessary to carry out the validation of reliable technologies for this type of pathogens and evaluate their performance. This paper reports, for the first time, H. pylori reduction in a drinking water pilot plant of two slow sand filters (SSF). Inlet water was taken from a gravel filtration system of a rural water supply in Colombia and then inoculated with viable cells of H. pylori. By determining the Genomic Units (GU) through quantitative Polymerase Chain Reaction (qPCR), the concentration of GU/sample was measured. In the inlet water amplification for SSF1 and SSF2 were 5.13 × 102 ± 4.48 × 102 and 6.59 × 102 ± 7.32 × 102, respectively, while for the treated water they were 7.0 ± 5.6 and 2.05 × 101 ± 2.9 × 101 GU/sample for SSF1 and SSF2, respectively. The SSF pilot plant reached up to 3 log reduction units of H. pylori; therefore, since there is not an H. pylori contamination indicator and its periodic monitoring is financially complicated, the SSF could guarantee the drinking water quality necessity that exists in rural areas and small municipalities in developing countries, where infection rates and prevalence of this pathogen are high.
Assuntos
Água Potável , Filtração , Helicobacter pylori , Microbiologia da Água , Purificação da Água , Abastecimento de Água , Filtração/métodos , Água Potável/microbiologia , Purificação da Água/métodos , Areia , ColômbiaRESUMO
The increased production of polystyrene waste has led to the need to find efficient ways to dispose of it. One possibility is the use of solid waste to produce filter media by the electrospinning technique. The aim of this work was to develop an ultra-fast electrospinning process applied to recycled polystyrene, with statistical evaluation of the influence of polymeric solution parameters (polymer concentration and percentage of DL-limonene) and process variables (flow rate, voltage, and type of support) on nanoparticle collection efficiency, air permeability, and fiber diameter. An extensive characterization of the materials and evaluation of the morphology of the fibers was also carried out. It was found that recycled expanded polystyrene could be used in electrospinning to produce polymeric membranes. The optimized condition that resulted in the highest nanoparticle collection efficiency was a polymer concentration of 13.5%, percentage of DL-limonene of 50%, voltage of 25 kV, and flow rate of 1.2 mL/h, resulting in values of 99.97 ± 0.01%, 2.6 ± 0.5 × 10-13 m2, 0.19 Pa-1, and 708 ± 176 nm for the collection efficiency of nanoparticles in the range from 6.38 to 232.9 nm, permeability, quality factor, and mean fiber diameter, respectively. All the parameters were found to influence collection efficiency and fiber diameter. The use of DL-limonene, a natural solvent, provided benefits including increased collection efficiency and decreased fiber size. In addition, the electrostatic filtration mechanism was evaluated using the presence of a copper grid as a support for the nanofibers. The findings demonstrated that an electrospinning time of only 5 min was sufficient to obtain filters with high collection efficiencies and low pressure drops, opening perspectives for the application of polystyrene waste in the development of materials with excellent characteristics for application in the area of atmospheric pollution mitigation.
Assuntos
Filtração , Nanopartículas , Poliestirenos , Poliestirenos/química , Nanopartículas/química , Filtração/métodos , Membranas Artificiais , Polímeros/química , Reciclagem , PermeabilidadeRESUMO
As global effects of water scarcity raise concerns and environmental regulations evolve, contemporary wastewater treatment plants (WWTPs) face the challenge of effectively removing a diverse range of contaminants of emerging concern (CECs) from municipal effluents. This study focuses on the assessment of advanced oxidation processes (AOPs), specifically UV-C/H2O2 and UV-C/Chlorine, for the removal of 14 target CECs in municipal secondary effluent (MSE, spiked with 10 µg L-1 of each CEC) or in the subsequent MSE nanofiltration retentate (NFR, no spiking). Phototreatments were carried out in continuous mode operation, with a hydraulic retention time of 3.4 min, using a tube-in-tube membrane photoreactor. For both wastewater matrices, UV-C photolysis (3.3 kJ L-1) exhibited high efficacy in removing CECs susceptible to photolysis, although lower treatment performance was observed for NFR. In MSE, adding 10 mg L-1 of H2O2 or Cl2 enhanced treatment efficiency, with UV-C/H2O2 outperforming UV-C/Chlorine. Both UV-C/AOPs eliminated the chronic toxicity of MSE toward Chlorella vulgaris. In the NFR, not only was the degradation of target CECs diminished, but chronic toxicity to C. vulgaris persisted after both UV-C/AOPs, with UV-C/Chlorine increasing toxicity due to potential toxic by-products. Nanofiltration permeate (NFP) exhibited low CECs and microbial content. A single chlorine addition effectively controlled Escherichia coli regrowth for 3 days, proving NFP potential for safe reuse in crop irrigation (<1 CFU/100 mL for E. coli; <1 mg L-1 for free chlorine). These findings provide valuable insights into the applications and limitations of UV-C/H2O2 and UV-C/Chlorine for distinct wastewater treatment scenarios.
