RESUMO

In this study, the physio pathological effects of Aspergillus alliaceus (Aa, fungi, biocontrol agent) on Orobanche (parasitic plant) were investigated by hormone and phenolic substance tests. In experimental group, Orobanches were treated with the fungi, considering control group was fungus-free. Based on the hormonal tests, in the experimental group, salicylic acid (SA), jasmonic acid (JA), abscisic acid (ABA) and gibberellic acid (GA) levels significantly decreased, and only indole acetic acid (IAA) hormone levels were fairly higher than the control group. According to phenolic substance tests, it was found that only gallic acid, syringic acid and caffeic acid values significantly increased compared with control, and catechin and p-coumaric acid values were significantly lower. Consequently, it was determined that Aa pathogenesis (1) considerably reduces the effects of all defence hormones (JA, ABA, SA), (2) operates an inadequate defence based solely on the IAA hormone and several phenolic substances (gallic acid, syringic acid and caffeic acid), (3) and inevitably the fungi lead the Orobanche to a slow and continuous death. The results were evaluated in detail in the light of similar recent article and current literature in terms of biocontrol and pathology.

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The establishment of invasive plants negatively affects natural environments. Invasive herbivores that attack weeds can be used as a form of biological control, but natural enemies of herbivores must be associated with this interaction to prevent the invasive phytophagous from become a local pest. We performed a greenhouse experiment to evaluate how the cotton aphid, Aphis gossypii, a ok and invasive herbivore, affects the performance of the weed Tithonia diversifolia, the Mexican sunflower. We also examined the relationship between the aphid and local natural enemies. Seedlings of T. diversifolia were divided in two groups: one infested by the aphid and another not infested. After 22 days, we assessed the relationship between aphid abundance and the presence of natural enemies (Coccinelidae and Aphidius platensis) on infested plants, and compared the vegetative performance of the two seedling groups. Both natural enemies were positively related to high aphid density on infested plants. Plants infested by the aphid presented foliar necrosis and senescence, and a reduction of around 50% in leaf number, foliar area, shoot length and shoot, root and total plant weight compared to non-infested plants. These results indicate potential biological control of Mexican sunflower seedlings by the cotton aphid, and control of this aphid by the studied natural enemies.(AU)

Assuntos

RESUMO

The establishment of invasive plants negatively affects natural environments. Invasive herbivores that attack weeds can be used as a form of biological control, but natural enemies of herbivores must be associated with this interaction to prevent the invasive phytophagous from become a local pest. We performed a greenhouse experiment to evaluate how the cotton aphid, Aphis gossypii, a ok and invasive herbivore, affects the performance of the weed Tithonia diversifolia, the Mexican sunflower. We also examined the relationship between the aphid and local natural enemies. Seedlings of T. diversifolia were divided in two groups: one infested by the aphid and another not infested. After 22 days, we assessed the relationship between aphid abundance and the presence of natural enemies (Coccinelidae and Aphidius platensis) on infested plants, and compared the vegetative performance of the two seedling groups. Both natural enemies were positively related to high aphid density on infested plants. Plants infested by the aphid presented foliar necrosis and senescence, and a reduction of around 50% in leaf number, foliar area, shoot length and shoot, root and total plant weight compared to non-infested plants. These results indicate potential biological control of Mexican sunflower seedlings by the cotton aphid, and control of this aphid by the studied natural enemies.

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Insect pollination is issential for hybrid seed production systems, among which, introduced and native bees are the primary pollinating agents transferring pollen from male fertile (MF) to male sterile (MS) lines. On a highly dimorphic sunflower (Helianthus annuus) crop, we assessed the foraging behavior of solitary Melissodes bees and honey bees Apis mellifera. We found that Melissodes spp. were dominant in and showed fidelity to MF plants, gathering sunflower pollen efficiently throughout the day. In contrast, honey bees dominated on MS lines, mostly gathered nectar and exhibited high floral constancy, even after interacting with a second visitor. Also, honey bees carried sunflower pollen on their bodies while visiting MS inflorescences. This study highlights the need for a thorough understanding of the factors involved in a pollinator-dependent agroecosystem crop to assess the contribution of native bees on pollination of crops which offer resources spatially separated in two highly dimorphic parental lines.

