RESUMO
Porcine reproductive and respiratory syndrome viruses (PRRSV) present a wide phenotypic and genetic diversity. Experimental infections have demonstrated viral replication, including highly pathogenic strains (HP-PRRSV), in primary lymphoid organs such as the thymus. However, studies of the bone marrow are scarce but necessary to help elucidate the immunobiology of PRRSV strains of differing virulence. In this study, whereas viral RNA was detected within the bone marrow of animals experimentally infected with both low virulent Lelystad (LV) and 215-06 PRRSV-1 strains and with the highly virulent SU1-bel strain, PRRSV positive cells were only occasionally detected in one SU1-bel infected animal. PRRSV RNA levels were associated to circulating virus with the highest levels detected in LV-infected pigs. At 3 dpi, a decrease in the proportion of haematopoietic tissue and number of erythroid cells in all infected groups was associated with an increase in TUNEL or cleaved caspase 3 labelling and higher counts of myeloid cells compared to control. The expression of IL-1α and IL-6 was elevated at the beginning of the infection in all infected animals. The expression of TNF-α was increased at the end of the study in all infected groups with respect to control. Different PRRSV-1 strains induced, presummably by indirect mechanisms and independently of viral load and strain virulence, moderate and sustained hypoplasia of erythroid cells and myeloid cell hyperplasia at early stages of infection. These changes were paralleled by a peak in the local expression of IL-1α, IL-6 and TNF-α in all infected groups.
Assuntos
Síndrome Respiratória e Reprodutiva Suína/patologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/patogenicidade , Animais , Medula Óssea/patologia , Medula Óssea/virologia , Citocinas/imunologia , Células Eritroides/patologia , Células Eritroides/virologia , Hiperplasia/patologia , Hiperplasia/veterinária , Hiperplasia/virologia , Masculino , Células Mieloides/patologia , Células Mieloides/virologia , Síndrome Respiratória e Reprodutiva Suína/virologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/fisiologia , Suínos , Carga Viral , Virulência , Replicação ViralRESUMO
Focal epithelial hyperplasia (FEH) is a rare infection caused by human papillomavirus (HPV) types 13 or 32 believed to infect exclusively oral mucosa. This report illustrates a case of multiple conjunctival papillomas similar to oral FEH caused by HPV-13, consisting in the first description of its infection outside the oral mucosa in a healthy patient.
Assuntos
Doenças da Túnica Conjuntiva/virologia , Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus , Criança , Feminino , Humanos , Hiperplasia/virologia , Papiloma/patologia , Papiloma/virologia , Infecções por Papillomavirus/epidemiologia , Infecções por Papillomavirus/patologia , Infecções por Papillomavirus/virologiaRESUMO
Immunoperoxidase labeling was performed in histological sections from rat brain harvested during acute (10-30 days), clinically inapparent (90-270 days) and late (450-540 days) stages of Junin virus-induced neurological disease. In frontoparietal cortex, count of viral antigen (+) neurons peaked during the acute period (27.7+/-6.8), dropped within the intermediate (4.8+/-4.0 to 1.4+/-1.1) and increased (7.6+/-4.3) at the onset of the late neurological syndrome. In infected vs. control rats, the number of GFAP (+) astrocytes maximized during the acute stage (19+/-4 vs. 11+/-5), and from the end of the intermediate (27+/-5 vs. 21+/-5) up to the late (37+/-7 vs. 26+/-6) periods. In turn, surface density of GFAP (+) material in infected samples peaked at 0.196+/-0.066, while it failed to exceed 0.090+/-0.043 in controls. Both astrocyte hypertrophy relapsing into chronicity, as depicted by surface density, and astrocyte hyperplasia preceding the onset of the late neurological syndrome, support their pathogenic contribution to disease expression.
Assuntos
Infecções por Arenaviridae/patologia , Astrócitos/virologia , Gliose/virologia , Vírus Junin/imunologia , Neurônios/virologia , Animais , Animais Recém-Nascidos , Infecções por Arenaviridae/imunologia , Infecções por Arenaviridae/fisiopatologia , Astrócitos/imunologia , Astrócitos/patologia , Córtex Cerebral/imunologia , Córtex Cerebral/patologia , Córtex Cerebral/virologia , Doença Crônica , Proteína Glial Fibrilar Ácida/metabolismo , Gliose/imunologia , Gliose/patologia , Hiperplasia/imunologia , Hiperplasia/patologia , Hiperplasia/virologia , Imuno-Histoquímica , Vírus Junin/patogenicidade , Neurônios/imunologia , Neurônios/patologia , Ratos , Ratos WistarRESUMO
Chronic active plasmacytic gastritis (CAPG) is characterized by the presence of chronic inflammatory cell infiltrates, mainly formed by plasma cells, involving the neck of gastric glands. This lesion, as well as Ménétrier disease, has been linked to cytomegalovirus (CMV). To test this association we evaluated the foveolar/glandular (F/G) index and the presence of CMV DNA (desoxirribonucleic acid) by means of polymerase chain reaction (PCR) in 12 cases of CAPG and 13 controls. Cases exhibiting CAPG included 2 with Ménétrier disease, 6 with foveolar hyperplasia, and 3 with normal foveolar/glandular (F/G) index. None showed either lymphocytic gastritis or CMV inclusions. Three CAPG cases were associated with gastric carcinoma. The F/G index was less than 1 in all controls. Eleven out of the 12 cases with CAPG showed amplification for CMV DNA while all controls were negative. Findings suggest a very close association, probably in progressive stages, between CMV infection, CAPG, foveolar hyperplasia (with or without Ménétrier disease) and gastric carcinoma. CAPG might be a histologic marker for CMV infection in the germinative zone of the neck of gastric glands. These findings resemble those of hepatitis B virus (HBV) infection, chronic hepatitis, cirrhosis, and hepatocellular carcinoma saga.