Assuntos
Cloro , Filtração , Peróxido de Hidrogênio , Fotólise , Raios Ultravioleta , Eliminação de Resíduos Líquidos , Águas Residuárias , Poluentes Químicos da Água , Peróxido de Hidrogênio/química , Águas Residuárias/química , Poluentes Químicos da Água/química , Poluentes Químicos da Água/análise , Eliminação de Resíduos Líquidos/métodos , Cloro/química , Filtração/métodos , Purificação da Água/métodos , Chlorella vulgaris/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , OxirreduçãoRESUMO
This research investigated the removal of contaminants of emerging concern (CECs) and characterized the microbial community across an advanced water treatment (AWT) train consisting of Coagulation/Flocculation/Clarification/Granular Media Filtration (CFCGMF), Ozone-Biological Activated Carbon Filtration (O3/BAC), Granular Activated Carbon filtration, Ultraviolet Disinfection, and Cartridge Filtration (GAC/UV/CF). The AWT train successfully met the goals of CECs and bulk organics removal. The microbial community at each treatment step of the AWT train was characterized using 16S rRNA sequencing on the Illumina MiSeq platform generated from DNA extracted from liquid and solid (treatment media) samples taken along the treatment train. Differences in the microbial community structure were observed. The dominant operational taxonomic units (OTU) decreased along the treatment train, but the treatment steps did impact the microbial community composition downstream of each unit process. These results provide insights into microbial ecology in advanced water treatment systems, which are influenced and shaped by each treatment step, the microbial community interactions, and their potential metabolic contribution to CECs degradation.
Assuntos
Água Potável , Ozônio , Poluentes Químicos da Água , Purificação da Água , Carvão Vegetal/química , RNA Ribossômico 16S , Poluentes Químicos da Água/química , Purificação da Água/métodos , Filtração/métodos , Ozônio/químicaRESUMO
In the cheese industry, whey, which is rich in lactose and proteins, is underutilized, causing adverse environmental impacts. The fractionation of its components, typically carried out through filtration membranes, faces operational challenges such as membrane fouling, significant protein loss during the process, and extended operating times. These challenges require attention and specific methods for optimization and to increase efficiency. A promising strategy to enhance industry efficiency and sustainability is the use of enzymatic pre-treatment with the enzyme transglutaminase (TGase). This enzyme plays a crucial role in protein modification, catalyzing covalent cross-links between lysine and glutamine residues, increasing the molecular weight of proteins, facilitating their retention on membranes, and contributing to the improvement of the quality of the final products. The aim of this study is to review the application of the enzyme TGase as a pretreatment in whey protein filtration. The scope involves assessing the enzyme's impact on whey protein properties and its relationship with process performance. It also aims to identify both the optimization of operational parameters and the enhancement of product characteristics. This study demonstrates that the application of TGase leads to improved performance in protein concentration, lactose permeation, and permeate flux rate during the filtration process. It also has the capacity to enhance protein solubility, viscosity, thermal stability, and protein gelation in whey. In this context, it is relevant for enhancing the characteristics of whey, thereby contributing to the production of higher quality final products in the food industry. © 2023 Society of Chemical Industry.