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O objetivo da pesquisa foi avaliar a severidade da mancha-de-alternaria (Alternaria helianthi) na cultura do girassol semeado em diferentes densidades populacionais. Os experimentos foram conduzidos na Fazenda Experimental da Ressacada, em Florianópolis, SC, no ano agrícola 2009/2010. A semeadura foi efetuada com espaçamento de 0,5 e 1,0 m entre as linhas de plantio, mantendo-se 3,7; 4,9; 5,7 e 6,6 plantas por metro, resultando em 74 mil, 98 mil, 114 mil, 132 mil plantas por hectare para 0,5 m entre as linhas de plantio e 37 mil, 49 mil, 57 mil e 66 mil plantas por hectare para o espaçamento de 1,0 m. O delineamento experimental utilizado foi de blocos ao acaso, em esquema fatorial 4 x 2 (quatro densidades de semeadura e dois espaçamentos entre linhas) com três repetições. A severidade foi avaliada com base em escala diagramática específica para mancha-de-alternaria em girassol. Os resultados indicaram não haver interação entre os fatores espaçamento entre linhas e densidade de semeadura na severidade da mancha-de-alternaria, mas mostraram haver efeito da densidade de plantio na severidade da doença, com os níveis de severidade aumentando juntamente ao aumento no número de plantas na linha de plantio, para os dois espaçamentos utilizados.

The aim of this research was to evaluate the severity of alternaria leaf spot (Alternaria helianthi) in sunflower crop sown in different densities. The experiments were conducted at the Ressacada Experimental Farm, Florianopolis, SC, Brazil, on the crop season of 2009/2010. Sowing was done at a spacing of 0.5 and 1.0 m between the rows, keeping 3.7, 4.9, 5.7 and 6.6 plants per meter, resulting in 74,000; 98,000; 114,000 and 132,000 plants per hectare to 0.5 m between the tree rows and 37,000; 49,000; 57,000 and 66,000 plants per hectare for the spacing of 1.0 m. The experimental design was in randomized blocks in a 4 x 2 factorial (four seeding rates and two row spacings) with three replicates. The severity was assessed based on diagrammatic scale for specific alternaria leaf spot in sunflower. The results indicated no interaction between the levels of severity with the variation of line spacing and distribution of plants on line, but showed effect of the distribution of plants on line in the severity, with the higher plant density in the line showed a higher severity, for the both spacing in the lines planting.

Assuntos

RESUMO

O objetivo da pesquisa foi avaliar a severidade da mancha-de-alternaria (Alternaria helianthi) na cultura do girassol semeado em diferentes densidades populacionais. Os experimentos foram conduzidos na Fazenda Experimental da Ressacada, em Florianópolis, SC, no ano agrícola 2009/2010. A semeadura foi efetuada com espaçamento de 0,5 e 1,0 m entre as linhas de plantio, mantendo-se 3,7; 4,9; 5,7 e 6,6 plantas por metro, resultando em 74 mil, 98 mil, 114 mil, 132 mil plantas por hectare para 0,5 m entre as linhas de plantio e 37 mil, 49 mil, 57 mil e 66 mil plantas por hectare para o espaçamento de 1,0 m. O delineamento experimental utilizado foi de blocos ao acaso, em esquema fatorial 4 x 2 (quatro densidades de semeadura e dois espaçamentos entre linhas) com três repetições. A severidade foi avaliada com base em escala diagramática específica para mancha-de-alternaria em girassol. Os resultados indicaram não haver interação entre os fatores espaçamento entre linhas e densidade de semeadura na severidade da mancha-de-alternaria, mas mostraram haver efeito da densidade de plantio na severidade da doença, com os níveis de severidade aumentando juntamente ao aumento no número de plantas na linha de plantio, para os dois espaçamentos utilizados.(AU)