Assuntos
Infecções por Citomegalovirus/complicações , Gastrite/virologia , Adulto , Idoso , Estudos de Casos e Controles , Doença Crônica , Feminino , Mucosa Gástrica/patologia , Mucosa Gástrica/virologia , Gastrite/patologia , Gastrite Hipertrófica/patologia , Gastrite Hipertrófica/virologia , Humanos , Hiperplasia/patologia , Hiperplasia/virologia , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da PolimeraseRESUMO
La gastritis plasmocelular crónica activa (GPCA) se caracteriza por la presencia de infiltrados inflamatorios crónicos predominantemente plasmocelulares que comprometen las células del cuello de las glándulas gástricas. Tanto la GPCA como la gastropatía de Ménétrier han sido vinculadas con el citomegalovirus (CMV). Con el propósito de probas esta asociación, se evaluó la relación fovéolo-glandular (F/G) y se determinó la presencia del AND (ácido desoxirribonucleico) del CMV mediante PCR (reacción en cadena de polimerasa) en 12 casos y 13 controles. Los ejemplos de GPCA presentaron enfermedad de Ménétrier en 2 casos; hiperplasia foveolar, en 6 casos, y relación F/G conservada, en 3 casos. En ninguno visualizaron imágenes de gastritis linfocitaria, ni alteraciones citopáticas por CMV. Tres casos se asociaron con carcinoma gástrico. La relación F/G fue menor de 1 en todos los controles. Once de los 12 casos con GPCA mostraron amplificación para le AND del CMV y los 13 controles fueron negativos. Los hallazgos sugieren una fuerte asociación, posiblemente secuencial, entre la infección por el CMV, la GPCA, la hiperplasia foveolar (con o sin desarrollo de enfermedad de Ménétrier) y el carcinoma gástrico. La GPCA podría representar un marcador histológico de la presencia de CMV en la zona germinativa de los cuellos glandulares. La propuesta es paralela a los hallazgos, las lesiones y la secuencia observables en la infección hepática por VHB (irus de hepatitis B), con el desarrollo de hepatitis crónica, cirrosis y hepatocarcinoma.
Assuntos
Humanos , Feminino , Adulto , Pessoa de Meia-Idade , Infecções por Citomegalovirus/complicações , Gastrite/virologia , Estudos de Casos e Controles , Doença Crônica , Vírus de DNA/isolamento & purificação , Mucosa Gástrica/patologia , Mucosa Gástrica/virologia , Gastrite Hipertrófica/patologia , Gastrite Hipertrófica/virologia , Gastrite/patologia , Genoma Viral , Hiperplasia/patologia , Hiperplasia/virologia , Reação em Cadeia da PolimeraseRESUMO
La gastritis plasmocelular crónica activa (GPCA) se caracteriza por la presencia de infiltrados inflamatorios crónicos predominantemente plasmocelulares que comprometen las células del cuello de las glándulas gástricas. Tanto la GPCA como la gastropatía de Ménétrier han sido vinculadas con el citomegalovirus (CMV). Con el propósito de probas esta asociación, se evaluó la relación fovéolo-glandular (F/G) y se determinó la presencia del AND (ácido desoxirribonucleico) del CMV mediante PCR (reacción en cadena de polimerasa) en 12 casos y 13 controles. Los ejemplos de GPCA presentaron enfermedad de Ménétrier en 2 casos; hiperplasia foveolar, en 6 casos, y relación F/G conservada, en 3 casos. En ninguno visualizaron imágenes de gastritis linfocitaria, ni alteraciones citopáticas por CMV. Tres casos se asociaron con carcinoma gástrico. La relación F/G fue menor de 1 en todos los controles. Once de los 12 casos con GPCA mostraron amplificación para le AND del CMV y los 13 controles fueron negativos. Los hallazgos sugieren una fuerte asociación, posiblemente secuencial, entre la infección por el CMV, la GPCA, la hiperplasia foveolar (con o sin desarrollo de enfermedad de Ménétrier) y el carcinoma gástrico. La GPCA podría representar un marcador histológico de la presencia de CMV en la zona germinativa de los cuellos glandulares. La propuesta es paralela a los hallazgos, las lesiones y la secuencia observables en la infección hepática por VHB (irus de hepatitis B), con el desarrollo de hepatitis crónica, cirrosis y hepatocarcinoma. (AU)