Assuntos
Queijo , Soro do Leite , Proteínas do Soro do Leite/química , Soro do Leite/metabolismo , Transglutaminases/metabolismo , Lactose , Filtração/métodos , Queijo/análiseRESUMO
This study investigated the performance of a granular filtration system (GFS) composed of a rock filter (RF), a rapid sand filter (RSF), and an activated carbon filter (ACF), applied to the post-treatment of an anaerobic reactor effluent. Four filtration rates (FR) were applied to the GFS (in m3·m-2·d-1): 100-60-60, 100-90-90, 200-120-120, and 200-160-160, for RF-RSF-ACF, respectively. A clarified final effluent with low turbidity (~ 10 NTU), solids (~ 6.5 mg TSS.L-1), and organic matter content (~ 40 mg COD.L-1) was obtained when the GFS worked with FR up to 100-90-90 m3·m-2·d-1. For higher FR, the effluent quality was a little poorer. Principal component analysis showed when the RSF operated at 120 or 160 m3·m-2·d-1, it presented an effluent with higher turbidity which did not affect negatively the ACF performance. The hydraulic load limits in the RSF were reached in periods of 45, 30, and 24.5 h for the FR of 60, 120, and 160 m3·m-2·d-1, respectively, and head loss analysis depicted a more distributed solid retention through the sand depth with the lower FR. Thus, the results revealed that the RF-RSF-ACS system is a promising alternative for effluent polishing of anaerobic reactor, especially when the FR is set at 90 m3·m-2·d-1 or even higher.
Assuntos
Eliminação de Resíduos Líquidos , Purificação da Água , Eliminação de Resíduos Líquidos/métodos , Anaerobiose , Carvão Vegetal , Filtração/métodos , Purificação da Água/métodosRESUMO
Due to the recent coronavirus-2019 pandemic, several studies have emerged looking for new materials, especially with biocidal characteristics. Thus, the present research investigates the antibacterial properties of biodegradable cellulose acetate (CA) / cetylpyridinium bromide (CPB) electrospun nanofibers, their aerosol filtration, and the possible use as a filter media of surgical face masks. Then, samples of these nanofibers were produced over a nonwoven substrate, using different volumes of polymeric solution during the electrospinning process. The evaluation of the antibacterial properties of the nanofibers was performed for Escherichia coli and Staphylococcus aureus using quantitative methods. The aerosol filtration performance was evaluated in these samples for NaCl nanoparticles (from 7-300â nm) and with 8â mL min-1 of air flow rate. The results show that the single use of the surfactant has antibacterial properties from a concentration of 39â µg mL-1 of solution. The nanofibers presented a reduction of 100% for both bacteria. Air filtration tests showed 126.03 and 207.73 Pa cm-² of pressure drops and 63 and 77% of aerosol filtration efficiency (FE) for samples with 0.13 and 0.15 mL, respectively. Regarding the nanofiber produced with 0.35 mL, the value obtained was 115.13 ± 33.64 Pa cm-2 and 3.15% of particle penetration. These breathability values are higher than those required for the surgical face mask standard, indicating that improvements in the porosity and thickness are necessary to meet the Brazilian requirements. However, the nanofibers could be applied as filter media for indoor air conditioning systems due to their FE and biocidal properties.