The aim of this research was to evaluate the severity of alternaria leaf spot (Alternaria helianthi) in sunflower crop sown in different densities. The experiments were conducted at the Ressacada Experimental Farm, Florianopolis, SC, Brazil, on the crop season of 2009/2010. Sowing was done at a spacing of 0.5 and 1.0 m between the rows, keeping 3.7, 4.9, 5.7 and 6.6 plants per meter, resulting in 74,000; 98,000; 114,000 and 132,000 plants per hectare to 0.5 m between the tree rows and 37,000; 49,000; 57,000 and 66,000 plants per hectare for the spacing of 1.0 m. The experimental design was in randomized blocks in a 4 x 2 factorial (four seeding rates and two row spacings) with three replicates. The severity was assessed based on diagrammatic scale for specific alternaria leaf spot in sunflower. The results indicated no interaction between the levels of severity with the variation of line spacing and distribution of plants on line, but showed effect of the distribution of plants on line in the severity, with the higher plant density in the line showed a higher severity, for the both spacing in the lines planting.(AU)

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Chlosyne lacinia saundersii is one of the most important pests of sunflower and it is the main target of insecticides applications. Larvae were collected in Londrina (PR), Santa Maria (RS), Dourados (MS), Ribeirão Preto (SP), Brasília (DF), Barreiras (BA), Uberaba (MG) and Vilhena (RO). Genomic DNA was extracted and amplified with ten-mer primers, which produced 101 loci. The size of the RAPD amplicons ranged from 180 to 2564 bp. Polymorphism among populations ranged from 31% to 67%, with the highest polymorphisms of 57% and 67% being detected in Uberaba and Vilhena populations, respectively. Populations with the highest similarity determined with Dice coefficient were from Ribeirão Preto and Barreiras, while insects from Londrina showed the highest similarity among them. Gene flow of C. lacinia saundersii 1.1 was lower than those previously observed for the noctuid Anticarsia gemmatalis Hübner, suggesting that C. lacinia saundersii populations are more isolated than the ones of this noctuid. Through the Analysis of Molecular Variance (AMOVA), RAPD variance was 33.64% among geographical populations and 66.36% within populations. These results suggest that populations of C. lacinia saundersii are genetically structured.

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RESUMO

Chlosyne lacinia saundersii is one of the most important pests of sunflower and it is the main target of insecticides applications. Larvae were collected in Londrina (PR), Santa Maria (RS), Dourados (MS), Ribeirão Preto (SP), Brasília (DF), Barreiras (BA), Uberaba (MG) and Vilhena (RO). Genomic DNA was extracted and amplified with ten-mer primers, which produced 101 loci. The size of the RAPD amplicons ranged from 180 to 2564 bp. Polymorphism among populations ranged from 31 percent to 67 percent, with the highest polymorphisms of 57 percent and 67 percent being detected in Uberaba and Vilhena populations, respectively. Populations with the highest similarity determined with Dice coefficient were from Ribeirão Preto and Barreiras, while insects from Londrina showed the highest similarity among them. Gene flow of C. lacinia saundersii 1.1 was lower than those previously observed for the noctuid Anticarsia gemmatalis Hübner, suggesting that C. lacinia saundersii populations are more isolated than the ones of this noctuid. Through the Analysis of Molecular Variance (AMOVA), RAPD variance was 33.64 percent among geographical populations and 66.36 percent within populations. These results suggest that populations of C. lacinia saundersii are genetically structured.

Chlosyne lacinia saundersii é uma das mais importantes pragas da cultura do girassol e o principal alvo das aplicações de inseticidas. As larvas foram coletadas em Londrina (PR), Santa Maria (RS), Dourados (MS), Ribeirão Preto (SP), Brasília (DF), Barreiras (BA), Uberaba (MG) e Vilhena (RO). O DNA genômico foi extraído e amplificado com dez primers, que produziram 101 locos. O tamanho das amplificações de RAPD variou de 180 a 2564 pb. O polimorfismo entre as populações variou de 31 por cento a 67 por cento, com maior polimorfismo 57 por cento e 67 por cento, detectado em populações de Uberaba e Vilhena, respectivamente. As populações com maior similaridade determinada com o coeficiente de Dice foram de Ribeirão Preto e Barreiras, enquanto os insetos coletados em Londrina apresentaram maior similaridade entre eles. O fluxo gênico de C. lacinia saundersii de 1,1 foi menor que o observado para a Anticarsia gemmatalis Hübner Noctuidae, sugerindo que as populações de C. lacinia saundersii estão mais isoladas do que estes noctuideos. Através da análise de variância molecular (AMOVA), RAPD a variação foi de 33,64 por cento entre as populações geográficas e 66,36 por cento dentro das populações. Estes resultados sugere que as populações de C. lacinia saundersii são geneticamente estruturadas.

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Avaliou-se o uso de inseticidas em tratamento de sementes de girassol no controle das pragas Cerotoma arcuatus (Olivier) (Coleoptera: Chrysomelidae), Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) e Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) e a influência desses inseticidas na qualidade fisiológica das sementes, durante o armazenamento. Os resultados obtidos mostraram que tiodicarbe 600 g i.a./ 100 kg de sementes proporcionou aproximadamente 80, 100 e 85% de controle de A. gemmatalis de 1º ínstar, S. frugiperda de 2º ínstar e adultos de C. arcuatus, respectivamente. Entretanto, esse mesmo tratamento foi ineficiente no controle de lagartas de S. frugiperda de 3º ínstar e de B. tabaci. No controle de adultos de C. arcuatus, além de tiodicarbe 600 g i.a./100 kg sementes que apresentou bons resultados, tiodicarbe + imidacloprido 157,5 + 52,5 g i.a., tiametoxam 210 e 280 g i.a., imidacloprido 245 e fipronil 50 g i.a./100 kg de sementes apresentaram eficiência acima de 80% no controle dessa praga. No controle da A. gemmatalis apenas fipronil 50 g i.a./100 kg sementes e azadiractina 10 e 20 g. i.a./100 kg de sementes proporcionaram valores menores que 80% de controle, nas doses testadas, enquanto que, para o manejo da mosca-branca, nenhum dos tratamentos estudados proporcionou controle superior a 80%. Nenhum dos inseticidas avaliados nos diferentes tempos de armazenamento (até quatro meses após o tratamento) prejudicou a germinação e a emergência das sementes.

The aim of this study was to test various insecticides in the treatment of sunflower seeds for the control of Cerotoma arcuatus (Olivier) (Coleoptera: Chrysomelidae), Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) and Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), and assessing their influence on the physiological quality of seeds during storage. The results showed that tiodicarb 600 g.a.i./100 kg of seeds achieved close to 80, 100 and 85% of control against first-instar A. gemmatalis, second instar S. frugiperda and adult C. arcuatus, respectively. However, this treatment was inefficient against third instar S. frugiperda and B. tabaci. Tiodicarb + imidacloprid 157.5 + 52.5; thiamethoxan 210; thiamethoxan 280; imidacloprid 245 and fipronil 50 g.a.i./100 kg of seeds also achieved 80% control against C. arcuatus adults. Against A. gemmatalis, only fipronil 50 g.a.i. and azarachtin 10 and 20 g.a.i./100 kg of seeds did not achieve more than 80% control. Against B. tabaci no tested treatment reached 80% of efficacy. None of the tested insecticides harmed seed germination or emergence during the different storage periods (up to 4 months after seed treatment).

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