Assuntos
Filtros de Ar , Nanofibras , Nanofibras/química , Máscaras , Filtração/métodos , Antibacterianos , Aerossóis , Escherichia coliRESUMO
Fecal contamination in natural water sources is a common problem in low-income countries. Several health risks are associated with unprotected water sources, such as gastrointestinal infections caused by parasites, viruses, and bacteria. Moreover, antibiotic-resistant bacteria in water sources have become an increasing problem worldwide. This study aimed to evaluate the bacterial pathogens present in water within a rural context in Ecuador, along with the efficiency of black ceramic water filters (BCWFs) as a sustainable household water treatment. We monitored five natural water sources that were used for human consumption in the highlands of Ecuador and analyzed the total coliforms and E. coli before and after BCWF installation. The results indicated a variable bacterial contamination (29-300 colony-forming units/100mL) in all unfiltered samples, and they were considered as high risk for human consumption, but after filtration, no bacteria were present. Moreover, extended-spectrum beta-lactamase-producing E. coli with blaTEM, blaCTX-M9, and blaCTX-M1 genes, and two E. coli classified in the clonal complex ST10 (ST98) were detected in two of the locations sampled; these strains can severely impact public health. The clonal complex ST10, found in the E. coli isolates, possesses the potential to spread bacteria-resistant genes to humans and animals. The results of the use of BCWFs, however, argue for the filters' potential impact within those contexts, as the BCWFs completely removed even antibiotic-resistant contaminants from the water.
Assuntos
Água Potável , Infecções por Escherichia coli , Escherichia coli , Filtração , Animais , Cerâmica , Água Potável/microbiologia , Farmacorresistência Bacteriana , Equador , Infecções por Escherichia coli/microbiologia , Infecções por Escherichia coli/prevenção & controle , Filtração/métodos , Humanos , beta-Lactamases/genéticaRESUMO
This study evaluated clogging effects on head loss and water quality in sand prefiltration columns, followed by innovative double-layered slow-filter filtration technology using anthracite coal and sand. The study was divided into two phases. Phase 1 (filtration rate 11 m3 /m2 ·d) had a layout of a pre-filter (PF) system followed by a slow filter (SF). In Phase 2 (filtration rates of 5.5 and 11 m3 /m2 ·d), two PF systems (PF1 and PF2) followed by SFs (SF1 and SF2) were monitored, be differed only by filtration rate. Phase 1 presents a mean turbidity removal of 45.6% in PF and 23.9% in SF. PF presented a lower head loss in the superficial layers of the filter media, whereas SF presented a more important contribution in the deeper layers of the bed. In Phase 2, the mean results obtained for turbidity removal in PF1 and SF1 were 84.7% and 22.4%, in that order. PF2 and SF2 promoted mean reductions of 84.5% and 22.0%, respectively. Greater clogging is observed in the PF's surface layers, whereas the SFs dissipate greater clogging with depth. Filtration rates and water quality were similar in filter media of different compositions. PRACTITIONER POINTS: Double-layer filters show better use of the porous media in depth. Different materials produce different forms of clogging. Higher filtration rates increase the number of pre-filter and slow filter runs.
Assuntos
Purificação da Água , Qualidade da Água , Carvão Mineral , Meios de Cultura , Filtração/métodos , Purificação da Água/métodosRESUMO
This study aimed to evaluate the efficiency of four household slow sand filter (HSSF) models for the removal of microorganisms from river water throughout the development of their biological layers (schmutzdecke). Two models were designed to be operated continuously (HSSF-CC and HSSF-CT) and two intermittently (HSSF-ID and HSSF-IF). Filters were fed daily with 48 L pre-treated river water (24â h sedimentation followed by filtration through a non-woven synthetic blanket). Water samples were quantified by coliform group bacteria and analysed by bright field microscopy to visualize the microorganisms. Total coliform reduction was between 1.42 ± 0.59 log and 2.96 ± 0.58 log, with continuous models showing a better performance (p-values < 0.004). Escherichia coli reduction varied from 1.49 ± 0.58 log to 2.09 ± 0.66 log and HSSF-IF, HSSF-CC and HSSF-CT presented a similar performance (p-values > 0.06), slightly better than the one presented by HSSF-ID (p-value=0.04). Microorganisms, such as algae, protozoa and helminths were detected by microscopy in raw water and pre-treated water. Algae were the most significant group in these samples, although they were not visualized by bright field microscopy in the filtered water. Results showed the potential of HSSF in microbiological risk reduction from river water, which increases the range of point-of-use water treatments in rural communities. However, additional studies of the HSSF biological layer must be performed.